In vivo study of the influence of adenine and guanosine on the erythrocytes of ACD (pH 5.0 and 5.6) preserved blood.

Data are presented for percentage of recovery, survival time (T1/2) and mode of sequestration of erythrocytes from ACD [disodium citrate 95 mmol/l and glucose (C6H12O6 X H2O)] 152 mmol/l or ACD--adenine or adenine + guanosine (pH ranging from 5.0 to 5.6) preserved blood for 35 days at 4-8 degrees C (277-281 K). With the availability of guanosine in 0.25 mmol/l or 0.5 mmol/l final concentration in ACD + 0.25 or 0.5 mmol/l adenine preserved blood a positive effect can be exerted on erythrocyte 24 hrs recovery and survival time (T1/2). This effect is particularly evident when pH of the preservative solution is raised to 5.6. Final concentrations of 0.25 mmol/l adenine and guanosine in ACD preserved blood (whole or packed erythrocytes, pH 5.6, Hct. 0.73 or 0.61) are sufficient to ensure 35 days of storage at 4-8 degrees C (277-281 K).

Comparative study on the percentage of recovery, survival and mode of sequestration of fresh and frozen preserved (-196 degrees C) human platelets.

Platelet concentrates amounting to 142 X 10(9) +/- 11 X 10(9) cells are prepared by cytopheresis from one liter of blood. Ten of the concentrates are labelled with 51Cr and reinfused autologously. The other ten concentrates are frozen with a controlled rate freezer in a medium containing 10% DMSO and 5% glucose for 7 days at -196 degrees C. The fresh platelets have a 51Cr 24 hrs. recovery of 68 +/- 7% and a survival rate (T 1/2) of 10 +/- 1 days. The frozen preserved platelets have a 51Cr 24 hrs. recovery of 54 +/- 6.1%, a T 1/2 of 8.1 days, and significantly increased liver sequestration. There is a correlation between the reversal reaction in vitro after hypotonic shock and the 51Cr 24 hrs. in vivo recovery of fresh platelets and the 51Cr 1st hr. recovery of frozen platelets.

Assessment of the extent of bone marrow haemopoietic activity in normal subjects.

A method, introduced by the authors, for assessment of the extent of bone marrow haemopoietic activity in normal subjects is reported. 99mTc-sulfur colloid is injected, and the bone marrow visualized on gamma camera, type Phogamma-HP, which is equipped with 4096 channel analyzer (Nuclear Chicago). By use of a standard and by registering the activity in "zones of interest" from the arm, forearm, thigh and lower leg bones, indices and mean normal values in 26 normal subjects were calculated and reported.

[Quantitative changes in produced and destroyed erythrocytes in hemopathy]. / Promeni v kolichestvoto proizvedeni i razrusheni eritrotsiti pri niakoi khemopatii.

Ten healthy volunteers and 38 patients with iron deficiency anemia, aplastic anemia, chronic lymphadenosis, myelofibrosis were examined. On the base of the results from the kinetics of iron-59 and the marked with chromium-51 erythrocytes and from the corrected per cent reticulocytes, the authors set up the task to evaluate the etythropoetic potentialities of bone marrow and the severity of erythrocyte balance disorder, studying the quantity of produced and destroyed erythrocytes daily and comparing the data obtained with those from aspiration biopsy and treparobiopsy. The results, obtained by the authors, reveal the high potentialities indicated by the isotope investigation with iron-59 and chromium-51 as well as the corrected per cent of reticulocytes, for the evaluation of the erythropoetic potentialities of bone marrow and the high correlation between the results obtained and the clinical picture.