Common use herbicides increase wetland greenhouse gas emissions.

Wetlands play a disproportionate role in the global climate as major sources and sinks of greenhouse gases. Herbicides are the most heavily used agrochemicals and are frequently detected in aquatic ecosystems, with glyphosate and 2,4-Dichlorophenoxyacetic acid (2,4-D), representing the two most commonly used worldwide. In recent years, these herbicides are being used in mixtures to combat herbicide-tolerant noxious weeds. While it is well documented that herbicide use for agriculture is expected to increase, their indirect effects on wetland greenhouse gas dynamics are virtually unknown. To fill this knowledge gap, we conducted a factorial microcosm experiment using low, medium, and high concentrations of glyphosate or 2,4-D, individually and in combination to investigate their effects on wetland methane, carbon dioxide, and nitrous oxide fluxes. We predicted that mixed herbicide treatments would have a synergistic effect on greenhouse gases compared to individual herbicides. Our results showed that carbon dioxide flux rates and cumulative emissions significantly increased from both individual and mixed herbicide treatments, whereas methane and nitrous oxide dynamics were less affected. This study suggests that extensive use of glyphosate and 2,4-D may increase carbon dioxide emissions from wetlands, which could have implications for climate change.

Practical Guide to Measuring Wetland Carbon Pools and Fluxes.

Wetlands cover a small portion of the world, but have disproportionate influence on global carbon (C) sequestration, carbon dioxide and methane emissions, and aquatic C fluxes. However, the underlying biogeochemical processes that affect wetland C pools and fluxes are complex and dynamic, making measurements of wetland C challenging. Over decades of research, many observational, experimental, and analytical approaches have been developed to understand and quantify pools and fluxes of wetland C. Sampling approaches range in their representation of wetland C from short to long timeframes and local to landscape spatial scales. This review summarizes common and cutting-edge methodological approaches for quantifying wetland C pools and fluxes. We first define each of the major C pools and fluxes and provide rationale for their importance to wetland C dynamics. For each approach, we clarify what component of wetland C is measured and its spatial and temporal representativeness and constraints. We describe practical considerations for each approach, such as where and when an approach is typically used, who can conduct the measurements (expertise, training requirements), and how approaches are conducted, including considerations on equipment complexity and costs. Finally, we review key covariates and ancillary measurements that enhance the interpretation of findings and facilitate model development. The protocols that we describe to measure soil, water, vegetation, and gases are also relevant for related disciplines such as ecology. Improved quality and consistency of data collection and reporting across studies will help reduce global uncertainties and develop management strategies to use wetlands as nature-based climate solutions. Supplementary Information: The online version contains supplementary material available at 10.1007/s13157-023-01722-2.

Repeated large-scale mechanical treatment of invasive Typha under increasing water levels promotes floating mat formation and wetland methane emissions.

Invasive species management typically aims to promote diversity and wildlife habitat, but little is known about how management techniques affect wetland carbon (C) dynamics. Since wetland C uptake is largely influenced by water levels and highly productive plants, the interplay of hydrologic extremes and invasive species is fundamental to understanding and managing these ecosystems. During a period of rapid water level rise in the Laurentian Great Lakes, we tested how mechanical treatment of invasive plant Typha × glauca shifts plant-mediated wetland C metrics. From 2015 to 2017, we implemented large-scale treatment plots (0.36-ha) of harvest (i.e., cut above water surface, removed biomass twice a season), crush (i.e., ran over biomass once mid-season with a tracked vehicle), and Typha-dominated controls. Treated Typha regrew with approximately half as much biomass as unmanipulated controls each year, and Typha production in control stands increased from 500 to 1500 g-dry mass m-2 yr-1 with rising water levels (~10 to 75 cm) across five years. Harvested stands had total in-situ methane (CH4) flux rates twice as high as in controls, and this increase was likely via transport through cut stems because crushing did not change total CH4 flux. In 2018, one year after final treatment implementation, crushed stands had greater surface water diffusive CH4 flux rates than controls (measured using dissolved gas in water), likely due to anaerobic decomposition of flattened biomass. Legacy effects of treatments were evident in 2019; floating Typha mats were present only in harvested and crushed stands, with higher frequency in deeper water and a positive correlation with surface water diffusive CH4 flux. Our study demonstrates that two mechanical treatments have differential effects on Typha structure and consequent wetland CH4 emissions, suggesting that C-based responses and multi-year monitoring in variable water conditions are necessary to accurately assess how management impacts ecological function.