RESUMO
An enzyme immunoassay system has been developed for the detection of Ebola virus antigen. It permits a highly accurate and sensitive rapid detection of the antigen. Optimal dilutions of specific immunoglobulin (1:500, corresponding to protein concentration of 50 micrograms/ml) and conjugate were found. The resolving capacity of the new test system is 1.9 x 10(-7) g protein.
Assuntos
Antígenos Virais/análise , Ebolavirus/imunologia , Técnicas Imunoenzimáticas , Animais , Haplorrinos , Sensibilidade e EspecificidadeRESUMO
With the use of the unified indirect solid-phase fluorescent enzyme immunoassay the combined evaluation of the antigenic and immunogenic properties of experimental whole-virion inactivated virus vaccines against Venezuelan and eastern equine encephalomyelitides, as well as of dried chemical typhus vaccine, has been made; their safety was determined indirectly by the content of ovalbumin. The protective role of antibodies evaluated by the solid-phase fluorescent enzyme immunoassay in typhus immunity has been shown.
Assuntos
Antígenos de Bactérias/análise , Antígenos Virais/análise , Vírus da Encefalite Equina do Leste/imunologia , Vírus da Encefalite Equina Venezuelana/imunologia , Fluorimunoensaio/métodos , Rickettsia prowazekii/imunologia , Vacinas Antirrickéttsia/análise , Vacinas Virais/análise , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Epitopos/análise , Cobaias , Macaca fascicularis , Coelhos , Vacinas Antirrickéttsia/imunologia , Vacinas de Produtos Inativados/análise , Vacinas de Produtos Inativados/imunologia , Vacinas Virais/imunologiaRESUMO
A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.
Assuntos
Antígenos Virais/análise , Marburgvirus/imunologia , Animais , Ebolavirus/imunologia , Estudos de Avaliação como Assunto , Himecromona/análogos & derivados , Técnicas Imunoenzimáticas , Indicadores e Reagentes , Nitrofenóis , Compostos Organofosforados , CoelhosRESUMO
The blood sera of persons immunized with different typhus vaccines have been studied in the complement fixation test, the indirect hemagglutination test and the enzyme immunoassay. The data thus obtained indicate that the enzyme immunoassay is highly sensitive and can be universally used for the determination of antibodies to Rickettsia prowazekii after primary and booster immunization with different typhus vaccines. This method detects specific antibodies both at an early period and, which is of particular importance, at a remote period after immunization (3 years later) when complement-binding and hemagglutinating antibodies are absent. This is seemingly indicative of the two-phase character of postvaccinal immunity induced by live typhus vaccine.