RESUMO
OBJECTIVES: To study the changes in biochemical metabolites in the thalamus and the cerebellum and their association with clinical features in children with autism spectrum disorder (ASD). METHODS: In this prospective study, magnetic resonance spectroscopy (MRS) with point-resolved spatial selection was used to analyze the thalamus and the cerebellum at both sides in 50 children with ASD aged 2-6 years. Creatine (Cr) was as the internal standard to measure the relative values of N-acetylaspartate (NAA)/Cr, choline (Cho)/Cr, myoinositol (MI)/Cr, and glutamine and glutamate complex (Glx)/Cr, and the differences in metabolites and their association with clinical symptoms were compared. RESULTS: In the children with ASD, NAA/Cr in the left thalamus was positively correlated with the scores of hearing-language and hand-eye coordination in the Griffiths Development Scales-Chinese (P<0.05). Cho/Cr in the right cerebellum was positively correlated with the scores of personal-social competence, hearing-language, and hand-eye coordination (P<0.05). NAA/Cr and Glx/Cr in the left thalamus were positively correlated with those in the left cerebellum (P<0.05). There was no significant difference in metabolites between the left and right sides of the thalamus and the cerebellum in the children with ASD (P>0.05). CONCLUSIONS: There are metabolic disorders in the cerebellum and the thalamus in children with ASD, and there is a correlation between the changes of metabolites in the left cerebellum and the left thalamus. Some metabolic indexes are related to the clinical symptoms of ASD. MRS may reveal the pathological basis of ASD and provide a basis for diagnosis and prognosis assessment of ASD as a noninvasive and quantitative detection method.
Assuntos
Transtorno do Espectro Autista , Transtorno do Espectro Autista/diagnóstico por imagem , Cerebelo/diagnóstico por imagem , Criança , Colina , Humanos , Espectroscopia de Ressonância Magnética , Estudos Prospectivos , Tálamo/diagnóstico por imagemRESUMO
Normal Raman spectroscopy and surface-enhanced Raman spectroscopy of aspirin and aspirin tablet were reported, and the vibrational and enhanced peaks were assigned; the interaction of aspirin with DNA was investigated by SERS and UV. The results showed that NRS and SERS of aspirin and aspirin tablet were consistent basically, which indicated that excipient hardly affected the detection of aspirin; in SERS, aspirin was absorbed perpendicularly on silver colloid through the carboxyl group and the benzene ring; The interaction was mainly caused by the inserting-action mode between aspirin and DNA, and the benzene ring and C=O of aspirin were inserted between the base pair of the double helix structure of DNA, which provided important information and useful reference for understanding deeply the mechanism of action of this kind of drug.
Assuntos
Aspirina/química , DNA/química , Espectrofotometria Ultravioleta , Análise Espectral RamanRESUMO
Abstract Infrared spectroscopy (FTIR), normal Raman spectroscopy (NRS) of the solids and saturated solutions and surface-enhanced Raman spectroscopy (SERS) on the silver colloidal substrate were reported. The vibrational modes of each group were analyzed, and the enhanced peaks were assigned and compared. The results showed that the major differences between the epimers were the frequency of the CH3 asymmetric stretching vibration and symmetric in-plane deformation vibration of them. According to the mechanism and laws of SERS, the epimers were absorbed on the silver colloid through the P-O bond, carboxyl group and the five-membered ring perpendicularly. The establishment of this adsorption model which realized the rapid identification and the discrimination between the epimers provided important information and useful reference of the adrenal cortical hormone drugs.
RESUMO
Infrared spectroscopy (IR) and normal Raman spectroscopy (NRS) of Sudan red(III) and Sudan red(IV), and surface -enhanced Raman spectroscopy(SERS) of them on Cu-Ag nanocomposite material were reported in the present paper. The density functional theory (DFT) calculation was applied to calculate IR and NRS on a B3LYP/6-311+G (d, p) level, and the theoretically calculated spectra were carefully compared with the obtained experimental spectra. The vibrational peaks were assigned by the software of Gauss view 5.0. Rapid identification of Sudan red was realized, which provided profitable basis for characteristics and quick test of Sudan dyes, and reliable detection method of food security.