[Ocular complications in intense pulsed light therapy (case study)]. / Glaznye oslozhneniya intensivnoi fototerapii (klinicheskoe nablyudenie).

A 24-year-old female patient with photophobia, discoria, redness and dryness in her right eye after intense pulsed light (IPL) therapy applied for posttraumatic subcutaneous hemorrhage received a comprehensive ophthalmic examination including optical coherence tomography (OCT) and OCT angiography of the anterior eye segment. Her best corrected visual acuity was 20/20 in both eyes. Medically induced mydriasis revealed discoria with paresis of pupil dilatator in the left eye (pupil size 4.2 mm and 6.6 mm in the right and left eye, respectively). Anterior segment OCT showed anterior chamber cytosis and increased iris vascularity. The patient was prescribed topical 1.0% tropicamide 2 times per day and 1.0% dexamethasone 4 times per day for two weeks. Examination performed after 3 months showed no restoration of pupil dilatator function in the left eye. The case demonstrates potential ocular complications of IPL therapy, which may include iris burn with iritis and persistent pupil dilatator dysfunction.

Genotypic and ecological variability of zinc content in the grain of spring bread wheat varieties in the international nursery KASIB.

Spring bread wheat is the staple crop in Western Siberia and Kazakhstan, a signif icant portion of which goes for export. Wheat breeding with a high level of zinc in wheat grain is the most cost-effective and environmentally friendly way to address zinc def iciency in the diet. The purpose of this work was to evaluate the contribution of the factors 'location' and 'genotype' in the variability of zinc content in wheat grain, and to identify the best varieties as sources of this trait for breeding. The research on screening zinc content in the wheat grain of 49 spring bread wheat varieties from the Kazakhstan- Siberia Spring Wheat Trial (KASIB) nursery was carried out at 4 sites in Russia (Chelyabinsk, Omsk, Tyumen, Novosibirsk) and 2 sites in Kazakhstan (Karabalyk and Shortandy) in 2017-2018. The content of zinc in wheat grain was evaluated at the Ionomic Facility of University of Nottingham in the framework of the EU project European Plant Phenotyping Network-2020. The analysis of variance showed that the main contribution into the general phenotypic variation of the studied trait, 38.7 %, was made by the factor 'location' due to different contents of zinc and moisture in the soil of trial sites; the effect of the factor 'year' was 13.5 %, and the effect of the factor 'genotype' was 8.0 %. The most favorable environmental conditions for accumulation of zinc in wheat grain were observed in the Omsk region. In Omsk, the average zinc content in all studied varieties was 50.4 mg/kg, with 63.7 mg/ kg in the best variety 'OmGAU 100'. These values are higher than the target values of the international program Harvest Plus. 'Novosibirskaya 16' (49.4 mg/kg), 'Silach' (48.4 mg/kg), 'Line 4-10-16' (47.2 mg/ kg), 'Element 22' (46.3 mg/kg) and 'Lutescens 248/01' (46.0 mg/kg) were identif ied as being the best varieties. Signif icant possibilities for the production of wheat grain with high zinc content, which is in demand for the production of bread and pastry products with functional properties, were identif ied in the Western Siberian region.

Efficacy of navigated focal laser photocoagulation in diabetic macular edema planned with en face optical coherence tomography versus fluorescein angiography.

AIM: To analyze the efficacy of navigated focal laser photocoagulation (FLP) of microaneurysms in diabetic macular edema (DME) planned using en face optical coherence tomography (OCT) as against fluorescein angiography (FA). METHODS: Twenty-six eyes of 21 DME patients (12 males, 9 females, 69.5 ± 12.3 years) with mean BCVA of 0.52 ± 0.44 LogMAR were included. En face OCT images of deep capillary plexus slab and FA images were used to plan FLP targeting of leaky microaneurysms. The primary outcome measures were central retinal thickness (CRT) and macular volume. The secondary outcome measure was best-corrected visual acuity (BCVA). RESULTS: The difference in the change of CRT and macular volume between en face OCT and FA-planned FLP after 1 month and at the end of follow-up was not statistically significant (p > 0.05), except for a higher CRT reduction in the en face OCT-planning group (p = 0.007) at the end of mean follow-up of 2.6 ± 0.9 months. There was no difference in BCVA change between the two planning options (p = 0.42). CONCLUSION: En face OCT is a non-inferior alternative for FA in the planning of navigated FLP of microaneurysms in DME.

[Comparative analysis of modern approaches to risk estimation from artificially created nanoparticles and nanomaterials].

The article represents a review of modern approaches to problem of nanotechnologies and nanomaterials risk estimation for human health and environment that were elaborated in EU, USA and some international authorities. Submitted data suggests that there is a significant coincidence with approaches being developed and introduced in Russian Federation under the guidance of Rospotrebnadzor. Particularly criteria being used in Russian Federation and EU for classification of nanotechnologies and nanoindustry production are mainly similar. They include a) identification of nanomaterials in production, b) establishment of production disintegration possibility with concomitant migration of free nanoparticles, c) possibility of nanoparticles emission/migration both in normal conditions of utilization and in possible emergency conditions, d) degree of proximity of particular kind of production to it's consumer that means the possibility of exposition being from closely to zero (in conditions of unhabitated exploitation) up to approximately 100% (in case of medicine, food and cosmetics), e) biological examination of potential danger of nanomaterials according to current volume of scientific information. As applied to nanotechnology plants there are such criteria in use as a) nanomaterial identification, b) personnel exhibiting possibility, c) potential toxicity of stuff in aerosol nano-form, d) characteristics of biological action. Thus applied in Russia principles for nanomaterials safety estimation do not contradict to concepts of foreign authorities that builds up a possibility of said approaches harmonization to internationally recognized norms.

[Electron microscopic structure of the Oidium, Oospora fragrans, a producer of lipase]. / Elektronno-mikroskopicheskoe stroenie oidii Oospora fragrans--produtsenta lipasy.

The fungus Oospora fragrans Berkh. was studied by electron microscopy. Both aerial and submerged oidia were found to be formed by division (septation) of mycelial hyphae in the course of growth. The ultrastructure of the aerial and submerged oidia was identical as was established by the techniques of ultratomy and freezing-etching. The latter technique revealed a certain difference in the morphology of the cytoplasmic membrane of these cells. The oidia of this fungus (arthrospores) differed in their structure from the arthrospores of actinomycetes.

[Pigment and antibiotic substance contained in the spores of Actinomyces streptomycini B-6]. / Pigment i antibioticheskoe veshchestvo, soderzhashcheesia v sporakh Actinomyces streptomycini B-6

The antibiotic activity and fractional composition of the supernatant of the homogenate of Actinomyces streptomycini B-6 spores were investigated. The homogenate supernatant was of yellow colour and showed antibiotic activity when used as the test-organism of the culture St. aureus 209. The antibiotic activity was associated not with the homogenate pigment but with the colourless substance referring to streptomycins which was contained in spores.