RESUMO
The objective of this study was to assess the genetic diversity and population structure of goats in the Yangtze River region using microsatellite and mtDNA to better understand the current status of those goat genetic diversity and the effects of natural landscape in fashion of domestic animal genetic diversity. The genetic variability of 16 goat populations in the littoral zone of the Yangtze River was estimated using 21 autosomal microsatellites, which revealed high diversity and genetic population clustering with a dispersed geographical distribution. A phylogenetic analysis of the mitochondrial D-loop region (482 bp) was conducted in 494 goats from the Yangtze River region. In total, 117 SNPs were reconstructed, and 173 haplotypes were identified, 94.5% of which belonged to lineages A and B. Lineages C, D, and G had lower frequencies (5.2%), and lineage F haplotypes were undetected. Several high-frequency haplotypes were shared by different ecogeographically distributed populations, and the close phylogenetic relationships among certain low-frequency haplotypes indicated the historical exchange of genetic material among these populations. In particular, the lineage G haplotype suggests that some west Asian goat genetic material may have been transferred to China via Muslim migration.
RESUMO
The Hechuan white goat (Capra hircus), an indigenous of China. Here, we describe the complete mitochondrial genome sequence of Hechuan white goat. The mitogenome is 16,640 nt in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. Its overall base composition is A: 33.5%, T: 27.3%, C: 26.1% and G: 13.1%. The complete mitogenome of the local subspecies of Hechuan white goat could provide an important data to further explore the breed improvement in Chinese goat.
Assuntos
Genoma Mitocondrial , Cabras/genética , Animais , Composição de Bases , NADH Desidrogenase/genética , Fases de Leitura Aberta , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The Youzhou black-skin goat (Capra hircus), an indigenous breed of Chinese southwest. Here, we describe the complete mitochondrial genome sequence of Hechuan white goat. The mitogenome is 16,640 nt in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. Its overall base composition is A: 33.5%, T: 27.3%, C: 26.1% and G: 13.1%. The complete mitogenome of the local subspecies of Hechuan white goat could provide an important data to further breed improvement and animal genetics resource conservation in China.
Assuntos
Genoma Mitocondrial , Cabras/genética , Animais , Composição de Bases , Fases de Leitura Aberta , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
Dazu Black goat is an indigenous goat genetic resource in Southwest of China. Here, we describe its complete mitochondrial genome sequence. The mitogenome is 16,641 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. Its overall base composition is A: 33.5%, T: 27.3%, C: 26.1% and G: 13.1%. The complete mitogenome of the indigenous goat could provide important data to further explore the taxonomic status of the subspecies and also provide a starting point for further phylogenetic studies.
Assuntos
Genoma Mitocondrial , Cabras/genética , Animais , Composição de Bases , NADH Desidrogenase/genética , Fases de Leitura Aberta , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The Chuanzhong black goat (Capra hircus) is a breed native to southwest of China. Its complete mitochondrial genome is 16,641 nt in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a non-coding control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. Its overall base composition is A: 33.5%, T: 27.3%, C: 26.1%, and G: 13.1%. The complete mitogenome of the Chinese indigenous breed of goat could provide a basic data for further phylogenetics analysis.
Assuntos
Genoma Mitocondrial , Cabras/classificação , Cabras/genética , Animais , Composição de Bases , Cruzamento , China , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Filogenia , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
Here, we describe the complete mitochondrial genome sequences of Jining Gray goat, Fushun black goat, Youzhou black-skin goat, and Hechuan white goat. The mitogenome of those four goats consisted of 16,640 nt, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes, which are encoded on the light strand. The complete mitogenome of these four local breeds of Chinese native goats could provide an important data to further breed improvement and animal genetics resource conservation in China.
Assuntos
Genoma Mitocondrial , Cabras/genética , Animais , DNA Intergênico/química , DNA Intergênico/genética , Fases de Leitura Aberta , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
Although expression and epigenetic differences of imprinted genes have been extensively characterised in man and the mouse, little is known on livestock species. In this study, the polymorphism-based approach was used to detect the imprinting status of NNAT and DIRAS3 genes in five heterozygous pigs (based on SNP) of Large White and Meishan F(1) hybrids. The results show that both genes were paternally expressed in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). In addition, the NNAT gene had two transcripts in all tested tissues, which is consistent with its counterpart in man and cattle.
Assuntos
Impressão Genômica , Proteínas de Membrana/genética , Proteínas do Tecido Nervoso/genética , Sus scrofa/genética , Proteínas rho de Ligação ao GTP/genética , Animais , Sequência de Bases , Bovinos , DNA/genética , Primers do DNA/genética , Feminino , Expressão Gênica , Humanos , Masculino , Dados de Sequência Molecular , Polimorfismo de Nucleotídeo Único , Especificidade da Espécie , Distribuição TecidualRESUMO
The genetic variation of Meishan, Huainan, Tongcheng, Bamei, Hezuo and Largewhite pigs were analyzed by RAPD markers. Twenty-four single polymorphic primers were selected out of 276 primers by amplifying six pool DNA. The index of Shannon were 0.178672, 0.17781,0.15995, 0.14549,0.16949, 0.14159 respectively; Hpop was 0.16216, Hsp was 0.2534. The phylogenetic tree was constructed using NJ and UPGMA. The results indicated that the phenylogenetic relationship of the six pig breeds was consistent with their distribution.