[Methodical bases of the use of erythrocytic diagnostic agents as phagocytosis objects]. / Metodicheskie osnovy primeneniia éritrotsitarnykh diagnostikumov v kachestve ob"ektov fagotsitoza.

The error in assessment of the neutrophil phagocytic activity is 5.8%. This is attained by removal of conglomerations of phagocytosis objects and by ruling out the minimal variations in the duration of contact between the blood and foreign corpuscles and preparation of smears to be examined on the same slide. The content of up to 90% red cells does not influence the neutrophil phagocytic activity, and higher concentration reduces its level.

[New methodological approaches to the use of the PHA reaction in determining anti-Shigella antibodies]. / Novye metodicheskie podkhody k ispol'zovaniiu RPGA pri opredelenii protivoshigelleznykh antitel.

Pronounced correlation between the titers of hemagglutinating antibodies to different types of shigellae and other bacteria in healthy persons and in sick persons of control groups has been established. On the basis of this regularity a new approach to the use of the passive hemagglutination test in shigellosis, taking into account the ratio of antibody titers to different Shigella types, has been developed. This essentially increases the sensitivity and specificity of the test, thus making it possible to standardize its evaluation and permitting the effective diagnosis of shigellosis from the onset of the disease.

[A rapid specific reaction of the blood phagocytes to bacteria and the new possibilities for its diagnostic determination in infections]. / Ekstrennaia spetsificheskaia reaktsiia fagotsitov krovi na bakterii i novye vozmozhnosti ee diagnosticheskogo opredeleniia pri infektsiiakh.

Materials on the study of specific reaction of phagocytes, rapidly appearing after the contact of the body with soluble microbial substances, are presented. The study has established that of the reaction-inducing stimulus has a non-antigenic character and it acts directly on phagocytes. The method for the determination of phagocytic reaction by the ratio of the phagocytosis levels in the specific and control objects has been developed, which makes it possible to use a common diagnostic criterion for different infections and to diagnose them from the onset of the disease.

[Periodontal function and nonspecific resistance in the dynamics of experimental hypo- and hyperthyroidism]. / Sostoianie parodonta i nespetsificheskoi rezistentnosti v dinamike éksperimental'nogo gipo- i gipertireoza.

Morphologic study of white rat periodontal tissue and assessment of nonspecific resistance of the body by whole blood antibacterial activity, skin bactericidal activity, blood serum and mixed salivary lysozyme activity were carried out over the course of experimental hypo- and hyperthyrosis starting from day 5 to day 90. The detected pathologic changes in the periodontium were in line with the time course of nonspecific resistance of the body. In hypothyrosis an earlier depression of nonspecific resistance factors was parallelled by earlier and more marked inflammatory destructive processes in the rat periodontium as compared to that in hyperthyrosis.

[Changes in integral indicators of natural immunity in experimental hypo- and hyperthyroidism]. / Dinamika integral'nykh pokazatelei estestvennogo immuniteta pri éksperimental'nom gipo- i gipertireoze.

Two natural immunity integral indices-the skin bactericidal action and the blood antibacterial activity-were studied in experimental rat hypo- and hyperthyrosis by means of original radioimmunoassay. A decrease in the skin bactericidal action was seen hypothyrosis, but as whole, this test proved to be insufficiently sensitive. An alteration of the blood antibacterial activity in hypo- and hyperthyrosis is characterized by an initial increase in this activity, followed by its lowering. The prolonged phase of the blood antibacterial activity growth was found in hyperthyrosis, whereas in hypothyrosis the decreased phase of the above activity was seen. The pronounced changes in the natural immunity level precede clinical manifestations of the thyroid affection. Radioimmunoassay is recommended for studying the blood antibacterial activity as a sensitive and informative test, allowing one to judge of the antibacterial immunity state.

[Data to substantiate the early immunological diagnosis of dysentery]. / Materialy k obosnovaniiu rannei immunologicheskoi diagnostiki dizenterii.

The interaction of the whole blood from patients with dysentery and gastrointestinal diseases of non-dysenteric etiology, with the causative agents of dysentery, Sh. sonnei and Sh. flexneri, and saprophytic, staphylococci labeled with radioactive isotopes was studied in vitro. In dysentery an increase in the capacity of the blood for Shigella fixation was observed from the beginning of the disease. During the 1st week of the disease this reaction was strictly specific and accompanied by a decrease in the fixation of staphylococci, but later the reaction became relatively specific. An increase in Shigella fixation occurred considerably earlier than immune antibody formation, as revealed by the indirect hemagglutination test. This research substantiates the possibility of an earlier immunological diagnosis of dysentery as compared with the serological methods.