RESUMO
The Ferula assa-foetida (FA) is the healthy common-consumed anticancer beverage in Iranian folk medicine. In the current study, we aimed to produce a nanoemulsion-based drug delivery system containing FA essential oil (FAEO) and evaluate its antioxidant and anticancer activity on both MCF-7 cells and murine mammary cancer tissue. The FAEO-loaded nanoemulsion (FAEO-NE) was produced and characterized by DLS, TEM, FTIR, and Zeta potential analysis. Radical (ABTS and DPPH) scavenging activity, cytotoxic, apoptotic, and anti-angiogenic potentials of the FAEO-NE were studied by applying antioxidant (ABTS-DPPH), MTT, AO/PI cell staining, and Q-PCR analysis. Finally, its anti-tumor impact was evaluated on murine mammary tumor models. The FAEO-NE exhibited a meaningful antioxidant activity. Also, its significant cell-selective cytotoxic, apoptotic, and anti-angiogenic impacts on MCF-7 cancer cells indicated its anticancer potential. Moreover, the progressive destruction of the murine mammary glands cancer tissue confirmed their anticancer activity. Regarding the FAEO-NE cell-selective cytotoxic, apoptotic, and anti-angiogenic activity on MCF-7 breast cancer cells, it has the potential to be studied as a safe efficient anti-breast cancer agent.
Assuntos
Antineoplásicos , Neoplasias da Mama , Ferula , Óleos Voláteis , Animais , Antineoplásicos/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Feminino , Ferula/química , Humanos , Irã (Geográfico) , Células MCF-7 , Camundongos , Óleos Voláteis/farmacologiaRESUMO
Loading of the Brassica napus extract (BNE) on PLGA nanoparticle (BNE-PNP) and study its necroptotic activity in human MCF7-breast cancer cells. Double emulsion solvent evaporation methods were used for synthesis of BNE-PNP and DLS, SEM, and surface Zeta-potential analysis were applied for defining the physicochemical properties of BNE-PNP. The cytotoxic impact of BNE-PNP nanoparticles was analyzed by MTT assay and expression of apoptotic (P53 and Cas-3) and necrotic (TNF-α) gene markers were measured by qPCR to evaluate the BNE-PNP-induced cell death type. The stable (-36.07 mV) BNE-PNP were synthesized at 71.07 nm dimension. They significantly decrease the count of metabolically active MCF7 cells (IC50: 170.94 µg/ml after 48 h). The BNE-PNP induced an early programmed necrotic (necroptosis) and late apoptotic death on the MCF7 cancer cells by up-regulating all the P53/TNF-α and Cas-3 gene expression, respectively. The BNE-PNP dose-dependently induced an early cell-selective necroptotic death. Since the necroptotic death is known as a biocompatible cellular death induction, the BNE-PNP have the potential to be used as a safe efficient anticancer compound.