RESUMO
Cytochrome bd-type quinol oxidases catalyze the reduction of molecular oxygen to water in the respiratory chain of many human-pathogenic bacteria. They are structurally unrelated to mitochondrial cytochrome c oxidases and are therefore a prime target for the development of antimicrobial drugs. We determined the structure of the Escherichia coli cytochrome bd-I oxidase by single-particle cryo-electron microscopy to a resolution of 2.7 angstroms. Our structure contains a previously unknown accessory subunit CydH, the L-subfamily-specific Q-loop domain, a structural ubiquinone-8 cofactor, an active-site density interpreted as dioxygen, distinct water-filled proton channels, and an oxygen-conducting pathway. Comparison with another cytochrome bd oxidase reveals structural divergence in the family, including rearrangement of high-spin hemes and conformational adaption of a transmembrane helix to generate a distinct oxygen-binding site.
Assuntos
Grupo dos Citocromos b/química , Complexo de Proteínas da Cadeia de Transporte de Elétrons/química , Proteínas de Escherichia coli/química , Escherichia coli/enzimologia , Oxirredutases/química , Domínio Catalítico , Microscopia Crioeletrônica , Heme/química , Modelos Moleculares , Oxirredução , Oxigênio/química , Estrutura Quaternária de Proteína , Subunidades Proteicas/química , Prótons , Ubiquinona/químicaRESUMO
We report the implementation of an electrostatic Einzel lens (Boersch) phase plate in a prototype transmission electron microscope dedicated to aberration-corrected cryo-EM. The combination of phase plate, C(s) corrector and Diffraction Magnification Unit (DMU) as a new electron-optical element ensures minimal information loss due to obstruction by the phase plate and enables in-focus phase contrast imaging of large macromolecular assemblies. As no defocussing is necessary and the spherical aberration is corrected, maximal, non-oscillating phase contrast transfer can be achieved up to the information limit of the instrument. A microchip produced by a scalable micro-fabrication process has 10 phase plates, which are positioned in a conjugate, magnified diffraction plane generated by the DMU. Phase plates remained fully functional for weeks or months. The large distance between phase plate and the cryo sample permits the use of an effective anti-contaminator, resulting in ice contamination rates of <0.6 nm/h at the specimen. Maximal in-focus phase contrast was obtained by applying voltages between 80 and 700 mV to the phase plate electrode. The phase plate allows for in-focus imaging of biological objects with a signal-to-noise of 5-10 at a resolution of 2-3 nm, as demonstrated for frozen-hydrated virus particles and purple membrane at liquid-nitrogen temperature.
Assuntos
Microscopia Crioeletrônica/instrumentação , Microscopia Crioeletrônica/métodos , Substâncias Macromoleculares/análise , Membrana Purpúrea/ultraestrutura , Vírus do Mosaico do Tabaco/ultraestrutura , Crioultramicrotomia , Congelamento , Halobacterium salinarum/ultraestrutura , Microscopia Eletrônica de Transmissão/instrumentação , Microscopia Eletrônica de Transmissão/métodos , Eletricidade EstáticaRESUMO
Amphipols (APols) are short amphipathic polymers that can substitute for detergents to keep integral membrane proteins (MPs) water soluble. In this review, we discuss their structure and solution behavior; the way they associate with MPs; and the structure, dynamics, and solution properties of the resulting complexes. All MPs tested to date form water-soluble complexes with APols, and their biochemical stability is in general greatly improved compared with MPs in detergent solutions. The functionality and ligand-binding properties of APol-trapped MPs are reviewed, and the mechanisms by which APols stabilize MPs are discussed. Applications of APols include MP folding and cell-free synthesis, structural studies by NMR, electron microscopy and X-ray diffraction, APol-mediated immobilization of MPs onto solid supports, proteomics, delivery of MPs to preexisting membranes, and vaccine formulation.
Assuntos
Proteínas de Membrana/química , Proteínas de Membrana/ultraestrutura , Modelos Químicos , Modelos Moleculares , Polímeros/química , Sítios de Ligação , Simulação por Computador , Ligação ProteicaRESUMO
In this study, structural, functional, and mechanistic properties of the Na(+)/H(+) antiporter MjNhaP1 from Methanococcus jannaschii were analyzed by infrared spectroscopic techniques. Na(+)/H(+) antiporters are generally responsible for the regulation of cytoplasmic pH and Na(+) concentration. MjNhaP1 is active in the pH range between pH 6 and pH 6.5; below and above it is inactive. The secondary structure analysis on the basis of ATR-IR spectra provides the first insights into the structural changes between inactive (pH 8) and active (pH 6) state of MjNhaP1. It results in decreased ordered structural elements with increasing the pH-value i.e. with inactivation of the protein. Analysis of temperature-dependent FTIR spectra indicates that MjNhaP1 in the active state exhibits a much higher unfolding temperature in the spectral region assigned to alpha-helical segments. In contrast, the temperature-induced structural changes for beta-sheet structure are similar for inactive and active state. Consequently, this structure element is not the part of the activation region of the protein. The surface accessibility of the protein was analyzed by following the extent of H/D exchange. Due to higher content of unordered structural elements a higher accessibility for amide protons is observed for the inactive as compared to the active state of MjNhaP1. Altogether, the results present the active state of MjNhaP1 as the state with ordered structural elements which exhibit high thermal stability and increased hydrophobicity.
Assuntos
Proteínas Arqueais/química , Proteínas Arqueais/metabolismo , Mathanococcus/metabolismo , Trocadores de Sódio-Hidrogênio/química , Trocadores de Sódio-Hidrogênio/metabolismo , Óxido de Deutério , Concentração de Íons de Hidrogênio , Conformação Proteica , Estrutura Secundária de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier , TermodinâmicaRESUMO
The high-affinity glycine betaine uptake system BetP, an osmosensing and osmoregulated sodium-coupled symporter from Corynebacterium glutamicum, was overexpressed in Escherichia coli with an N-terminal StrepII-tag, solubilized in beta-dodecylmaltoside and purified by streptactin affinity chromatography. Analytical ultracentrifugation indicated that BetP forms trimers in detergent solution. Detergent-solubilized BetP can be reconstituted into proteoliposomes without loss of function, suggesting that BetP is a trimer in the bacterial membrane. Reconstitution with E.coli polar lipids produced 2D crystals with unit cell parameters of 182A x 154A, gamma=90 degrees exhibiting p22(1)2(1) symmetry. Electron cryo-microscopy yielded a projection map at 7.5A. The unit cell contains four non-crystallographic trimers of BetP. Within each monomer, ten to 12 density peaks characteristic of transmembrane alpha-helices surround low-density regions that define potential transport pathways. Small but significant differences between the three monomers indicate that the trimer does not have exact 3-fold symmetry. The observed differences may be due to crystal packing, or they may reflect different functional states of the transporter, related to osmosensing and osmoregulation. The projection map of BetP shows no clear resemblance to other secondary transporters of known structure.
Assuntos
Corynebacterium/química , Proteínas de Membrana Transportadoras/química , Simportadores/química , Marcadores de Afinidade , Clonagem Molecular/métodos , Microscopia Crioeletrônica , Cristalização , Lipossomos , Osmose , Conformação Proteica , Estrutura Quaternária de Proteína , Proteínas Recombinantes de Fusão/isolamento & purificaçãoRESUMO
Chlorophyll (Chl) molecules attached to plant light-harvesting complexes (LHC) differ in their spectral behavior. While most Chl a and Chl b molecules give rise to absorption bands between 645 nm and 670 nm, some special Chls absorb at wavelengths longer than 700 nm. Among the Chl a/b-antennae of higher plants these are found exclusively in LHC I. In order to assign this special spectral property to one chlorophyll species we reconstituted LHC of both photosystem I (Lhca4) and photosystem II (Lhcb1) with carotenoids and only Chl a or Chl b and analyzed the effect on pigment binding, absorption and fluorescence properties. In both LHCs the Chl-binding sites of the omitted Chl species were occupied by the other species resulting in a constant total number of Chls in these complexes. 77-K spectroscopic measurements demonstrated that omission of Chl b in refolded Lhca4 resulted in a loss of long-wavelength absorption and 730-nm fluorescence emission. In Lhcb1 with only Chl b long-wavelength emission was preserved. These results clearly demonstrate the involvement of Chl b in establishing long-wavelength properties.
Assuntos
Clorofila/metabolismo , Complexo de Proteínas do Centro de Reação Fotossintética/química , Complexo de Proteínas do Centro de Reação Fotossintética/metabolismo , Absorção , Sítios de Ligação , Centrifugação com Gradiente de Concentração , Clorofila/química , Clorofila A , Complexos de Proteínas Captadores de Luz , Solanum lycopersicum , Complexo de Proteína do Fotossistema I , Complexo de Proteína do Fotossistema II , Pigmentos Biológicos/análise , Pigmentos Biológicos/química , Folhas de Planta , Ligação Proteica , Espectrometria de Fluorescência , Spinacia oleracea , TemperaturaAssuntos
Clorofila/fisiologia , Complexo de Proteínas do Centro de Reação Fotossintética/química , Cristalografia por Raios X , Transporte de Elétrons , Complexos de Proteínas Captadores de Luz , Substâncias Macromoleculares , Complexo de Proteínas do Centro de Reação Fotossintética/metabolismo , Plastocianina/metabolismo , Conformação Proteica , Água/metabolismoRESUMO
P-type ATPases convert chemical energy into electrochemical gradients that are used to energize secondary active transport. Analysis of the structure and function of P-type ATPases has been limited by the lack of active recombinant ATPases in quantities suitable for crystallographic studies aiming at solving their three-dimensional structure. We have expressed Arabidopsis thaliana plasma membrane H+-ATPase isoform AHA2, equipped with a His(6)-tag, in the yeast Saccharomyces cerevisiae. The H+-ATPase could be purified both in the presence and in the absence of regulatory 14-3-3 protein depending on the presence of the diterpene fusicoccin which specifically induces formation of the H+-ATPase/14-3-3 protein complex. Amino acid analysis of the purified complex suggested a stoichiometry of two 14-3-3 proteins per H+-ATPase polypeptide. The purified H(+)-ATPase readily formed two-dimensional crystals following reconstitution into lipid vesicles. Electron cryo-microscopy of the crystals yielded a projection map at approximately 8 A resolution, the p22(1)2(1) symmetry of which suggests a dimeric protein complex. Three distinct regions of density of approximately equal size are apparent and may reflect different domains in individual molecules of AHA2.
Assuntos
Arabidopsis/enzimologia , Membrana Celular/enzimologia , Microscopia Crioeletrônica , ATPases Translocadoras de Prótons/química , ATPases Translocadoras de Prótons/isolamento & purificação , Proteínas 14-3-3 , Arabidopsis/genética , Cristalização , Dimerização , Glicosídeos/farmacologia , Isoenzimas/química , Isoenzimas/genética , Isoenzimas/isolamento & purificação , Isoenzimas/ultraestrutura , Lipossomos/química , Lipossomos/metabolismo , Ligação Proteica/efeitos dos fármacos , Estrutura Quaternária de Proteína , ATPases Translocadoras de Prótons/genética , ATPases Translocadoras de Prótons/metabolismo , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/isolamento & purificação , Proteínas Recombinantes de Fusão/metabolismo , Saccharomyces cerevisiae/genética , Relação Estrutura-Atividade , Tirosina 3-Mono-Oxigenase/química , Tirosina 3-Mono-Oxigenase/isolamento & purificação , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
Two-dimensional crystals of a membrane protein, the proton ATPase from plant plasma membranes, have been obtained by a new strategy based on the use of functionalized, fluorinated lipids spread at the air-water interface. Monolayers of the fluorinated lipids are stable even in the presence of high concentrations of various detergents as was established by ellipsometry measurements. A nickel functionalized fluorinated lipid was spread into a monolayer at the air-water interface. The overexpressed His-tagged ATPase solubilized by detergents was added to the subphase. 2D crystals of the membrane protein, embedded in a lipid bilayer, formed as the detergent was removed by adsorption. Electron microscopy indicated that the 2D crystals were single layers with dimensions of 10 microm or more. Image processing yielded a projection map at 9 A resolution, showing three well-separated domains of the membrane-embedded proton ATPase.
Assuntos
Microscopia Crioeletrônica , Detergentes/metabolismo , Metabolismo dos Lipídeos , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Membranas Artificiais , Adsorção , Ar , Arabidopsis/química , Arabidopsis/enzimologia , Quelantes/síntese química , Quelantes/química , Quelantes/metabolismo , Cristalização , Detergentes/farmacologia , Flúor/metabolismo , Processamento de Imagem Assistida por Computador , Bicamadas Lipídicas/química , Bicamadas Lipídicas/metabolismo , Lipídeos/síntese química , Lipídeos/química , Proteínas de Membrana/ultraestrutura , Micelas , Níquel/antagonistas & inibidores , Níquel/metabolismo , Pressão , Ligação Proteica , Estrutura Terciária de Proteína , ATPases Translocadoras de Prótons/química , ATPases Translocadoras de Prótons/metabolismo , ATPases Translocadoras de Prótons/ultraestrutura , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/metabolismo , Proteínas Recombinantes de Fusão/ultraestrutura , Solubilidade/efeitos dos fármacos , Água/metabolismoRESUMO
The major route for protein export or membrane integration in bacteria occurs via the Sec-dependent transport apparatus. The core complex in the inner membrane, consisting of SecYEG, forms a protein-conducting channel, while the ATPase SecA drives translocation of substrate across the membrane. The SecYEG complex from Escherichia coli was overexpressed, purified and crystallized in two dimensions. A 9 A projection structure was calculated using electron cryo-microscopy. The structure exhibits P12(1) symmetry, having two asymmetric units inverted with respect to one another in the unit cell. The map shows elements of secondary structure that appear to be transmembrane helices. The crystallized form of SecYEG is too small to comprise the translocation channel and does not contain a large pore seen in other studies. In detergent solution, the SecYEG complex displays an equilibrium between monomeric and tetrameric forms. Our results therefore indicate that, unlike other known channels, the SecYEG complex can exist as both an assembled channel and an unassembled smaller unit, suggesting that transitions between the two states occur during a functional cycle.
Assuntos
Proteínas de Bactérias/química , Proteínas de Escherichia coli , Oligopeptídeos/química , Peptidil Transferases/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Cristalização , Escherichia coli/enzimologia , Oligopeptídeos/genética , Oligopeptídeos/isolamento & purificação , Oligopeptídeos/metabolismo , Testes de Precipitina , Canais de Translocação SEC , SoluçõesRESUMO
The Escherichia coli porin OmpG, which acts as an efficient unspecific channel for mono-, di- and trisaccharides, has been purified and crystallized in two dimensions. Projection maps of two different crystal forms of OmpG at 6 A resolution show that the protein has a beta-barrel structure characteristic for outer membrane proteins, and that it does not form trimers, unlike most other porins such as OmpF and OmpC, but appears in monomeric form. The size of the barrel is approximately 2.5 nm, indicating that OmpG may consist of 14 beta-strands. The projection map suggests that the channel is restricted by internal loops.
Assuntos
Proteínas de Escherichia coli , Escherichia coli/química , Porinas/química , Porinas/ultraestrutura , Proteínas da Membrana Bacteriana Externa , Cristalização , Cristalografia , Elétrons , Análise de Fourier , Processamento de Imagem Assistida por Computador , Modelos Moleculares , Porinas/isolamento & purificação , Porinas/metabolismo , Estrutura Quaternária de Proteína , Estrutura Secundária de ProteínaRESUMO
GlpF, the glycerol facilitator protein of Escherichia coli, is an archetypal member of the aquaporin superfamily. To assess its structure, recombinant histidine-tagged protein was overexpressed, solubilized in octylglucoside and purified to homogeneity. Negative stain electron microscopy of solubilized GlpF protein revealed a tetrameric structure of approximately 80 A side length. Scanning transmission electron microscopy yielded a mass of 170 kDa, corroborating the tetrameric nature of GlpF. Reconstitution of GlpF in the presence of lipids produced highly ordered two-dimensional crystals, which diffracted electrons to 3.6 A resolution. Cryoelectron microscopy provided a 3.7 A projection map exhibiting a unit cell comprised of two tetramers. In projection, GlpF is similar to AQP1, the erythrocyte water channel. However, the major density minimum within each monomer is distinctly larger in GlpF than in AQP1.
Assuntos
Aquaporinas/química , Proteínas da Membrana Bacteriana Externa/química , Proteínas de Escherichia coli , Escherichia coli/química , Proteínas da Membrana Bacteriana Externa/ultraestrutura , Proteínas de Bactérias/química , Cristalização , Escherichia coli/metabolismo , Microscopia Eletrônica , Microscopia Eletrônica de VarreduraRESUMO
The three-dimensional structure of GlpF, the glycerol facilitator of Escherichia coli, was determined by cryo-electron microscopy. The 6.9-A density map calculated from images of two-dimensional crystals shows the GlpF helices to be similar to those of AQP1, the erythrocyte water channel. While the helix arrangement of GlpF does not reflect the larger pore diameter as seen in the projection map, additional peripheral densities observed in GlpF are compatible with the 31 additional residues in loops C and E, which accordingly do not interfere with the inner channel construction. Therefore, the atomic structure of AQP1 was used as a basis for homology modeling of the GlpF channel, which is predicted to be free of bends, wider, and more vertically oriented than the AQP1 channel. Furthermore, the residues facing the GlpF channel exhibit an amphiphilic nature, being hydrophobic on one side and hydrophilic on the other side. This property may partially explain the contradiction of glycerol diffusion but limited water permeation capacity.
Assuntos
Proteínas da Membrana Bacteriana Externa/química , Proteínas de Escherichia coli , Escherichia coli/química , Aquaporina 1 , Aquaporinas/química , Glicerol , Modelos Moleculares , Estrutura Molecular , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Homologia de Sequência de Aminoácidos , Especificidade por SubstratoRESUMO
Mutants of plant light-harvesting complex II (LHC-II) were produced by refolding the complex in vitro from bacterially expressed apoprotein and purified pigments by a method which yields native-like LHC-II in a single step. Amino acid residues known from the structure of the complex [Kühlbrandt, W., et al. (1994) Nature 367, 614-621] to bind chlorophyll (Chl) were replaced with nonbinding residues by site-directed mutagenesis. Recombinant monomeric and trimeric pigment-protein complexes were separated by density gradient centrifugation, and their pigment composition was determined. Six out of nine mutants formed trimers with Chl a:Chl b ratios and Chl contents which suggested they were lacking one Chl a or b per polypeptide. In this way, the identities of Chls a1, a2, a3, b5, and b6 were confirmed as Chl a or b, respectively, whereas Chl b3 in the structure was found to be a Chl a. Absorption and fluorescence emission spectra of the mutant lacking Chl a2 indicated a central role for this Chl in energy transfer to the reaction center.
Assuntos
Clorofila/genética , Clorofila/metabolismo , Complexo de Proteínas do Centro de Reação Fotossintética/química , Complexo de Proteínas do Centro de Reação Fotossintética/genética , Asparagina/genética , Sítios de Ligação/genética , Carotenoides/análise , Ácido Glutâmico/genética , Glutamina/genética , Complexos de Proteínas Captadores de Luz , Modelos Moleculares , Mutagênese Sítio-Dirigida , Pisum sativum , Complexo de Proteínas do Centro de Reação Fotossintética/isolamento & purificação , Complexo de Proteínas do Centro de Reação Fotossintética/metabolismo , Dobramento de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Espectrometria de Fluorescência , EspectrofotometriaRESUMO
The purification and two-dimensional crystallization of highly active cytochrome b(6)f complex from spinach is described. The preparation shows all spectroscopic characteristics of the pure complex. The electron transfer activity of 450+/-60 electrons per s is the highest in vitro activity reported to date. Using dimethyl sulfoxide (DMSO) as a solvent for the electron donor enhanced the performance and reproducibility of the assay. The high yield and the high activity of the protein make it an ideal candidate for biophysical and structural studies. Preliminary two-dimensional crystallization experiments yielded several different forms of two-dimensional and thin three-dimensional crystals, exhibiting varying degrees of order.
Assuntos
Grupo dos Citocromos b/química , Grupo dos Citocromos b/isolamento & purificação , Spinacia oleracea/química , Animais , Chlamydomonas reinhardtii/química , Cristalografia , Grupo dos Citocromos b/metabolismo , Complexo Citocromos b6f , Transporte de Elétrons , Microscopia Eletrônica , Espectrofotometria , Fatores de TempoRESUMO
Electron cryo-microscopy has yielded a wealth of detailed new information on structures of biological macromolecules ranging from alphabeta-tubulin at 3.7 A resolution to hepatitis B virus at 7.4 A resolution, as well as a number of membrane proteins at 6-8 A resolution. Much of this progress was made possible by recent advances in instrumentation and image processing techniques.
Assuntos
Microscopia Crioeletrônica/métodos , Animais , Cristalografia por Raios X/métodos , Vírus da Hepatite B/ultraestrutura , Humanos , Processamento de Imagem Assistida por Computador , Espectroscopia de Ressonância Magnética , Proteínas de Membrana/ultraestrutura , Modelos MolecularesRESUMO
Electron crystallography of photosystem II has revealed the location of important subunits and photoactive pigment molecules within this large membrane protein complex. It has also demonstrated a close evolutionary link among all types of photosynthetic reaction centres.
Assuntos
Complexo de Proteínas do Centro de Reação Fotossintética/química , Cristalografia , Evolução Molecular , Substâncias Macromoleculares , Fotossíntese , Complexo de Proteínas do Centro de Reação Fotossintética/efeitos da radiação , Complexo de Proteína do Fotossistema II , Conformação ProteicaAssuntos
Proteínas de Membrana , Animais , Proteínas de Bactérias/química , Proteínas de Bactérias/fisiologia , Proteínas de Bactérias/ultraestrutura , Bioquímica/métodos , Bioquímica/tendências , Cristalografia por Raios X , Humanos , Proteínas de Membrana/química , Proteínas de Membrana/fisiologia , Proteínas de Membrana/ultraestrutura , Microscopia Eletrônica , Proteínas de Plantas/química , Proteínas de Plantas/fisiologia , Proteínas de Plantas/ultraestrutura , Conformação ProteicaRESUMO
BACKGROUND: Structures have recently been solved at 8 A resolution for both Ca2+-ATPase from rabbit sarcoplasmic reticulum and H+-ATPase from Neurospora crassa. These cation pumps are two distantly related members of the family of P-type ATPases, which are thought to use similar mechanisms to generate ATP-dependent ion gradients across a variety of cellular membranes. We have undertaken a detailed comparison of the two structures in order to describe their similarities and differences as they bear on their mechanism of active transport. RESULTS: Our first important finding was that the arrangement of 10 transmembrane helices was remarkably similar in the two molecules. This structural homology strongly supports the notion that these pumps use the same basic mechanism to transport their respective ions. Despite this similarity in the membrane-spanning region, the cytoplasmic regions of the two molecules were very different, both in their disposition relative to the membrane and in the juxtaposition of their various subdomains. CONCLUSIONS: On the basis of the crystallization conditions, we propose that these two crystal structures represent different intermediates in the transport cycle, distinguished by whether cations are bound to their transport sites. Furthermore, we propose that the corresponding conformational change (E2 to E1 ) has two components: the first is an inclination of the main cytoplasmic mass by 20 degrees relative to the membrane-spanning domain; the second is a rearrangement of the domains comprising the cytoplasmic part of the molecules. Accordingly, we present a rough model for this important conformational change, which relays the effects of cation binding within the membrane-spanning domain to the nucleotide-binding site, thus initiating the transport cycle.