[Colonization of cucumber rhizosphere by phosphate-mobilizing bacteria of Bacillus genus].

An ability of phosphate-mobilizing bacteria, related to four species of Bacillus genus, to colonize the cucumber root zone has been investigated. It has been found that all the studied strains can adapt to the root zone of those plants colonizing its parts to different extent. Essential nonuniformity of the root surface populating with bacteria is noted. Microcolonies of these organisms are formed in some root zones. This peculiarity is probably caused both by the difference in the number and composition of exudates of some zones of the plant roots, and by peculiarities of trophic requirements of the strains of studied bacteria The paper is presented in Ukrainian.

[Synthesis of immunomagnetic sorbents for separation of hepatitis B and C viruses].

This study was devoted to the creation of biomagnetic sorbents, capable of removing the virus particles from the human blood serum. With this purpose specific globulins were isolated from reconvalescents sera after acute hepatitis B virus (HBV) and hepatitis C virus (HCV), which were later used as molecules-vectors (ligands). Ligands were covalently bound to magnetite encapsulated by the matrix, carrying aminopropyl groups on its surface. The dynamics of immunoglobulin sorption with magnet controllable bearer was studied. Sera which were positive by DNA HBV, by RNA HCVin polymerase-chain reaction (PCR) and by surface antigen HBsAg in immunosorbent assay (ELISA) were approbated with the purpose of virus separation by immunomagnetic sorbents. Completed virus decontamination of the serum was achieved according to results of PCR on DNA HBV, at the same time the results of ELISA testify to the presence of HBsAg in the tested samples of the serum, it can be associated with the presence of many defect virus particles in population or with the lack of biomagnetic sorbent for complete elimination. Results ofquality PCR on RNA HCV of sera after decontamination testify to the absence of complete HCV elimination.

Structural and functional organisation of regenerated plant protoplasts exposed to microgravity on Biokosmos 9.

Preparatory experiments for the IML-1 mission using plant protoplasts, were flown on a 14-day flight on Biokosmos 9 in September 1989. Thirty-six hours before launch of the biosatellite, protoplasts were isolated from hypocotyl cells of rapeseed (Brassica napus) and suspension cultures of carrot (Daucus carota). Ultrastructural and fluorescence analysis of cell aggregates from these protoplasts, cultured under microgravity conditions, have been performed. In the flight samples as well as in the ground controls, a portion of the total number of protoplasts regenerated cell walls. The processes of cell differentiation and proliferation under micro-g did not differ significantly from those under normal gravity conditions. However, in micro-g differences were observed in the ultrastructure of some organelles such as plastids and mitochondria. There was also an increase in the frequency of the occurrence of folds formed by the plasmalemma together with an increase in the degree of complexity of these folds. In cell cultures developed under micro-g conditions, the calcium content tends to decrease, compared to the ground control. Different aspects of using isolated protoplasts for clarifying the mechanisms of biological effects of microgravity are discussed.

The effect of exposure to microgravity on the development and structural organisation of plant protoplasts flown on Biokosmos 9.

Preparatory experiments for the IML-1 (International Microgravity Laboratory) mission to be flown on the Space Shuttle in January, 1992, were performed on a 14 day flight on Biokosmos 9 (Kosmos 2044) in September 1989. The purpose of the experiment was to study the effect of weightlessness on protoplast regeneration. Problems with late access to the space vehicle meant that the newly isolated protoplasts from hypocotyl cells of rapeseed (Brassica napus L. cv Niklas) and suspension cultures of carrot (Daucus carota L, cv Nobo) had to be stored at 4 degrees C for 36 h prior to the launch of the biosatellite, in order to delay cell wall regeneration until the samples were in orbit. In the flight samples and the ground controls, a portion of the total number of protoplasts regenerated cell walls. The growth of flight rapeseed cells was only 56% compared to the ground control; the respective growth of carrot cells in orbit was 82% of the ground control. Analysis demonstrated that the peroxidase activity and the amount of protein was lower in the flight samples than in the ground controls. The number of different isoenzymes was also decreased in the flight samples. A 54% decrease in the production of cellulose was found in rapeseed, and a 71% decrease in carrot. Hemicellulose production was also decreased in the flight samples compared to the ground controls. Ultrastructural analysis of the cell aggregates from the protoplasts cultured in orbit, demonstrated that hydrolysis and disappearance of reserve starch occurred in the flight cell plastids. The mitochondria were more varied in appearance in the flight samples than in the ground control cells. An increased frequency of the occurrence of folds formed by the plasmalemma together with an increase in the degree of complexity of these folds was also observed. Fluorescence analysis showed a decrease of the calcium content in cell cultures under space flight compared to the ground controls. One general effect of the stay onboard the space vehicle was a retardation of the regeneration processes. Callus cultures obtained from the flight samples grew very slowly compared to callus regenerated from the ground controls, and two years after the Biokosmos 9 flight there appears to be no further growth in the samples exposed to microgravity. Callus cultures from the ground controls, however, continue to grow well. A simulation experiment for IML-l performed in January 1990 at ESTEC (European Space Technology Center), The Netherlands, has resulted in regenerated plants. These observations are discussed and compared to the results obtained on Biokosmos 9.

Plant cell plasma membrane structure and properties under clinostatting.

Structural-functional organization of plasma membrane of pea roots seedling was investigated by methods of chemiluminescence, fluorescence probes, chromatography and freeze-fracture studies under normal conditions and clinostatting. Phase character of lipid peroxidation intensity was fixed. The initial phase of this process is characterized by lipid peroxidation decreasing with its next induction. The primary changes depending on free-radical mechanisms of lipid peroxidation were excellently revealed by chemiluminescence. Plasmalemma microviscosity increased on the average of 15-20% under microgravity at the initial stages of its phenomenon. There were major changes of phosphatidilcholine and phosphatidilethanolamine contents. The total quantity of phospholipids remained rather stable. Changes of phosphatide acid concentration point to degradation and phospholipids biosynthesis. There were increases of unsaturated fatty acids mainly at the expense of linoleic and linolenic acids and also a decrease of saturated fatty acid content at the expense of palmitic and stearic acids. Unsaturation index of fatty acids increased as well. On the whole fatty acid composition was variable in comparison with phospholipids. Probably it is one of mechanisms of maintaining of microviscosity within definite limits. Considerable structural changes in organization of plasmalemma protein-lipid complex were not revealed by the freeze-fracture studies.