Poxvirus-based vector systems and the potential for multi-valent and multi-pathogen vaccines.

INTRODUCTION: With the increasing number of vaccines and vaccine-preventable diseases, the pressure to generate multi-valent and multi-pathogen vaccines grows. Combining individual established vaccines to generate single-shot formulations represents an established path, with significant ensuing public health and cost benefits. Poxvirus-based vector systems have the capacity for large recombinant payloads and have been widely used as platforms for the development of recombinant vaccines encoding multiple antigens, with considerable clinical trials activity and a number of registered and licensed products. AREAS COVERED: Herein we discuss design strategies, production processes, safety issues, regulatory hurdles and clinical trial activities, as well as pertinent new technologies such as systems vaccinology and needle-free delivery. Literature searches used PubMed, Google Scholar and clinical trials registries, with a focus on the recombinant vaccinia-based systems, Modified Vaccinia Ankara and the recently developed Sementis Copenhagen Vector. EXPERT COMMENTARY: Vaccinia-based platforms show considerable promise for the development of multi-valent and multi-pathogen vaccines, especially with recent developments in vector technologies and manufacturing processes. New methodologies for defining immune correlates and human challenge models may also facilitate bringing such vaccines to market.

Perspectives for the use of silver nanoparticles in dental practice.

Nanotechnology has been used for medical applications in several forms, including dental practice with the development of silver nanoparticles (Ag NPs) as a useful tool. The aim of this review was to identify the properties and appliances of Ag NPs in dental practice. Silver compounds and NPs have already been used as dental restorative material, endodontic retrofill cements, dental implants and caries inhibitory solution. Despite the effectiveness that Ag NPs has shown in dental practice, Ag NPs remain a controversial area of research with respect to their toxicity in biological and ecological systems. Therefore any application of Ag NPs in dentistry requires more studies. In order to avoid the toxicity of these materials Ag NPs can be temporarily used in dentistry.