Circ_0008768 Suppresses the Pancreatic Cancer Progression via miR-330- 3p/PTEN Axis.

OBJECTIVE: To probe the role of circular RNA_0008768 (circ_0008768) in the development of pancreatic cancer (PC) and its regulatory mechanism. METHODS: The expression levels of circ_0008768, miR-330-3p, and PTEN mRNA in PC tissues and cells were detected using qRT-PCR. Cell proliferation, migration and invasion of PC cells were detected by CCK-8 method, EdU method, and Transwell assay. The targeting relationship between circ_0008768 and miR-330-3p, as well as miR-330-3p and PTEN mRNA 3'UTR was analyzed by the dual-luciferase reporter assay. PTEN expression levels in PC cells were analyzed by Western blot. RESULTS: The expression levels of circ_0008768 and PTEN mRNA were significantly reduced in both PC tissues and cell lines. Overexpression of circ_0008768 exerted an inhibitory effect on the proliferation, migration and invasion of PC cells. Knocking down circ_0008768 showed the opposite effect. Circ_0008768 directly targeted and negatively regulated the expression of miR-330- 3p. PTEN was identified as a downstream target gene of miR-330-3p. Circ_0008768 could positively regulate the expression of PTEN. CONCLUSION: In PC, circ_0008768 can act as a tumor-suppressive factor to inhibit the development of PC by regulating the miR-330-3p/PTEN molecular axis.

FGD5­AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/ß­catenin signaling pathway via miR­577.

The objective of the present study was to clarify the expression characteristics of long non­coding RNA (lncRNA) FGD5 antisense RNA 1 (FGD5­AS1) in pancreatic cancer, as well as its biological function and underlying mechanism. Reverse transcription­quantitative polymerase chain reaction (RT­qPCR) was utilized for the detection of FGD5­AS1 and microRNA (miR)­577 expression levels in pancreatic cancer tissues. Transfection was performed to upregulate or downregulate FGD5­AS1 in pancreatic cancer cell lines. MTT and Transwell assays were then utilized to detect the proliferation, migration and invasion of cancer cells, respectively. Subsequently, dual­luciferase reporter gene assay, RNA immunoprecipitation assay, RNA pull­down assay, RT­qPCR, western blotting, and Pearson's correlation analysis were employed to confirm the regulatory relationships among FGD5­AS1, miR­577, low­density lipoprotein receptor­related protein 6 (LRP6) and ß­catenin. Western blotting was employed to determine the expression levels of Axin2, cyclin D1 and c­Myc. The expression level of FGD5­AS1 was upregulated in pancreatic cancer tissues and cell lines. FGD5­AS1 knockdown inhibited pancreatic cancer cell proliferation, migration and invasion. By contrast, miR­577 was significantly inhibited in pancreatic cancer cells and tissues; its downregulation promoted pancreatic cancer cell proliferation, migration and invasion, and reversed the effects of FGD5­AS1 knockdown on pancreatic cancer cells. In addition, it was revealed that miR­577 was a target of FGD5­AS1, and FGD5­AS1 could modulate the expression levels of LRP6, ß­catenin, Axin2, cyclin D1 and c­Myc via suppressing miR­577. In conclusion, in pancreatic cancer, highly expressed FGD5­AS1 activated the Wnt/ß­catenin signaling and promoted cancer cell proliferation, migration and invasion via suppression of miR­577.

A Bifunctional Coordination Polymer for Al3+ Ion Detection and its Treatment Effect on Nonalcoholic Fatty Liver Disease by Promoting Lipoxygenases and Reducing Intrahepatic Triglyceride Content.

In this study, a new Cd(II)-bearing coordination polymer with the chemical formula of {[Cd4(meda)3(dpe)4(H2O)4]·(NO3)2·2(H2O)}n (1, H2meda = 3,3'-methylenedibenzoic acid, dpe = 1,2-di(pyridin-4-yl)ethane) has been successfully prepared by reaction of Cd(NO3)·4H2O with a V-shape carboxyl ligand H2meda along with the linear dipyridine ligand dpe under the hydrothermal conditions. Due to its intensive luminescence, complex 1 could be utilized as the sensor of detecting Al3+ ion, and its detection limit is 4 × 10-6 M. Firstly, the toxicity of the compound on the normal liver cells was determined with Cell Counting Kit-8 detection kit. The triglyceride in liver cells was detected by detection kit after compound treatment and the relative expression of 15-lox and 12-lox in L02 cells was also measured by RT-PCR after compound treatment. In addition, multiple functional groups that provided by the synthesized Cd(II) complex have been studied by using molecular docking simulation for the confirmation of possible binding modes that formed between ligand and receptor.

Efficacy of surgical treatment on different sizes of hepatitis B virus-related hepatocellular carcinoma and prognostic analysis.

PURPOSE: To investigate the efficacy of surgical resection for patients with different sizes of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), and to analyze the risk factors influencing the prognosis. METHODS: The clinical data of a total of 138 patients with HBV-related HCC admitted to and treated in our hospital from June 2012 to June 2014 were retrospectively analyzed, and the patients were divided into small HCC (SHCC) group (tumor diameter ≤5 cm, n=69) and solitary large HCC (SLHCC) group (tumor diameter >5 cm, n=69) based on the size of tumors. The differences in operative methods, operation time, intraoperative blood loss, number of intraoperative blood transfusion, time of portal triad clamping and incidence of complications, as well as postoperative liver function and alpha fetoprotein (AFP) indexes, tumor recurrence and survival conditions were compared between the two groups. RESULTS: Among the 138 HCC patients who underwent hepatectomy, 54 cases had ≥3 resected hepatic segments, and 84 cases had <3 resected hepatic segments. SHCC group exhibited remarkably shorter operation time and notably smaller intraoperative blood loss than SLHCC group. The 1-, 3- and 5-year overall survival rates were 91.3%, 87.0%, 71.0%, 60.9%, 58.0% and 46.4%, respectively, and the 1-, 3- and 5-year disease-free survival rates were 71.0%, 63.8%, 47.8%, 44.9%, 37.7% and 30.4%, respectively, in the two groups. The log-rank test showed that the overall survival rate in SHCC group was distinctly higher than that in SLHCC group (p=0.041), and no statistically significant difference in the disease-free survival rate was detected. According to multivariate analysis, HBV deoxyribonucleic acid (DNA) load ≥104 U/mL, tumor diameter >5 cm and positive microvascular invasion were independent risk factors for the patient's prognosis (p<0.05). CONCLUSIONS: SLHCC has a similar disease-free survival rate to SHCC but a lower overall survival rate than SHCC. HBV DNA load ≥104 U/mL, tumor diameter >5 cm and positive microvascular invasion are independent risk factors for the patient's prognosis.

Diabetes mellitus and risk of pancreatic cancer in China: A meta-analysis based on 26 case-control studies.

AIMS: The relationship between diabetes mellitus and pancreatic cancer risk from is uncertain based on the results of existing publications. The current report updated and re-evaluated the possible association between diabetes mellitus and pancreatic cancer risk in China. METHODS: Six databases (PubMed, Embase, Web of Science, the Cochrane Library, the Chinese Biomedical Database, and the Chinese National Knowledge Infrastructure) were used for the literature search up to October 2017. RESULTS: Twenty-six case-control studies involving 7702 pancreatic cancer cases and 10186 controls were screened out. The overall summary estimate for the relationship between diabetes and pancreatic cancer was 3.69 (95% CI, 3.12-4.37). The subgroup analysis indicated positive associations among northern and southern Chinese, as well as studies with healthy population or hospital controls. In addition, the risk of developing pancreatic cancer was inversely associated with the duration of diabetes, with the highest risk of pancreatic cancer occurring among patients with diabetes <2years. Individuals who had diabetes <2years had a >2-fold higher risk of developing pancreatic cancer than individuals who had diabetes for 2-4years or 5-10years (OR, 4.92; 95% CI, 4.16-5.80 vs. OR, 1.92; 95% CI, 1.30-2.85/OR, 2.14; 95% CI, 1.49-3.09). CONCLUSIONS: This meta-analysis strongly supports that an association exists between diabetes and an increased risk of pancreatic cancer in China, which should be confirmed with other ethnic groups.