Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Bioorg Med Chem Lett ; 26(23): 5624-5630, 2016 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-27825544

RESUMO

A series of fifteen new chemical entities, 3-(pyridin-4-yl)-1H-pyrazole-5-carboxamide chalcones (6a-o), were synthesized as new hybrids with enriched biological activities compared to their parent molecules. The compounds were characterized by 1H NMR, 13C NMR, Mass and IR spectral studies. Their antibacterial, anti-inflammatory and antioxidant activities have been evaluated. These compounds showed moderate to good antibacterial, anti-inflammatory and antioxidant activities. The molecular docking analysis was performed with cyclooxygenase enzyme to ascertain the probable binding model.


Assuntos
Chalcona/química , Chalcona/farmacologia , Pirazóis/química , Pirazóis/farmacologia , Antibacterianos/química , Antibacterianos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Infecções Bacterianas/tratamento farmacológico , Ciclo-Oxigenase 1/metabolismo , Ciclo-Oxigenase 2/metabolismo , Inibidores de Ciclo-Oxigenase/química , Inibidores de Ciclo-Oxigenase/farmacologia , Descoberta de Drogas , Humanos , Simulação de Acoplamento Molecular
2.
Biosens Bioelectron ; 79: 121-9, 2016 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-26703990

RESUMO

In this report, we have successfully fabricated an immunosensor for detection of Pseudomonas aeruginosa in water. The monoclonal antibody was immobilized on the surface modified with CCLP (Calcium Cross-Linked Pectin)-Au NPs (gold nanoparticles)/Glassy Carbon Electrode. The building of the immunosensor was evaluated in each step by cyclic voltammetry (CV) and impedance spectroscopy (EIS). The electrochemical detection was done based on the anti rabbit IgG HRP (Horseradish Peroxidase) which binds to the immune complex and the response was observed using Hydro Quininone (HQ) and Hydrogen peroxide (H2O2) in PB (Phosphate Buffer) electrolyte. From the results, the sensitivity range is from 10(1) to 10(7)CFU/ml and LOD is calculated as 9×10(2)CFU/ml. The developed immunosensor also have high selectivity, stability, reproducibility and reusability.


Assuntos
Ouro/química , Nanocompostos/química , Pectinas/química , Pseudomonas aeruginosa/isolamento & purificação , Microbiologia da Água , Animais , Anticorpos Imobilizados/química , Espectroscopia Dielétrica/métodos , Técnicas Eletroquímicas/métodos , Peroxidase do Rábano Silvestre/química , Peróxido de Hidrogênio/química , Imunoensaio/métodos , Limite de Detecção , Nanocompostos/ultraestrutura , Coelhos , Reprodutibilidade dos Testes
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA