Assuntos
Betacoronavirus , Infecções por Coronavirus/prevenção & controle , Controle de Infecções/organização & administração , Transmissão de Doença Infecciosa do Paciente para o Profissional/prevenção & controle , Exposição Ocupacional/prevenção & controle , Pandemias/prevenção & controle , Pneumonia Viral/prevenção & controle , Tuberculose/prevenção & controle , COVID-19 , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/transmissão , Humanos , Pneumonia Viral/diagnóstico , Pneumonia Viral/transmissão , SARS-CoV-2 , Tuberculose/diagnóstico , Tuberculose/transmissãoRESUMO
BACKGROUND: Public health facilities are used by the majority of South Africans, and healthcare utilisation surveys have been a useful tool to estimate the burden of disease in a given area. OBJECTIVES: To describe care-seeking behaviour in a periurban site with a high prevalence of HIV infection, as well as barriers to seeking appropriate healthcare. METHODS: We conducted a cross-sectional household survey in 22 wards of the Msunduzi municipality in KwaZulu-Natal Province, South Africa, from October to December 2013 using a simple random sample of households selected from a 2011 census enumeration. A primary caregiver/adult decision-maker was interviewed regarding demographic data as well as health status and recent self-reported episodes of selected illnesses and healthcare utilisation. RESULTS: Of the 2 238 eligible premises visited, 1 936 households (87%) with a total of 9 733 members were enrolled in the study. Of these, 635 (7%) reported one or more episodes of infectious illness during the study period. Public health clinics were most frequently consulted for all illnesses (361/635, 57%). Private healthcare (general practitioner, private clinic, private hospital) was sought by 90/635 of individuals (14%), only 13/635 (2%) reported seeking care from traditional healers, religious leaders or volunteers, and 71/635 (11%) did not seek any medical care for acute illnesses. Individuals in the lowest income group were more likely to seek care at public health facilities than those in the highest income group (70% v. 32%). CONCLUSIONS: Public health facility-based surveillance may be representative of disease patterns in this community, although surveillance at household level shows that high-income individuals may be excluded because they were more likely to use private healthcare, and the proportion of individuals who died at home would have been missed by facility-based surveillance. Data obtained in such surveys may be useful for public health planning.
Assuntos
Atenção à Saúde/estatística & dados numéricos , Gastroenteropatias/epidemiologia , Meningite/epidemiologia , Aceitação pelo Paciente de Cuidados de Saúde/estatística & dados numéricos , Doenças Respiratórias/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Estudos Transversais , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , África do Sul/epidemiologia , Síndrome , Adulto JovemRESUMO
Public health interest in norovirus (NoV) has increased in recent years following improved diagnostics, global burden estimates and the development of NoV vaccine candidates. This study aimed to describe the detection rate, clinical characteristics and environmental features associated with NoV detection in hospitalized children <5 years with diarrhoea in South Africa (SA). Between 2009 and 2013, prospective diarrhoeal surveillance was conducted at four sites in SA. Stool specimens were collected and screened for NoVs and other enteric pathogens using molecular and serological assays. Epidemiological and clinical data were compared in patients with or without detection of NoV. The study detected NoV in 15% (452/3103) of hospitalized children <5 years with diarrhoea with the majority of disease in children <2 years (92%; 417/452). NoV-positive children were more likely to present with diarrhoea and vomiting (odds ratio (OR) 1·3; 95% confidence interval (CI) 1·1-1·7; P = 0·011) with none-to-mild dehydration (adjusted OR 0·5; 95% CI 0·3-0·7) compared with NoV-negative children. Amongst children testing NoV positive, HIV-infected children were more likely to have prolonged hospitalization and increased mortality compared with HIV-uninfected children. Continued surveillance will be important to consider the epidemic trends and estimate the burden and risk of NoV infection in SA.
Assuntos
Infecções por Caliciviridae/epidemiologia , Diarreia/epidemiologia , Norovirus/fisiologia , Infecções por Caliciviridae/virologia , Pré-Escolar , Diarreia/virologia , Hospitalização/estatística & dados numéricos , Humanos , Lactente , Recém-Nascido , Estudos Prospectivos , África do Sul/epidemiologiaRESUMO
OBJECTIVES: Shigella sonnei is a globally important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in PulseNet International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed to demonstrate the possibility and advantages of transitioning to whole genome sequencing (WGS) for surveillance within existing networks across a continent where S. sonnei is endemic. METHODS: We applied WGS to representative archive isolates of S. sonnei (n = 323) from laboratories in nine PNLA&C countries to generate a regional phylogenomic reference for S. sonnei and put this in the global context. We used this reference to contextualise 16 S. sonnei from three Argentinian outbreaks, using locally generated sequence data. Assembled genome sequences were used to predict antimicrobial resistance (AMR) phenotypes and identify AMR determinants. RESULTS: S. sonnei isolates clustered in five Latin American sublineages in the global phylogeny, with many (46%, 149 of 323) belonging to previously undescribed sublineages. Predicted multidrug resistance was common (77%, 249 of 323), and clinically relevant differences in AMR were found among sublineages. The regional overview showed that Argentinian outbreak isolates belonged to distinct sublineages and had different epidemiologic origins. CONCLUSIONS: Latin America contains novel genetic diversity of S. sonnei that is relevant on a global scale and commonly exhibits multidrug resistance. Retrospective passive surveillance with WGS has utility for informing treatment, identifying regionally epidemic sublineages and providing a framework for interpretation of prospective, locally sequenced outbreaks.
Assuntos
Disenteria Bacilar , Doenças Transmitidas por Alimentos , Shigella sonnei/genética , Região do Caribe/epidemiologia , DNA Bacteriano/análise , DNA Bacteriano/genética , Surtos de Doenças/prevenção & controle , Surtos de Doenças/estatística & dados numéricos , Farmacorresistência Bacteriana , Disenteria Bacilar/epidemiologia , Disenteria Bacilar/microbiologia , Doenças Transmitidas por Alimentos/epidemiologia , Doenças Transmitidas por Alimentos/microbiologia , Humanos , América Latina/epidemiologia , Vigilância em Saúde Pública , Estudos Retrospectivos , Shigella sonnei/efeitos dos fármacos , Sequenciamento Completo do GenomaAssuntos
Antibacterianos/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Farmacorresistência Bacteriana , Promoção da Saúde/organização & administração , Medicamentos sob Prescrição/uso terapêutico , Atenção Primária à Saúde/organização & administração , Infecções Bacterianas/epidemiologia , Humanos , Programas Nacionais de Saúde/organização & administração , África do SulAssuntos
Controle de Doenças Transmissíveis/métodos , Doenças Transmissíveis/epidemiologia , Farmacorresistência Bacteriana , Promoção da Saúde/organização & administração , Vigilância de Evento Sentinela , Antibacterianos/uso terapêutico , Doenças Transmissíveis/tratamento farmacológico , Humanos , Programas Nacionais de Saúde/organização & administração , África do SulRESUMO
In 2005, over 600 clinically diagnosed typhoid fever cases occurred in South Africa, where an outbreak had been previously described in 1993. Case-control and molecular investigations, including Salmonella enterica serovar Typhi (S. Typhi) isolates from that area from 1993, 2005 and later, were undertaken. Controls were significantly older than cases (P=0·003), possibly due to immunity from previous infection, and a significantly larger proportion had attended a gathering (P=0·035). Exposure to commercial food outlets and person-to-person transmission was not significant. Pulsed-field gel electrophoresis and multi-locus tandem repeat analysis revealed common clusters of S. Typhi strains identified in 1993 and 2005 as well as in 2007 and 2009. This outbreak probably occurred in a non-immune population due to faecally contaminated water. S. Typhi strains appeared to be related to strains from 1993; failure to address unsafe water may lead to further outbreaks in the area if the current population immunity wanes or is lost.
Assuntos
Surtos de Doenças , Salmonella typhi/classificação , Salmonella typhi/genética , Febre Tifoide/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Criança , Pré-Escolar , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Tipagem Molecular , Salmonella typhi/isolamento & purificação , África do Sul/epidemiologia , Adulto JovemRESUMO
In South Africa, for the years 2003-2007, the Enteric Diseases Reference Unit received 510 human isolates of Salmonella Typhi, of which 27 were nalidixic acid-resistant [minimum inhibitory concentrations (MICs) 128-512 microg/ml] with reduced susceptibility to ciprofloxacin (MICs 0.125-0.5 microg/ml). Pulsed-field gel electrophoresis analysis of 19 available isolates differentiated them into five DNA pattern types; multiple-locus variable-number tandem repeat analysis differentiated the isolates into 10 types. This level of genetic diversity suggested that resistant strains usually emerged independently of one another. A 16- to 32-fold decrease in nalidixic acid MIC and a 2- to 8-fold decrease in ciprofloxacin MIC, was observed in the presence of an efflux pump inhibitor. All isolates were negative by PCR screening for qnr genes. Seven resistant isolates were further analysed for mutations in the quinolone resistance-determining region of gyrA, gyrB, parC and parE. No amino-acid mutations were identified in GyrB and ParE; all isolates showed amino-acid mutations in both GyrA and ParC. We conclude that amino-acid mutations in GyrA and ParC in combination with active efflux of antibiotic out of the bacterial cell are the probable mechanisms conferring quinolone resistance.
Assuntos
Farmacorresistência Bacteriana Múltipla/genética , Salmonella typhi/efeitos dos fármacos , Salmonella typhi/genética , Febre Tifoide/epidemiologia , Ciprofloxacina/metabolismo , DNA Girase/genética , DNA Topoisomerase IV/genética , Farmacorresistência Bacteriana Múltipla/fisiologia , Genótipo , Humanos , Testes de Sensibilidade Microbiana , Ácido Nalidíxico/metabolismo , Prevalência , Salmonella typhi/isolamento & purificação , África do Sul/epidemiologia , Febre Tifoide/microbiologiaRESUMO
We report on the first recorded outbreak of cholera in Namibia. From December 2006 to February 2007, more than 250 cases of cholera were reported from the Omusati and Kunene provinces of Namibia. However, only nine bacterial isolates were obtainable for analysis. Isolates were all identified as Vibrio cholerae O1 serotype Inaba biotype El Tor. All isolates were susceptible to ampicillin, augmentin, chloramphenicol, nalidixic acid, ciprofloxacin, tetracycline, kanamycin, imipenem, ceftriaxone and ceftazidime; and they all showed resistance to trimethoprim, sulfamethoxazole and streptomycin. Pulsed-field gel electrophoresis analysis of bacteria incorporating either SfiI or NotI digestion revealed an identical fingerprint pattern for all isolates. These data together with results indicating identical antimicrobial susceptibility profiles for all isolates determined that the outbreak was caused by a single strain of V. cholerae.
Assuntos
Cólera/epidemiologia , Cólera/microbiologia , Surtos de Doenças , Vibrio cholerae O1/isolamento & purificação , Adolescente , Adulto , Técnicas de Tipagem Bacteriana , Criança , Pré-Escolar , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Lactente , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Namíbia/epidemiologia , Vibrio cholerae O1/classificação , Vibrio cholerae O1/efeitos dos fármacosRESUMO
Since May 2000, extended-spectrum beta-lactamase-producing (ESBL) Salmonella Isangi were isolated from pediatric patients at a tertiary hospital. A total of 41 patients with positive cultures were reviewed, and the majority presented with gastroenteritis, fever, or both. One ESBL phenotype was noted in all isolates, and clonality was confirmed by pulsed-field gel electrophoresis. This is the first report of Salmonella sp. ESBL resistance in our hospital.
Assuntos
Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Surtos de Doenças , Infecções por Salmonella/epidemiologia , Salmonella enterica/isolamento & purificação , beta-Lactamases/biossíntese , Pré-Escolar , Eletroforese em Gel de Campo Pulsado/métodos , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Infecções por Salmonella/microbiologia , Salmonella enterica/enzimologia , Resistência beta-LactâmicaRESUMO
The characteristics of Vibrio cholerae O1 biotype El Tor, serotype Ogawa isolates from outbreaks of cholera in 1998 amongst migrant workers in the South African provinces of Gauteng and Mpumalanga, on the border of Mozambique, are reported. The isolates seem to have originated from the same clone since they are of two closely related BglI ribotypes. These ribotypes had a high similarity to ribotypes of V. cholerae O1 recently found in three South-east Asian countries. Isolates were resistant to furazolidone, streptomycin, sulfamethoxazole, trimethoprim and tetracycline. Only two isolates contained plasmids of 54 and 63 kb in size. PCR and DNA sequencing revealed that the chromosomally located resistance determinants present included an aadA2 gene cassette contained in a class 1 integron; the SXT element, which is a transposon-like element containing resistance genes; and the tetA gene. A co-transfer of chromosomal closely located genes encoding the SXT element and tetA was shown by mating experiments, PCR and pulsed-field gel electrophoresis analyses. Our study shows for the first time that multiple-resistant V. cholerae O1 isolates containing class 1 integrons and the SXT element were responsible for cholera outbreaks in Southern Africa. Studies are needed to determine the spread of this multiple-resistant O1 strain and further genetic details of the association of the SXT element, tetA and class 1 integrons, including their means of transfer.
Assuntos
Cólera/epidemiologia , Surtos de Doenças , Farmacorresistência Bacteriana Múltipla/genética , Nucleotidiltransferases/genética , Vibrio cholerae/genética , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Cólera/tratamento farmacológico , Cólera/genética , Elementos de DNA Transponíveis/genética , Surtos de Doenças/estatística & dados numéricos , Humanos , Testes de Sensibilidade Microbiana/métodos , Moçambique/epidemiologia , África do Sul/epidemiologia , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/isolamento & purificaçãoAssuntos
Proteção da Criança/estatística & dados numéricos , Surtos de Doenças/prevenção & controle , Surtos de Doenças/estatística & dados numéricos , Doenças Transmitidas por Alimentos/epidemiologia , Doenças Transmitidas por Alimentos/prevenção & controle , Cooperação Internacional , Esportes , Saúde da População Urbana/estatística & dados numéricos , Criança , Notificação de Doenças , Prioridades em Saúde , Humanos , Avaliação das Necessidades , Prática de Saúde Pública , África do Sul/epidemiologiaAssuntos
Bactérias/genética , Resistência Microbiana a Medicamentos , Saúde Global , Humanos , Mutação , ViagemRESUMO
Between 10 and 11 years after children were vaccinated with Vi capsular polysaccharide of Salmonella typhi or meningococcal A + C control vaccine in a double blind randomized trial, we traced 83 subjects, aged 16-20 years. A blood sample was taken for determination of Vi antibody titres in both groups by radioimmunoassay. TO and TH titres were also done to assess if the participants had had recent exposure to typhoid fever. Fifty-eight percent of subjects in both groups had protective levels of Vi antibody against Salmonella typhi (a titre greater than 1 microgram ml-1). There was no significant difference in the levels of Vi antibodies in the cases versus the controls (p = 0.5). Two of the children who had received meningococcal A + C vaccine had recently had typhoid fever. Our data show that adolescents in typhoid endemic areas have high levels of Vi antibodies regardless of previous vaccination status, suggesting that Vi antibodies are acquired in adolescence by a large percentage of the population in this area. Moreover, Vi vaccination has led to ongoing antibody production in greater than 50% of Vi vaccinated children in an endemic area for a period of 10 years. Ongoing antigenic exposure may have contributed to these antibody levels.
Assuntos
Anticorpos Antibacterianos/sangue , Salmonella typhi/imunologia , Vacinas Tíficas-Paratíficas/imunologia , Adolescente , Adulto , Vacinas Bacterianas/administração & dosagem , Vacinas Bacterianas/imunologia , Método Duplo-Cego , Seguimentos , Humanos , Vacinas Meningocócicas , Polissacarídeos Bacterianos/imunologia , África do Sul , Fatores de Tempo , Vacinas Tíficas-Paratíficas/administração & dosagemAssuntos
Polissacarídeos Bacterianos/farmacologia , Salmonella typhi/imunologia , Febre Tifoide/prevenção & controle , Vacinas Tíficas-Paratíficas/farmacologia , Chile , Ensaios Clínicos como Assunto , Humanos , Polissacarídeos Bacterianos/imunologia , Salmonella typhi/isolamento & purificação , África do Sul , Febre Tifoide/imunologia , Febre Tifoide/microbiologia , Vacinas Tíficas-Paratíficas/imunologiaRESUMO
OBJECTIVE: To document the incidence of high-level gentamicin resistance (HLGR) in enterococcal isolates at Johannesburg Hospital. DESIGN: Survey of laboratory isolates. SETTING: Academic hospitals. BACTERIAL STRAINS: Consecutive samples of enterococcal isolates. MAIN OUTCOME MEASURE: The incidence of HLGR in enterococcal isolates. RESULTS: The incidence of HLGR was 26.5% of Enterococcus faecalis isolates and 20% of E. faecium isolates grown during the study period. CONCLUSIONS: High-level gentamicin resistance is common among enterococci isolated at Johannesburg Hospital, and this observation must be considered in defining strategies for the management of invasive enterococcal infections in the future.
Assuntos
Antibacterianos , Resistência Microbiana a Medicamentos , Gentamicinas , Incidência , Antibacterianos/administração & dosagem , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/isolamento & purificação , Enterococcus faecium/efeitos dos fármacos , Enterococcus faecium/isolamento & purificação , Gentamicinas/administração & dosagem , Gentamicinas/farmacologia , Gentamicinas/uso terapêutico , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/epidemiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Hospitais de Ensino , Humanos , África do Sul/epidemiologiaRESUMO
The objectives of this study were (a) to compare the CD4+ lymphocyte profiles over time of two groups of patients hospitalized for tuberculosis (TB) treatment [a group of patients with TB only (TB group) and a group dually infected by HIV and TB (HIV/TB group)] and (b) to assess the usefulness of the total lymphocyte count (TLC) as a surrogate of the CD4+ lymphocyte count in the HIV/TB group. A total of 345 patients were enrolled in the study of whom 104 (29.8%) were HIV seropositive (HIV/TB). On admission, the CD4+ lymphocyte counts of the HIV/TB cohort were significantly lower than the TB group with medians of 230 (interquartile range, 90-475) and 630 (500-865), respectively (p < 0.0001). The CD4+ lymphocyte count increased significantly in both cohorts on routine TB treatment. A TLC of 1,300-1,500 cells/mm3 was found to be predictive of a CD4+ lymphocyte count of < or = 200 cells/mm3 both on admission and after 1 month of TB therapy. We conclude from this study that the positive influence of TB therapy on the CD4+ lymphocyte count strongly suggests an additional avenue of influence on the course of HIV infection, whereas the usefulness of the TLC as a surrogate estimation of CD4+ lymphocyte count in HIV/TB patients has important implications for the developing world.