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Atom-dispersed low-coordinated transition metal-Nx catalysts exhibit excellent efficiency in activating peroxydisulfate (PDS) for environmental remediation. However, their catalytic performance is limited due to metal-N coordination number and single-atom loading amount. In this study, low-coordinated nitrogen-doped graphene oxide (GO) confined single-atom Mn catalyst (Mn-SA/NGO) was synthesized by molten salt-assisted pyrolysis and coupled to PDS for degradation of tetracycline (TC) in water. Aberration-corrected high-angle annular dark-field scanning transmission electron microscopy (AC-HAADF-STEM) and X-ray absorption fine structure spectroscopy (XAFS) analysis showed the successful doping of single-atom Mn (weight percentage 1.6%) onto GO and the formation of low-coordinated Mn-N2 sites. The optimized parameters obtained by Box-Behnken Design achieved 100% TC removal in both prediction and experimental results. The Mn-SA/NGO + PDS system had strong anti-interference ability for TC removal in the presence of anions. Besides, Mn-SA/NGO possessed good reusability and stability. O2â¢-, â¢OH, and 1O2 were the main active species for TC degradation, and the TC mineralization reached 85.1%. Density functional theory (DFT) calculations confirmed that the introduction of single atoms Mn could effectively enhance adsorption and activation of PDS. The findings provide a reference for the synthesis of high-performance single-atom catalysts for effective removal of antibiotics.
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Introduction: To analyze the influencing factors for progression from newly diagnosed prediabetes (PreDM) to diabetes within 3 years and establish a prediction model to assess the 3-year risk of developing diabetes in patients with PreDM. Methods: Subjects who were diagnosed with new-onset PreDM at the Physical Examination Center of the First Affiliated Hospital of Soochow University from October 1, 2015 to May 31, 2023 and completed the 3-year follow-up were selected as the study population. Data on gender, age, body mass index (BMI), waist circumference, etc. were collected. After 3 years of follow-up, subjects were divided into a diabetes group and a non-diabetes group. Baseline data between the two groups were compared. A prediction model based on logistic regression was established with nomogram drawn. The calibration was also depicted. Results: Comparison between diabetes group and non-diabetes group: Differences in 24 indicators including gender, age, history of hypertension, fatty liver, BMI, waist circumference, systolic blood pressure, diastolic blood pressure, fasting blood glucose, HbA1c, etc. were statistically significant between the two groups (P<0.05). Differences in smoking, creatinine and platelet count were not statistically significant between the two groups (P>0.05). Logistic regression analysis showed that ageing, elevated BMI, male gender, high fasting blood glucose, increased LDL-C, fatty liver, liver dysfunction were risk factors for progression from PreDM to diabetes within 3 years (P<0.05), while HDL-C was a protective factor (P<0.05). The derived formula was: In(p/1-p)=0.181×age (40-54 years old)/0.973×age (55-74 years old)/1.868×age (≥75 years old)-0.192×gender (male)+0.151×blood glucose-0.538×BMI (24-28)-0.538×BMI (≥28)-0.109×HDL-C+0.021×LDL-C+0.365×fatty liver (yes)+0.444×liver dysfunction (yes)-10.038. The AUC of the model for predicting progression from PreDM to diabetes within 3 years was 0.787, indicating good predictive ability of the model. Conclusions: The risk prediction model for developing diabetes within 3 years in patients with PreDM constructed based on 8 influencing factors including age, BMI, gender, fasting blood glucose, LDL-C, HDL-C, fatty liver and liver dysfunction showed good discrimination and calibration.
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Estado Pré-Diabético , Humanos , Estado Pré-Diabético/epidemiologia , Estado Pré-Diabético/sangue , Estado Pré-Diabético/complicações , Masculino , Feminino , Pessoa de Meia-Idade , Fatores de Risco , Adulto , Progressão da Doença , Seguimentos , Medição de Risco , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/epidemiologia , Índice de Massa Corporal , Glicemia/análise , Glicemia/metabolismo , Idoso , Circunferência da Cintura , Prognóstico , China/epidemiologiaRESUMO
COVID-19 caused by Omicron BA.1 has resulted in a global humanitarian crisis. In this COVID-19 pandemic era, hypertension has been receiving increased attention. Omicron BA.1 infection combined with hypertension created a serious public health problem and complicated the treatment and prognosis of COVID-19. The aim of our study was to assess the implications of hypertension for the clinical manifestations of adult patients (APs) infected with Omicron BA.1. This single-center retrospective cohort study enrolled consecutive COVID-19 APs, who were admitted to Tianjin First Central Hospital from 01 August 2022 to 30 November 2022. All included APs were divided into two groups: hypertension and non-hypertension group. The APs' baseline demographic, laboratory, clinical, and radiological characteristics were collected and analyzed. Of 512 APs admitted with PCR proven COVID-19, 161 (31.45%) APs had comorbid hypertension. Hypertension APs have older age, higher body mass index, lower Ct-values of the viral target genes at admission, and longer hospital stay than non-hypertension APs. Furthermore, hypertension aggravates the clinical classification, impairs liver, kidney, and myocardium function, and abnormalizes the coagulation system in Omicron BA.1- infected APs. Moreover, hypertension elevates inflammation levels and lung lesion involvement while weakened virus-specific IgM level in APs with Omicron BA.1 infection. Hypertension APs tend to have worse clinical conditions at baseline than those non-hypertension APs. This study indicates that hypertension is a contributor to the poor clinical manifestations of Omicron BA.1-infected APs and supports that steps to control blood pressure should be a vital consideration for reducing the burden of Omicron BA.1 infection in hypertension individuals. IMPORTANCE: This study provided inclusive insight regarding the relationship between hypertension and Omicron BA.1 infection and supported that hypertension was an adverse factor for COVID-19 APs. In conclusion, this study showed that hypertension was considered to be associated with severe conditions, and a contributor to poor clinical manifestations. Proper medical management of hypertension patients is an imperative step in mitigating the severity of Omicron BA.1 variant infection.
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COVID-19 , Hipertensão , SARS-CoV-2 , Humanos , COVID-19/complicações , COVID-19/virologia , Hipertensão/complicações , Masculino , Feminino , Pessoa de Meia-Idade , SARS-CoV-2/genética , Estudos Retrospectivos , Adulto , IdosoRESUMO
BACKGROUND: Atherosclerosis (AS) is the leading cause of global death, which manifests as arterial lipid stack and plaque formation. Geniposide is an iridoid glycoside extract from Gardenia jasminoides J.Ellis that ameliorates AS by mediating autophagy. However, how Geniposide regulates autophagy and treats AS remains unclear. PURPOSE: To evaluate the efficacy and mechanism of Geniposide in treating AS. STUDY DESIGN AND METHODS: Geniposide was administered to high-fat diet-fed ApoE-/- mice and oxidized low-density lipoprotein-incubated primary vascular smooth muscle cells (VSMCs). AS was evaluated with arterial lipid stack, plaque progression, and collagen loss in the artery. Foam cell formation was detected by lipid accumulation, inflammation, apoptosis, and the expression of foam cell markers. The mechanism of Geniposide in treating AS was assessed using network pharmacology. Lipophagy was measured by lysosomal activity, expression of lipophagy markers, and the co-localization of lipids and lipophagy markers. The effects of lipophagy were blocked using Chloroquine. The role of PARP1 was assessed by Olaparib (a PARP1 inhibitor) intervention and PARP1 overexpression. RESULTS: In vivo, Geniposide reversed high-fat diet-induced hyperlipidemia, plaque progression, and inflammation. In vitro, Geniposide inhibited VSMC-derived foam cell formation by suppressing lipid stack, apoptosis, and the expressions of foam cell markers. Network pharmacological analysis and in vitro validation suggested that Geniposide treated AS by enhancing lipophagy via suppressing the PI3K/AKT signaling pathway. The benefits of Geniposide in alleviating AS were offset by Chloroquine in vivo and in vitro. Inhibiting PARP1 using Olaparib promoted lipophagy and alleviated AS progression, while PARP1 overexpression exacerbated foam cell formation and lipophagy blockage. The above effects of PARP1 were weakened by PI3K inhibitor LY294002. PARP1 also inhibited the combination of the ABCG1 and PLIN1. CONCLUSION: Geniposide alleviated AS by restoring PARP1/PI3K/AKT signaling pathway-suppressed lipophagy. This study is the first to present the lipophagy-inducing effect of Geniposide and the binding of ABCG1 and PLIN1 inhibited by PARP1.
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Aterosclerose , Dieta Hiperlipídica , Iridoides , Fosfatidilinositol 3-Quinases , Poli(ADP-Ribose) Polimerase-1 , Proteínas Proto-Oncogênicas c-akt , Transdução de Sinais , Animais , Iridoides/farmacologia , Aterosclerose/tratamento farmacológico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fosfatidilinositol 3-Quinases/metabolismo , Poli(ADP-Ribose) Polimerase-1/metabolismo , Masculino , Camundongos , Dieta Hiperlipídica/efeitos adversos , Autofagia/efeitos dos fármacos , Gardenia/química , Músculo Liso Vascular/efeitos dos fármacos , Camundongos Endogâmicos C57BL , Células Espumosas/efeitos dos fármacos , Células Espumosas/metabolismo , Miócitos de Músculo Liso/efeitos dos fármacos , Miócitos de Músculo Liso/metabolismo , Farmacologia em Rede , Lipoproteínas LDLRESUMO
Objective: To investigate the impact of critical care management on patients diagnosed with acute respiratory distress syndrome (ARDS) during their stay in intensive care units (ICU). Methods: A total of 62 ARDS patients admitted to the ICU of our hospital between March 2020 and March 2023 were enrolled as research participants. The patients were randomly assigned to either the research group (n=31) receiving critical care management or the control group (n=31) receiving routine care. Key parameters, including the duration of ICU stay, mean hospital length of stay (HLOS), duration of mechanical ventilation, and the incidence of ventilator-associated pneumonia (VAP), were recorded. Additionally, changes in the Self-rating Anxiety/Depression Scale (SAS/SDS), Pittsburgh Sleep Quality Index (PSQI), Acute Physiology and Chronic Health Evaluation II (APACHE), and Clinical Pulmonary Infection Score (CPIS) were assessed before and after the intervention. A self-designed questionnaire was employed to evaluate nursing quality and patient satisfaction. Results: In comparison to the control group, the research group exhibited significantly shorter durations of ICU stay, mean HLOS, and mechanical ventilation, along with a reduced incidence of VAP (P < .05). Moreover, the SAS, SDS, PSQI, APACHE II, and CPIS scores in the research group were lower than those in the control group, while the quality of care and satisfaction scores were notably higher (P < .05). Conclusions: ICU critical care management demonstrates the potential to enhance the rehabilitation of ARDS patients and decrease the incidence of VAP, suggesting its clinical applicability.
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Unidades de Terapia Intensiva , Tempo de Internação , Síndrome do Desconforto Respiratório , Humanos , Síndrome do Desconforto Respiratório/terapia , Masculino , Feminino , Pessoa de Meia-Idade , Unidades de Terapia Intensiva/estatística & dados numéricos , Tempo de Internação/estatística & dados numéricos , Adulto , Idoso , Cuidados Críticos/métodos , Respiração Artificial , APACHERESUMO
Aim: To assess baseline histogram parameters from apparent diffusion coefficient (ADC) images in predicting early treatment response in newly diagnosed multiple myeloma (NDMM) patients. Methods: The histogram parameters of lesions in 68 NDMM patients were obtained with the Firevoxel software. The presence of deep response after two cycles of induction was recorded. Results: Some parameters were significantly different between the two groups, for example, ADC 75% in lumbar spine (p = 0.026). No significant difference in mean ADC for any anatomic site was found (all p > 0.05). The combination of ADC 75, ADC 90 and ADC 95% in lumbar spine; ADC skewness and ADC kurtosis in rib achieved a sensitivity of 100% in predicting deep response. Conclusion: Histogram analysis of ADC images can describe NDMM heterogeneity and accurately predict treatment response.
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Mieloma Múltiplo , Humanos , Mieloma Múltiplo/diagnóstico por imagem , Mieloma Múltiplo/terapia , Interpretação de Imagem Assistida por Computador/métodos , Imagem de Difusão por Ressonância Magnética/métodos , Imageamento por Ressonância Magnética , Software , Estudos RetrospectivosRESUMO
The frequent occurrence of algal blooms in water bodies leads to a significant accumulation of microcystin-LR (MC-LR). In this study, we developed a porous foam-like self-floating N-deficient g-C3N4 (SFGN) photocatalyst for efficient photocatalytic degradation of MC-LR. Both the characterization results and DFT calculations indicate that the surface defects and floating state of SFGN synergistically enhance light harvesting and photogenerated carrier migration rate. The photocatalytic process achieved a nearly 100 % removal rate of MC-LR within 90 min, while the self-floating state of SFGN maintained good mechanical strength. ESR and radical capture experiments revealed that the primary active species responsible for the photocatalytic process was OH. This finding confirmed that the fragmentation of MC-LR occurs as a result of OH attacking the MC-LR ring. LC-MS analysis indicated that majority of the MC-LR molecules were mineralized into small molecules, allowing us to infer possible degradation pathways. Furthermore, after four consecutive cycles, SFGN exhibited remarkable reusability and stability, highlighting the potential of floating photocatalysis as a promising technique for MC-LR degradation.
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Luz , Microcistinas , Fotólise , CatáliseRESUMO
Serotonin modulates many motivated behaviours via multiple receptor subtypes. Agonists at 5-HT2C receptors have potential for treating behavioural problems associated with obesity and drug use. In this work we examined the impact of the 5-HT2C receptor agonist lorcaserin on several motivated behaviours related to feeding, reward and waiting impulsivity, and on neuronal activation in key brain areas mediating those behaviours. In male C57BL/6J mice effects of lorcaserin (0.2, 1 and 5 mg/kg) were examined on feeding, and on operant responding for a palatable reward. Feeding was reduced only at 5 mg/kg, whereas operant responding was reduced at 1 mg/kg. At a much lower dose range lorcaserin 0.05-0.2 mg/kg also reduced impulsive behaviour measured as premature responding in the 5-choice serial reaction time (5-CSRT) test, without affecting attention or ability to perform the task. Lorcaserin induced Fos expression in brain regions related to feeding (paraventricular nucleus and arcuate nucleus), reward (ventral tegmental area), and impulsivity (medial prefrontal cortex, VTA) although these effects did not show the same differential sensitivity to lorcaserin as the behavioural measures. These results indicate a broad profile of action of 5-HT2C receptor stimulation on brain circuitry and on motivated behaviours, but with clear evidence of differential sensitivity across behavioural domains. This is exemplified by the fact that impulsive behaviour was reduced at a much lower dose range than was feeding behaviour. Along with previous work, and some clinical observations, this work supports the idea that 5-HT2C agonists may be useful for behavioural problems associated with impulsivity.
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Receptor 5-HT2C de Serotonina , Serotonina , Animais , Masculino , Camundongos , Comportamento Impulsivo , Camundongos Endogâmicos C57BL , Recompensa , Serotonina/farmacologia , Agonistas do Receptor 5-HT2 de Serotonina/farmacologiaRESUMO
The development of novel catalysts for degrading organic contaminants in water is a current hot topic in photocatalysis research for environmental protection. In this study, C3N5 nanosheet/Ag2CO3 nanocomposites (CNAC-X) were used as efficient photocatalysts for the visible-light-driven degradation of methylene blue (MB), and tetracycline hydrochloride (TC-HCl) was synthesized for the first time using a simple thermal oxidative exfoliation and in situ deposition method. Due to the synergistic effect of nanosheet structures, carbon defects, and Z-scheme heterojunctions, CNAC-10 exhibited the highest photocatalytic activity, with photodegradation efficiencies of 96.5% and 97.6% for MB (60 mg/L) and TC-HCl (50 mg/L) within 90 and 100 min, respectively. The radical trapping experiments showed that ·O2- and h+ played major roles in the photocatalytic effect of the CNAC-10 system. Furthermore, intermediates in the photodegradation of MB and TC-HCl were investigated to determine possible mineralization pathways. The results indicated that C3N5 nanosheet/Ag2CO3 photocatalysts prepared in this work could provide an effective reference for the treatment of organic wastewater.
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Slopes along the highway and railway routes are subjected to not only static loads but also dynamic loads generated by vehicles and trains. The induced excessive deformation potentially poses a threat to slope stability. In terms of the extensive application of ecological slope protection, plants play a critical role in slope stability, as the roots can enhance the shear strength of the soil. This study aims to investigate the influence of different root distribution patterns on the dynamic characteristics induced by cyclic loading. By conducting a group of dynamic triaxial tests, the results indicate that the root system can significantly enhance the liquefaction resistance of the soil when the soil is subjected to lower dynamic loads, and the cross arrangement has a better-reinforced effect than the mixed arrangement. The reinforced effect was not obvious when the soil was subjected to a dynamic load with a larger stress amplitude. In addition, based on the validation of the seed model, a new pore water pressure development model was proposed according to the test results. Overall, the research provides a new model and some innovative observations to better understand the dynamic behavior of root-reinforced soil.
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Plantas , Solo , Resistência ao CisalhamentoRESUMO
As an ecological green building material, natural bamboo has many advantages such as a light weight, high strength, and short growth cycle. Natural bamboo is widely used in landscape architecture and fabricated structures. However, in bamboo building structures, the most common bolted joints often appear cleaved along the grain. In this paper, glass fiber-reinforced polymer (GFRP) is designed to wrap and improve the shear capacity of natural bamboo-bolt composite joints. According to the corresponding material parameters, the finite element model of composite joints is established, and the key influencing variables of the bearing capacity, namely the bolt diameter, bamboo tube outer diameter, and screw end distance, are analyzed. In addition, according to the European analytical yield model of bolted connections, the analytical calculation method of the bearing capacity is proposed and compared with the experimental and simulated values. The results showed that the numerical model and the modified analytical model can suitably describe the bearing capacity of composite joints, and a higher bolt diameter, along with the bamboo outer diameter, will lead to a higher ultimate load of the composite joints. Moreover, the bearing capacity of composite joints has no obvious relationship with the end spacing.
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Vegetation plays a vital role in regulating hydrological cycle and controlling soil erosion at multiple spatial and temporal scales. Establishing shrub-grass community is one of the widely adopted practices to increase rainfall infiltration and reduce soil erosion in water-limited and highland regions. To understand the effects of such vegetation communities on soil erosion and overland flow under different rainfall regimes at the hillslope scale, we conducted rainfall simulation experiments by setting up parallel plots at fixed slope of 15° including unvegetated (coverage 0%), shrub only (coverage 50%), grass only (coverage 50%), and shrub-grass covered (coverages 25%, 50%, 75%, and 100%) and constant rainfall intensities of 30, 60, and 90 mm h-1 rainfalls lasting 60 min each after the initiation of overland flow. Two native species Lespedeza bicolor and Carex giraldiana, distributed in the soil sampling region were planted on the plots to achieve designed coverages. We found that the overland flow and sediment load from vegetated slopes were reduced by 9%-58% and 27%-98%, respectively, compared with unvegetated slopes while the infiltration rate increased by over 45%. Shrub-grass community reduced the overland flow and sediment yield more significantly than shrub only and grass only treatments with the same coverage of 50% under three rainfall intensities. In addition, the overland flow rate linearly decreased while the mean sediment yield exponentially reduced against the increase in shrub-grass community coverage. Hydrodynamically, shrub-grass communities not only increased the critical hydrodynamic forces for the initiating soil erosion but also increased the resistance coefficient leading to reduce overland flow velocity, stream power, and thus soil erosion from the vegetative slope even under extreme rainfalls. Our research highlights the importance of developing the shrub-grass communities to reduce the quantity and energy of overland flow and control soil erosion on the hillslopes in water-limited and highland regions.
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Poaceae , Erosão do Solo , China , Monitoramento Ambiental , Chuva , Solo , Água , Movimentos da ÁguaRESUMO
The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) modulates fundamental motivational processes, and the neurochemical and behavioural effects of drugs of abuse. Recently, attention has focused on the role of 5-HT acting via 5-HT2A and 5-HT2C receptor sub-types in this regard. We examined the impact of manipulating 5-HT2A and 5-HT2C receptor mediated function on several aspects of alcohol self-administration and alcohol-seeking behaviour in male and female rats. Specifically, experiments investigated the effect of the 5-HT2A inverse agonist/antagonist pimavanserin, and the 5-HT2C receptor agonist lorcaserin on these behaviours. In male and female rats trained to respond for alcohol reinforcement on fixed ratio (FR) and progressive ratio (PR) schedules of reinforcement pimavanserin (0.3, 1 and 3 mg/kg) had no consistent effect on responding. Lorcaserin (0.25, 0.5 and 1 mg/kg) reduced these behaviours in both sexes. Following extinction of responding for alcohol, alcohol-seeking was reinstated by cues previously paired with alcohol. Pimavanserin (1 mg/kg) and lorcaserin (0.5 mg/kg) significantly reduced this reinstatement. In a two-bottle 24 h intermittent access procedure pimavanserin had no significant effects, but lorcaserin reduced alcohol consumption in both sexes at 1, 4 and 24 h after access to alcohol was allowed. Finally, as determined using in vivo microdialysis, alcohol increased, and lorcaserin (0.5 mg/kg) reduced, extracellular levels of DA in the NAc in male rats. In rats treated with lorcaserin prior to alcohol injection the net effect was that DA levels were not changed compared to those measured in control rats. These results suggest that blocking 5-HT2A receptor activity has a very limited action to reduce alcohol-seeking. Activating 5-HT2C receptors had a broader behavioural profile to reduce alcohol self-administration, alcohol drinking and alcohol seeking. These effects may partly result from a blunting of the effect of alcohol on mesolimbic DA release.
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Agonistas do Receptor 5-HT2 de Serotonina , Serotonina , Animais , Benzazepinas , Relação Dose-Resposta a Droga , Feminino , Masculino , Piperidinas , Ratos , Receptor 5-HT2C de Serotonina , Autoadministração , Agonistas do Receptor 5-HT2 de Serotonina/farmacologia , Ureia/análogos & derivadosRESUMO
Aim: This study aimed to predict progression-free survival (PFS) in patients with early glottic cancer using radiomic features on dual-energy computed tomography iodine maps. Methods: Radiomic features were extracted from arterial and venous phase iodine maps, and radiomic risk scores were determined by univariate Cox proportional hazards regression analysis and least absolute shrinkage and selection operator regression with tenfold cross-validation. The Kaplan-Meier method was used to evaluate the association between radiomic risk scores and PFS. Results: Patients were stratified into low-risk and high-risk groups using radiomics, the PFS corresponding rates with statistical significance between the two groups. The high-risk group showed better survival, benefiting from laryngectomy. Conclusion: Radiomics could provide a promising biomarker for predicting the PFS of early glottic cancer patients.
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Iodo , Neoplasias Laríngeas , Humanos , Neoplasias Laríngeas/diagnóstico por imagem , Prognóstico , Intervalo Livre de Progressão , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodosRESUMO
OBJECTIVES: To establish and validate a predictive model integrating with clinical and dual-energy CT (DECT) variables for individual recurrence-free survival (RFS) prediction in early-stage glottic laryngeal cancer (EGLC) after larynx-preserving surgery. METHODS: This retrospective study included 212 consecutive patients with EGLC who underwent DECT before larynx-preserving surgery between January 2015 and December 2018. Using Cox proportional hazard regression model to determine independent predictors for RFS and presented on a nomogram. The model's performance was assessed using Harrell's concordance index (C-index), time-dependent area under curve (TD-AUC) plot, and calibration curve. A risk stratification system was established using the nomogram with median scores of all cases to divide all patients into two prognostic groups. RESULTS: Recurrence occurred in 39/212 (18.4%) cases. Normalized iodine concentration in arterial (NICAP) and venous phases (NICVP) were verified as significant predictors of RFS in multivariate Cox regression (hazard ratio [HR], 4.2; 95% confidence interval [CI]: 2.3, 7.7, p < .001 and HR, 3.0; 95% CI: 1.5, 5.9, p = .002, respectively). Nomogram based on clinical and DECT variables was better than did only clinical variables. The prediction model proved well-calibrated and had good discriminative ability in the training and validation samples. A risk stratification system was built that could effectively classify EGLC patients into two risk groups. CONCLUSIONS: DECT could provide independent RFS indicators in patients with EGLC, and the nomogram based on DECT and clinical variables was useful in predicting RFS at several time points. KEY POINTS: ⢠Dual-energy CT(DECT) variables can predict recurrence-free survival (RFS) after larynx-preserving surgery in patients with early-stage glottic laryngeal cancer (EGLC). ⢠The model that integrates clinical and DECT variables predicted RFS better than did only clinical variables. ⢠A risk stratification system based on the nomogram could effectively classify EGLC patients into two risk groups.
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Neoplasias Laríngeas , Laringe , Humanos , Neoplasias Laríngeas/diagnóstico por imagem , Neoplasias Laríngeas/cirurgia , Recidiva Local de Neoplasia/diagnóstico por imagem , Recidiva Local de Neoplasia/patologia , Estadiamento de Neoplasias , Nomogramas , Prognóstico , Estudos Retrospectivos , Medição de Risco , Tomografia Computadorizada por Raios XRESUMO
BACKGROUND. The recently released Myeloma Response Assessment and Diagnosis System (MY-RADS) for multiple myeloma (MM) evaluation using whole-body MRI (WB-MRI) describes the total burden score. However, assessment is confounded by red bone marrow hyperplasia in anemia. OBJECTIVE. The purpose of this study is to assess the utility of the MY-RADS total burden score, ADC, and fat fraction (FF) from WB-MRI in predicting early treatment response in patients with newly diagnosed MM and to compare the utility of these measures between patients with and without anemia. METHODS. This retrospective study included 56 patients (40 men, 16 women; mean age, 57.4 ± 9.6 [SD] years) with newly diagnosed MM who underwent baseline WB-MRI including DWI and modified Dixon sequences. Two radiologists recorded total burden score using MY-RADS and measured the ADC and FF of diffuse and focal disease sites. Mean values across sites were derived. Interobserver agreement was evaluated, and the mean assessments of the readers were used for further analyses. Presence of deep response after four cycles of induction chemotherapy was recorded. Patients were classified as having anemia if their hemoglobin level was less than 100 g/L. The utility of WBMRI parameters in predicting deep response was assessed. RESULTS. A total of 24 of 56 patients showed deep response, and 25 of 56 patients had anemia. Interobserver agreement, which was expressed using intraclass correlation coefficients, ranged from 0.95 to 0.99. Among patients without anemia, those with deep response compared with those without deep response had a lower total burden score (9.0 vs 18.0), a lower ADC (0.79 × 10-3 mm2/s vs 1.08 × 10-3 mm2/s), and a higher FF (0.21 vs 0.10) (all p < .001). The combination of these three parameters (optimal cutoffs: ≤ 15 for total burden score, ≤ 0.84 × 10-3 mm2/s for ADC, and > 0.16 for FF) achieved sensitivity of 93.8%, specificity of 93.3%, and accuracy of 93.5% for predicting deep response. In patients with anemia, none of the three parameters were significantly different between patients with and without deep response (all p > .05), and the combination of parameters achieved sensitivity of 56.3%, specificity of 100.0%, and accuracy of 72.0%. CONCLUSION. Low total burden score, low ADC, and high FF from WB-MRI may predict deep response in patients with MM, although only among those without anemia. CLINICAL IMPACT. WB-MRI findings may help guide determination of prognosis and initial treatment selection in MM.
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Anemia/complicações , Imageamento por Ressonância Magnética/métodos , Mieloma Múltiplo/diagnóstico por imagem , Mieloma Múltiplo/patologia , Sistemas de Informação em Radiologia , Imagem Corporal Total/métodos , Tecido Adiposo/diagnóstico por imagem , Efeitos Psicossociais da Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mieloma Múltiplo/complicações , Valor Preditivo dos Testes , Estudos Retrospectivos , Resultado do TratamentoRESUMO
The development of steel in engineering structures or components faces the problems of high cost and high carbon emission, which demands new materials used as reinforcement to be proposed and applied. Bamboo is a green and renewable natural material, and has higher tensile strength parallel to grain compared to wood. Based on the excellent mechanical properties in the parallel-to-grain direction of bamboo fiber, this paper proposed an innovative bamboo fiber-reinforced polymer tendon with U-head (BFRP tendon). To analyze the tension capacity and mechanical behavior of the BFRP tendon, the thin-shell model and thick-shell model have been proposed in succession. Both models were compared with the results of tensile test and good agreement was achieved in the stress distribution and the ultimate load. The tension capacity of the BFRP tendon has been proved to be reliable, which can give full play to the advantages of bamboo fiber.
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BACKGROUND: To study the incidence of metabolic syndrome (MetS) and kidney stones in a healthy screening population and to explore the correlation between them. METHODS: The physical examination data of 11,827 people screened at the First Affiliated Hospital of Soochow University from August 2019 to July 2020 were analyzed. MetS diagnostic criteria were based on the 2004 guidelines of Chinese Diabetes Society (CDS). Multivariate logistic regression was used to analyze the correlation between MetS and various characteristics and kidney stones. Trend analysis was represented by P value, and P<0.05 indicated statistical significance. RESULTS: The present study comprised 6,570 males (55.6%, aged 46.15±13.653 years) and 5,257 females (44.4%, aged 41.41±11.712 years). Of these, 1,036 (8.8%) had kidney stones and 1,552 (13.1%) had MetS. Among the MetS patients, 35.1% had a body mass index (BMI) ≥25, 27.7% had hypertension, 10.8% had hyperglycemia, and 31.2% had dyslipidemia. Kidney stone morbidity was 14.5% in the MetS group and 7.9% in the non-MetS group (P<0.05). As the number of MetS characteristics increased, kidney stone morbidity showed a linear increasing trend (P<0.05 for trend). With an increase in BMI and blood triglycerides (TG), and a decrease in lipoprotein cholesterol (HDL-C), the incidence of kidney stones had an increasing trend (P<0.05 for trend). Sex, age and MetS were independent risk factors for the occurrence of kidney stones, with and odds ratio (OR) of 1.493 [95% confidence interval (CI): 1.264-1.763] for MetS. Of the MetS characteristics, BMI ≥25 and blood pressure (BP) ≥140/90 mmHg were independent risk factors for kidney stones, with OR values of 1.209 (95% CI: 1.047-1.396) and 1.248 (95% CI: 1.071-1.453), respectively. CONCLUSIONS: MetS is an independent risk factor for kidney stones. Appropriate medication and dietary advice may help to correct urinary metabolic abnormalities and prevent the recurrence of kidney stones.
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The multiplexed cancer cell line screening platform PRISM demonstrated its utility in testing hundreds of cell lines in a single run, possessing the potential to speed up anti-cancer drug discovery, validation and optimization. Here we described the development and implementation of a next-generation PRISM platform combining Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-mediated gene editing, cell line DNA barcoding and next-generation sequencing to enable genetic and/or pharmacological assessment of target addiction in hundreds of cell lines simultaneously. Both compound and CRISPR-knockout PRISM screens well recapitulated the results from individual assays and showed high consistency with a public database.