Improving the stability of insulin through effective chemical modifications: A Comprehensive review.

Insulin, an essential peptide hormone, conjointly regulates blood glucose levels by its receptor and it is used as vital drug to treat diabetes. This therapeutic hormone may undergo different chemical modifications during industrial processes, pharmaceutical formulation, and through its endogenous storage in the pancreatic ß-cells. Insulin is highly sensitive to environmental stresses and readily undergoes structural changes, being also able to unfold and aggregate in physiological conditions. Even; small changes altering the structural integrity of insulin may have significant impacts on its biological efficacy to its physiological and pharmacological activities. Insulin analogs have been engineered to achieve modified properties, such as improved stability, solubility, and pharmacokinetics, while preserving the molecular pharmacology of insulin. The casually or purposively strategies of chemical modifications of insulin occurred to improve its therapeutic and pharmaceutical properties. Knowing the effects of chemical modification, formation of aggregates, and nanoparticles on protein can be a new look at the production of protein analogues drugs and its application in living system. The project focused on effects of chemical modifications and nanoparticles on the structure, stability, aggregation and their results in effective drug delivery system, biological activity, and pharmacological properties of insulin. The future challenge in biotechnology and pharmacokinetic arises from the complexity of biopharmaceuticals, which are often molecular structures that require formulation and delivery strategies to ensure their efficacy and safety.

Anti-inflammatory, immunomodulatory and anti-oxidant effects of Ocimum basilicum L. and its main constituents: A review.

Ocimum basilicum L. (O. basilicum) is an ornamental and therapeutic plant with various pharmacological effects and medical applications. In this article, detailed information on the anti-oxidant, immunomodulatory, and anti-inflammatory properties of O. basilicum and its main constituents was provided. The literature survey of the different databases until the end of November 2021 was explored on the immunomodulatory, anti-inflammatory and anti-oxidant effects of the herb and its constituents. The plant and its constituents showed diverse pharmacological effects including immunomodulatory, anti-inflammatory and anti-oxidant properties by improving of the inflammatory mediators including interleukin (IL)-10, IL-4, tumor necrosis factor-alpha (TNF-α), interferon gamma (IFN-γ), nitric oxide (NO), serum levels of IFN-γ, IL10 and IL-4, Ig. G, Ig. M and phospholipase A2 (PLA2), immunoglobulin E (Ig. E), total protein (TP), oxidant and anti-oxidant markers. O. basilicum and its main constituents therefore, could be effective on the treatment of diseases associated with inflammation, immune dysregulation and oxidative stress. The present review article provides readers with organized information about the anti-oxidant, immunomodulatory, and anti-inflammatory properties of O. basilicum.

Synthesis, 99mTc-radiolabeling, and biodistribution of new cellulose nanocrystals from Dorema kopetdaghens.

Cellulose nanocrystals (CNCs) are known as nano-biomaterials that can be achieved from the different sources. The designated CNCs have been successfully fabricated from the roots of Dorema kopetdaghens (Dk) plant by sulphuric acid hydrolysis method. Structural analysis has been carried out by the means of XRD, FTIR, and TGA/DTG procedures. The XRD results have indicated that the crystalline structure of CNCs had been cellulose I with the crystallinity index of 83.20% and size of 4.95 nm. The FTIR spectra have shown that the resulting samples have been related to the cellulose species. The thermal properties of CNCs have exhibited a lower thermal stability in comparison to the untreated roots. It has been indicated by the morphological analyses of FESEM, TEM, and AFM that the nanoparticles had contained a spherical shape. Also, the cytotoxicity of CNCs against A549 cell line has not exhibited any cytotoxic effects. The analysis of labeling efficiency in regards to 99mTc-CNCs has been observed to be above 98%, while the biodistribution of radioactivity has displayed a high uptake by the kidneys and blood circulation. Therefore, it is possible to transform the low-cost by-product into a beneficial substance such as CNCs that can be utilized in bioimaging applications.