A novel macrolactam-trisaccharide antifungal antibiotic. Taxonomy, fermentation, isolation, physico-chemical properties, structure elucidation and biological activity.

A novel secondary metabolite SCH 42282 (1), with antifungal activity was isolated from the fermentation broth of a soil actinomycete identified as a Microtetraspora sp. The active compound was separated from the fermentation broth by butanol extraction and purified on a silica gel column and by multi-coil counter current chromatography. The compound was identified as a novel macrolactam trisaccharide related to SCH 38518 (4). The structure was established by hydrolysis of the parent compound and spectroscopic studies of the acetate derivative. The compound is active against Candida spp. (Geometric Mean MIC's. 18 micrograms/ml) but less active SCH 42729 (3). the disaccharide (Geometric Mean MIC's, > or = 10.7 micrograms/ml and SCH 38518 (4), the monosaccharide (Geometric Mean Mic's, 3.8 micrograms/ml.

A novel macrolactam-disaccharide antifungal antibiotic. Taxonomy, fermentation, isolation, physico-chemical properties, structure elucidation and biological activity.

A novel natural product (1), with antifungal activity was isolated from the culture broth of an actinomadurae. The active compound was separated from broth by n-butanol extraction and purified by silica gel and multicoil counter current chromatography. Physico-chemical data suggested the structure of this compound to be a novel macrolactam disaccharide related to Sch 38518 (3). The structure was determined by spectroscopic studies on the acetate derivative. It was active against Candida spp. (MIC's, 4 approximately 64 micrograms/ml) but less than the monosaccharide, Sch 38518 (MIC's, 1 approximately 16 micrograms/ml).

Differential growth among components of the palate in rhesus monkeys (Macaca mulatta).

To understand the etiology of maxillary hypoplasia better, which is common in individuals with cleft palate and other craniofacial anomalies, the relative growth and contribution of the maxillary and palatine segments to enlargement of the hard palate was examined in rhesus monkeys. The purpose of the study was to identify and evaluate sites of differential growth of the palatine and maxillary segments as they contribute to the development of the midface and facial prognathism. One hundred and eight male and 107 female skulls ranging from 1.0 to 7.0 years of age were examined. Measurements of the maxillary and palatine lengths, palatal width, midface depth, canine length, and molar surface areas in the ontogenetic sample were collected. Univariate and bivariate statistics were used to describe dimensional changes and evaluate region-specific sex differences. The relative growth of palatal dimensions was evaluated using allometric analysis methods. Significant sex differences were observed (t-test, p < or = .05) for all palatal dimensions by 4 years of age. However, proportions of the maxillary and palatine segments to overall palate length appeared to be similar throughout growth for both sexes. The results suggest that sutures of the midface, in particular the transverse palatine suture, may be important in the bony development of the palate during growth. These sutures may contribute to the overall modulation of palatal development.(ABSTRACT TRUNCATED AT 250 WORDS)

SCH 45752--an inhibitor of calmodulin-sensitive cyclic nucleotide phosphodiesterase activity.

A highly potent inhibitor of calmodulin-sensitive phosphodiesterase (PDE) activity was isolated from the culture broth of an unidentified fungal isolate, SCF-125. A chemically defined medium was developed for production of this compound. The PDE inhibitor was isolated from the fermentation filtrate by adsorption on a macro-reticular resin and further purified by gel filtration chromatography and reverse-phase HPLC. The major PDE inhibitor was identified as cephalochromin, a bis-naphthopyrone, by spectral data analysis. The compound, SCH 45752, inhibited calmodulin-sensitive PDE activities with IC50 values of 40-47 nM. It inhibited the activities of calmodulin-independent PDE and various protein kinases with higher IC50 values (2-40 microM). SCH 45752 does not appear to be a calmodulin antagonist. Furthermore, SCH 45752 affects smooth muscle contraction at a concentration of 30 microM; it potentiated the relaxing effect of sodium nitroprusside on carotid artery media contracted by histamine. Thus SCH 45752 is one of the most potent inhibitors of calmodulin-sensitive PDE activity known, and it is capable of exerting a pharmacological effect in at least one intact tissue model.

Infection control of in-office dental laboratories.

The importance of observing a sound infection control program in the dental laboratory is clear. Personnel should not be expected to work in an unsafe environment. Many of the reasons for ignoring proper disinfection control procedures are unfounded. Infection control procedures do not adversely affect either laboratory personnel or dental prostheses when carefully executed.

Gastric bezoars: treatment and prevention.

Gastric bezoars may occur in the normal stomach as a result of ingestion of various objects which do not pass through the pylorus. Most gastric bezoars occur as a complication of previous gastric surgery in which there is a loss of normal pyloric function, hypoperistalsis, and low gastric acidity. They may also occur as a complication of cimetidine therapy. Symptoms include epigastric fullness, regurgitation, nausea and vomiting, and epigastric pain. A simple treatment utilizing an ordinary Teledyne Water Pik jet stream through a gastroscope is described to break up a large phytobezoar. This method is probably the treatment of choice and should be used more widely.