Vertebrate response to microplastics, nanoplastics and co-exposed contaminants: Assessing accumulation, toxicity, behaviour, physiology, and molecular changes.

Pollution from microplastics (MPs) and nanoplastics (NPs) has gained significant public attention and has become a serious environmental problem worldwide. This review critically investigates MPs/NPs' ability to pass through biological barriers in vertebrate models and accumulate in various organs, including the brain. After accumulation, these particles can alter individuals' behaviour and exhibit toxic effects by inducing oxidative stress or eliciting an inflammatory response. One major concern is the possibility of transgenerational harm, in which toxic consequences are displayed in offspring who are not directly exposed to MPs/NPs. Due to their large and marked surface hydrophobicity, these particles can easily absorb and concentrate various environmental pollutants, which may increase their toxicity to individuals and subsequent generations. This review systematically provides an analysis of recent studies related to the toxic effects of MPs/NPs, highlighting the intricate interplay between co-contaminants in vitro and in vivo. We further delve into mechanisms of MPs/NPs-induced toxicity and provide an overview of potential therapeutic approaches to lessen the negative effects of these MPs/NPs. The review also emphasizes the urgency of future studies to examine the long-term effects of chronic exposure to MPs/NPs and their size- and type-specific hazardous dynamics, and devising approaches to safeguard the affected organisms.

Elemental sulfur enhances the anti-fungal effect of Lacticaseibacillus rhamnosus Lcr35.

Lacticaseibacillus rhamnosus Lcr35 is a well-known bacterial strain whose efficiency in preventing recurrent vulvovaginal candidiasis has been largely demonstrated in clinical trials. The presence of sodium thiosulfate (STS) has been shown to enhance its ability to inhibit the growth of Candida albicans strains. In this study, we confirmed that Lcr35 has a fungicidal effect not only on the planktonic form of C. albicans but also on other life forms such as hypha and biofilm. Transcriptomic analysis showed that the presence of C. albicans induced a metabolic adaptation of Lcr35 potentially associated with a competitive advantage over yeast cells. However, STS alone had no impact on the global gene expression of Lcr35, which is not in favor of the involvement of an enzymatic transformation of STS. Comparative HPLC and gas chromatography-mass spectrometry analysis of the organic phase from cell-free supernatant (CFS) fractions obtained from Lcr35 cultures performed in the presence and absence of STS identified elemental sulfur (S0) in the samples initially containing STS. In addition, the anti-Candida activity of CFS from STS-containing cultures was shown to be pH-dependent and occurred at acidic pH lower than 5. We next investigated the antifungal activity of lactic acid and acetic acid, the two main organic acids produced by lactobacilli. The two molecules affected the viability of C. albicans but only at pH 3.5 and in a dose-dependent manner, an antifungal effect that was enhanced in samples containing STS in which the thiosulfate was decomposed into S0. In conclusion, the use of STS as an excipient in the manufacturing process of Lcr35 exerted a dual action since the production of organic acids by Lcr35 facilitates the decomposition of thiosulfate into S0, thereby enhancing the bacteria's own anti-fungal effect.

Nano polystyrene induced changes in anxiety and learning behaviour are mediated through oxidative stress and gene disturbance in mouse brain regions.

It is widely reported now that nanoplastic particles have potential neurotoxic effects and may disturb central nervous system (CNS) function. However, the mechanism behind these toxic effects still needs to be elucidated. In the current study, we investigated the effects of polystyrene nanoplastics (PS-NPs) on changes in learning, memory, and anxiety-related behavior in mice based on some selected biochemical, molecular, and histopathological changes in three important brain regions (Cortex, Hypothalamus, and Hippocampus). Male mice were orally administered daily with two doses of 50 nm PS-NPs (0.2 mg/ml and 1 mg/ml) for 8 weeks. We observed decreased expression of neurotransmitter-related genes (VAChT, GAD, and SYP) in the cortex, hypothalamus, and hippocampus areas of the mouse brain. Other biochemical variables including, antioxidant enzymes, biomarkers for oxidative stress, and acetylcholinesterase activity showed significant alterations in all three brain regions. Molecular and neurochemical data thus suggest significant neurobehavioral changes following sub-chronic exposure to PS-NPs which may lead to enhanced anxiety-related and spatial learning and memory-related impairments by affecting limbic areas of the brain.