RESUMO
Flavonoids are nearly ubiquitous in plants and are recognized as the pigments responsible for the colors of leaves, especially in autumn. They are rich in seeds, citrus fruits, olive oil, tea, and red wine. They are low molecular weight compounds composed of a three-ring structure with various substitutions. This basic structure is shared by tocopherols (vitamin E). Flavonoids can be subdivided according to the presence of an oxy group at position 4, a double bond between carbon atoms 2 and 3, or a hydroxyl group in position 3 of the C (middle) ring. These characteristics appear to also be required for best activity, especially antioxidant and antiproliferative, in the systems studied. The particular hydroxylation pattern of the B ring of the flavonoles increases their activities, especially in inhibition of mast cell secretion. Certain plants and spices containing flavonoids have been used for thousands of years in traditional Eastern medicine. In spite of the voluminous literature available, however, Western medicine has not yet used flavonoids therapeutically, even though their safety record is exceptional. Suggestions are made where such possibilities may be worth pursuing.
Assuntos
Flavonoides/farmacologia , Cardiopatias/tratamento farmacológico , Inflamação/tratamento farmacológico , Neoplasias/tratamento farmacológico , Animais , Antioxidantes/farmacologia , Antivirais/farmacologia , Ácido Ascórbico/farmacologia , Plaquetas/efeitos dos fármacos , Doença das Coronárias/tratamento farmacológico , Citoproteção , Inibidores Enzimáticos/farmacologia , Flavonoides/metabolismo , Humanos , Sistema Imunitário/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Mastócitos/efeitos dos fármacos , Xenobióticos/metabolismoRESUMO
1. Flavonoids display a wide range of pharmacological properties including anti-inflammatory. Anti-mutagenic, anti-carcinogenic and anti-cancer effects. Here, we evaluated the effects of eight flavonoids on the tumour cell proliferation, cellular protein phosphorylation, and matrix metalloproteinase (MMPs) secretion. 2. Of the flavonoids examined, luteolin (Lu) and quercetin (Qu) were the two most potent agents, and significantly inhibited A431 cell proliferation with IC50 values of 19 and 21 micronM, respectively. 3. The epidermal growth factor (EGF) (10 nM) promoted growth of A431 cells (+25+/-4.6%) and mediated epidermal growth factor receptor (EGFR) tyrosine kinase activity and autophosphorylation of EGFR were inhibited by Lu and Qu. At concentration of 20 micronM, both Lu and Qu markedly decreased the levels of phosphorylation of A431 cellular proteins, including EGFR. 4. A431 cells treated with Lu or Qu exhibited protuberant cytoplasmic blebs and progressive shrinkage morphology. Lu and Qu also time-dependently induced the appearance of a ladder pattern of DNA fragmentation, and this effect was abolished by EGF treatment. 5. The addition of EGF only marginally diminished the inhibitory effect of luteolin and quercetin on the growth rate of A431 cells, treatment of cellular proteins with EGF and luteolin or quercetin greatly reduced protein phosphorylation, indicating Lu and Qu may act effectively to inhibit a wide range of protein kinases, including EGFR tyrosine kinase. 6. EGF increased the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9), while Lu and Qu appeared to suppress the secretion of these two MMPs in A431 cells. 7. Examination of the relationship between the chemical structure and inhibitory effects of eight flavonoids reveal that the double bond between C2 and C3 in ring C and the OH groups on C3' and C4' in ring B are critical for the biological activities. 8. This study demonstrates that the inhibitory effects of Lu and Qu, and the stimulatory effects of EGF, on tumour cell proliferation, cellular protein phosphorylation, and MMP secretion may be mediated at least partly through EGFR. This study supports the idea that Lu and Qu may have potential as anti-cancer and anti-metastasis agents.
Assuntos
Inibidores Enzimáticos/farmacologia , Receptores ErbB/biossíntese , Flavonoides/farmacologia , Proteínas Tirosina Quinases/antagonistas & inibidores , Quercetina/farmacologia , Autorradiografia , Western Blotting , Divisão Celular/efeitos dos fármacos , Fragmentação do DNA , Densitometria , Eletroforese em Gel de Poliacrilamida , Humanos , Luteolina , Metaloproteinases da Matriz/metabolismo , Metástase Neoplásica/patologia , Fosforilação , Fosfotirosina/metabolismo , Testes de Precipitina , Células Tumorais CultivadasAssuntos
Antioxidantes/farmacologia , Flavonoides/farmacologia , Sequestradores de Radicais Livres/farmacologia , Animais , Antioxidantes/química , Antioxidantes/uso terapêutico , Ácido Ascórbico/farmacologia , Doença das Coronárias/fisiopatologia , Doença das Coronárias/prevenção & controle , Interações Medicamentosas , Flavonoides/química , Flavonoides/uso terapêutico , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/uso terapêutico , Radicais Livres/metabolismo , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Estrutura Molecular , Isquemia Miocárdica/fisiopatologia , Isquemia Miocárdica/prevenção & controle , Reperfusão Miocárdica , Oxirredução , Fagócitos/efeitos dos fármacos , Fagócitos/metabolismo , Plantas , Espécies Reativas de Oxigênio/metabolismoRESUMO
We examined the effects of flavone and two polyhydroxylated plant flavonoids (quercetin and fisetin), either singly or in combination with ascorbic acid, on the growth of a human squamous cell carcinoma cell line (HTB 43) in vitro. Fisetin and quercetin significantly impaired cell growth in the presence of ascorbic acid. Exposure of cells to ascorbic acid (2 micrograms/ml) and 2 micrograms/ml of either fisetin or quercetin resulted in 61 and 45% inhibition of cell growth, respectively, in 72 h, while treatment with ascorbic acid alone had no effect on cellular proliferation. Flavone and ascorbic acid, either as single agents or in combination, exhibited no significant inhibition at any of the concentrations tested. The enhancement of the antiproliferative effect of the above flavonoids by ascorbic acid may be due to its ability to protect these compounds against oxidative degradation.
Assuntos
Adjuvantes Farmacêuticos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Ácido Ascórbico/farmacologia , Carcinoma de Células Escamosas/tratamento farmacológico , Flavonoides/farmacologia , Carcinoma de Células Escamosas/patologia , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Sinergismo Farmacológico , Humanos , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
We investigated the antiproliferative effect of two polyhydroxylated (quercetin and taxifolin) and two polymethoxylated (nobiletin and tangeretin) flavonoids against three cell lines in tissue culture. Tangeretin and nobiletin markedly inhibited the proliferation of a squamous cell carcinoma (HTB 43) and a gliosarcoma (9L) cell line at 2-8 micrograms/ml concentrations. Quercetin displayed no effect on 9L cell growth at these concentrations, while at 8 micrograms/ml it inhibited HTB 43 cell growth. Taxifolin slightly inhibited HTB 43 cell growth at 8 micrograms/ml, while moderately inhibiting HTB 43 cell growth at 2-8 micrograms/ml. The proliferation of a human lung fibroblast-like cell line (CCL 135) was relatively insensitive to low concentrations of the above flavonoids.
Assuntos
Carcinoma de Células Escamosas/tratamento farmacológico , Flavonas , Flavonoides/farmacologia , Glioma/tratamento farmacológico , Pulmão/efeitos dos fármacos , Carcinoma de Células Escamosas/patologia , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Embrião de Mamíferos/citologia , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Flavonóis , Glioma/patologia , Humanos , Pulmão/citologia , Extratos Vegetais/farmacologia , Quercetina/análogos & derivados , Quercetina/farmacologia , Células Tumorais CultivadasRESUMO
No doubt can remain that the flavonoids have profound effects on the function of immune and inflammatory cells as determined by a large number and variety of in vitro and some in vivo observations. That these ubiquitous dietary chemicals may have significant in vivo effects on homeostasis within the immune system and on the behavior of secondary cell systems comprising the inflammatory response seems highly likely but more work is required to strengthen this hypothesis. Ample evidence indicates that selected flavonoids, depending on structure, can affect (usually inhibit) secretory processes, mitogenesis, and cell-cell interactions including possible effects on adhesion molecule expression and function. The possible action of flavonoids on the function of cytoskeletal elements is suggested by their effects on secretory processes. Moreover, evidence indicates that certain flavonoids may affect gene expression and the elaboration and effects of cytokines and cytokine receptors. How all of these effects are mediated is not yet clear but one important mechanism may be the capacity of flavonoids to stimulate or inhibit protein phosphorylation and thereby regulate cell function. Perhaps the counterbalancing effect of cellular protein tyrosine phosphatases will also be found to be affected by flavonoids. Some flavonoid effects can certainly be attributed to their recognized antioxidant and radical scavenging properties. A potential mechanism of action that requires scrutiny, particularly in relation to enzyme inhibition, is the redox activity of appropriately configured flavonoids. Finally, in a number of cell systems it seems that resting cells are not affected significantly by flavonoids but once a cell becomes activated by a physiological stimulus a flavonoid-sensitive substance is generated and interaction of flavonoids with that substance dramatically alters the outcome of the activation process.
Assuntos
Linfócitos B/efeitos dos fármacos , Flavonoides/farmacologia , Macrófagos/efeitos dos fármacos , Linfócitos T/efeitos dos fármacos , Animais , Linfócitos B/imunologia , Plaquetas/efeitos dos fármacos , Eosinófilos/efeitos dos fármacos , Humanos , Células Matadoras Naturais/efeitos dos fármacos , Macrófagos/imunologia , Neutrófilos/efeitos dos fármacos , Linfócitos T/imunologiaRESUMO
We examined the effects of four plant flavonoids (quercetin, taxifolin, nobiletin and tangeretin) on the in vitro growth of a human squamous cell carcinoma cell line (HTB43). Cell cultures were treated with each flavonoid (2-8 micrograms/ml) for 3-7 days. Cell viability, as determined by counting cells, correlated well with that obtained from a colorimetric assay for cellular growth utilizing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. The polymethoxylated flavonoids, nobiletin and tangeretin, markedly inhibited cell growth at all concentrations tested on days 5 and 7. On day 3, the inhibition observed was 70-72% at 8 micrograms/ml, while on day 5, it ranged from 61-88% at 2-4 micrograms/ml. Quercetin and taxifolin exhibited no significant inhibition at any of the concentrations tested. This difference in activity may be due to the relatively greater membrane uptake of the polymethoxylated flavonoids since methoxylation of the phenolic groups decreases hydrophilicity of the flavonoid.
Assuntos
Antineoplásicos/farmacologia , Flavonas , Flavonoides/farmacologia , Quercetina/análogos & derivados , Quercetina/farmacologia , Carcinoma de Células Escamosas , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Citrus , Ensaios de Seleção de Medicamentos Antitumorais , Flavonóis , HumanosRESUMO
1. The in vitro metabolism of [3H]benzo[a]pyrene (BP) and [14C]benzo[a]pyrene-7,8-dihydrodiol (BP-7,8-diol) by liver of brown bullhead (Ictalurus nebulosus) was characterized, as was the formation and persistence of BP-DNA adducts in vivo. 2. Compared to rat liver microsomes, bullhead liver microsomes produced relatively larger amounts of BP-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of of BP-7,8-diol (predominantly the [-] enantiomer) and smaller amounts of BP-4,5-diol. 3. BP phase I metabolites were efficiently converted by freshly isolated bullhead hepatocytes to conjugates, predominantly glucuronides. 4. BP-7,8-diol was metabolized by hepatocytes 4-fold more rapidly than was BP and was converted to approximately equal amounts of glucuronides, glutathione conjugates and sulfates. 5. BP-DNA adducts formed in bullhead liver with a lag time of several days and maximum adduct formation at 25-30 days. The major adduct was anti-BPDE-deoxyguanosine.
Assuntos
Benzo(a)pireno/metabolismo , DNA/metabolismo , Ictaluridae/metabolismo , Poluentes Químicos da Água/metabolismo , Animais , Feminino , Inativação Metabólica/fisiologia , Masculino , Microssomos Hepáticos/metabolismo , RatosRESUMO
The formation and persistence of benzo[a]pyrene (BP)-DNA adducts in the liver of brown bullheads (Ictalurus nebulosus) treated with the hydrocarbon (20 mg/kg body wt, i.p.) was investigated using the 32P-postlabeling assay. The highest level of covalent binding of BP to liver DNA (188 fmol BP adducts/mg DNA) was observed 25-30 days following treatment. After 70 days, the adduct level in liver DNA had declined to approximately 26% of the maximum adduct level. One major BP-DNA adduct and several minor ones were detected in the liver. The major adduct co-chromatographed with anti-BP-7,8-diol-9,10-epoxide-deoxyguanosine (anti-BPDE-dGuo) adduct. The data suggest that brown bullheads metabolically activate BP by the same mechanism as the mammalian systems susceptible to carcinogenic effects of the hydrocarbon.
Assuntos
Benzo(a)pireno/metabolismo , Peixes-Gato/metabolismo , Adutos de DNA , DNA/metabolismo , Ictaluridae/metabolismo , Fígado/metabolismo , 7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido/análise , Animais , Biotransformação , DNA/análiseRESUMO
Quercetin exhibits antitumor activity. We investigated the effect of quercetin on the in vitro and in vivo growth of two squamous cell carcinoma cell lines and a normal human lung fibroblast-like cell line. The in vivo effect was evaluated using implantable cell growth chambers implanted subcutaneously in immunocompetent rats. Quercetin was injected intraperitoneally, and multiple dosages were tested. Cells were counted on days 1, 3, 5, and 7, and growth curves were constructed. Quercetin caused inhibition of growth in both squamous cell carcinoma lines. Effect on the fibroblast-like human lung cells was noted only at the maximum concentration. Significant growth inhibition of squamous cell carcinoma was observed in implantable cell growth chambers retrieved 3 days after quercetin treatment. Quercetin appears to possess a cytotoxic effect on squamous cell carcinoma of head and neck origin both in vivo and in vitro. The inhibitory effect on malignant cells appears to be selective and dose-dependent.
Assuntos
Carcinoma de Células Escamosas/tratamento farmacológico , Flavonoides/farmacologia , Inibidores do Crescimento/farmacologia , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Quercetina/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Linhagem Celular , Cultura em Câmaras de Difusão , Relação Dose-Resposta a Droga , Fibroblastos/efeitos dos fármacos , Humanos , Masculino , Quercetina/administração & dosagem , Ratos , Ratos Endogâmicos , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
1. Liver microsomes from rats were considerably more active in metabolizing benzo[f]quinoline (B f Q) than those from brown bullheads (Ictalurus nebulosus). 2. The main B f Q metabolites formed by both rat and brown bullhead liver microsomes were qualitatively similar and included B f Q-7,8-dihydrodiol, B f Q-9,10-dihydrodiol, B f Q-N-oxide, 7-hydroxy B f Q, and 9-hydroxy B f Q. 3. The liver microsomes from control brown bullheads and rats metabolized B f Q primarily at the 7,8-and 9,10-positions, respectively, whereas in the case of microsomes from 3-methylcholanthrene (3-MC)-treated rats or brown bullheads, the major site of metabolic attack was the 7,8-position. 4. A 3-MC-type of cytochrome P-450 appears to be primarily responsible for the oxidation of B f Q by control brown bullhead liver microsomes, whereas a phenobarbital-inducible type of cytochrome P-450 seems to be involved in the metabolism of B f Q by control rat liver microsomes.
Assuntos
Peixes-Gato/metabolismo , Ictaluridae/metabolismo , Microssomos Hepáticos/metabolismo , Mutagênicos/metabolismo , Quinolinas/metabolismo , Ratos/metabolismo , AnimaisRESUMO
The formation of hepatic DNA adducts in male Sprague-Dawley rats following i.p. administration of benzo[f]quinoline (BfQ) was examined using a 32P-post-labeling assay. BfQ exhibited a low binding (11-27 amol adducts/microgram DNA) to liver DNA. Two BfQ-nucleoside adducts (one major and one minor) were detected. The BfQ-DNA adducts formed in vivo were chromatographically distinct from the adducts formed by the reaction of calf thymus DNA in vitro with BfQ-5,6-oxide, syn-7 beta,8 alpha-dihydroxy-9 beta,10 beta-epoxy-7,8,9,10-tetrahydroBfQ, anti-9 alpha,10 beta-dihydroxy-7 alpha,8 alpha-epoxy-7,8,9,10-tetrahydroBfQ, or anti-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydroBfQ-N- oxide. These results suggest that the bay-region diol epoxide of BfQ, unlike the bay-region diol epoxide derivatives of polynuclear aromatic hydrocarbons, is not involved in the covalent binding of BfQ to DNA.
Assuntos
DNA/metabolismo , Fígado/metabolismo , Quinolinas/farmacologia , Animais , Fígado/efeitos dos fármacos , Masculino , Ratos , Ratos EndogâmicosRESUMO
The metabolism of benzo[f]quinoline (BfQ) and its carbon analog phenanthrene has been compared in incubations with liver microsomes from control, 3-methylcholanthrene (3-MC)- and phenobarbital (PB)-pretreated rats. The rates of phenanthrene metabolism by the three types of microsomes were 0.7, 4.1 and 1.5 nmol/mg protein per min, respectively; the values for BfQ were 0.5, 3.7 and 2.5, respectively. Besides N-oxidation, the metabolism of BfQ by all the above microsomes was almost exclusively at the benzo-ring (49-69%) while that of phenanthrene was predominantly at the K-region (50-71%). Phenanthrene-1,2-dihydrodiol, a precursor of the bay-region diol epoxide of phenanthrene, was produced many times more than phenanthrene-3,4-dihydrodiol by both 3-MC- and PB-induced microsomes. While BfQ-7,8-dihydrodiol, the precursor of the bay-region diol epoxide of BfQ, was the predominant metabolite with 3-MC-induced microsomes, it was a minor metabolite with PB-induced microsomes. The benzo-ring oxidation of BfQ, but not of phenanthrene, was position-specific, i.e. predominantly 7,8-oxidation by 3-MC-induced microsomes and 9,10-oxidation by PB-induced microsomes, and implies that aza-substitution results in a site-specific attack by different cytochromes P-450.
Assuntos
Microssomos Hepáticos/metabolismo , Fenantrenos/metabolismo , Quinolinas/metabolismo , Animais , Cromatografia Líquida de Alta Pressão , Masculino , Metilcolantreno , Fenobarbital , Ratos , Ratos EndogâmicosRESUMO
Lipid peroxidation induced in rat alveolar macrophages incubated with chrysotile asbestos fibres was assessed by measuring the production of malondialdehyde. The rapid onset of lipid peroxidation in macrophages caused by these fibres suggests the process occurs during the attachment of the fibres on the macrophage membranes or during the very early steps of phagocytosis. Metal-ion chelators such as diethylenetriaminepentaacetic acid, 1,10-phenantroline and ethylenediaminetetraacetic acid, as well as iron-complexing agents such as bathophenantrolinesulphonate and desferrioxamine were found to substantially inhibit chrysotile-induced lipid peroxidation. These results suggest a possible role for the iron present in the asbestos structure in catalysing lipid peroxidation in alveolar macrophages. These observations also indicate that asbestos-catalysed lipid peroxidation may be one of the factors involved in asbestos-induced cell damage.
RESUMO
The metabolism of [1,3-14C]benzo[f]quinoline (BfQ) by liver microsomes from control, 3-methylcholanthrene (3-MC)-pretreated and phenobarbital (PB)-pretreated rats has been investigated in order to gain insights into the effect of mixed function oxidase inducers on the types and levels of specific metabolites as formed in vitro. The rates of metabolism of BfQ by liver microsomes from control, 3-MC- and PB-pretreated rats were 0.5, 3.6 and 2.4 nmol/min/mg of respectively. The most predominant metabolite of BfQ detected with liver microsomes from 3-MC-pretreated rats was BfQ-7,8-dihydrodiol, a precursor of the bay-region diol epoxide, constituting 41% of the total ethyl acetate-extractable metabolites. Other metabolites obtained along with their relative proportions were as follows: BfQ-N-oxide, 23% 7-hydroxyBfQ, 15%; 9-hydroxyBfQ, 9%; and BfQ-9,10-dihydrodiol, 6%. BfQ-5,6-dihydrodiol, a K-region dihydrodiol, was a trace metabolite representing approximately 1.0% of the total metabolism. Liver microsomes from PB-pretreated rats oxidized BfQ primarily to BfQ-N-oxide and 9-hydroxyBfQ, which constituted 41% and 20% of the total ethyl acetate-extractable metabolites of BfQ. The relative proportions of BfQ-9,10-dihydrodiol, BfQ-7,8-dihydrodiol and 7-hydroxy-BfQ formed were 12%, 3% and 13% respectively, while the figure for BfQ-5,6-dihydrodiol was 0.5%. The profile of metabolites formed by liver microsomes from control rats was similar to that generated by microsomes from PB-pretreated rats. While benzo-ring metabolites represented a major part of the metabolism of BfQ by liver microsomes from either 3-MC- or PB-pretreated rats, these two types of microsomes exhibited a positional selectivity in the oxidation of BfQ, the former primarily attacking the 7,8-position of BfQ while the latter preferentially oxidizing the 9,10-position. The preponderance of the potentially mutagenic BfQ-7,8-dihydrodiol amongst the metabolites generated by liver microsomes from 3-MC-pretreated rats suggests a possible role for cytochrome P-450c, the major form of rat hepatic cytochrome P-450 induced by 3-MC, in the metabolic activation of BfQ.
Assuntos
Quinolinas/farmacocinética , Animais , Indução Enzimática , Cinética , Masculino , Metilcolantreno/farmacologia , Microssomos Hepáticos/enzimologia , Oxigenases de Função Mista/biossíntese , Fenobarbital/farmacologia , Ratos , Ratos EndogâmicosRESUMO
Chrysotile asbestos fibers impair the activities of rat liver microsomal aryl hydrocarbon hydroxylase (AHH), aminopyrine (AP) N-demethylase and dimethylnitrosamine (DMN) demethylase in vitro. This inhibition is concentration-dependent. Preincubation of 3-methylcholanthrene (3-MC)-pretreated rat liver microsomes with chrysotile depresses the overall metabolism of [G-3H]benzo[a]pyrene (BaP). Various forms of asbestos employed inhibit AHH activity to the same extent. However, other types of asbestos are not as effective as chrysotile in diminishing AP demethylase activity. Chrysotile and crocidolite fibers are not found to significantly change the apparent Km of AHH activity, from 3-MC-pretreated rat liver microsomes, for BaP. Increasing the microsomal protein concentration partially abolishes the inhibition of AHH activity caused by chrysotile fibers. Inhibition of AP demethylase and AHH activities is attenuated by bovine serum albumin (BSA) or ferritin. Depression of AHH activity by crocidolite is significantly reversed by ferritin. Since polymers such as ferritin override enzyme inhibition by chrysotile as well as crocidolite, surface chemical groups of the fibers may be involved in enzyme modification.
Assuntos
Aminopirina N-Desmetilase/antagonistas & inibidores , Hidrocarboneto de Aril Hidroxilases/antagonistas & inibidores , Amianto/toxicidade , Microssomos Hepáticos/enzimologia , Animais , Benzo(a)pireno/metabolismo , Técnicas In Vitro , Masculino , Metilcolantreno/farmacologia , Microssomos Hepáticos/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Relação Estrutura-Atividade , Ácido p-Aminoipúrico/metabolismoRESUMO
The effects of chrysotile, water-leached chrysotile, and silica on microsomal metabolism of benzo(a)pyrene in vitro were studied. Examination of benzo(a)pyrene metabolites generated by 3-methylcholanthrene-pretreated rat liver microsomes, in the presence of chrysotile fibers, revealed a reduction in the overall metabolism of the hydrocarbon. Thus, chrysotile appeared to modify the activities of aryl hydrocarbon hydroxylase and epoxide hydrolase. Leaching chrysotile in deionized water for 24 hr elicited a similar response. Silica, in contrast to chrysotile, did not decrease the microsomal metabolism of benzo(a)pyrene. Chrysotile, as well as water-leached chrysotile, considerably diminished the microsomal production of water-soluble benzo(a)pyrene metabolites. Precoating the fibers with heparin or bovine serum albumin partially abolished this inhibition. The liver microsomal metabolism of benzo(a)pyrene in rats subjected to intraperitoneal administration of chrysotile was 58% of that in untreated rats.
Assuntos
Amianto/toxicidade , Benzopirenos/metabolismo , Microssomos Hepáticos/metabolismo , Dióxido de Silício/toxicidade , Animais , Asbestos Serpentinas , Benzo(a)pireno , Benzopireno Hidroxilase/metabolismo , Masculino , Microssomos Hepáticos/efeitos dos fármacos , Ratos , Ratos EndogâmicosAssuntos
Amianto/farmacologia , Benzopirenos/metabolismo , Membranas Intracelulares/metabolismo , Microssomos Hepáticos/enzimologia , Animais , Hidrocarboneto de Aril Hidroxilases/antagonistas & inibidores , Transporte Biológico/efeitos dos fármacos , Membranas Intracelulares/efeitos dos fármacos , Cinética , Micelas , Microssomos Hepáticos/efeitos dos fármacos , Fosfatidilcolinas/farmacologia , Coelhos , RatosRESUMO
A marked decrease in liver mitochondrial monoamine oxidase activity was noticed in rats fed a fat-free diet as compared with that of their controls. In lipid-deprived rats, the specific activity of this enzyme was very low towards different substrates studied. The activity of kynurenine 3-monooxygenase, which like monoamine oxidase is localized on the mitochondrial outer membrane, was similarly depressed under conditions of lipid deprivation. On the other hand no major changes were observed in the activity of the inner membrane enzyme, kynurenine amino-transferase. Mitochondria from fat-free diet-fed rats were deficient in essential fatty acids whereas no appreciable variations were found in the relative proportions of phospholipids in comparison with those of control mitochondria. Mitochondrial monoamine oxidase activity of the deficient rats retained its sensitivities to inhibitor drugs like clorgyline and deprenyl. No changes were noticeable in the substrate specificity of monoamine oxidase in these rats. When we switched the fat-free diet-fed rats to a diet supplemented with a source of essential fatty acids, there was an elevation in the activities of both monoamine oxidase and kynurenine 3-monooxygenase, their levels approaching those of the control rats.