RESUMO
With the recent increase in intelligent CCTVs for visual surveillance, a new image degradation that integrates resolution conversion and synthetic rain models is required. For example, in heavy rain, face images captured by CCTV from a distance have significant deterioration in both visibility and resolution. Unlike traditional image degradation models (IDM), such as rain removal and super resolution, this study addresses a new IDM referred to as a scale-aware heavy rain model and proposes a method for restoring high-resolution face images (HR-FIs) from low-resolution heavy rain face images (LRHR-FI). To this end, a two-stage network is presented. The first stage generates low-resolution face images (LR-FIs), from which heavy rain has been removed from the LRHR-FIs to improve visibility. To realize this, an interpretable IDM-based network is constructed to predict physical parameters, such as rain streaks, transmission maps, and atmospheric light. In addition, the image reconstruction loss is evaluated to enhance the estimates of the physical parameters. For the second stage, which aims to reconstruct the HR-FIs from the LR-FIs outputted in the first stage, facial component-guided adversarial learning (FCGAL) is applied to boost facial structure expressions. To focus on informative facial features and reinforce the authenticity of facial components, such as the eyes and nose, a face parsing-guided generator and facial local discriminators are designed for FCGAL. The experimental results verify that the proposed approach based on a physical-based network design and FCGAL can remove heavy rain and increase the resolution and visibility simultaneously. Moreover, the proposed heavy rain face image restoration outperforms state-of-the-art models of heavy rain removal, image-to-image translation, and super resolution.
Assuntos
Processamento de Imagem Assistida por Computador , Chuva , Processamento de Imagem Assistida por Computador/métodosRESUMO
AIM: The aim was to study whether oral glucosamine hydrochloride (GlcN.HCl) or mucopolysaccharide protein (MucoP) has a structure-modifying effect on an anterior cruciate ligament transection (ACLT) rabbit model of osteoarthritis (OA). METHODS: OA was surgically induced in the right knees of rabbits by transection of the ACLT. The left knees served as a sham-operated control. The animals were divided into four groups (n = 6 each): negative control (phosphate buffered saline, orally), positive control (oral celecoxib 10 mg/kg body weight/day), GlcN.HCl (oral 100 mg/kg/day) and MucoP (oral 100 mg/kg/day). Experimental animals were sacrificed after 8 weeks of treatment and the distal femur was removed for macroscopic examination, histological assessment, and terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) assay of the OA rabbits. RESULTS: On gross morphology, severe lesions were observed in articular cartilage in the negative control group. In the GlcN.HCl and MucoP treatment groups, fibrillations and cartilaginous lesions were significantly (P < 0.05) decreased compared to the negative control group. In particular, degenerative changes in cartilage and chondrocyte cellularity were significantly reduced (P < 0.05) in the positive control (celecoxib) group, GlcN.HCl treatment group and MucoP treatment group compared with the negative control group. TUNEL assay showed that apoptotic chondrocytes were significantly suppressed in the celecoxib group. Similar significant (P < 0.05) results were seen in the GlcN.HCl group and MucoP group but apoptosis of chondrocytes were high in the negative control group. CONCLUSION: These data suggest that the protective effects of GlcN.HCl and MucoP may play a useful role in the clinical treatment of OA.
Assuntos
Cartilagem Articular/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Glucosamina/administração & dosagem , Glicosaminoglicanos/administração & dosagem , Articulações/efeitos dos fármacos , Osteoartrite/tratamento farmacológico , Administração Oral , Animais , Ligamento Cruzado Anterior/cirurgia , Apoptose/efeitos dos fármacos , Cartilagem Articular/diagnóstico por imagem , Cartilagem Articular/patologia , Celecoxib/farmacologia , Condrócitos/patologia , Inibidores de Ciclo-Oxigenase 2/farmacologia , Modelos Animais de Doenças , Membro Posterior , Articulações/diagnóstico por imagem , Articulações/patologia , Osteoartrite/patologia , Coelhos , Fatores de TempoRESUMO
Proteomic analyses have already been used in a number of hepatological studies and provide important information. However, few reports have focused on changes in the cytoplasmic proteome. The present study therefore aimed to evaluate changes in cytoplasmic proteome of rats in response to alcoholic hepatotoxicity. Rats were fed a Liber-DeCarli liquid diet containing ethanol for four weeks. Cytoplasmic proteins except mitochondrial proteins from the livers of these animals were investigated using two-dimensional gel electrophoresis and mass spectrometry. Alcohol induced a decrease in body weight gain and an increase in alanine transaminase (ALT), cholesterol, and phospholipid levels. Histopathological observations revealed hepatic damage characterized by necrosis and fatty change in alcohol-treated group at week 2, which continues until week 4. Our proteomic analysis revealed that 25 proteins were differentially expressed in the ethanol-fed group. Of these, 12 cytoplasmic proteins are being reported for the first time. Taken together, our results provide further insights into the disease mechanism and therapeutic information of alcoholic liver disease.
Assuntos
Citoplasma/patologia , Hepatopatias Alcoólicas/patologia , Fígado/patologia , Proteoma/análise , Alanina Transaminase/sangue , Animais , Peso Corporal , Colesterol/sangue , Citoplasma/metabolismo , Eletroforese em Gel Bidimensional , Etanol/toxicidade , Fígado/metabolismo , Hepatopatias Alcoólicas/sangue , Hepatopatias Alcoólicas/metabolismo , Masculino , Proteoma/metabolismo , Proteômica , Ratos WistarRESUMO
Smad3 is a key mediator of the transforming growth factor (TGF)-ß1 signaling pathway that plays central role in inflammation and fibrosis. In present study, we evaluated the effect of Smad3 deficiency in Smad3-/- mice with carbon tetrachloride (CCl4)-induced liver fibrosis. The animals were received CCl4 or olive oil three times a week for 4 weeks. Histopathological analyses were performed to evaluate the fibrosis development in the mice. Alteration of protein expression controlled by Smad3 was examined using a proteomic analysis. CCl4-induced liver fibrosis was rarely detected in Smad3-/- mice compared to Smad3+/+. Proteomic analysis revealed that proteins related to antioxidant activities such as senescence marker protein-30 (SMP30), selenium-binding proteins (SP56) and glutathione S-transferases (GSTs) were up-regulated in Smad3-/- mice. Western blot analysis confirmed that SMP30 protein expression was increased in Smad3-/- mice. And SMP30 levels were decreased in CCl4-treated Smad3+/+ and Smad3-/- mice. These results indicate that Smad3 deficiency influences the proteins level related to antioxidant activities during early liver fibrosis. Thus, we suggest that Smad3 deteriorate hepatic injury by inhibitor of antioxidant proteins as well as mediator of TGF-ß1 signaling.
Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteína Smad3/genética , Animais , Tetracloreto de Carbono/toxicidade , Eletroforese em Gel Bidimensional , Glutationa Transferase/metabolismo , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/metabolismo , Cirrose Hepática/patologia , Masculino , Camundongos , Camundongos Knockout , Proteômica , Receptores de Superfície Celular/metabolismo , Índice de Gravidade de Doença , Transdução de Sinais , Proteína Smad3/deficiência , Proteína Smad3/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Fator de Crescimento Transformador beta1/metabolismo , Regulação para CimaRESUMO
In this study, we investigated the effects of the ethanol extract of aerial parts of Raphanus sativus L. (ERL) on breast cancer cell proliferation and gene expression associated with cell proliferation and apoptosis in MDA-MB-231 human breast cancer cells. The MDA-MB-231 cells were cultured in the presence or absence of various concentrations (100, 200, or 300 µg/mL) of ERL. ERL significantly decreased cell proliferation after 48 h of incubation (P < 0.05). The protein and mRNA expression of ErbB(2) were decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of ErbB(3) was decreased significantly at an ERL concentration of 300 µg/mL (P < 0.05), and mRNA expression of ErbB(3) was decreased significantly in a dose-dependent manner (P < 0.05). The protein expression of Akt was decreased significantly at the ERL concentration of 200 µg/mL (P < 0.05), and the protein expression of pAkt was decreased significantly in a dose-dependent manner (P < 0.05). The mRNA expression of Akt was decreased significantly at the ERL concentration of 200 µg/mL ERL (P < 0.05). The protein and mRNA expression of Bax were increased significantly at ERL concentrations of 200 µg/mL or higher (P < 0.05). The protein expression of Bcl(2) was increased significantly at ERL concentrations of 100 µg/mL or higher (P < 0.05), and mRNA expression of Bcl(2) was increased significantly at an ERL concentration of 300 µg/mL (P < 0.05). In conclusion, we suggest that Raphanus sativus, L. inhibits cell proliferation via the ErbB-Akt pathway in MDA-MB-231 cells.
RESUMO
ENA Actimineral Resource A (ENA-A) is alkaline water that is composed of refined edible cuttlefish bone and two different species of seaweed, Phymatolithon calcareum and Lithothamnion corallioides. In the present study, ENA-A was investigated as an antioxidant to protect against CCl(4)-induced oxidative stress and hepatotoxicity in rats. Liver injury was induced by either subacute or chronic CCl(4) administration, and the rats had free access to tap water mixed with 0% (control group) or 10% (v/v) ENA-A for 5 or 8 weeks. The results of histological examination and measurement of antioxidant activity showed that the reactive oxygen species production, lipid peroxidation, induction of CYP2E1 were decreased and the antioxidant activity, including glutathione and catalase production, was increased in the ENA-A groups as compared with the control group. On 2-DE gel analysis of the proteomes, 13 differentially expressed proteins were obtained in the ENA-A groups as compared with the control group. Antioxidant proteins, including glutathione S-transferase, kelch-like ECH-associated protein 1, and peroxiredoxin 1, were increased with hepatocyte nuclear factor 3-beta and serum albumin precursor, and kininogen precursor decreased more in the ENA-A groups than compared to the control group. In conclusion, our results suggest that ENA-A does indeed have some protective capabilities against CCl(4)-induced liver injury through its antioxidant function.
Assuntos
Antioxidantes/farmacologia , Tetracloreto de Carbono/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Minerais/farmacologia , Preparações de Plantas/farmacologia , Animais , Catalase/metabolismo , Citocromo P-450 CYP2E1/metabolismo , Eletroforese em Gel Bidimensional , Indução Enzimática/efeitos dos fármacos , Glutationa/metabolismo , Glutationa Transferase/metabolismo , Imuno-Histoquímica , Peptídeos e Proteínas de Sinalização Intracelular , Proteína 1 Associada a ECH Semelhante a Kelch , Peroxidação de Lipídeos/efeitos dos fármacos , Espectrometria de Massas , Estresse Oxidativo/efeitos dos fármacos , Peroxirredoxinas/metabolismo , Proteínas/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismoRESUMO
Histopathological findings of eosinophilic myositis in the carcass of a slaughtered Korean native cow are presented. Lesions contained massive fibrous septae with vacuolar changes in some lesions, and the hypercontraction and rupturing of muscle bundles, with replacement by eosinophils. Necrosis and severe eosinophil infiltration were observed. Sarcoplasmic fragmentation and atrophy developed. Typical of granuloma, calcified myofibers were focally surrounded by macrophages and numerous inflammatory cells, and multinucleated giant cell formation was evident.
Assuntos
Doenças dos Bovinos/patologia , Eosinofilia/veterinária , Músculo Esquelético/patologia , Doenças Musculares/veterinária , Animais , Bovinos , Eosinofilia/patologia , Feminino , Doenças Musculares/patologiaRESUMO
Apoptosis occurs early after irradiation and may be a good indicator of radiation damages. Since elevated levels of TGF-beta are associated with radiation-induced inflammation, the null mice of Smad3, a key downstream mediator of TGF-beta, show accelerated healing of irradiated injury. In order to evaluate resistance to radiation-induced liver injuries in Smad3-null mice, we determined the occurrence of apoptosis and the expression of senescence marker protein-30 (SMP30), as an anti-apoptotic marker, after irradiation to the liver. The livers of Smad3-mutant mice were exposed to local irradiation of 15 gray, from a (60)Co-gamma radiation. One week after irradiation, in Smad3-KO mice, radiation-induced apoptosis was at lower levels compared to those of irradiated WT mice. These findings were well matched with the expression of CYP2E1, which plays a role in hepatic injuries produced by oxidative stress. In addition, antioxidant related protein, the SMP30 levels were reduced by gamma irradiation in both groups. Interestingly, the increased expression of SMP30 expression in Smad3-KO mice liver was preserved at a higher level than that of the WT mice after irradiation. Therefore, these results suggest that the interruption of TGF-beta signaling by deletion of Smad3 brings about inhibition of hepatic apoptosis after ionizing irradiation. Moreover, the protective effect to ionizing radiation might be in correlation with the overexpression of SMP30 in the Smad3-null mice, which may act as an anti-apoptotic signaling molecule. The alteration of SMP30 by interruption of Smad3 might be a useful therapeutic target and diagnostic marker for radiation-induced liver damages.
Assuntos
Apoptose/fisiologia , Apoptose/efeitos da radiação , Proteínas de Ligação ao Cálcio/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Fígado/metabolismo , Fígado/efeitos da radiação , Proteína Smad3/metabolismo , Animais , Fígado/citologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Doses de Radiação , Tolerância a Radiação/fisiologia , Proteína Smad3/genética , Regulação para Cima/efeitos da radiaçãoRESUMO
Arazyme is a novel protease produced by the HY-3 strain of Aranicola proteolyticus, which is a Gram-negative aerobic bacterium that has been isolated from the intestine of the spider Nephila clavata. This study focused on the hepatoprotective effect of Arazyme on carbon tetrachloride (CCl4)-induced acute hepatic injury in senescence marker protein 30 (SMP30) knock-out (KO) mice and SMP30 wild-type (WT) mice. WT mice and SMP30 KO mice were divided into eight groups as follows: (i) two negative control groups (G1, G5) which were treated with a single intraperitoneal (i.p.) olive oil injection. (ii) Two positive control groups (G2, G6) which received a single i.p. CCl4 (0.4mL/kg) injection. (iii) Two vitamin C-treated groups (G3, G7) which received a single oral administration of vitamin C (100mg/kg) and were injected with a single i.p. CCl4 (0.4mL/kg). (iv) Two Arazyme-treated groups (G4, G8) which received a single oral administration of Arazyme (500mg/kg) and were injected with a single i.p. CCl4 (0.4mL/kg). Through present study, we could find that Arazyme-treated groups showed decreased degree of liver injury, increased expression of SMP30, decreased expression of phospho-Smad3 (p-Smad3), elevated expression of antioxidant proteins including sorbitol dehydrogenase, dihydropteridine reductase (DHPR), dehydrofolate reductase (DHFR), NADH dehydrogenase, glutathione S-transferase kappa 1 (GSTK1) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) compared with non-Arazyme-treated groups. Therefore, it is concluded that Arazyme plays a significant role in protecting injured hepatocytes by increasing the expression of SMP30, inhibiting the transforming growth factor-beta (TGF-beta)/Smad pathway and elevating the expression of antioxidant proteins.
Assuntos
Proteínas de Ligação ao Cálcio/genética , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Peptídeos e Proteínas de Sinalização Intracelular/genética , Fígado/efeitos dos fármacos , Metaloproteases/farmacologia , Animais , Antioxidantes/farmacologia , Ácido Ascórbico/farmacologia , Proteínas de Ligação ao Cálcio/metabolismo , Intoxicação por Tetracloreto de Carbono , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Quimioprevenção , Modelos Animais de Doenças , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Técnicas Imunoenzimáticas , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Fígado/patologia , Masculino , Camundongos , Camundongos Knockout , Oxirredutases/metabolismo , Proteômica , Serratia/enzimologia , Proteína Smad3/metabolismo , Organismos Livres de Patógenos Específicos , Fator de Crescimento Transformador beta/metabolismo , Regulação para CimaRESUMO
Helicobacter pylori vacuolating cytotoxin A (VacA) has been considered as an apoptosis-inducing factor. Here, we investigated the mechanism of VacA-induced apoptosis in relation to the defense mechanism and MAP kinases pathway in gastric epithelial cells. AGS cells exposed to enriched VacA extracts affected the level of SOD-1 and villin. We further investigated the role of VacA in those inductions using a functional recombinant VacA (rVacA). Activation of p38 MAPK and Bax dimerization by rVacA were increased in a dose-dependent manner. rVacA-induced ERK1/2 MAPK activation was maximal at 30 min and 4 h and 1-4 microg/ml of rVacA. rVacA-induced SOD-1 expression was considerably diminished by inhibiting ERK1/2 MAPK and it was slightly increased by inhibiting p38 MAPK. rVacA increased or decreased villin expression depending on dose and exposure time and its expression was mainly appeared in the contractile actin ring of the dividing cells. Despite its cytoprotective effect, SB-203580, a p38 inhibitor, was unlikely to reduce VacA-induced Bax dimerization and rather inhibited villin and Bcl2 expression, indicating that p38 may also play a role in cell proliferation or differentiation for survival after VacA intoxication. Furthermore, p38 inhibitor accelerated rVacA-induced cell death after exposure of AGS cells to H(2)O(2) but ERK1/2 inhibitor protected cells from H(2)O(2) insult. These results suggest that SOD-1 and villin are expressed differentially upon VacA insult depending on dose and exposure time via ERK and p38 MAP kinases; decrease in SOD-1 and villin expression coupled with Bax dimerization leads to apoptosis of gastric epithelial cells.
Assuntos
Apoptose/efeitos dos fármacos , Proteínas de Bactérias/farmacologia , Células Epiteliais/metabolismo , Mucosa Gástrica/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Western Blotting , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Epiteliais/efeitos dos fármacos , Fluoresceína-5-Isotiocianato , Corantes Fluorescentes , Mucosa Gástrica/citologia , Mucosa Gástrica/efeitos dos fármacos , Helicobacter pylori/metabolismo , Humanos , Indóis , Proteínas dos Microfilamentos/biossíntese , Plasmídeos/genética , Proteínas Recombinantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Superóxido Dismutase/biossíntese , Superóxido Dismutase-1 , Vacúolos/efeitos dos fármacos , Proteína X Associada a bcl-2/metabolismoRESUMO
Until now, the multiple biological effects of ionizing radiation on liver have been reported. However, there has not been any reports of fast neutron-mediated liver injuries including liver regeneration or fibrosis. Here, we described the biological effects of acute fast neutron irradiation on the liver. After the fast neutron irradiation of 0, 0.25, 1, 2, 4 and 8 Gy on mice, hepatocyte necrosis and a decrease in the total number of hepatocytes were induced dose-dependently. Binucleated hepatocytes and PCNA positive hepatocytes increased significantly at 0.25 and 1 Gy, but decreased markedly at 2, 4 and 8 Gy. The expression of cytochrome P450 2E1 (CYP2E1) showed a dose-dependent increase after fast neutron irradiation. The activation of p-Smad2/3, signaling intermediates of transforming growth factor-beta (TGF-beta), increased in hepatocytes after exposure of 0.25, 1, and 2 Gy of fast neutrons, but it was not detected in hepatic stellate cells (HSCs). In conclusion, fast neutron-induced liver damages, likely loss of hepatocytes, necrotic foci and vacuolar changes, were note on the dose dependent manner and hepatocellular regeneration were significantly diminished at doses of 2, 4 and 8Gy in a dose-dependent manner. These alterations may at least in part be associated with dose-dependent increase in CYP2E1 and p-Smad2/3. These results show promise as an approach for the treatment of fast neutrons on liver tumors and in the study of pathogenesis regarding the fast neutron-irradiated damages of the liver.
Assuntos
Hepatopatias/patologia , Hepatopatias/fisiopatologia , Fígado/fisiopatologia , Fígado/efeitos da radiação , Nêutrons , Lesões por Radiação/patologia , Lesões por Radiação/fisiopatologia , Animais , Células Cultivadas , Relação Dose-Resposta à Radiação , Hepatócitos/patologia , Hepatócitos/efeitos da radiação , Fígado/patologia , Hepatopatias/etiologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Doses de Radiação , Lesões por Radiação/complicaçõesRESUMO
The wound healing process is a highly orchestrated process, which includes inflammation, re-epithelialization, granulation tissue formation, matrix formation and re-modeling. In this paper, we attempt to determine if bio-active ceramic resource powder particles had an effect on cutaneous wound healing. Furthermore, we investigated its related mechanism and the expression of Smads of cutaneous wound healing, which can be accelerated by bio-active ceramic ointment. Topically applied lesions of 5%, 10% and 15% bio-active ceramic ointment (AO) showed accelerated wound closure, re-epithelialization, and the related immediate down stream of TGF-beta (p-Smad2/3 and Smad3) was suppressed. In particular, 10% and 15% AO lesions became closed faster at Days 3 and 4 of post-wound and p-Smad2/3 was also suppressed. All AO lesions showed accelerated mild wound closure at Day 6, but there were no significant difference. Several papers reported that Smad3 may mediate the signaling pathways that is inhibitory to wound healing, as the deletion of Smad3 leads to enhanced re-epithelialization and contraction of the wound area. This study showed that topical, bio-active ceramic ointment applications accelerated wound closure, re-epithelialization and the suppression of Smad proteins (p-Smad2/3, Smad3). The data revealed that the suppression of Smad3, which was induced by bio-active ceramic resources powder particles affected re-epithelialization and cutaneous wound closure. At the end of this paper, we concluded that bio-active ceramic resources affect cutaneous wound healing by accelerating the re-epithelialization of keratinocytes and that is mediated by the suppression of related protein, Smad3.
Assuntos
Cerâmica/farmacologia , Transdução de Sinais/efeitos dos fármacos , Pele/efeitos dos fármacos , Fator de Crescimento Transformador beta/metabolismo , Cicatrização/efeitos dos fármacos , Animais , Cerâmica/química , Epitélio/efeitos dos fármacos , Imuno-Histoquímica , Masculino , Pomadas/farmacologia , Fosfoproteínas/metabolismo , Ratos , Ratos Sprague-Dawley , Proteína Smad2/metabolismo , Proteína Smad3/metabolismoRESUMO
Glutamate is the major excitatory neurotransmitter in the central nervous system, and evidence for peripheral glutamatergic fibers in mammals is still lacking. However, glutamate receptors have been identified in peripheral organs, including taste buds, myenteric plexus, and pancreatic islet cell. Protection against anoxic damage could also be explained by mechanisms mediated by postsynaptic mGluR2 or mGluR3, such as the inhibition of membrane excitability resulting from a reduction of cAMP formation by a G-protein-dependent modulation of ion channels. In addition, activation of mGluR3 present in glial cells may contribute to neuroprotection by enhancing the production of death. Thus, mGluR2/3 behaves potentially as a major defensive mechanism anoxia-tolerant species. There are a few reports for the regional pattern of hypoxic damage, which was inversely related to the expression of mGluR2/3. The aim of this study was to characterize the expression of mGluR3 in hypoxic liver in experimental model of rat liver. Proteomic analysis of protein extracts from CCl4-induced cirrhotic liver revealed the presence of the mGluR3. The presence of mGluR3 in the cirrhotic liver was confirmed by immunohistochemical analysis. There were a number of macrophages expressing mGluR3 mainly in the fibrous septa. After 2 weeks recovery, however, most of mGluR3 positive macrophages disappeared with collagen fibers. These results demonstrate that mGluR3 involved in the liver in response to persistent hypoxic status such as fibrotic/cirrhotic condition, and suggest that the expression of mGluR3 may be a key role functional metabolism and viability in the liver by interacting with the glutamate receptors in vivo.
Assuntos
Hipóxia/fisiopatologia , Cirrose Hepática Experimental/metabolismo , Receptores de Glutamato Metabotrópico/metabolismo , Regulação para Cima , Animais , Tetracloreto de Carbono/toxicidade , Modelos Animais de Doenças , Fibrose/induzido quimicamente , Fibrose/patologia , Imuno-Histoquímica , Cirrose Hepática Experimental/induzido quimicamente , Cirrose Hepática Experimental/patologia , Masculino , Ratos , Ratos WistarRESUMO
BACKGROUND: The involvement of whether macrophages and mast cells in the kinetics of matrix metalloproteinases-1 (MMP-1), MMP-3 and MMP-9, tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2 in hepatic fibrosis/cirrhosis was examined. MATERIALS AND METHODS: The MMPs and TIMPs were examined by histopathology, immunohistochemistry and immunoblotting using carbon tetrachloride-induced fibrotic rat liver. RESULTS: MMP-1 increased in proportion to the development of fibrosis and reached maximum at week 14. In the first four weeks, MMP-3 expression was mainly observed in many hepatocytes. At week 8, the macrophages in the fibrous septa, as well as hepatocytes, expressed MMP-3. TIMP-1 and -2 progressively increased throughout the experimental periods. The MMP-1 expression in the mast cells, however, did not decrease the degree of liver cirrhosis. CONCLUSION: MMP-1, TIMP-1 and -2 expressions increased in the late stages of fibrosis and cirrhosis. During recovery, the MMP-3 expression of macrophages greatly increased in the unresolved fibrous septa.
Assuntos
Fibrose/enzimologia , Fígado/enzimologia , Macrófagos/metabolismo , Mastócitos/metabolismo , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 3 da Matriz/metabolismo , Animais , Tetracloreto de Carbono/toxicidade , Fibrose/induzido quimicamente , Fibrose/patologia , Immunoblotting , Técnicas Imunoenzimáticas , Cinética , Fígado/patologia , Macrófagos/citologia , Masculino , Ratos , Ratos Wistar , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Inibidor Tecidual de Metaloproteinase-2/metabolismoRESUMO
A male, 5-year-old Jindo dog underwent enterectomy and enteroanastomosis due to ileus of the intestine at a local veterinary hospital. Grossly, the excised intestine showed markedly thickened multinodular masses in the serosal layer of the upper part, and soft-to-firm, cream-colored neoplastic masses that displayed extensive nodular mucosal protuberances into the lumen. The neoplastic masses were filled with large round cells that were ovoid in shape and they had pale and/or hyperchromatic nuclei. The neoplastic cells had mainly infiltrated into the mucosal and submucosal layers, and they had diffusely invaded the muscular and serosal layers. Therefore, the diagnosis of canine multiple intestinal malignant lymphomatous polyposis was made based on the gross and histopathological findings. The origin of these tumor cells was determined to be B-cells since they were positive for anti-CD20.
Assuntos
Doenças do Cão/patologia , Neoplasias Intestinais/veterinária , Pólipos Intestinais/patologia , Linfoma de Célula do Manto/veterinária , Animais , Antígenos CD20/metabolismo , Doenças do Cão/cirurgia , Cães , Imuno-Histoquímica/veterinária , Neoplasias Intestinais/patologia , Neoplasias Intestinais/cirurgia , Pólipos Intestinais/cirurgia , Linfoma de Célula do Manto/patologia , Linfoma de Célula do Manto/cirurgia , MasculinoRESUMO
Three dead dogs were brought to the College of Veterinary Medicine, Kyungpook National University for study. Clinically, all the dogs showed emaciation, anorexia, depression, hemorrhagic vomiting and diarrhea for 7-10 days before death. All the clinical signs were first noted for about one month after feeding the dogs with commercial diets. At necropsy, all 3 dogs had severe renal damage with the same green-yellowish colored nephroliths in the renal pelvis. They also showed systemic hemorrhage and calcification of several organs, which might have been induced by uremia. Microscopically, necrosis, calcification and calculi were detected in the renal tubules, and especially in the proximal convoluted tubules and collecting ducts of the kidney. These findings were supportive of a mycotoxic effect, and especially on their kidneys. However, the precise cause of the toxic effect in these cases of canine renal failure could not be determined.
Assuntos
Injúria Renal Aguda/veterinária , Doenças do Cão/patologia , Micotoxicose/veterinária , Injúria Renal Aguda/microbiologia , Injúria Renal Aguda/patologia , Animais , Doenças do Cão/microbiologia , Cães , Evolução Fatal , Feminino , Histocitoquímica/veterinária , Masculino , Micotoxicose/microbiologia , Micotoxicose/patologiaRESUMO
The objective of this study was to examine alcohol-induced changes of bone in hormone-deficient males using the developed method. In the process of bone resorption, type I collagen crosslinking molecules, pyridinoline (PYD), are released into the circulation and cleared by the kidneys. (2)H(2)O as a tracer has been applied to measure the synthesis rates of slow-turnover proteins and successfully applied to bone collagen synthesis in our hormone deficiency rats. This study demonstrated for the first time, the early changes of the femur bone degradation in hormone-deficient male individuals, more influenced by alcohol through histopathological study, serum PYD assay, and (2)H(2)O labeling. We also observed that serum PYD was a sensitive pathological marker of bone degradation in castrated osteoporosis males and the unique features of (2)H(2)O labeling to measure the bone turnover collagen synthesis rates were excellent markers of bone degradation and aging.
Assuntos
Reabsorção Óssea/patologia , Etanol/toxicidade , Fêmur/patologia , Aminoácidos/metabolismo , Animais , Reabsorção Óssea/induzido quimicamente , Reabsorção Óssea/metabolismo , Colágeno Tipo I/metabolismo , Deutério , Etanol/administração & dosagem , Fêmur/efeitos dos fármacos , Fêmur/metabolismo , Masculino , Orquiectomia , Ratos , Ratos Wistar , ÁguaRESUMO
BACKGROUND: The aim of this study was to determine the induction and distribution of Mallory body (MB) and oval cells in carbon-tetrachloride (CCl4)-induced rat liver fibrosis. MATERIALS AND METHODS: MBs and oval cells expressing cytokeratins (CKs) 8 and 18 were monitored by immuno-histochemistry and immunoblotting. RESULTS: MBs were mainly detected within hepatocytes near the fibrotic areas, and oval cells were located along or in the fibrotic areas. Both MBs and oval cells increased in size and number in the development of fibrosis. At cirrhotic liver, most of the oval cells were located in the fibrous septa and around newly formed bile ductules. Moreover, as hepatic injuries developed into fibrosis, a much more prominent single band of CK18 was detected. CONCLUSION: The occurrence and distribution of MB and oval cells in CCl4-induced rat liver fibrosis are reported. This represents the first CCl4 experimental in vivo model of MB induction, which will be useful for further investigations on the pathogenesis of MB.
Assuntos
Hepatócitos/metabolismo , Corpos de Inclusão/metabolismo , Queratinas/metabolismo , Cirrose Hepática Experimental/metabolismo , Animais , Tetracloreto de Carbono , Hepatócitos/patologia , Técnicas Imunoenzimáticas , Corpos de Inclusão/patologia , Fígado/efeitos dos fármacos , Fígado/metabolismo , Fígado/patologia , Cirrose Hepática Experimental/etiologia , Cirrose Hepática Experimental/patologia , Masculino , Ratos , Ratos WistarRESUMO
Among many detrimental injuries, alcohol is implicated in hepatitis, fatty liver, hepatic fibrosis, and cirrhosis. The purpose of this study was to evaluate the protective effect of bio-active ceramic water on alcohol-induced hepatic injury in pigs. Twelve male Landrace pigs were divided into 3 groups. Groups 1, 2, and 3 were fed with bio-active ceramic water + normal liquid diet, bio-active ceramic water + liquid diet containing 15% ethanol, and tap water + liquid diet containing 15% ethanol for 12 weeks, respectively. For serological, histopathological, and immunohistochemical analysis, all pigs were sacrificed at week 12. In group 3, serum ALT and AST levels increased, and mild fatty change and moderate necrosis were detected in the liver. Collagen fibers, myofibroblasts, and CYP2E1 were also increased or activated in group 3. In group 2, there were mild hepatic injuries compared to group 3. However, injuries and activations were not observed in the liver in group 1. We suggest that the bio-active ceramic water used in the present study had protective capability against ethanol-induced hepatic injury and that having no toxic effect on the pig liver. The bio-active ceramic water might be useful as a therapeutic drinking water in patients suffering from alcoholic liver diseases.
Assuntos
Hepatopatias Alcoólicas/dietoterapia , Hepatopatias Alcoólicas/patologia , Água/administração & dosagem , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Cerâmica , Colesterol/sangue , Colágeno , Citocromo P-450 CYP2E1/metabolismo , Imuno-Histoquímica , Albumina Sérica , Sus scrofa , Purificação da Água/métodosRESUMO
BACKGROUND: To find a non-invasive method of diagnosing hepatic fibrosis and cirrhosis, we evaluated the relationship of the hepatic cirrhosis grade between histopathology and mean grey level (MGL) in B-mode ultrasonography in CCl4-induced liver cirrhosis. MATERIALS AND METHODS: Three groups of rats were treated with olive oil, CCl4, and CCl4 + silymarin. Rats were sacrificed at weeks 4, 8 and 12, after B-mode ultrasonography examination, and then analyzed histopathologically for fatty change and fibrosis. RESULTS: On the grade of fibrosis, the CCl4 group showed higher value at 8 and 12 weeks than the silymarin group. However, the fatty change was enhanced in the silymarin group, compared with the CCl4 group. The B-mode histogram values were the highest in the silymarin group, but the collagen rate was highest in the CCl4-treated group, at week 12. These results suggest that the B-mode histogram can be more affected by infiltration of lipid than by intact accumulation of collagen fibers. CONCLUSION: In the histogram of 8 and 12 weeks, there were significant differences between the CCl4-treated group and silymarin group in mean grey levels of B-mode ultrasonography. The histogram of B-mode mean grey level has a close correlation with fatty change and is useful for the diagnosis of liver fibrosis by histopathological analysis.