Comparative Evaluation of Push-out Bond Strength of Three Different Root Canal Sealers: An In Vitro Study.

AIM: The aim of this present study was to compare the dislodgement resistance of calcium silicate-based sealer, zinc oxide sealer, and a new sealer combining both zinc oxide and calcium silicate-based sealer in vitro. MATERIALS AND METHODS: 60 single-rooted human teeth were instrumented with F3 Protaper Gold. All endodontic canals were filled using gutta percha cones using the cold lateral condensation technique in combination using one of the mentioned sealers (n = 20 per group). The teeth were divided into three groups: group A consisted of Sealite® Ultra, group B consisted of K-Sealer®, and group C consisted of BioRoot® RC. After 2 months of incubation (37°C, 100% humidity) and after cutting out 2 mm from the most apical portion of the root apex, six slices of 1 mm thickness were generated. Mechanical dislodgement resistance was examined using a universal pressure-testing machine and the push-out bond strength (POBS) was calculated. Specimens were examined under 20× magnification to define the bond failure mode. Statistical analysis was executed using ANOVA, post hoc Turkey test for pairwise comparisons and Kruskal-Wallis tests. RESULTS: The POBS of BioRoot® was significantly higher than the POBS of the two other sealers with a mean of 10.54 MPa ± 2.10 and 5.73 MPa ± 2.34, respectively (p < 0.001). Sealite® and K-Sealer® showed similar results in the median and coronal part. K-Sealer® revealed highest POBS compared with Sealite® in the apical part (p < 0.05). CONCLUSION: The POBS of the zinc oxide and calcium silicate-based sealer was significantly lower compared with calcium silicate. Sealite® and K-Sealer® exhibited almost same results. BioRoot showed the highest POBS of all sealers. CLINICAL SIGNIFICANCE: The current study was needed to evaluate the bond strength of three different cements to dentinal walls, by evaluating their respective POBS in vitro. The findings of this study may provide guidance for the clinician in the selection of an adequate endodontic sealer that guarantees an enhanced adhesive seal between the Gutta-percha and the dentinal canal walls. How to cite this article: Makhlouf MP, El Helou JD, Zogheib CE, et al. Comparative Evaluation of Push-out Bond Strength of Three Different Root Canal Sealers: An In Vitro Study. J Contemp Dent Pract 2024;25(1):15-19.

Effect of heat application on the physicochemical properties of new endodontic sealers: an in vitro and SEM study.

Understanding the properties of endodontic sealers is vital for treatment planning. Calcium silicate-based sealers are important in modern endodontics. This study investigates the effect of heat on the physicochemical properties of new calcium silicate sealers, addressing concerns raised by clinicians seeking to combine their benefits with the gutta-percha obturation technique. Five endodontic sealers (AH Plus Bioceramic®, Total Fill® BC®, One-fil® Bioceramic, K-Biocer, Any-seal®) were evaluated. Each sealer (n = 16/group) was either kept at 37 °C or subjected to heat at 60 or 200 °C for 30 s. ISO 6876-2012 standards were used to measure setting time, flow, film thickness, and dimensional changes over time. SEM and EDS were utilized for surface and chemical analysis. Data analysis employed IBM SPSS Statistics version 26 with a 5% significance level for two-sided tests. The sealers' setting times were shortened by heat, except for Total Fill® BC with extended setting time. All sealers had significantly longer setting times than manufacturer specifications. Film thickness increased with temperature, while flow values decreased. K-Biocer sealer showed the highest flow (16.89 ± 0.57 mm) at 200 °C, while Total Fill® sealer had the lowest (15.32 ± 0.62 mm). Shrinkage was significant at 60 °C and 200 °C, with greater shrinkage at 200 °C. Heat caused surface deformations in all sealers. The 200 °C groups exhibited more voids in AH Plus Bioceramic®, Any-seal®, and One-fil® sealers, and higher void area in Total Fill®, One-fil®, and K-Biocer sealers (p value < 0.001). EDX analysis confirmed heat-induced chemical and elemental changes in all tested sealers. Elevated temperature affects the physicochemical properties and structure of the tested endodontic sealers. The consideration of endodontic sealer compatibility is essential when warm gutta-percha obturation techniques are used.

Antimicrobial Activity of Five Calcium Silicate Based Root Canal Sealers against a Multispecies Engineered Biofilm: An In Vitro Study.

AIM: The present study's objective was to compare the impact of CerasealR, total fill BC SealerR, Bio-C SealerR, AH Plus BioceramicR, and K-BiocerR on the elimination of a multispecies' endodontic biofilm at 3, 7 and 14 days. MATERIALS AND METHODS: A total of 20 freshly extracted, caries-free premolars were prepared for the study to create dentinal disks. For the multispecies biofilm formation, Enterococcus faecalis, Proteus mirabilis, Pseudomonas aeruginosa, and Candida albicans were cultured and used to inoculate hydroxyapatite discs. After incubation, the biofilms were placed on blotting papers in petri dishes with an orthodontic bend. Different root canal sealers, including CeraSeal, total Fill BC Sealer, Bio-C Sealer, AH Plus Bioceramic, K-Biocer, and Sealite, were injected into the bend, facilitating contact with the biofilms. The samples were divided into seven groups, including a negative control. At specific intervals, 3, 7, and 14 days, 3 biofilm samples from each group were collected, diluted, and plated on Agar media for colony counting and analysis. RESULTS: In all tested groups, the total bacterial count significantly decreased between day 3 and 14 (p < 0.05) with no statistically significant differences among the different sealers' groups at all-time points for the total bacterial count, E. faecalis count, and P. mirabilis count. However, Sealite demonstrated the most consistent effectiveness in reducing bacterial counts across multiple categories. The sealite group was capable of decreasing the C. albicans count significantly between day 3 and day 14 (p < 0.05) in comparison with the bioceramic groups. CONCLUSION: All sealers had antibacterial activity against the multispecies biofilm between day 3 and day 14. The ascending order of sealers in terms of their effectiveness in killing bacteria, based on the provided results, is as follows: Sealite, Bio-C Sealer, AH Plus, CeraSeal, TotalFill, and K-Biocer. However, there were no statistically significant differences in the bacterial counts among the different sealer groups at any time point. CLINICAL SIGNIFICANCE: The role of sealers in combating biofilm-associated infections highlights their potential clinical utility in preserving root canal health. Understanding the antimicrobial properties of these sealers is vital for informed decision-making in selecting the most effective materials for improved treatment outcomes and long-term success in endodontic procedures.

Lack of Correlation Between the Penetration of Two Types of Sealers and Interfacial Adaptation to Root Dentine.

OBJECTIVE: This study aimed to determine the correlation between sealer penetration into dentinal tubules and interfacial adaptation to root canal walls using a hydraulic calcium silicate-based sealer, Endosequence Bioceramic Sealer (Brasseler USA, Savannah, GA, USA), and an epoxy resin-based sealer, AH Plus (Dentsply DeTrey, Konstanz, Germany). METHODS: Sixty-four maxillary central incisors were endodontically prepared with nickel-titanium rotary instruments and randomly assigned into two groups (n=32). Roots were filled with gutta-percha using a single-cone technique in conjunction with one of the two sealers, AHP or BCS. Sealers were mixed with Rhodamine B and analysed under a confocal laser scanning microscope. Transverse sections at 5 mm from root apex were obtained. The circumference of the root canal wall was first outlined and measured to determine the circumferential percentage of sealer penetration. The regions along the canal walls where the sealer had penetrated the dentinal tubules were delineated and measured. Then, the outlined distances were divided by the canal circumference. The width of each gap was measured and pooled for each specimen for comparison to determine the interfacial adaptation. The measurements were repeated twice to ensure reproducibility. Mann-Whitney tests were performed to compare continuous variables between AHP and BCS groups. The correlation between gap width and percentage of sealer penetration was investigated using the Spearman correlation coefficient. RESULTS: No significant difference was observed between groups regarding the percentage of sealer penetration (P>0.05) and the gap width (P>0.05). Also, there was no significant correlation between the two variables analysed for AHP (r=0.165; P>0.05) and BCS (r=-0.147; P>0.05) and in the overall sample (r=0.061; P>0.05). CONCLUSION: The present results show no correlation between interfacial adaptation and sealer penetration in dentinal tubules in the total sample and among sealer subgroups. The ability of root canal sealers to penetrate dentinal tubules cannot be considered a sign of better interfacial adaptation.

Effects of ultrasonic refinement on endodontic access cavity walls: A microcomputed tomography analysis.

OBJECTIVES: The present study aimed in assessing the coronal defects after access cavity finishing and refinement by micro. METHODS: Access cavities on thirty molars were prepared using a diamond bur. To finish and refine the access cavity, the Endo-Z was used in group 1 (n=15) and Start X 1 in group 2. Preparation time was recorded. A micro-CT scan was done before and after access preparation. Formation and location of the new defects were registered, the extension of defects calculated and the direction of the extension registered, preparation time and surface roughness determined (P < 0.05). RESULTS: Preparation time was significantly higher with ultrasonics (P <0.001). Internal walls showed smoother surfaces for Endo-Z group. Newly counts and extension length of defects weren't significantly different between groups (P > .05). CONCLUSION: Ultrasonic tips induced new cracks. Both instruments caused the extension of cracks. Ultrasonic tips requires more time and results in significantly rougher surfaces.

Impact of Warm Vertical Compaction on the Sealing Ability of Calcium Silicate-Based Sealers: A Confocal Microscopic Evaluation.

The aim of this in vitro study was to evaluate the dentinal tubule penetration of two calcium silicate-based sealers used in warm vertical compaction (WVC) obturation technique in comparison with the single cone (SC) technique by confocal laser scanning microscopy (CLSM). The null hypothesis was that both obturation techniques produced similar sealer penetration depths at 1 and 5 mm from the apex. Forty-four mandibular single-rooted premolars were randomly divided into four equally experimental groups (n = 10) and two control groups (n = 2) according to the type of sealer (Bio-C Angelus, Londrína, PR, Brazil or HiFlow Brasseler, Savannah, GA, USA) with either SC or WVC. The sealers were mixed with a fluorescent dye Rhodamine B (0.1%) to enable the assessment under the CLSM. All the specimens were sectioned horizontally at 1 and 5 mm from the apex. The maximum penetration depth was calculated using the ImageJ Software (ImageJ, NIH). Data were analyzed by Mann-Whitney U and Kruskal-Wallis tests (p < 0.05). A significant difference was shown between the four groups at 1 mm (p = 0.0116), whereas similar results were observed at 5 mm (p = 0.20). WVC allowed better diffusion for both sealers at 1 mm (p = 0.01) and 5 mm (p = 0.034). The maximum penetration of the Bio-C and HiFlow sealers was more important at 5 mm with the two obturation techniques. Within the limitations of this study, WVC enhanced the penetration of calcium silicate-based sealers into the dentinal tubules in comparison with the SC technique at both levels.

Assessment of root canal transportation of 2Shape and ProTaper gold in mandibular molar mesial canals: A micro-computed tomographic study.

The aim of this study was to compare shaping abilities of Protaper Gold® (PTG) and 2Shape® (TS) by using a new automatic process and micro-computed tomography (Micro-CT). 32 first mandibular molars with two separate mesial canals were selected. Only mesial roots were prepared with PTG and TS. Pre- and post-operative scans were performed using Micro-CT to provide volumes with a voxel size of 20 µm. Volumes, non-instrumented area, amount of transportation and centering ability in coronal, middle and apical third shaping time and procedural errors were recorded. TS and PTG increased the endodontic volume of 2.98 mm3 (±1.56) and 3.21 mm3 (±1.78) respectively with no statistical difference (p = .168) and no procedural errors. No significant difference was found concerning canal transportation among groups but only within the same group PTG (p value < .001) and TS (p value < .001). The mean centering ratio was significantly different only between the section levels for PTG (p value < .001) and TS (p value = .01); it was significantly reduced in the cervical third. The percentage of untouched canal walls ranged between 29.78% (±15.145) and 36.60% (±11.968) respectively for PTG and TS with no statistical difference among groups (p value = .168). TS and PTG with post machining heat treatment were able to produce centered preparations with no significant difference or procedural errors. TS system provided a shorter preparation time than PTG files.

Canal Transportation and Centring Ability of Reciproc and Reciproc Blue With or Without Use of Glide Path Instruments: A CBCT Study.

Objective: The objective of this ex vivo study was to evaluate canal transportation and centring ability of Reciproc and Reciproc blue systems in curved root canals with or without prior use of PathFile rotary system (PF) using Cone Beam Computed Tomography (CBCT). Methods: One hundred and twenty curved root canals from maxillary and mandibular premolars were selected. Canals were divided randomly into 4 groups (n=30): Reciproc 25 (R25), (PF+R25), Reciproc Blue 25 (RB25), (PF+RB 25). Specimens were scanned before and after root canal preparation. Using CBCT, root canal transportation and centring ability was assessed by measuring the shortest distance from the edge of uninstrumented canal to the periphery of the root (mesial and distal) before and after preparation. Data were analysed using a one-way analysis of variance and Tukey test. The p value was set at 0.05. Results: Less transportation and better centring ability occurred when PF was used before R25 or RB25 (P<0.0001). There was no significant difference between R25 and RB25 groups. Conclusion: Using PF before R25 and RB25 resulted in less root canal transportation and better centring ability. The specific thermo-mechanical treatment of RB25 did not provide better results when compared to R25.

Effect of 2 Heat-treated Nickel-Titanium Files on Enlargement and Deformation of the Apical Foramen in Curved Canals: A Scanning Electronic Microscopic Study.

INTRODUCTION: The aim of this study was to evaluate under scanning electronic microscopy the enlargement and deformation of the apical foramen of curved mesiobuccal roots with both reciprocating and rotary single-file systems made of a newly heat-treated alloy through the use of Reciproc Blue (REC Blue; VDW, Munich, Germany) and XP-endo Shaper (XPS; FKG Dentaire, La Chaux-de-Fonds, Switzerland). METHODS: Forty mesiobuccal roots of mandibular and maxillary molars presenting a curvature ranging from 20°-40° were used in this study. The apical foramens were initially scanned with a scanning electronic microscope under 200× magnification. Roots were divided into 2 groups (n = 20): the REC Blue group instrumented with the REC Blue system and the XPS group instrumented with the XPS system. Both groups were instrumented at the foramen. The apical foramens were scanned after root canal preparation. The area, circularity, and ratio of the Feret diameters were calculated from each image. The Kolmogorov-Smirnov, Mann-Whitney, and Wilcoxon tests were used for statistical analysis (P ≤ .05). RESULTS: The area of the foramen increased significantly after canal shaping with REC Blue and XPS. The variation of the area was significantly greater with XPS. However, there were no significant differences in circularity and the ratio of the Feret diameters with both instruments. CONCLUSIONS: No transportation was found when using REC Blue and XPS. The newly heat-treated nickel-titanium alloy did not promote deformation of the apical foramen in curved canals.

Anatomical Investigation of Middle Mesial Canals of Mandibular Molars in a Middle Eastern Population: A Cross-sectional Cone-beam Computed Tomography Study.

AIM: To identify the prevalence of middle mesial canals (MMC) in mandibular molars in a Lebanese population to determine the relationship between MMC and different factors (age, sex, and tooth type) and to examine the canal's morphological aspects (category, length, orifice position, and dentin width toward furcation). MATERIALS AND METHODS: The presence of MMC in the mesial roots of 505 mandibular molars of 200 patients was analyzed using cone-beam computed tomography (CBCT). Then, the position of the MMC orifices with respect to the pulpal floor and the main canals orifices, and the width of dentin along the canal toward the furcation were determined using 3D Slicer 4.10.1. RESULTS: In all, 14.65% of the first and second mandibular molars presented an MMC. A higher frequency of confluent canals was noted mostly joining the mesiobuccal canal (MBC). Gender appears to be a factor influencing the prevalence of MMCs, contrary to age-groups and tooth type. In mandibular first molars, the orifice is located at a mean distance of 1.22 ± 0.44 mm from the pulpal floor, 1.42 ± 0.53 mm from the MBC orifice, and 1.57 ± 0.60 mm from the mesiolingual canal (MLC) orifice. The width of dentin toward the furcation varies between 0.95 and 2.29 mm. In mandibular second molars, the orifice is located at a mean distance of 1.00 ± 0.51 mm from the pulpal floor, 1.39 ± 0.60 mm from the MBC orifice, and 1.37 ± 0.50 mm from MLC orifice. The width of dentin toward the furcation varies between 0.71 mm and 2.22 mm. CONCLUSION: Middle mesial canal is present in 14.65% of mandibular molars in the Lebanese population, with its orifice located under the pulpal floor. The majority of MMCs join the MBC. CLINICAL SIGNIFICANCE: Middle mesial canal is not a rare finding in the Lebanese population (14.65%). Clinicians should take time to search for this canal in the isthmus between the main mesial canals.

Post-endodontic Flare-ups after a Single-visit Treatment Using the FUI Scoring Method and Associated Factors: A Clinical Prospective Study.

AIM: Flare-ups following a root canal treatment are still a major problem both to the patient and to the clinician. Its definition, etiology, incidence, and risk factors have been the subject of long-standing debate. We conducted this study to investigate the incidence of flare-ups after a single-visit treatment and to identify the factors associated with the flare-up index (FUI). MATERIALS AND METHODS: All patients treated by postgraduate residents in endodontic specialty at Saint Joseph University of Beirut from June 2018 to January 2019 were invited to participate in the study. After a routine root canal treatment, patients were given a questionnaire to fill after 24 hours-48 hours-7 days, covering a postoperative assessment of symptoms using the visual analogue scale (VAS) and FUI. Patients who experienced a severe postoperative pain and/or swelling were identified and categorized as having a flare-up. RESULTS: A total of 423 patients (age: 39.76 ± 12.428 years) were included in the study. The incidence of flare-up was 1.9%. The mean FUI was 5.94 ± 5.646 and was highly correlated with the VAS score (p < 0.001). The factors that significantly influenced the occurrence of flare-ups were: pulpal diagnosis (p < 0.001), preoperative drug intake (p < 0.001), preoperative symptoms (>24 hours) before treatment (p < 0.001), and tooth type (p = 0.013). CONCLUSION: FUI should be used in further studies to confirm our results since it is a valid quantitative method to assess this clinical phenomenon. CLINICAL RELEVANCE: Endodontists should take into consideration the diagnosis and the history of preoperative symptoms since it plays an important role in predicting the occurrence of flare-ups and the success of the treatment.

Management of an Immature Necrotic Permanent Molar with Apical Periodontitis Treated by Regenerative Endodontic Protocol Using Calcium Hydroxide and MM-MTA: A Case Report with Two Years Follow Up.

Traditionally, immature teeth diagnosed with necrotic pulp and periapical periodontitis were treated by apexification with long-term calcium hydroxide or in one session with mineral trioxide aggregate (MTA) or Biodentine apical plug. However, these teeth become fragile and susceptible to root fracture. Regenerative endodontic procedure is a new therapeutic approach that promotes continuation of root growth in immature necrotic teeth potentially preventing root fracture. Only few case reports have shown the success of this procedure on molar cases. The current case report demonstrates a regeneration of a lower first molar with necrotic pulp and chronic apical abscess treated with Micro Mega-MTA (MM-MTA), a new endodontic biomaterial that has not been described previously. Calcium hydroxide was used as an intracanal medicament for two weeks. Next, calcium hydroxide was removed and after blood clot creation, MM-MTA® was placed over it. Apical healing and continuation of root growth were evident at nine months follow-up. CBCT at two years follow-up confirmed apical closure and complete healing. This case shows that a regenerative endodontic procedure for management of an immature necrotic permanent molar is feasible and can be successfully done using Ca(OH)2 and MM-MTA.

Dentinal tubule penetration of AH Plus, BC Sealer and a novel tricalcium silicate sealer: a confocal laser scanning microscopy study.

OBJECTIVES: The aim of this in vitro study was to assess the dentinal tubule penetration of three different sealers, AH Plus, BC Sealer and a novel tricalcium silicate sealer (NTS). MATERIALS AND METHODS: Ninety-six human maxillary central incisors were divided into three experimental groups (n = 32) and were filled with gutta-percha using a single-cone technique in conjunction with one of the three sealers: AH Plus, BC Sealer or NTS. The roots in each group were cross-sectioned at 1 and 5 mm from the root apex, and the surfaces were examined under confocal laser scanning microscopy (CLSM). The sealer penetration depths were measured at their maximum depths and at four circumferential depths (12, 3, 6 and 9 o'clock) and were evaluated using ImageJ software (ImageJ, NIH). RESULTS: The maximum and mean penetration depths were significantly higher at 5 mm compared to 1 mm from the apex in the AH Plus (p < 0.001), BC Sealer (p < 0.001) and NTS groups (p < 0.001). No significant difference was observed between the groups at 1 mm for both parameters. The maximum and mean penetration depths were significantly lower at 5 mm for AH Plus compared with the other two groups (p = 0.012). CONCLUSIONS: Within the study limitations, the BC Sealer and NTS demonstrated better tubule penetration results than the AH Plus sealer. CLINICAL RELEVANCE: Although no study has confirmed a relationship between the penetration depth of root canal sealers and the prevention of apical periodontitis, dentinal tubule sealer penetration may improve obturation quality.

Influence of the EndoActivator Irrigation System on Dentinal Tubule Penetration of a Novel Tricalcium Silicate-Based Sealer.

This study compared the effects of a conventional endodontic needle with an agitation system on a novel tricalcium silicate-based sealer (NTS) in terms of dentinal tubule penetration and interfacial adaptation to a root canal. Fifty single-rooted, recently-extracted human maxillary central incisors were randomly distributed into two homogeneous groups characterized by two different final cleansing systems: Conventional endodontic needle, or EndoActivator®. After instrumentation, all the teeth were filled with the gutta-percha single cone technique in conjunction with the novel tricalcium silicate-based sealer. Teeth were horizontally sectioned at 1 and 5 mm from the apex and were observed under a confocal laser scanning microscope (CLSM) at five magnifications. The maximum, mean, and the circumferential percentage of the sealer penetration inside the tubules were measured. Moreover, the gap width was evaluated using Image J software (National Institutes of Health, Bethesda, MD). EndoActivator® did not result in a significantly higher circumferential percentage of sealer penetration than conventional irrigation (p > 0.05). However, the gap width was significantly lower with EndoActivator®, compared to conventional needles at both 1 mm (p = 0.035) and 5 mm (p = 0.038). The EndoActivator® irrigation system did not significantly improve the NTS penetration, as compared to the conventional endodontic needle irrigation. Activation of the irrigation reduced the gap width significantly.

Impact of Minimal Root Canal Taper on the Fracture Resistance of Endodontically Treated Bicuspids.

OBJECTIVE: Minimal invasive endodontics preserve coronal and radicular tooth structure to increase the fracture resistance of teeth. The aim of this study was to assess the influence of final preparation taper on the fracture resistance of maxillary premolars. MATERIALS AND METHODS: Sixty maxillary premolars were selected and divided into 2 groups: 30 were shaped with a final apical diameter 30 and a 4% taper and 30 with 6% taper using iRaCe® instrument (FKG dentaire, Switzerland). All root canals were irrigated with sodium hypochlorite and final rinse with ethylenediaminetetraacetic acid. All canals were filled with gutta-percha single-cone filling technique and AHPlus® sealer (Dentsply- Maillefer, Baillagues, Switzerland) and access cavity restored with resin composite. Roots were wax coated, placed in an acrylic mold and loaded to compressive strength fracture in a mechanical material testing machine recording the maximum load at fracture and fracture pattern (favorable/restorable or unfavorable/unrestorable). Fracture loads were compared statistically, and data examined with Student t-test with a level of significance set at P ≤ 0.05. RESULTS: No statistically significant difference was registered between the 4% taper of preparation (270.47 ± 90.9 N) and 6% taper of preparation (244.73 ± 120.3 N) regarding the fracture resistance of the endodontically treated premolars tested (P = 0.541), while more favorable restorable fractures were registered in the 4% taper group. CONCLUSIONS: Continuous 4% preparation taper did not enhance the fracture resistance of endodontically treated maxillary premolars when compared to a 6% taper root canal preparation. More fractures were registered in the 4% taper group.

Cytotoxic evaluation of a new ceramic-based root canal sealer on human fibroblasts.

OBJECTIVES: The purpose of this study was to evaluate the cytotoxicity of a new bioceramic-based root canal sealer (BioMM) by direct and indirect contact with human fibroblasts and to compare it with a zinc oxide-eugenol sealer, the Pulp Canal Sealer-extended working time (PCS-EWT). MATERIALS AND METHODS: Cell viability was assessed through direct and indirect contact between human fibroblasts and sealer. Direct contact was performed at 24 h, whereas the indirect contact was performed at 24 and 48 h at different concentrations: 100%, 50%, and 25%. After direct contact, 3-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used and the optical density was measured by a spectrophotometer. Giemsa stain was also performed for a qualitative evaluation of the cells. STATISTICAL ANALYSIS USED: Shapiro-Wilk test was used to verify the normality of distribution of the variable. Data were analyzed by analysis of variance and Tukey tests using SPSS for Windows software 18.0. The significance level used was P < 0.05. RESULTS: Direct contact showed a significantly higher cell viability with BioMM as compared to PCS-EWT (P = 0.002). Cell viability at 24 h was significantly higher with BioMM compared to PCS-EWT for the concentrations of 50% (P = 0.004) and 25% (P = 0.003), whereas no significant difference was noted at 100% (P = 0.141). Cell viability at 48 h was significantly higher with BioMM as compared to PCS-EWT at 25% (P = 0.007). No significant difference was observed at 100% (P = 0.484) and 50% (P = 0.185). CONCLUSION: BioMM may be considered minimally cytotoxic if accidentally extruded into the periapical tissues.

Properties of Tricalcium Silicate Sealers.

INTRODUCTION: Sealers based on tricalcium silicate cement aim at an interaction of the sealer with the root canal wall, alkalinity with potential antimicrobial activity, and the ability to set in a wet field. The aim of this study was to characterize and investigate the properties of a new tricalcium silicate-based sealer and verify its compliance to ISO 6876 (2012). METHODS: A new tricalcium silicate-based sealer (Bio MM; St Joseph University, Beirut, Lebanon), BioRoot RCS (Septodont, St Maure de Fosses, France), and AH Plus (Dentsply, DeTrey, Konstanz, Germany) were investigated. Characterization using scanning electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction analysis was performed. Furthermore, sealer setting time, flow, film thickness, and radiopacity were performed following ISO specifications. pH and ion leaching in solution were assessed by pH analysis and inductively coupled plasma. RESULTS: Bio MM and BioRoot RCS were both composed of tricalcium silicate and tantalum oxide in Bio MM and zirconium oxide in BioRoot RCS. In addition, the Bio MM contained calcium carbonate and a phosphate phase. The inorganic components of AH Plus were calcium tungstate and zirconium oxide. AH Plus complied with the ISO norms for both flow and film thickness. BioRoot RCS and Bio MM exhibited a lower flow and a higher film thickness than that specified for sealer cements in ISO 6876. All test sealers exhibited adequate radiopacity. CONCLUSIONS: Bio MM interacted with physiologic solution, thus showing potential for bioactivity. Sealer properties were acceptable and comparable with other sealers available clinically.

MM-MTA for direct pulp capping: a histologic comparison with ProRoot MTA in rat molars.

AIM: To compare the histomorphological response of rat dental pulps capped with MM-MTA and ProRoot MTA at 1, 2 and 4 weeks postoperatively. MATERIALS AND METHODS: Direct pulp capping with MM-MTA and ProRoot MTA, overlaid with light-cured composite resin, was performed on right and left maxillary first molars of 20 Wistar rats. Animals were killed at 1, 2 and 4 weeks postoperatively. Biopsy samples were stained and viewed by light microscopy to determine dentin bridge formation. Data were statistically evaluated with the Kruskal-Wallis test (p < 0.05). RESULTS: Absence of dentin bridge formation at 1 week, partial dentin bridge formation at 2 weeks and complete dentin bridge formation at 4 weeks are observed with MM-MTA and ProRoot MTA. The results showed no statistically significant difference between both materials at 4 weeks. CONCLUSION: Both materials produced similar responses in the pulp when used for pulp capping in intact, caries-free molars of rats.