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This study presents a thorough investigation of the novel application of graphene oxide (GO) modified with melamine formaldehyde to fabricate granular three-dimensional GO (3D-GO), followed by the introduction of UiO-66 doping (3D-GO/U) for high uranium (U) adsorption. The U(VI) adsorption isotherms revealed that 3D-GO/U-10 with 10 % UiO-66 incorporation exhibited an impressive adsorption capacity of 375.5 mg g-1 and remained high U(VI) sorption performance in wide pH range. The introduction of UiO-66 to 3D-GO (3D-GO/U-10) led to the deagglomeration of the UiO-66 particles. The in situ surface-enhanced-Raman-spectroscopy-analysis and density-functional-theory simulations showed the symmetric metal center site Zr-O2 on UiO-66 was discovered to exhibit the highest adsorption energy (-3.21 eV) for U(VI) species due to the electrons transfer from the oxygen atom to U(VI) drives the covalent bonding between the symmetric metal center sites Zr-O2 and U(VI) on 3D-GO/U-10. The 3D-GO/U-10 was regenerated using a 0.1 M Na2CO3/0.01 M H2O2 solution and achieved up to 89.7 % U(VI) removal in the 5th cycle. The continuous flow column experiments results revealed 3D-GO/U-10 can regenerate and maintain a U(VI) removal capacity of â¼76 % for up to 4 cycles column experiments. Therefore, 3D-GO/U-10 exhibits great potential for removing U(VI) from water bodies.
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Solar-driven artificial photosynthesis offers a promising avenue for hydrogen peroxide (H2O2) generation, an efficient and economical replacement for current methods. The efficiency and selectivity hurdles of the two-electron oxygen reduction reaction (ORR) in solar-to- H2O2 conversion are substantial barriers to large scale production. In this manuscript, a simple biomass-assisted synthesis was performed to produce oxygen-enriched carbon quantum dots (OE-CQDs) from spent coffee waste, acting as an efficient photocatalyst for solar-powered H2O2 production. OE-CQDs can stabilize and store light-generated electrons effectively, boosting charge separation and enhancing photocatalytic performance with longevity. The maximal photocatalytic H2O2 production was achieved viz the utilization of OE-CQDs with generation rate of 356.86 µmol g-1 h-1 by retaining 80% activity without any external sacrificial donors. The outstanding performance of synthesized OE-CQDs under light exposure at wavelength (λ) of 280 nm has been ensured by the quantum yield value of 9.4% upon H2O2 generation. The combinatorial benefits of OE-CQDs with their authentic crystalline structure and oxygen enrichment, is expected to be enhancing the ORR activity through accelerating charge transfer, and optimizing oxygen diffusion. Consequently, our eco-friendly method holds considerable promise for creating highly efficient, metal-free photocatalysts for artificial H2O2 production.
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Carbono , Café , Peróxido de Hidrogênio , Oxigênio , Pontos Quânticos , Luz Solar , Pontos Quânticos/química , Oxigênio/química , Catálise , Peróxido de Hidrogênio/química , Carbono/química , Café/química , Oxirredução , Processos FotoquímicosRESUMO
Electrode-driven microbial electron transfer enables the conversion of CO2 into multi-carbon compounds. The electrosynthetic biofilms grow slowly on the surface and are highly susceptible to operational influences, such as hydrodynamic shear stress. In this study, a cylindrical roll-up carbon felt electrode was developed as a novel strategy to protect biofilms from shear stress within the reactor. The fabricated electrode allowed hydrogen bubble formation inside the structure, which enabled microbes to uptake hydrogen and convert CO2 to multi-carbon organic compounds. The roll-up electrode exhibited faster start-up and biofilm formation than the conventional linear shape carbon felt. The acetate yield and cathodic faradaic efficiency increased by 80% and 34%, respectively, and the bioelectrochemical stability was improved significantly. The roll-up structure increased biofilm development per unit electrode surface by three to five-fold. The roll-up configuration improved biofilm formation on the electrode, which enhanced the performance of microbial electrosynthesis-based CO2 valorization.
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Dióxido de Carbono , Carbono , Dióxido de Carbono/química , Habitação , Fibra de Carbono , Hidrogênio , Eletrodos , BiofilmesRESUMO
Acetate is an end-product of anaerobic biodegradation and one of the major metabolites of microbial fermentation and lingo-cellulosic hydrolysate. Recently, acetate has been highlighted as a feedstock to produce value-added chemicals. This study examined acetate conversion to succinate by citrate synthase (gltA)-overexpressed Pseudomonas putida under microaerobic conditions. The acetate metabolism is initiated with the gltA enzyme, which converts acetyl-CoA to citrate. gltA-overexpressing P. putida (gltA-KT) showed an â¼50% improvement in succinate production compared to the wild type. Under the optimal pH of 7.5, the accumulation of succinate (4.73 ± 0.6 mM in 36 h) was â¼400% higher than that of the wild type. Overall, gltA overexpression alone resulted in 9.5% of the maximum theoretical yield in a minimal medium with acetate as the sole carbon source. This result shows that citrate synthase is important in acetate conversion to succinate by P. putida under microaerobic conditions.
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The aim of this study was to investigate the effects of hydrophilic sulfur-modified nanoscale zero-valent iron (S-nZVI) as a biocatalyst for denitrification. We found that the denitrifying bacteria Cupriavidus necator (C. necator) promoted Fe corrosion during biocatalytic denitrification, reducing surface passivation and sulfur species leaching from S-nZVI. As a result, S-nZVI exhibited a higher synergistic factor (fsyn = 2.43) for biocatalytic NO3- removal than nanoscale zero-valent iron (nZVI, fsyn = 0.65) at an initial nitrate concentration of 25 mg L-1-N. Based on kinetic profiles, SO42- was the preferred electron acceptor over NO3- when using C. necator and S-nZVI for biocatalytic denitrification. Up-flow column experiments demonstrated that biocatalytic denitrification using S-nZVI achieved a total nitrogen removal capacity of up to 2004 mg L-1 for 127 d. Notably, microbiome taxonomic profiling showed that the addition of S-nZVI to the groundwater promoted the growth of Geobacter, Desulfosporosinus, Streptomyces, and Simplicispira spp in the column experiments. Most of those microbes can reduce sulfate, promote denitrification, and match the batch kinetic profile obtained using C. necator. Our results not only discover the great potential of S-nZVI as a biocatalyst for enhancing denitrification via microbial activation but also provide a deep understanding of the complicated abiotic-biotic interaction.
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Água Subterrânea , Poluentes Químicos da Água , Ferro , Desnitrificação , Nitratos , Bactérias , NitrogênioRESUMO
Effects of limited dissolved oxygen (DO) on hydrogenotrophic denitrification at biocathode was investigated using bioelectrochemical system. It was found that total nitrogen removal increased by 5.9%, as DO reached about 0.24 mg/L with the cathodic chamber unplugged (group R_Exposure). With the presence of limited DO, not only the nitrogen metabolic pathway was influenced, but the composition of microbial communities of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria were enriched accordingly. After metagenomic analysis, enriched genes in R_Exposure were found to be associated with nearly each of nitrogen removal steps as denitrification, nitrification, DNRA, nitrate assimilation and even nitrogen fixation. Moreover, genes encoding both Complexes III and IV constituted the electron transfer chain were significantly enriched, indicating that more electrons would be orientated to the reduction of NO2--N, NO-N and oxygen. Therefore, enhanced nitrogen removal could be attained through the co-respiration of nitrate and oxygen by means of NH4+-N oxidation.
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Desnitrificação , Nitratos , Nitratos/metabolismo , Nitrogênio/metabolismo , Oxigênio , Reatores Biológicos , Nitrificação , OxirreduçãoRESUMO
The interactions between the microbes and the surface of an anode play an important role in capturing the respiratory electrons from bacteria in a microbial fuel cell (MFC). The chemical and electrochemical characteristics of the carbon material affect biofilm growth and direct electron transfer in MFCs. This study examined the electrodeposition of polydopamine (PDA) and polypyrrole (PPY) on graphite felt electrode (GF). The MFC with the modified PDA/PPY-GF reached 920 mW/m2, which was 1.5, 1.17, and 1.18 times higher than those of the GF, PDA-GF, and PPY-GF, respectively. PDA has superior hydrophilicity and adhesive force biofilm formation, while PPY provides electrochemically active sites for microbial electron transfer. Raman spectroscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller surface area measurements, and contact angle analysis revealed the enhanced physicochemical properties of the carbon electrode. These results show that co-doped PDA/PPY provides a strategy for electroactive biofilm development and improves the bioelectrochemical performance in realistic MFC reactors.
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Fontes de Energia Bioelétrica , Grafite , Fontes de Energia Bioelétrica/microbiologia , Polímeros/química , Grafite/química , Pirróis/química , Bactérias , Carbono , EletrodosRESUMO
The inherent recalcitrance of lignocellulosic biomass is a significant barrier to efficient lignocellulosic biorefinery owing to its complex structure and the presence of inhibitory components, primarily lignin. Efficient biomass pretreatment strategies are crucial for fragmentation of lignocellulosic biocomponents, increasing the surface area and solubility of cellulose fibers, and removing or extracting lignin. Conventional pretreatment methods have several disadvantages, such as high operational costs, equipment corrosion, and the generation of toxic byproducts and effluents. In recent years, many emerging single-step, multi-step, and/or combined physicochemical pretreatment regimes have been developed, which are simpler in operation, more economical, and environmentally friendly. Furthermore, many of these combined physicochemical methods improve biomass bioaccessibility and effectively fractionate â¼96 % of lignocellulosic biocomponents into cellulose, hemicellulose, and lignin, thereby allowing for highly efficient lignocellulose bioconversion. This review critically discusses the emerging physicochemical pretreatment methods for efficient lignocellulose bioconversion for biofuel production to address the global energy crisis.
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Biocombustíveis , Lignina , Lignina/química , Biomassa , Celulose , HidróliseRESUMO
Premature drug release and poor controllability is a challenge in the practical application of tumor therapy, which may lead to poor chemotherapy efficacy and severe adverse effects. In this study, a reactive oxygen species (ROS)-cleavable nanoparticle system (MXene-TK-DOX@PDA) was designed for effective chemotherapy drug delivery and antibacterial applications. Doxorubicin (DOX) was conjugated to the surface of (3-aminopropyl)triethoxysilane (APTES)-functionalized MXene via an ROS-cleavable diacetoxyl thioketal (TK) linkage. Subsequently, the surfaces of the MXene nanosheets were coated with pH-responsive polydopamine (PDA) as a gatekeeper. PDA endowed the MXene-TK-DOX@PDA nanoparticles with superior biocompatibility and stability. The MXene-TK-DOX@PDA nanoparticles had an ultrathin planar structure and a small lateral size of approximately 180 nm. The as-synthesized nanoparticles demonstrated outstanding photothermal conversion efficiency, superior photothermal stability, and a remarkable extinction coefficient (23.3 L g-1 cm-1 at 808 nm). DOX exhibited both efficient ROS-responsive and pH-responsive release performance from MXene-TK-DOX@PDA nanoparticles due to the cleavage of the thioketal linker. In addition, MXene-TK-DOX@PDA nanoparticles displayed high antibacterial activity against both Gram-negative Escherichia coli (E. coli) and Gram-positive Bacillus subtilis (B. subtilis) within 5 h. Taken together, we hope that MXene-TK-DOX@PDA nanoparticles will enrich the drug delivery system and significantly expand their applications in the biomedical field.
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In this study, a novel MXene (Ti3C2Tx)-based nanocarrier was developed for drug delivery. MXene nanosheets were functionalized with 3, 3'-diselanediyldipropionic acid (DSeDPA), followed by grafting doxorubicin (DOX) as a model drug to the surface of functionalized MXene nanosheets (MXene-Se-DOX). The nanosheets were characterized using scanning electron microscopy, atomic force microscopy (AFM), transmission electron microscopy, energy-dispersive X-ray spectroscopy (EDX), nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and zeta potential techniques. The drug-loading capacity (17.95%) and encapsulation efficiency (41.66%) were determined using ultraviolet-visible spectroscopy. The lateral size and thickness of the MXene nanosheets measured using AFM were 200 nm and 1.5 nm, respectively. The drug release behavior of the MXene-Se-DOX nanosheets was evaluated under different medium conditions, and the nanosheets demonstrated outstanding dual (reactive oxygen species (ROS)- and pH-) responsive properties. Furthermore, the MXene-Se-DOX nanosheets exhibited excellent antibacterial activity against both Gram-negative E. coli and Gram-positive B. subtilis.
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Sistemas de Liberação de Medicamentos , Escherichia coli , Doxorrubicina/farmacologia , Doxorrubicina/química , Antibacterianos/farmacologia , Antibacterianos/química , Liberação Controlada de Fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Concentração de Íons de HidrogênioRESUMO
Chlorella is a promising microalga for CO2-neutral biorefinery that co-produces drop-in biofuels and multiple biochemicals. Cell disruption and selective lipid extraction steps are major technical bottlenecks in biorefinement because of the inherent robustness and complexity of algal cell walls. This review focuses on the state-of-the-art achievements in cell disruption and lipid extraction methods for Chlorella species within the last five years. Various chemical, physical, and biological approaches have been detailed theoretically, compared, and discussed in terms of the degree of cell wall disruption, lipid extractability, chemical toxicity, cost-effectiveness, energy use, scalability, customer preferences, environment friendliness, and synergistic combinations of different methods. Future challenges and prospects of environmental-friendly and efficient extraction technologies are also outlined for practical applications in sustainable Chlorella biorefineries. Given the diverse industrial applications of Chlorella, this review may provide useful information for downstream processing of the advanced biorefineries of other algae genera.
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Chlorella , Microalgas , Biocombustíveis , Parede Celular , Lipídeos , BiomassaRESUMO
Acetate is a major byproduct of the bioconversion of the greenhouse gas carbon dioxide, pretreatment of lignocellulose biomass, and microbial fermentation. The utilization and valorization of acetate have been emphasized in transforming waste to clean energy and value-added platform chemicals, contributing to the development of a closed carbon loop toward a low-carbon circular bio-economy. Acetate has been used to produce several platform chemicals, including succinate, 3-hydroxypropionate, and itaconic acid, highlighting the potential of acetate to synthesize many biochemicals and biofuels. On the other hand, the yields and titers have not reached the theoretical maximum. Recently, recombinant strain development and pathway regulation have been suggested to overcome this limitation. This review provides insights into the important constraints limiting the yields and titers of the biochemical and metabolic pathways of bacteria capable of metabolizing acetate for acetate bioconversion. The current developments in recombinant strain engineering are also discussed.
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Renewable electricity-based microbial electrosynthesis can upgrade CO2 into value-added chemicals and simultaneously increase the number of biocatalysts by cell growth, helping to achieve sustainable carbon-negative processes. In most studies, the main strategy for improving the MES performance was to enhance H2-based electron uptake by decreasing the overpotential and electrical conductivity of the electrode. Less is known about the electrode-based direct electron uptake for CO2 conversion in MES. In this study, a solid neutral red/Nafion conductive layer was developed on the carbon electrode surface using a feasible dip and dry method. The modified electrode showed higher HER overpotential and lower capacitance but enhanced redox capability and hydrophobicity, which increased direct electron transport to the bacteria rather than hydrogen-based indirect electron delivery. The Neutral red/Nafion-implemented MES showed faster start-up, higher acetate production, and energy efficiency than the non-modified electrode.
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Dióxido de Carbono , Carbono , Acetatos/metabolismo , Dióxido de Carbono/metabolismo , Fibra de Carbono , Conservação de Recursos Energéticos , Condutividade Elétrica , Eletrodos , Polímeros de Fluorcarboneto , Hidrogênio , Vermelho NeutroRESUMO
Translocation of cell-penetrating peptides is promoted by incorporated arginine or other guanidinium groups. However, relatively little research has considered the role of these functional groups on antimicrobial peptide activity. A series of cationic linear-, star- and multi-branched-poly(L-arginine-co-L-phenylalanine) have been synthesized via the ring-opening copolymerizations of corresponding N-carboxyanhydride monomers followed by further modifications using the N-heterocyclic carbene organocatalyst. All the polymers are characterized by the random coiled microstructure. Antibacterial efficacy, tested by the gram-positive B. subtilis bacteria and the gram-negative E. coli bacteria, was sensitive to the structure and relative composition of the copolymer and increased in the order of linear- < star- < multi-branched structure. The multi-branched-p[(L-arginine)23-co-(L-phenylalanine)7]8 polymer showed the best antibacterial property with the lowest minimum inhibitory concentration values of 48 µg mL−1 for E. coli and 32 µg mL−1 for B. subtilis. The efficacy was prominent for B. subtilis due to the anionic nature of its membrane. All of the resultant arginine moiety-containing polypeptides showed excellent blood compatibility. The antibiotic effect of the copolymers with arginine moieties was retained even in the environment bearing Ca2+, Mg2+, and Na+ ions similar to blood plasma. The cationic arginine-bearing copolypeptides were also effective for the sterilization of naturally occurring sources of water such as lakes, seas, rain, and sewage, showing a promising range of applicability.
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This study reports for the first-time the possibility of deploying gas production by thiosulfate utilizing denitrifying bacteria (TUDB) as a proxy to evaluate water toxicity. The test relies on gas production by TUDB due to inhibited metabolic activity in the presence of toxicants. Gas production was measured using a bubble-type respirometer. Optimization studies indicated that 300 mg NO3--N/L, 0.5 mL acclimated culture, and 2100 mg S2O32-/L were the ideal conditions facilitating the necessary volume of gas production for sensitive data generation. Determined EC50 values of the selected heavy metals were: Cr6+, 0.51 mg/L; Ag+, 2.90 mg/L; Cu2+, 2.90 mg/L; Ni2+, 3.60 mg/L; As3+, 4.10 mg/L; Cd2+, 5.56 mg/L; Hg2+, 8.06 mg/L; and Pb2+, 19.3 mg/L. The advantages of this method include operational simplicity through the elimination of cumbersome preprocessing procedures which are used to eliminate interferences caused by turbidity when the toxicity of turbid samples is determined via spectrophotometry.
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Metais Pesados , Poluentes Químicos da Água , Bactérias , Bioensaio , Monitoramento Ambiental/métodos , Metais Pesados/análise , Metais Pesados/toxicidade , Tiossulfatos , Água , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Pseudomonas strains are a promising host cell in metabolic engineering for bioconversion, environmental remediation, and most recently for bioelectrochemical applications. This study isolated an electrochemically active Pseudomonas sp. from an anaerobic sludge using a colorimetric and electrochromic WO3 nanorod (WO3-NR) probe. A strategy was developed to determine the presence of electroactive species from enriched cultures. A mixed consortium was enriched using Pseudomonas isolation media containing betaine and triclosan as the carbon source and antibacterial reagent, respectively. A single blue colony was isolated using WO3-NR sandwiched agar plates. The isolate was sequenced by 16 s rRNA and designated Pseudomonas aeruginosa PBH03, producing phenazines and pyocyanin aerobically. The isolate exhibited clear electrochemical characteristics from cyclic voltammetry and linear sweep voltammetry and produced a current density of 9.01 µA cm-2 in a microbial fuel cell.
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Nanotubos , Tungstênio , Colorimetria , Pseudomonas , Pseudomonas aeruginosa/metabolismo , Piocianina/metabolismo , Tungstênio/metabolismoRESUMO
As a new approach of creating the photo-exited electron (e-) and hole (h+) mediation zone for highly selective singlet oxygen (1O2) production, the rod-type graphitic carbon nitride (NCN) has been synthesized from the nitric acid-modified melamine followed by the calcination. The NCN exhibited a higher surface area and surface oxygen adsorption ability than bulk graphitic carbon nitride (BCN). The increment of CO and NHx groups on NCN corresponded to e- and h+ mediation groups, respectively, resulting in higher production of 1O2 than BCN. Moreover, those mediation groups on NCN result in higher recombination efficiency and longer e- decay time. As a result, the optimized NCN-0.5 (derived from 0.5â¯M of nitric acid-modified melamine) displayed 5.8 times higher kinetic rate constant of atrazine (ATZ) removal under UVA-LED irradiation compared to BCN. This study also evaluated the ATZ degradation pathways and toxicity effect of by-products. In addition, continuous flow experiments using NCN-0.5 showed superior ATZ removal performance with a hybrid concept between a slurry photocatalysis and a continuous stirred tank reactor system using actual effluent obtained from a wastewater treatment plant. Thus, this work provides an insight into the strategy for highly selective 1O2 production and the potential for water purification application.
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Electroactive microorganisms acting as microbial electrocatalysts have intrinsic metabolisms that mediate a redox potential difference between solid electrodes and microbes, leading to spontaneous electron transfer to the electrode (exo-electron transfer) or electron uptake from the electrode (endo-electron transfer). These microbes biochemically convert various organic and/or inorganic compounds to electricity and/or biochemicals in bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs) and microbial electrosynthesis cells (MECs). For the past two decades, intense studies have converged to clarify electron transfer mechanisms of electroactive microbes in BESs, which thereby have led to improved bioelectrochemical performance. Also, many novel exoelectrogenic eukaryotes as well as prokaryotes with electroactive properties are being continuously discovered. This review presents an overview of electroactive microorganisms (bacteria, microalgae and fungi) and their exo- and endo-electron transfer mechanisms in BESs for optimizing and advancing bioelectrochemical techniques.
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Fontes de Energia Bioelétrica , Elétrons , Eletroquímica , Eletrodos , Transporte de ElétronsRESUMO
A class of phenolphthalein anilide (PA)-based poly(ether sulfone) multiblock copolymers containing pendant quaternary ammonium (QA) and imidazolium (IM) groups were synthesized and evaluated as anion exchange membrane (AEM) materials. The AEMs were flexible and mechanically strong with good thermal stability. The ionomeric multiblock copolymer AEMs exhibited well-defined hydrophobic/hydrophilic phase-separated morphology in small-angle X-ray scattering and atomic force microscopy. The distinct nanophase separated membrane morphology in the AEMs resulted in higher conductivity (IECw = 1.3-1.5 mequiv./g, σ(OH-) = 30-38 mS/cm at 20 °C), lower water uptake and swelling. Finally, the membranes were compared in terms of microbial fuel cell performances with the commercial cation and anion exchange membranes. The membranes showed a maximum power density of ~310 mW/m2 (at 0.82 A/m2); 1.7 and 2.8 times higher than the Nafion 117 and FAB-PK-130 membranes, respectively. These results demonstrated that the synthesized AEMs were superior to Nafion 117 and FAB-PK-130 membranes.
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Bioelectrochemical system (BES) is a promising solution for mitigation of ammonia inhibition in anaerobic digestion (AD) process. However, the effect of electric field intensity on microbial community changes and metabolic function prediction during the alleviation of ammonia inhibition are still missing. The results of the current study represented that the improvement of ammonia removal (20.6%) and methane production (14.6%) could both be achieved at 0.2 V while higher voltages led to reductions of methane production (more than 48.9%) compared with the control. Moreover, hydrogenotrophic methanogens (Methanobacterium) seemed to be more robust to high voltages compared with aceticlastic methanogens (Methanosaeta). Additionally, bacteria for hydrolysis and acidogenesis (Rikenellaceae and Soehngenia) were found vulnerable to external electric field intensity. Furthermore, abundances changes of metabolic pathways demonstrated that the degradation of carbohydrates, lipids and proteins during all steps (hydrolysis, acidogenesis, acetogenesis and methanogenesis) of AD process could be affected by different applied voltages.