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1.
Mol Ecol ; 30(16): 3965-3973, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34145933

RESUMO

Due to its central importance to many aspects of evolutionary biology and population genetics, the long-term effective population size (Ne ) has been estimated for numerous species and populations. However, estimating contemporary Ne is difficult and in practice this parameter is often unknown. In principle, contemporary Ne can be estimated using either analyses of temporal changes in allele frequencies, or the extent of linkage disequilibrium (LD) between unlinked markers. We applied these approaches to estimate contemporary Ne of a relatively recently founded island population of collared flycatchers (Ficedula albicollis). We sequenced the genomes of 85 birds sampled in 1993 and 2015, and applied several temporal methods to estimate Ne at a few thousand (4000-7000). The approach based on LD provided higher estimates of Ne (20,000-32,000) and was associated with high variance, often resulting in infinite Ne . We conclude that whole-genome sequencing data offers new possibilities to estimate high (>1000) contemporary Ne , but also note that such estimates remain challenging, in particular for LD-based methods for contemporary Ne estimation.


Assuntos
Aves Canoras , Animais , Genética Populacional , Genoma , Genômica , Desequilíbrio de Ligação , Densidade Demográfica , Aves Canoras/genética
2.
J Wildl Dis ; 48(3): 537-41, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22740518

RESUMO

Yersinia enterocolitica serotype O:9 has identical O-antigens to those of Brucella abortus and has apparently caused false-positive reactions in numerous brucellosis serologic tests in elk (Cervus canadensis) from southwest Montana. We investigated whether a similar phenomenon was occurring in brucellosis antibody-positive bison (Bison bison) using Y. enterocolitica culturing techniques and multiplex PCR of four diagnostic loci. Feces from 53 Yellowstone bison culled from the population and 113 free-roaming bison from throughout the Greater Yellowstone Ecosystem (GYE) were tested. Yersinia enterocolitica O:9 was not detected in any of 53 the bison samples collected at slaughter facilities or in any of the 113 fecal samples from free-ranging bison. One other Y. enterocolitica serotype was isolated; however, it is not known to cause cross-reaction on B. abortus serologic assays because it lacks the perosamine synthetase gene and thus the O-antigens. These findings suggest that Y. enterocolitica O:9 cross-reactivity with B. abortus antigens is unlikely to have been a cause of false-positive serology tests in GYE bison and that Y. enterocolitica prevalence was low in bison in the GYE during this study.


Assuntos
Anticorpos Antibacterianos/sangue , Bison/microbiologia , Yersiniose/veterinária , Yersinia enterocolitica/imunologia , Animais , Brucella abortus/classificação , Brucella abortus/imunologia , Brucelose Bovina/diagnóstico , Brucelose Bovina/epidemiologia , Brucelose Bovina/microbiologia , Bovinos , Reações Cruzadas , Diagnóstico Diferencial , Reações Falso-Positivas , Fezes/microbiologia , Feminino , Masculino , Montana/epidemiologia , Filogenia , Yersiniose/diagnóstico , Yersiniose/epidemiologia , Yersiniose/microbiologia , Yersinia enterocolitica/classificação
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