Potential group B Streptococcus interspecies transmission between cattle and people in Colombian dairy farms.

Group B Streptococcus (GBS), is a leading cause of neonatal death and an emerging pathogen in adults. Additionally, GBS is a bovine pathogen causing intramammary infections. The likelihood of GBS interspecies transmission is largely unknown. We explored the potential transmission of GBS between cattle and people on dairy farms in Colombia and compared the antimicrobial resistance (AMR) profiles of isolates from both host species. Across 33 farms, throat swabs and rectal swabs were collected from 191 people, and rectal swabs and composite milk samples from 2092 cattle, yielding 60 human isolates and 301 bovine isolates. The majority (64%) of isolates belonged to shared sequence types (ST). Sequence type (ST) 1 was the most common strain in both host species, suggesting that interspecies transmission may be possible. Two members of the bovine-specific clonal complex 61/67 were detected in human samples (ST718 and ST1175), providing evidence for the lack of genuine species barriers. Apparent prevalence of penicillin resistance was surprisingly high in human and bovine isolates. Further investigation of this phenomenon is needed and could lead to modification of standard testing and treatment recommendations in human and veterinary medicine.

Validación de contenido y adaptación del cuestionario Fantastico por técnica Delphi / Validation and adaptation of the Fantastico questionnaire by Delphi technique

Objetivo: Realizar la validación de contenido y adaptación del cuestionario FANTASTICO a través de la técnica Delphi para caracterizar los estilos de vida en adolescentes escolarizados. Materiales y métodos: Estudio de validación y adaptación con 12 expertos utilizando la técnica Delphi de consenso a tres rondas; un Alpha de Cronbach > 0,80 se consideró como valor óptimo de fiabilidad para la pertinencia, relevancia, utilidad, redacción y claridad. Resultados: Los 30 ítems incluidos en el cuestionario evalúan conductas en diez dimensiones del estilo de vida−familiares y amigos, asociatividad y actividad física, nutrición, toxicidad, alcohol, sueño y estrés, tipo de personalidad y actividades, imagen interior, control de la salud y sexualidad y orden-, los puntajes obtenidos en el Alpha de Cronbach fueron: pertinencia: 0,932; relevancia: 0,904; utilidad: 0,948; redacción: 0,846 y claridad 0,800. Conclusiones: El cuestionario FANTASTICO se adapta como una herramienta breve y útil para la aproximación a los estilos de vida de adolescentes escolarizados desde un enfoque holístico. Es un instrumento fiable para esta población.

Objective: To carry out validation and content adaptation of the "FANTASTIC" questionnaire trough the Delphi technique to characterize lifestyles among adolescent students. Materials and methods: Validations and adaptation studies with 12 experts, which used consensus Delphi technique, into three rounds; an Cronbach’s Alpha > 0,80 was considered as an optimum value of pertinence, worthiness, relevance, usefulness, writing composition, and clarity. Results: The 30 items included in the questionnaire test behaviors on ten dimensions of life style, as: relatives, friends, associate content, physical activity, nutrishment, toxicity, alcohol, sleepiness, stress, personality sort, and activities, inner image, health control, sexuality, and order. The scores obtained from the Cronbach’s Alpha were: pertinence 0,932; relevance 0,904; usefulness 0,948; writing composition 0,846 and clearness 0,800. Conclusions: The "FANTASTIC" questionnaire can be adapted like a useful and brief tool to approach the schooled adolescents’ lifestyle from a holistic focus. It is a reliable instrument to study this population.