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1.
Andrologia ; 50(2)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29057495

RESUMO

Varicocele (VC) is a common cause of male infertility, but the molecular mechanisms involved in its pathogenesis are unknown. We investigated the impact of varicocelectomy (VCT) on proteome profiles in testicular tissues of rats with VC, and analysed associated target genes and signalling pathways. Sixty male rats with VC were divided into two groups: control (n = 30), and VCT (n = 30). Tissues were collected 4 weeks after sham or VCT surgery. Matrix-assisted laser desorption/ionisation time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) was used to analyse the comparative proteome profiles. Kyoto Encyclopaedia of Genes and Genomes (KEGG) Orthology-Based Annotation System was used for bioinformatic analysis. Fifteen proteins were differentially expressed between control and VCT groups. These differentially expressed proteins are associated with several specific cellular processes associated with the pathogenesis of testicular growth arrest associated with VC. Furthermore, the evaluation by transmission electron micrograph showed that VCT could decrease apoptosis of spermatogenic cells in rats. Understanding such molecular pathways might provide physicians with a better insight into VC and with potential targets for treatment.


Assuntos
Infertilidade Masculina/patologia , Proteoma , Testículo/metabolismo , Procedimentos Cirúrgicos Urológicos Masculinos/efeitos adversos , Varicocele/cirurgia , Animais , Apoptose , Biologia Computacional , Modelos Animais de Doenças , Células Germinativas/ultraestrutura , Humanos , Infertilidade Masculina/etiologia , Masculino , Microscopia Eletrônica de Transmissão , Proteômica/métodos , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Testículo/citologia , Testículo/patologia , Procedimentos Cirúrgicos Urológicos Masculinos/métodos , Varicocele/complicações , Varicocele/patologia
2.
Genet Mol Res ; 15(2)2016 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-27421023

RESUMO

Bama Xiang and Landrace pigs are the local fatty and lean breeds, respectively, in China. We compared differences in carcass traits, meat quality traits, and myosin heavy chain (MyHC) types in the longissimus dorsi muscles between Bama Xiang and Landrace pigs. This was done in pigs of the same age, using real-time PCR, to investigate the relationship between MyHC fiber types and carcass characteristics, meat quality traits, and the key factors regulating muscle fiber type. Bama Xiang pigs exhibited smaller size and slower growth than Landrace pigs (P < 0.01). We found that the superior meat quality, especially the high intramuscular fat (IMF) content in Bama Xiang pig, was related to elevated type I oxidative muscle fiber content (P < 0.01). In contrast, Landrace pig muscle had a higher glycolytic type IIb muscle fiber content (P < 0.01). MyHC I gene expression was significantly positively correlated with backfat thickness and IMF content (P < 0.01). MyHC IIb was significantly negatively correlated with IMF content (P < 0.05), and positively correlated with carcass yield (P < 0.05). AMP-activated protein kinase and peroxisome proliferator-activated receptor-g coactivator-1a are suggested to be the two key factors regulating muscle fiber type in pigs. Our results indicate that muscle fiber composition is one of the key differences leading to the differences of meat quality between Bama Xiang and Landrace pigs. These results may provide a theoretical basis for further studies of the molecular mechanism underlying the excellent meat quality of the Bama Xiang pig.


Assuntos
Carne/normas , Fibras Musculares Esqueléticas/fisiologia , Cadeias Pesadas de Miosina/genética , Suínos/fisiologia , Proteínas Quinases Ativadas por AMP/genética , Animais , Peso Corporal/genética , Cruzamento , China , Expressão Gênica , Fibras Musculares Esqueléticas/metabolismo , Cadeias Pesadas de Miosina/biossíntese , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/genética , RNA Mensageiro/genética , Suínos/metabolismo , Fatores de Transcrição/genética
3.
Genet Mol Res ; 15(2)2016 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-27323106

RESUMO

Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism. This study investigated LPL gene expression, LPL enzyme activity, and the correlation of each with intramuscular fat (IMF) in Chinese Guangxi san-huang (GXSH) and Arbor Acres (AA) chickens. The results showed that age and breed had significant effects on LPL expression and enzyme activity. Correlation analyses showed significant positive correlations between LPL expression levels and IMF contents in the breast and thigh tissues of both GXSH (r = 0.712, P = 0.001; r = 0.792, P < 0.001, respectively) and AA (r = 0.644, P < 0.001; r = 0.545, P < 0.001, respectively) chickens. The results also indicated a significant positive correlation between LPL enzyme activity and IMF contents in the breast and thigh tissues of both GXSH (r = 0.615, P = 0.001; r = 0.685, P < 0.001, respectively) and AA (r = 0.600, P = 0.001; r = 0.528, P = 0.003, respectively) chickens. The results indicated that the LPL gene was significantly correlated with IMF in these two breeds. The results presented here could contribute to knowledge of LPL mRNA developmental expression patterns and enzyme activity, and it could facilitate further research on the molecular mechanisms underlying IMF deposition in chickens.


Assuntos
Galinhas/genética , Lipase Lipoproteica/genética , Carne/normas , Tecido Adiposo/metabolismo , Animais , Lipase Lipoproteica/metabolismo , Músculo Esquelético/metabolismo
4.
Int J Impot Res ; 28(4): 127-32, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27193064

RESUMO

To assess the efficacy and mechanism of circumcision in the treatment of premature ejaculation (PE) with redundant prepuce, we enrolled a total of 81 PE patients who received circumcision. The patients' ejaculatory ability and sexual performances were evaluated before and after circumcision by using questionnaires (Intravaginal ejaculation latency time (IELT), Chinese Index of PE with 5 questions (CIPE-5) and International Index of Erectile function- 5 (IIEF-5)). Furthermore, somatosensory evoked potentials (SEPs) including dorsal nerve (DNSEP) and glans penis (GPSEP) of the patients were also measured. The mean IELTs of preoperation and post operation were 1.10±0.55 and 2.48±2.03 min, respectively (P<0.001). In addition, the geometric mean IELT after operation was 2.16 min, compared with the baseline 1.07 min before the operation, the fold increase of the IELT was 2.02. Compared with the uncircumcised status, scores of CIPE-5 showed a significant increase after circumcision (P<0.001). The mean latencies (and amplitudes) of GPSEP and DNSEP were 38.1±4.0 ms (3.0±1.9 uV) and 40.5±3.4 ms (2.8±1.6 uV) before circumcision, respectively; and 42.8±3.3 ms (2.8±1.6 uV) and 40.5±4.1 ms (2.4±1.2 uV) in the follow-up end point after circumcision. Only the latencies of GPSEP showed significant prolongation before and after circumcision (P<0.001). The ejaculation time improvement after circumcision is so small, and equal to placebo response, therefore it could not be interpreted as a therapeutic method in men with PE.


Assuntos
Potenciais Somatossensoriais Evocados/fisiologia , Ereção Peniana/fisiologia , Pênis/inervação , Ejaculação Precoce/cirurgia , Comportamento Sexual/fisiologia , Adulto , Circuncisão Masculina , Ejaculação/fisiologia , Humanos , Masculino , Ejaculação Precoce/fisiopatologia , Inquéritos e Questionários , Resultado do Tratamento , Adulto Jovem
5.
Clin Radiol ; 71(5): 471-5, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26944699

RESUMO

AIM: To evaluate retrospectively the incidence and predictors of massive bleeding after stent placement for malignant oesophageal stricture/fistulae. MATERIALS AND METHODS: This retrospective study comprised 519 patients with malignant oesophageal stricture/fistulae that were successfully treated with stent placement at three hospitals. The patients were divided into two groups based on the occurrence of massive bleeding. Univariate and multivariate analysis was performed to evaluate predictive factors of massive bleeding. RESULTS: Massive bleeding occurred in 54 of 519 patients 1-37 days following stent placement. All of the patients who developed massive bleeding died within 24 hours of the event. Univariate analysis showed massive bleeding was associated with the presence of a concomitant tracheal stent (p<0.001), the existence of concomitant oesophageal fistulae (p<0.001), and prior radiotherapy (p<0.001). Multivariate analysis exhibited that concomitant tracheal stent insertion (odds ratio [OR], 23.134; 95% confidence interval [CI], 9.523-56.199; p<0.001), the presence of oesophageal fistulae (OR, 3.724; 95% CI, 1.677-8.269; p=0.001), and prior radiotherapy (OR, 13.310; 95% CI, 5.464-32.421; p<0.001) were predictors of massive bleeding following stenting. CONCLUSIONS: The presence of oesophageal fistulae, prior radiotherapy, and concomitant tracheal stent are important factors contributing to bleeding after stenting.


Assuntos
Doenças do Esôfago/etiologia , Fístula Esofágica/cirurgia , Neoplasias Esofágicas/complicações , Estenose Esofágica/cirurgia , Hemorragia Gastrointestinal/etiologia , Stents/efeitos adversos , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Esofágicas/radioterapia , Feminino , Previsões , Hemorragia Gastrointestinal/mortalidade , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Estudos Retrospectivos
6.
Andrologia ; 48(9): 874-881, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26781087

RESUMO

The purpose of this study was to evaluate the predictors of pregnancy outcome for infertile couples attending in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) programmes. Infertile couples attending IVF or ICSI procedures were included in this study. Related data including semen parameters and male and female age and body mass index were collected and analysed. The main outcome was clinical pregnancy, defined as an ultrasound detection of foetal heartbeat 6 weeks after embryo transfer. A total of 1316 couples who underwent IVF and 266 who underwent ICSI were recruited for this study. A multivariate logistic regression with likelihood ratio test revealed the following predictors of pregnancy outcome: female age and sperm DNA fragmentation index (DFI) and acrosomal activity in IVF procedures (chi-square of likelihood ratio = 26.42, d.f. = 3, P < 0.005) and female age and DFI in ICSI procedures (chi-square of likelihood ratio = 18.88, d.f. = 2, P < 0.005). In conclusion, our study indicated that sperm DFI, female age and acrosomal levels have a significant effect on ART pregnancy outcome.


Assuntos
Fertilização in vitro , Infertilidade/terapia , Injeções de Esperma Intracitoplásmicas , Reação Acrossômica , Adulto , Fragmentação do DNA , Transferência Embrionária , Feminino , Humanos , Funções Verossimilhança , Masculino , Idade Materna , Gravidez , Resultado da Gravidez , Taxa de Gravidez
7.
Genet Mol Res ; 14(2): 3234-43, 2015 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-25966089

RESUMO

In the present study, the complete mitochondrial (mt) genome of Cyclemys dentata was determined using PCR reactions. The structural organization and gene order of C. dentata were equivalent to those of most other vertebrates. The mt genome was 16,489 bp in length, has rich A+T content, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region (D-loop). All protein-coding genes started with ATG, many genes have complete stop codons, except ND2, COX3, ND3, and cyt-b genes had incomplete stop codons of T. The light-strand replication origin (OL) of C. dentata might fold into a stable stem-loop secondary structure, and its loop had 2 nt less than that of the Cyclemys atripons OL sequence. The D-Loop of C. dentata contained a central domain (CD), 2 extended termination associated sequences (ETAS1, ETAS2) and 3 conserved sequence blocks (CSB1, CSB2, CSB3). The average length of 20 turtles' mt genomes was 16,692.5 bp, including 34.1% A, 27.0% T, 26.0% C and 12.9% G. The C. dentata mitochondrial genome could provide useful data for further studies on phylogenetics and conservation genetics of this species. The phylogenetic relationships of the family Geoemydidae were analyzed by maximum-likelihood (ML) and neighbor-joining (NJ) based on concatenated sequences of 13 protein-coding genes from 20 turtle species. The ML and NJ trees had homologous topologies. The results support the existing classification of the genera of Geoemydidae, that C. dentata was a sister species of C. atripons, Pyxidea nested in Cuora, and Chinemys was synonymous with Mauremys.


Assuntos
DNA Mitocondrial/genética , Genoma Mitocondrial/genética , Filogenia , Tartarugas/genética , Animais , Sequência de Bases , DNA Circular/química , DNA Circular/genética , DNA Mitocondrial/química , DNA Mitocondrial/classificação , Ordem dos Genes , Genes Mitocondriais/genética , Proteínas Mitocondriais/genética , Dados de Sequência Molecular , Análise de Sequência de DNA , Tartarugas/classificação
8.
Reprod Fertil Dev ; 23(6): 788-97, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21791180

RESUMO

This study was carried out to test the feasibility of enhancing embryo production in vivo and in vitro by immunoneutralisation against inhibin or follistatin. In Experiment 1, multi-parity buffaloes were assigned into three groups: High group (n=8), which received one primary (2mg) and two booster (1mg) vaccinations (28-day intervals) with a recombinant inhibin α subunit in 1 mL of white oil adjuvant; Low group (n=8), which received half that dose; and Control group (n=7), which received only adjuvant. Immunisation against inhibin stimulated development of ovarian follicles. Following superovulation and artificial insemination, inhibin-immunised buffaloes had more developing follicles than the Control buffaloes. The average number of embryos and unfertilised ova (4.5±0.6, n=6) in the High group was higher (P<0.05) than in the Control group (2.8±0.6, n=5) and was intermediate (4.1±0.7, n=7) in the Low group. The pooled number of transferable embryos of the High and Low groups (3.2±0.5, n=13) was also higher (P<0.05) than that (1.6±0.7, n=5) of the controls. The immunised groups also had higher plasma concentrations of activin, oestradiol and progesterone. In Experiment 2, the addition of anti-inhibin or anti-follistatin antibodies into buffalo oocyte IVM maturation medium significantly improved oocyte maturation and cleavage rates following parthenogenic activation. Treatment with anti-follistatin antibody also doubled the blastocyst yield from activated embryos. These results demonstrated that immunisation against inhibin stimulated follicular development, enhanced oocyte quality and maturation competence, yielded more and better embryos both in vivo and in vitro.


Assuntos
Cruzamento/métodos , Búfalos/fisiologia , Imunização/veterinária , Inibinas/imunologia , Oócitos/fisiologia , Folículo Ovariano/crescimento & desenvolvimento , Proteínas Recombinantes/imunologia , Superovulação/fisiologia , Ativinas/sangue , Animais , Anticorpos/sangue , Anticorpos/imunologia , Búfalos/imunologia , Ensaio de Imunoadsorção Enzimática , Estradiol/sangue , Feminino , Folistatina/imunologia , Inseminação Artificial , Oócitos/citologia , Folículo Ovariano/diagnóstico por imagem , Progesterona/sangue , Subunidades Proteicas/imunologia , Ultrassonografia
9.
Eur J Surg Oncol ; 30(5): 531-5, 2004 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15135482

RESUMO

AIMS: Hereditary non-polyposis colorectal cancer, thyroid medullary carcinoma, breast/ovarian cancer and gastric cancer/breast cancer syndrome are encountered in surgery. Some gastric cancer/breast cancer syndrome may be the result of a CDH1 germline mutation. This is the first report of CDH1 germline mutations gastric cancer/breast cancer syndrome in Chinese patients. METHODS: Peripheral blood from the proband, as well as, her first and second degree relatives was collected and CDH1 gene exon 1-16 mutations were screened. E-cadherin/beta-catenin proteins expression and histopathologic features were examined on gastric cancer/breast cancer tissues from the proband. RESULTS: A C-->T nucleotide substitution at exon 13 (mRNA 2200 locus, Accession number NM-004360) was found. This was a transition from GCC-->GCT in DNA sequence (Ala154Ala). Diffuse-type gastric cancer and infiltrating ductal breast carcinoma were present. Both tumours preserved E-cadherin/beta-catenin expression immunohistochemically. CONCLUSIONS: Familial cancer syndrome with diffuse-type gastric cancer/breast cancer proband in Chinese has a propensity of early onset during lifespan. No truncating or splice-site CDH1 mutations had been identified in this family. A silent nucleotide variation in exon 13 of the CDH1 gene may contribute to some forms of cancer susceptibility.


Assuntos
Povo Asiático/genética , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Desidrogenases de Carboidrato/genética , Carcinoma Ductal/genética , Carcinoma Ductal/patologia , Mutação em Linhagem Germinativa/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Adulto , Substituição de Aminoácidos/genética , Sequência de Bases , Neoplasias da Mama/metabolismo , Caderinas/metabolismo , Carcinoma Ductal/metabolismo , China , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Proteínas do Citoesqueleto/metabolismo , DNA de Neoplasias/genética , Éxons/genética , Feminino , Predisposição Genética para Doença/genética , Humanos , Imuno-Histoquímica , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Masculino , Pessoa de Meia-Idade , Linhagem , Reação em Cadeia da Polimerase , Polimorfismo Conformacional de Fita Simples , Neoplasias Gástricas/metabolismo , Síndrome , Transativadores/metabolismo , beta Catenina
10.
Hinyokika Kiyo ; 37(10): 1135-40, 1991 Oct.
Artigo em Japonês | MEDLINE | ID: mdl-1721766

RESUMO

Spontaneous plaque-forming cell, SPFC, is a new hemolytic effector of monocytes, which is generated 6 to 7 days after antigen unstimulation or stimulation in man. SPFC is also detected in rats. A significant concomitant response of the SPFC, in number of SPFC at the peak response around the rejection day, and histoincompatibility of allogeneic combinations in skin or heart allograft transplantation of rats was found. Peak number of SPFC (28,850 + 1,343/10(6) on day 6 in H-1 incompatible combination (ACI-Lewis) was higher than that (11,606 + 4,235/10(6)) on day 10 in H-1 compatible combination (F344-Lewis) (p less than 0.05, Student'st-test). The day of peak SPFC response in each experimental group was also concordant with the day of rejection. The OX42 monoclonal antibody against CR3 of leukocyte-adhesion molecules inhibited hemolysis of the SPFC. Since the erythrocyte is a ligand of CR3, it is likely that hemolysis of SPFC is mediated by the CR3 adhesion molecules. In conclusion, SPFC may be an immunologic indicator of allograft rejection in rats.


Assuntos
Eritrócitos/citologia , Rejeição de Enxerto/imunologia , Hemólise , Monócitos/imunologia , Animais , Moléculas de Adesão Celular/metabolismo , Complemento C3/metabolismo , Eritrócitos/imunologia , Eritrócitos/metabolismo , Transplante de Coração/imunologia , Técnica de Placa Hemolítica , Selectina L , Masculino , Monócitos/metabolismo , Ratos , Ratos Endogâmicos F344 , Ratos Endogâmicos Lew , Receptores de Complemento/metabolismo , Transplante de Pele/imunologia , Transplante Homólogo
11.
Zhonghua Zhong Liu Za Zhi ; 9(2): 133-5, 1987 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-2820682

RESUMO

Twenty one patients with bronchial adenoma, treated surgically in our hospital, include three different neoplasms: carcinoid, cylindroma and mucoepidermoid adenoma. In this series, clinical characteristics were: bronchial obstruction when the tumor protrudes into the lumen and infection, first in the bronchus (bronchitis or bronchiectasis) then in the parenchyma (acute, recurrent or chronic pneumonia, tension abscess). Ball-valve action of the tumor may result in lobar or segmental emphysema. Preoperatively, most of the patients had been misdiagnosed as bronchitis, carcinoma of lung, bronchiectasis or acute tension abscess. In our series, all the patients were alive in a follow up of 2-8 years. Yet one patient is living with local recurrence and distant metastasis. To our experience, pneumonia recurring in the same area of the lung, localized wheezing, with or without endocrine symptoms, lobar or segmental emphysema may suggest bronchial adenoma. Tomography and endoscopy are important for diagnosis. For the treatment, sleeve resection of the main bronchus was done in 2, bronchoplastic lobectomy in 7, lobectomy in 10, and pneumonectomy in 2. Sleeve resection of the main bronchus or bronchoplastic lobectomy is recommended as a reliable procedure for this disease.


Assuntos
Adenoma/diagnóstico , Neoplasias Brônquicas/diagnóstico , Adenoma/cirurgia , Adolescente , Adulto , Neoplasias Brônquicas/cirurgia , Tumor Carcinoide/diagnóstico , Tumor Carcinoide/cirurgia , Carcinoma Adenoide Cístico/diagnóstico , Carcinoma Adenoide Cístico/cirurgia , Erros de Diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
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