Evaluation of liver regeneration with use of diet supplemented with L-arginine.

PURPOSE: To compare controlled liver regeneration in rats submitted to 60% hepatic resection having L-arginine supplemented diet, based on weight changes of the regenerated liver, laboratory parameters of liver function and pathological findings. METHODS: Thirty-six rats were divided into two groups, control and L- arginine. The first received standard chow and saline solution by gavage. The second had supplementation with L- arginine. Animals were killed on postoperative period at 24h, 72h and seven days. For analysis of liver regeneration was used Kwon formula for weight, laboratory tests and mitosis. RESULTS: Weight, showed no benefit with L- arginine supplementation; however, intergroup comparison in the first 24h observed positive effect on supplementation (p=0.008). Alkaline phosphatase was increased in arginine group (p<0.04). The number of mitoses showed no difference between the two groups; however, in the first 24 hours, the supplemented group had higher number of mitoses within the groups (p=0.03). CONCLUSION: Supplementation with L-arginine did not show benefits in liver regeneration; however, supplemented group in the first 24 hours showed benefits over 72 hours and seven days of the evaluation by weight gain and number of mitosis.

Evaluation of liver regeneration with use of diet supplemented with l-arginine

PURPOSE: To compare controlled liver regeneration in rats submitted to 60% hepatic resection having L-arginine supplemented diet, based on weight changes of the regenerated liver, laboratory parameters of liver function and pathological findings. METHODS: Thirty-six rats were divided into two groups, control and L- arginine. The first received standard chow and saline solution by gavage. The second had supplementation with L- arginine. Animals were killed on postoperative period at 24h, 72h and seven days. For analysis of liver regeneration was used Kwon formula for weight, laboratory tests and mitosis. RESULTS: Weight, showed no benefit with L- arginine supplementation; however, intergroup comparison in the first 24h observed positive effect on supplementation (p=0.008). Alkaline phosphatase was increased in arginine group (p<0.04). The number of mitoses showed no difference between the two groups; however, in the first 24 hours, the supplemented group had higher number of mitoses within the groups (p=0.03). CONCLUSION: Supplementation with L-arginine did not show benefits in liver regeneration; however, supplemented group in the first 24 hours showed benefits over 72 hours and seven days of the evaluation by weight gain and number of mitosis. .

Comparison between electrocautery and fibrin selant after hepatectomy in rats.

OBJECTIVE: To compare between electrocautery and fibrin sealant hemostasis in rats after partial hepatectomy. METHODS: we used 24 Wistar rats, which were submitted to 30% hepatic resection, divided into two groups of 12 animals each: Group Electrocautery and Group Tachosil(r). These animals were evaluated after three and 14 days. We assessed the presence of complications, laboratory tests and histological exam of the recovered liver. RESULTS: the presence of abscess was more prevalent in the electrocautery group. The observed adhesions were more pronounced in the electrocautery group, both in frequency and in intensity, after three and 14 days. There were no deaths in either group. As for laboratory analysis, after three days the hematocrit was lower in the TachoSil(r) Group. The elevation of AST and ALT were more pronounced in the electrocautery group (p = 0.002 and p = 0.004) in three days. Histological analysis of specimens collected on the third day after surgery showed similar results in both groups for the presence of polymorphonuclear cells, whereas mononuclear was more evident in the TachoSil(r) group. We also observed that angiogenesis, although present in both groups, was more pronounced in the TachoSil(r) group (p = 0.030). However, on the 14th day angiogenesis was more pronounced in the electrocautery group, but without statistical significance. CONCLUSION: hemostasis achieved by the groups was similar; however, the use of electrocautery was associated with infections, adhesions at higher grades and elevated liver enzymes.

Comparison between electrocautery and fibrin selant after hepatectomy in rats / Estudo comparativo entre eletrocautério e selante de fibrina após hepatectomia em ratos

OBJECTIVE: To compare between electrocautery and fibrin sealant hemostasis in rats after partial hepatectomy. METHODS: we used 24 Wistar rats, which were submitted to 30% hepatic resection, divided into two groups of 12 animals each: Group Electrocautery and Group Tachosil(r). These animals were evaluated after three and 14 days. We assessed the presence of complications, laboratory tests and histological exam of the recovered liver. RESULTS: the presence of abscess was more prevalent in the electrocautery group. The observed adhesions were more pronounced in the electrocautery group, both in frequency and in intensity, after three and 14 days. There were no deaths in either group. As for laboratory analysis, after three days the hematocrit was lower in the TachoSil(r) Group. The elevation of AST and ALT were more pronounced in the electrocautery group (p = 0.002 and p = 0.004) in three days. Histological analysis of specimens collected on the third day after surgery showed similar results in both groups for the presence of polymorphonuclear cells, whereas mononuclear was more evident in the TachoSil(r) group. We also observed that angiogenesis, although present in both groups, was more pronounced in the TachoSil(r) group (p = 0.030). However, on the 14th day angiogenesis was more pronounced in the electrocautery group, but without statistical significance. CONCLUSION: hemostasis achieved by the groups was similar; however, the use of electrocautery was associated with infections, adhesions at higher grades and elevated liver enzymes. .

OBJETIVO: comparar a hemostasia entre eletrocautério e selante de fibrina em ratos submetidos à hepatectomia. MÉTODOS: foram utilizados 24 ratos Wistar submetidos à ressecção hepática de 30%, divididos em dois grupos contendo 12 animais cada: Grupo Eletrocautério e Grupo TachoSil(r). Estes animais foram aferidos após três e 14 dias. Avaliou-se presença de complicações, exames laboratoriais e estudo histológico do fígado recuperado. RESULTADOS: presença de abscesso foi mais prevalente no Grupo Eletrocautério. As aderências foram observadas mais pronunciadas no Grupo Eletrocautério tanto em frequência e intensidade, após três e 14 dias. Não houve óbito em ambos os grupos. Na análise laboratorial, comparando-se Grupo Eletrocautério e Grupo TachoSil(r), após três dias o hematócrito foi menor no Grupo TachoSil(r). A elevação das enzimas AST e ALT foram mais pronunciadas no Grupo Eletrocautério (p=0,002 e p=0,004) em três dias. Na análise histológica, no terceiro dia do pós-operatório, resultado semelhante foi encontrado nos dois grupos quanto à presença de polimorfonucleares, enquanto que mononucleares foi mais evidente no TachoSil(r). Observou-se ainda que a angiogênese, embora presente nos dois grupos, foi mais acentuada no Grupo TachoSil(r) (p=0,030). Entretanto, no 14º dia, a angiogênese foi mais pronunciada no Grupo Eletrocautério, mas sem significância estatística. . CONCLUSÃO: a hemostasia alcançada pelos grupos foi semelhante; no entanto, o uso do eletrocautério esteve associado à infecções, aderências abdominais de graus mais elevados e à elevação das enzimas hepáticas. .