Citrus photosynthesis and morphology acclimate to phloem-affecting huanglongbing disease at the leaf and shoot levels.

Huanglongbing (HLB) is a phloem-affecting disease in citrus that reduces growth and impacts global citrus production. HLB is caused by a phloem-limited bacterium (Candidatus Liberibacter asiaticus). By inhibiting phloem function, HLB stunts sink growth, including the production of new shoots and leaves, and induces hyperaccumulation of foliar starch. HLB induces feedback inhibition of photosynthesis by reducing foliar carbohydrate export. Here, we assessed the relationship of bacterial distribution within the foliage, foliar starch accumulation, and net CO2 assimilation (Anet ). Because HLB impacts canopy morphology, we developed a chamber to measure whole-shoot Anet to test the effects of HLB at both the leaf and shoot level. Whole-shoot level Anet saturated at high irradiance, and green stems had high photosynthetic rates compared to leaves. Starch accumulation was correlated with bacterial population, and starch was negatively correlated with Anet at the leaf but not at the shoot level. Starch increased initially after infection, then decreased progressively with increasing length of infection. HLB infection reduced Anet at the leaf level but increased it at the whole-shoot level, in association with reduced leaf size and greater relative contribution of stems to the photosynthetic surface area. Although HLB-increased photosynthetic efficiency, total carbon fixed per shoot decreased because photosynthetic surface area was reduced. We conclude that the localized effects of infection on photosynthesis are mitigated by whole-shoot morphological acclimation over time. Stems contribute important proportions of whole-shoot Anet , and these contributions are likely increased by the morphological acclimation induced by HLB.

Short-term warming does not affect intrinsic thermotolerance but induces strong sustaining photoprotection in tropical evergreen citrus genotypes.

Consequences of warming and postwarming events on photosynthetic thermotolerance (PT ) and photoprotective responses in tropical evergreen species remain elusive. We chose Citrus to answer some of the emerging questions related to tropical evergreen species' PT behaviour including (i) how wide is the genotypic variation in PT ? (ii) how does PT respond to short-term warming and (iii) how do photosynthesis and photoprotective functions respond over short-term warming and postwarming events? A study on 21 genotypes revealed significant genotypic differences in PT , though these were not large. We selected five genotypes with divergent PT and simulated warming events: Tmax 26/20°C (day-time highest maximum/night-time lowest maximum) (Week 1) < Tmax 33/30°C (Week 2) < Tmax 36/32°C (Week 3) followed by Tmax 26/16°C (Week 4, recovery). The PT of all genotypes remained unaltered despite strong leaf megathermy (leaf temperature > air temperature) during warming events. Though moderate warming showed genotype-specific stimulation in photosynthesis, higher warming unequivocally led to severe loss in net photosynthesis and induced higher nonphotochemical quenching. Even after a week of postwarming, photoprotective mechanisms strongly persisted. Our study points towards a conservative PT in evergreen citrus genotypes and their need for sustaining higher photoprotection during warming as well as postwarming recovery conditions.