Chaetomium and Chaetomium-like Species from European Indoor Environments Include Dichotomopilus finlandicus sp. nov.

The genus Chaetomium is a frequently occurring fungal taxon world-wide. Chaetomium and Chaetomium-like species occur in indoor environments, where they can degrade cellulose-based building materials, thereby causing structural damage. Furthermore, several species of this genus may also cause adverse effects on human health. The aims of this research were to identify Chaetomium and Chaetomium-like strains isolated from indoor environments in Hungary and Finland, two geographically distant regions of Europe with drier and wetter continental climates, respectively, and to study their morphological and physiological properties, as well as their extracellular enzyme activities, thereby comparing the Chaetomium and Chaetomium-like species isolated from these two different regions of Europe and their properties. Chaetomium and Chaetomium-like strains were isolated from flats and offices in Hungary, as well as from schools, flats, and offices in Finland. Fragments of the translation elongation factor 1α (tef1α), the second largest subunit of RNA polymerase II (rpb2) and ß-tubulin (tub2) genes, as well as the internal transcribed spacer (ITS) region of the ribosomal RNA gene cluster were sequenced, and phylogenetic analysis of the sequences performed. Morphological examinations were performed by stereomicroscopy and scanning electron microscopy. Thirty-one Chaetomium sp. strains (15 from Hungary and 16 from Finland) were examined during the study. The most abundant species was Ch. globosum in both countries. In Hungary, 13 strains were identified as Ch. globosum, 1 as Ch. cochliodes, and 1 as Ch. interruptum. In Finland, 10 strains were Ch. globosum, 2 strains were Ch. cochliodes, 2 were Ch. rectangulare, and 2 isolates (SZMC 26527, SZMC 26529) proved to be representatives of a yet undescribed phylogenetic species from the closely related genus Dichotomopilus, which we formally describe here as the new species Dichotomopilus finlandicus. Growth of the isolates was examined at different temperatures (4, 15, 20, 25, 30, 37, 35, 40, and 45 °C), while their extracellular enzyme production was determined spectrophotometrically.

Chronic responses of aerobic granules to the presence of graphene oxide in sequencing batch reactors.

The chronic responses of aerobic granular sludge (AGS) to the presence of graphene oxide nanoparticles (GO NPs) (5, 15, 25, 35, 45, 55, 65, 75, 85, and 95 mg/L of GO NPs for 7 days) during biological wastewater treatment processes were investigated. Bioreactor performance, extracellular polymeric substance (EPS) secretion, and microbial community characteristics were assessed. The results showed that the effects of GO NPs on bioreactor performances were dependent on the dose applied and the duration for which it was applied. At concentrations of 55, 75, and 95 mg/L, GO NPs considerably inhibited the efficiency of organic matter and ammonia removal; however, nitrite and nitrate removal rates were unchanged. Biological phosphorus removal decreased even when only low concentrations of GO NPs were used. The secretion of EPS, which could alleviate the toxicity of GO NPs, also changed. The increased amount of nanoparticles also resulted in significant changes to the bacterial community structure. Based on the amplicon sequencing of 16S rRNA genes, Paracoccus sp., Klebsiella sp., and Acidovorax species were identified as the most tolerant strains.