Nano-based remediation strategies for micro and nanoplastic pollution.

Due to rapid urbanization, there have been continuous environmental threats from different pollutants, especially from microplastics. Plastic products rapidly proliferate significantly contributing to the occurrence of micro-plastics, which poses a significant environmental risk. These microplastics originated from diverse sources and are characterized by their persistent and widespread occurrence; human health and the entire ecosystem are adversely affected by them. The removal of microplastics not only requires innovative technologies but also efficient materials capable of effectively eliminating them from our environment. The progress made so far has highlighted the advantages of utilizing the dimensional and structural properties of nanomaterials to increase the effectiveness of existing methods for micro-plastic treatment, aiming for a more sustainable approach to their removal. In the current review, we demonstrate a thorough overview of the sources, occurrences, and potential harmful effects of microplastics, followed by a further discussion of promising technologies used for their removal. An in-depth examination of both advantages and a few limitations of all these given technologies, including physical, chemical, and biological approaches, has been discussed. Additionally, the review explores the use of nanomaterials as an effective means to overcome obstacles and improve the efficiency of microplastic elimination methods. n conclusion, this review addresses, current challenges in this field and outlines the future perspectives for further research in this domain.

The potential of zeolite nanocomposites in removing microplastics, ammonia, and trace metals from wastewater and their role in phytoremediation.

Nanocomposites are emerging as a new generation of materials that can be used to combat water pollution. Zeolite-based nanocomposites consisting of combinations of metals, metal oxides, carbon materials, and polymers are particularly effective for separating and adsorbing multiple contaminants from water. This review presents the potential of zeolite-based nanocomposites for eliminating a range of toxic organic and inorganic substances, dyes, heavy metals, microplastics, and ammonia from water. The review emphasizes that nanocomposites offer enhanced mechanical, catalytic, adsorptive, and porosity properties necessary for sustainable water purification techniques compared to individual composite materials. The adsorption potential of several zeolite-metal/metal oxide/polymer-based composites for heavy metals, anionic/cationic dyes, microplastics, ammonia, and other organic contaminants ranges between approximately 81 and over 99%. However, zeolite substrates or zeolite-amended soil have limited benefits for hyperaccumulators, which have been utilized for phytoremediation. Further research is needed to evaluate the potential of zeolite-based composites for phytoremediation. Additionally, the development of nanocomposites with enhanced adsorption capacity would be necessary for more effective removal of pollutants.

Sb-Doped Cerium Molybdate: An Emerging Material as Dielectric and Photocatalyst for the Removal of Diclofenac Potassium from Aqueous Media.

This work reports the influence of antimony substitution in a cerium molybdate lattice for improved dielectric and photocatalytic properties. For this purpose, a series of Ce2-xSbx(MoO4)3 (x = 0.00, 0.01, 0.03, 0.05, 0.07, and 0.09) were synthesized through a co-precipitation route. The as-synthesized materials were characterized for their optical properties, functional groups, chemical oxidation states, structural phases, surface properties, and dielectric characteristics using UV-Vis spectroscopy (UV-Vis), Fourier transform infrared (FTIR) and Raman spectroscopies, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, and impedance spectroscopy, respectively. UV-Vis study showed a prominent red shift of absorption maxima and a continuous decrease in band gap (3.35 eV to 2.79 eV) by increasing the dopant concentration. The presence of Ce-O and Mo-O-Mo bonds, detected via FTIR and Raman spectroscopies, are confirmed, indicating the successful synthesis of the desired material. The monoclinic phase was dominant in all materials, and the crystallite size was decreased from 40.29 nm to 29.09 nm by increasing the Sb content. A significant increase in the dielectric constant (ε' = 2.856 × 108, 20 Hz) and a decrease in the loss tan (tanδ = 1.647, 20 Hz) were exhibited as functions of the increasing Sb concentration. Furthermore, the photocatalytic efficiency of pristine cerium molybdate was also increased by 1.24 times against diclofenac potassium by incorporating Sb (x = 0.09) in the cerium molybdate. The photocatalytic efficiency of 85.8% was achieved within 180 min of UV light exposure at optimized conditions. The photocatalytic reaction followed pseudo-first-order kinetics with an apparent rate constant of 0.0105 min-1, and the photocatalyst was recyclable with good photocatalytic activity even after five successive runs. Overall, the as-synthesized Sb-doped cerium molybdate material has proven to be a promising candidate for charge storage devices and a sustainable photocatalyst for wastewater treatment.

Autoimmune Heparin-Induced Thrombocytopenia: A Diagnostic and Management Challenge After Transcatheter Aortic Valve Replacement.

Heparin-induced thrombocytopenia (HIT) is a commonly encountered condition, especially in inpatient settings, and is often attributed to high mortality and prolonged hospital stays. A rare entity, autoimmune heparin-induced thrombocytopenia (aHIT) refers to a condition in which antiplatelet factor-4 (PF4) antibodies activate platelets even in the absence of heparin. Our patient presented 12 days after transcatheter aortic valve replacement (TAVR) with altered mental status and severe thrombocytopenia. Further work-up revealed acute thromboembolic cerebrovascular accident (CVA), and the HIT antibody was positive. He was started on intravenous argatroban infusion with poor response. Platelet factor-4 antibodies were positive as well, and he was started on intravenous immunoglobulins (IVIG) therapy resulting in platelet recovery. This case is a reminder to consider autoimmune HIT, especially when platelet count fails to improve with conventional therapy.

Case report of culture-negative endocarditis in lupus nephritis.

Background: Cardiovascular involvement is frequent in systemic lupus erythematosus (SLE). Valvular abnormalities are increasingly being recognized with the advent of echocardiography. Case summary: We present a case of a 46-year-old lady who presented to the emergency department with upper limb ischaemia. On examination, she had poor dentition and a short systolic murmur on auscultation. A blood workup revealed a diagnosis of SLE. Further investigations showed vegetations on the mitral valve. Initially, an infective endocarditis (IE) diagnosis was made, which was treated with antibiotics. High-dose steroids and immunosuppressants were initiated due to her clinical deterioration and biopsy-proven lupus nephritis. She improved clinically before being discharged home. Discussion: It can be difficult to distinguish between IE and Libman-Sacks endocarditis (LSE), especially in the setting of risk factors for both. Antibiotics and immunosuppressants might be started simultaneously in these cases. A multidisciplinary team is required to manage challenging cases of culture-negative endocarditis. Procalcitonin may have a role in differentiating bacterial endocarditis and LSE.

Degradation of cefadroxil drug by newly designed solar light responsive alcoholic template-based lanthanum ferrite nanoparticles.

In this work, lanthanum ferrite nanoparticles were synthesized via a simple co-precipitation method. Two different templates, namely sorbitol and mannitol, were used in this synthesis to tune the optical, structural, morphological, and photocatalytic properties of lanthanum ferrite. The synthesized lanthanum ferrite-sorbitol (LFOCo-So) and lanthanum ferrite-mannitol (LFOCo-Mo) were investigated through Ultraviolet-Visible (UV-Vis), X-ray diffraction (XRD), Fourier Transform Infra-Red (FTIR), Raman, Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX), and photoluminescence (PL) techniques to study the effects of the templates on the tunable properties of lanthanum ferrite nanoparticles. The UV-Vis study revealed a remarkably small bandgap (2.09 eV) of LFOCo-So compared to the LFOCo-Mo having a band gap of 2.46 eV. XRD analysis revealed a single-phased structure of LFOCo-So, whereas LFOCo-Mo showed different phases. The calculated crystallite sizes of LFOCo-So and LFOCo-Mo were 22 nm and 39 nm, respectively. FTIR spectroscopy indicated the characteristics of metal-oxygen vibrations of perovskites in both lanthanum ferrite (LFO) nanoparticles, whereas a slight shifting of Raman scattering modes in LFOCo-Mo in contrast to LFOCo-So showed the octahedral distortion of the perovskite by changing the template. SEM micrographs indicated porous particles of lanthanum ferrite with LFOCo-So being more uniformly distributed, and EDX confirmed the stoichiometric ratios of the lanthanum, iron, and oxygen in the fabricated lanthanum ferrite. The high-intensity green emission in the photoluminescence spectrum of LFOCo-So indicated more prominent oxygen vacancies than LFOCo-Mo. The photocatalytic efficiency of synthesized LFOCo-So and LFOCo-Mo was investigated against cefadroxil drug under solar light irradiation. At optimized photocatalytic conditions, LFOCo-So showed higher degradation efficiency of 87% in only 20 min than LFOCo-Mo having photocatalytic activity of 81%. The excellent recyclability of the LFOCo-So reflected that it could be reused without affecting photocatalytic efficiency. These findings showed that sorbitol is a useful template for the lanthanum ferrite particles imparting outstanding features, enabling it to be used as an efficient photocatalyst for environmental remediation.

Bio-fabricated bismuth-based materials for removal of emerging environmental contaminants from wastewater.

Although rapid industrialization has made life easier for humans, several associated issues are emerging and harming the environment. Wastewater is regarded as one of the key problems of the 21st century due to its massive production every year and requires immediate attention from all stakeholders to protect the environment. Since the introduction of nanotechnology, bismuth-based nanomaterials have been used in variety of applications. Various techniques, such as hydrothermal, solvo-thermal and biosynthesis, have been reported for synthesizing these materials, etc. Among these, biosynthesis is eco-friendly, cost-effective, and less toxic than conventional chemical methods. The prime focuses of this review are to elaborate biosynthesis of bismuth-based nanomaterials via bio-synthetic agents such as plant, bacteria and fungi and their application in wastewater treatment as anti-pathogen/photocatalyst for pollutant degradation. Besides this, future perspectives have been presented for the upcoming research in this field, along with concluding remarks.

Development of zinc ferrite nanoparticles with enhanced photocatalytic performance for remediation of environmentally toxic pharmaceutical waste diclofenac sodium from wastewater.

Diclofenac sodium is an anti-inflammatory drug commonly used to cure pain in various treatments. The remarkable potential of this pain-killer leads to its excessive use and, therefore, a persistent water contaminant. Its presence in aqueous bodies is hazardous for both humans and the environment because it causes the growth of harmful drug-resistant bacteria in water. Herein, we present a comparative study of the ZnO and ZnFe2O4 as photocatalysts for the degradation of diclofenac sodium, along with their structural and morphological studies. A simple co-precipitation method was used for the synthesis of ZnO and ZnFe2O4 and characterized by various analytical techniques. For instance, the UV-Vis study revealed the absorption maxima of ZnO at 320 nm, which was shifted to a longer wavelength region at 365 nm for zinc ferrite. The optical band gaps obtained from the Tauc plot indicated that the incorporation of iron has led to a decreased band gap of zinc ferrite (2.89 eV) than pure ZnO (3.14 eV). The metal-oxygen linkages shown by FTIR indicated the formation of desired ZnO and ZnFe2O4, which was further confirmed by XRD. It elucidated the typical hexagonal structure for ZnO and spinel cubic structure for ZnFe2O4 with an average crystallite of 31 nm and 44 nm for ZnO and ZnFe2O4, respectively. The micrographs obtained by SEM showed rough spherical particles of ZnO, whereas for ZnFe2O4 flower-like clustered particles were observed. The photocatalytic investigation against diclofenac sodium revealed the higher degradation efficiency of ZnFe2O4 (61.4%) in only 120 min, whereas ZnO degraded only 48.9% of the drug. Moreover, zinc ferrite has shown good recyclability and was stable up to five runs of photodegradation with a small loss (3.9%) of photocatalytic activity. The comparison of two catalysts has suggested the promising role of zinc ferrite in wastewater remediation to eliminate hazardous pharmaceuticals.

Functionalized magnetic nanostructured composites and hybrids for photocatalytic elimination of pharmaceuticals and personal care products.

Due to rapid urbanization and globalization, an enormous use of pharmaceuticals and personal care products (PPCPs) has resulted their excessive release in water bodies leading to several environmental issues. This release into the environment takes place via household sewage, hospital effluents, manufacturing units and landfill sites etc. The pharmaceuticals and personal care products (PPCPs) are recently listed as emerging contaminants having many adverse effects towards aquatic life, human beings, and the whole ecosystem. The alarming threats of PPCPs demand efficient methods to cope up their hazardous impacts. The conventional wastewater remediations are not specifically designed for the removal of PPCPs and hence, they require advanced technologies and materials for their elimination to ensure water safety. Among various methods employed so far, photocatalysis is considered to be one of the most cost effective and eco-friendly method but it requires a suitable candidate as a photocatalyst. Thanks to the magnetic nanocomposites which have improved the limitations (poor stability, agglomeration, and difficult separation, etc.) of classically used nanomaterials. Magnetic nanocomposites contain at least one component having magnetic properties making their separation easy from the aqueous media after the photodegradation phenomenon. These can be further functionalized with other materials to obtain maximum advantage as photocatalyst. Few examples of such functionalized nanocomposites are inorganic material based magnetic nanocomposites, carbon based magnetic nanocomposites, biomaterial based magnetic nanocomposites, metal-organic framework based magnetic nanocomposites and polymer based magnetic nanocomposites etc. This review covers the global environmental issue of water pollution especially with respect to the PPCPs, their occurrence in aqueous environment and toxic effects on living beings. A comprehensive discussion of the recently reported functionalized magnetic nanocomposites for the photocatalytic removal of PPCPs from water is the main aim of this review. The synthetic/morphological approaches of various functionalized magnetic composites and their mechanism of action are also elaborated. The possible research challenges in the field of magnetic nanocomposites and future research directions are discussed to apply magnetic nanocomposites for wastewater treatment in near future.

Nanoengineered metal-organic framework for adsorptive and photocatalytic mitigation of pharmaceuticals and pesticide from wastewater.

Rapidly expanding water pollution has transformed into significant dangers around the world. In recent years, the pharmaceutical and agriculture field attained enormous progress to meet the necessities of health and life; however, discharge of trace amounts of pharmaceuticals and pesticides into water significantly have a negative influence on human health and the environment. Contamination with these pollutants also constitutes a great threat to the aquatic ecosystem. To deal with the harmful impacts of such pollutants, their expulsion has attracted researchers' interest a lot, and it became essential to figure out techniques suitable for the removal of these pollutants. Thus, many researchers have devoted their efforts to improving the existing technology or providing an alternative strategy to solve this environmental problem. One of the attractive materials for this purpose is metal-organic frameworks (MOFs) due to their superior high surface area, high porosity, and the tunable features of their structures and function. Among various techniques of wastewater treatment, such as biological treatment, advanced oxidation process and membrane technologies, etc., metal-organic frameworks (MOFs) materials are tailorable porous architectures and are viably used as adsorbents or photocatalysts for wastewater treatment due to their porosity, tunable internal structure, and large surface area. MOFs are synthesized by various methods such as solvo/hydrothermal, sonochemical, microwave and mechanochemical methods. Most common method used for the synthesis of MOFs is solvothermal/hydrothermal methods. Herein, this review aims at providing a comprehensive overview of the latest advances in MOFs and their derivatives, focusing on the following aspects: synthesis and applications. This review comprehensively highlights the application of MOFs and nano-MOFs to remove pharmaceuticals and pesticides from wastewater. For the past years, transition metal-based MOFs have been concentrated as photocatalyst/adsorbents in treating contaminated water. However, work on main group metal-based MOFs is not so abundant. Hence, the foremost objective of this review is to present the latest material and references concerning main group element-based MOFs and nanoscale materials derived from them towards wastewater treatment. It summarizes the possible research challenges and directions for MOFs and their derivatives as catalysts applied to wastewater treatment in the future. With the context of recent pioneering studies on main group elements-based MOFs and their derivatives; we hope to stimulate some possibilities for further development, challenges and future perspectives in this field have been highlighted.

Identification and characterisation of blue light photoreceptor gene family and their expression in tomato (Solanum lycopersicum) under cold stress.

The Arabidopsis thaliana L. photoreceptor genes homologues in tomato (Solanum lycopersicum L.) genome were analysed using bioinformatic tools. The expression pattern of these genes under cold stress was also evaluated. Transcriptome analysis of the tomato sequence revealed that the photoreceptor gene family is involved in abiotic stress tolerance. They participate in various pathways and controlling multiple metabolic processes. They are structurally related to PAS, LIGHT-OXYGEN-VOLTAGE-SENSING (LOV), DNA photolyase, 5,10-methenyl tetrahydrofolate (MTHF), flavin-binding kelch F-box, GAF, PHY, Seven-bladed ß-propeller and C27 domains. They also interact with flavin adenine dinucleotide (FAD), (5S)-5-methyl-2-(methylsulfanyl)-5-phenyl-3-(phenylamino)-3,5-dihydro-4H-imidazol-4-one (FNM) and Phytochromobilin (PϕB) ligands. These interactions help to create a cascade of protein phosphorylation involving in cell defence transcription or stress-regulated genes. They localisation of these gene families on tomato chromosomes appeared to be uneven. Phylogenetic tree of tomato and Arabidopsis photoreceptor gene family were classified into eight subgroups, indicating gene expression diversity. Morphological and physiological assessment revealed no dead plant after 4h of cold treatment. All the plants were found to be alive, but there were some variations in the data across different parameters. Cold stress significantly reduced the rate of photosynthesis from 10.06 to 3.16µmolm-2 s-1 , transpiration from 4.6 to 1.3mmolm-2 s-1 , and stomatal conductance from 94.6 to 25.6mmolm-2 s-1 . The cold stressed plants also had reduced height, root/shoot length, and fresh/dry biomass weight than the control plants. Relative expression analysis under cold stress revealed that after 4h, light stimulates the transcript level of Cry2 from 1.9 to 5.7 and PhyB from 0.98 to 6.9 compared to other photoreceptor genes.

MXene-based hybrid composites as photocatalyst for the mitigation of pharmaceuticals.

Environmental contamination is a burning issue and has gained global attention in the present era. Pharmaceuticals are emerging contaminants affecting the natural environment worldwide owing to their extensive consumption particularly in developing countries where self-medication is a common practice. These pharmaceuticals or their degraded active metabolites enter water bodies via different channels and are continuous threat to the whole ecological system. There is a dire need to find efficient approaches for their removal from all environmental matrices. Photocatalysis is one of the most effective and simple approach, however, finding a suitable photocatalyst is a challenging task. Recently, MXenes (two-dimensional transition metal carbides/nitrides), a relatively new material has attracted increasing interest as photocatalysts due to their exceptional properties, such as large surface area, appreciable safety, huge interlayer spacing, thermal conductivity, and environmental flexibility. This review describes the recent advancements of MXene-based composites and their photocatalytic potential for the elimination of pharmaceuticals. Furthermore, present limitations and future research requirements are recommended to attain more benefits of MXene-based composites for the purification of wastewater.

Transporters and transcription factors gene families involved in improving nitrogen use efficiency (NUE) and assimilation in rice (Oryza sativa L.).

Nitrogen (N) as a macronutrient is an important determinant of plant growth. The excessive usage of chemical fertilizers is increasing environmental pollution; hence, the improvement of crop's nitrogen use efficiency (NUE) is imperative for sustainable agriculture. N uptake, transportation, assimilation, and remobilization are four important determinants of plant NUE. Oryza sativa L. (rice) is a staple food for approximately half of the human population, around the globe and improvement in rice yield is pivotal for rice breeders. The N transporters, enzymes indulged in N assimilation, and several transcription factors affect the rice NUE and subsequent yield. Although, a couple of improvements have been made regarding rice NUE, the knowledge about regulatory mechanisms operating NUE is scarce. The current review provides a precise knowledge of how rice plants detect soil N and how this detection is translated into the language of responses that regulate the growth. Additionally, the transcription factors that control N-associated genes in rice are discussed in detail. This mechanistic insight will help the researchers to improve rice yield with minimized use of chemical fertilizers.

Nanohybrids-assisted photocatalytic removal of pharmaceutical pollutants to abate their toxicological effects - A review.

Advancement in medication by health care sector has undoubtedly improved our life but at the same time increased the chemical burden on our natural ecosystem. The residuals of pharmaceutical products become part of wastewater streams by different sources such as excretion after their usage, inappropriate way of their disposal during production etc. Hence, they are serious health hazards for human, animal, and aquatic lives. Due to rapid urbanization, the increased demand for clean drinking water is a burning global issue. In this regard it is need of the present era to explore efficient materials which could act as photocatalyst for mitigation of pharmaceuticals in wastewater. Nanohybrid as photocatalyst is one of the widely explored class of materials in photocatalytic degradation of such harmful pollutants. Among these nanohybrids; metal based nanohybrids (metals/metal oxides) and carbon based nanohybrids (carbon nanotubes, graphene, fullerenes etc.) have been explored to remove pharmaceutical drugs. Keeping in view the increasing harmful impacts of pharmaceuticals; the sources of pharmaceuticals in wastewater, their health risk factors and their mitigation using efficient nanohybrids as photocatalysts have been discussed in this review.

A gain-of-function mutation of the MATE family transporter DTX6 confers paraquat resistance in Arabidopsis.

Paraquat is one of the most widely used nonselective herbicides and has elicited the emergence of paraquat-resistant weeds. However, the molecular mechanisms of paraquat resistance are not completely understood. Here we report the Arabidopsis gain-of-function mutant pqt15-D with significantly enhanced resistance to paraquat and the corresponding gene PQT15, which encodes the Multidrug and Toxic Extrusion (MATE) transporter DTX6. A point mutation at +932 bp in DTX6 causes a G311E amino acid substitution, enhancing the paraquat resistance of pqt15-D, and overexpression of DTX6/PQT15 in the wild-type plants also results in strong paraquat resistance. Moreover, heterologous expression of DTX6 and DTX6-D in Escherichia coli significantly enhances bacterial resistance to paraquat. Importantly, overexpression of DTX6-D enables Arabidopsis plants to tolerate 4 mM paraquat, a near-commercial application level. DTX6/PQT15 is localized in the plasma membrane and endomembrane, and functions as a paraquat efflux transporter as demonstrated by paraquat efflux assays with isolated protoplasts and bacterial cells. Taken together, our results demonstrate that DTX6/PQT15 is an efflux transporter that confers paraquat resistance by exporting paraquat out of the cytosol. These findings reveal a molecular mechanism of paraquat resistance in higher plants and provide a promising candidate gene for engineering paraquat-resistant crops.

Biotic stress triggered small RNA and RNAi defense response in plants.

The yield of crops is largely affected by different types of biotic stresses. To minimize the damage, crop plants adapted themselves to overcome the stress conditions through gene expression reprogramming at transcriptional and post-transcriptional levels. With a better knowledge of plants' responses in adverse environments, new methodologies and strategies have been applied to develop better stress-tolerant plants. In this manner, small RNAs (micro RNA and small-interfering RNA) are reported to play a central role to combat biotic stresses in plants. Depending upon the stress stimuli, these small RNAs can up or down regulate the genes expression, that indicate their potential role in overcoming the stress. These stress-induced small RNAs may reduce the expression of the target gene(s) that might negatively influence plants' response to the adverse conditions. Contrariwise, miRNA, a class of small RNA, can downregulate its expression to upregulate the expression of the target gene(s), which might positively aid to the stress adaptation. Along with this, benefits of RNA interference (RNAi) have also been stated in functional genomic research on insects, fungi and plant pathogens. RNAi is involved in the safe transport of dsRNA to the targeted mRNA(s) in the biotic stress-causing agents (for example fungi and insects) and saves the plant from damage, which is a safer approach compared to use of chemical pesticides. The current review summarizes the role of small RNAs and the use of RNAi to save the plants from biotic stress conditions.

Harboyan Syndrome.

Harboyan syndrome is a degenerative corneal disorder characterized by congenital hereditary endothelial dystrophy along with progressive, post lingual sensorineural hearing loss. We present the case of a 16-year-old female, who came to us for her follow up after surgical correction for bilateral corneal opacities (bilateral keratplasy) and use of hearing aid for SNHL. Her symptoms resolved significantly. Currently, she's living a healthy life after being treated for Harboyan syndrome. Physical examination and laboratory investigations ruled out other causes (eg: Congenital cataracts, Peters anomaly, Sclerocornea) and the diagnosis of Harboyan syndrome was established.

Effect of calcium hydroxide on mechanical strength and biological properties of bioactive glass.

In this manuscript for the first time calcium hydroxide (Ca(OH)2) has been used for preparation of bioactive glass (BG-2) by co-precipitation method and compared with glass prepared using calcium nitrate tetrahydrate Ca(NO3)2·4H2O (BG-1), which is a conventional source of calcium. The new source positively affected physical, biological and mechanical properties of BG-2. The glasses were characterized by Fourier transform infrared (FTIR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis/Differential Scanning Calorimetry (TGA-DSC), BET surface area analysis and Knoop hardness. The results showed that BG-2 possessed relatively larger surface properties (100m(2)g(-1) surface area) as compared to BG-1 (78m(2)g(-1)), spherical morphology and crystalline phases (wollastonite and apatite) after sintering at lower than conventional temperature. These properties contribute critical role in both mechanical and biological properties of glasses. The Knoop hardness measurements revealed that BG-2 possessed much better hardness (0.43±0.06GPa at 680°C and 2.16±0.46GPa at 980°C) than BG-1 (0.24±0.01 at 680°C and 0.57±0.07GPA at 980°C) under same conditions. Alamar blue Assay and confocal microscopy revealed that BG-2 exhibited better attachment and proliferation of MG63 cells. Based on the improved biological properties of BG-2 as a consequent of novel calcium source selection, BG-2 is proposed as a bioactive ceramic for hard tissue repair and regeneration applications.