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Open thoracoabdominal repair is the gold standard in the TAAA treatment. However, there are endovascular techniques, that sometimes may be an alternative, such as branched endovascular aneurysm repair (BEVAR) or implantation of the multilayer flow modulator (MFM). In this study, we aimed to assess differences in the aneurysm sac pressure (ASP) between patients undergoing BEVAR and MFM implantation. The study included 22 patients with TAAA (14 patients underwent BEVAR, while eight MFM implantation). The pressure sensor wire was placed inside the aneurysm. A measurement of ASP and aortic pressure (AP) was performed during the procedure. The systolic pressure index (SPI), diastolic pressure index (DPI), and pulse pressure index (PPI) were calculated as a quotient of the ASP and AP values. After the procedure, SPI and PPI were lower in the BEVAR group than in the MFM group. During a procedure, a drop in SPI and PPI was noted in patients undergoing BEVAR, while no changes were revealed in the MFM group. This indicates that BEVAR, but not MFM, is associated with a reduction in systolic and pulse pressure in the aneurysm sac in patients with TAAA.
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Aneurisma da Aorta Abdominal , Aneurisma da Aorta Torácica , Implante de Prótese Vascular , Procedimentos Endovasculares , Humanos , Aneurisma da Aorta Torácica/cirurgia , Procedimentos Endovasculares/métodos , Aneurisma da Aorta Abdominal/cirurgia , Prótese Vascular , Desenho de Prótese , Resultado do Tratamento , Fatores de Risco , Fatores de Tempo , Estudos RetrospectivosRESUMO
Purpose: Numerous cases of abdominal aortic aneurysm (AAA) enlargement, and even rupture, despite endovascular aneurysm repair (EVAR), have been documented. This has been linked to increased aneurysm sac pressure (ASP). We decided to conduct further research with the aim to identify correlations between ASP during EVAR and subsequent aneurysm enlargement. Patients and Methods: This experimental prospective study included 30 patients undergoing EVAR of infrarenal AAAs. Invasive ASP measurements were done using a thin pressure wire. Aortic pressure (AP) was measured using a catheter placed over the wire. Systolic pressure index (SPI), diastolic pressure index (DPI), mean pressure index (MPI), and pulse pressure index (PPI) were calculated both for ASP and AP. The results of follow-up computed tomography angiography (CTA) at 3 months were compared with baseline CTA findings. Results: During EVAR, a significant reduction was observed for SPI (from 98% to 61%), DPI (from 100% to 87%), MPI (from 99% to 74%), and PPI (from 97% to 34%). There were no significant correlations of pressure indices with an aneurysm diameter, cross-sectional area, velocity, thrombus shape and size, number of patent lumbar arteries, length and diameter of aneurysm neck, diameter of the inferior mesenteric artery, as well as diameter and angle of common iliac arteries. On the other hand, aneurysm neck angulation was significantly inversely correlated with reduced PPI. After combining CTA findings with pressure measurements, we identified a positive correlation between PPI and aneurysm enlargement (ratio of the cross-sectional area at the widest spot at baseline and at 3 months after EVAR). Conclusion: The study showed that ASP can be successfully measured during EVAR and can facilitate the assessment of treatment efficacy. In particular, PPI can serve as a prognostic factor of aneurysm enlargement and can help identify high-risk patients who remain prior monitoring.
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Aneurisma da Aorta Abdominal , Implante de Prótese Vascular , Procedimentos Endovasculares , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Aneurisma da Aorta Abdominal/cirurgia , Implante de Prótese Vascular/efeitos adversos , Procedimentos Endovasculares/efeitos adversos , Humanos , Prognóstico , Estudos ProspectivosRESUMO
The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFß1 and TGFß2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFß2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation.
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PURPOSE: The aim of this study was to determine the influence of factors such as temperature and glutaraldehyde (GA) concentration on the mechanical properties of porcine pericardia, in order to propose the recommended optimal conditions of a cross-linking process. It was also to verify whether tannic acid (TA), a natural cross-linking agent that stabilizes collagenous tissues by a different mechanism than GA, may positively influence the strength of pericardium. METHODS: The samples were incubated at various temperatures (4, 22, and 37°C) and GA concentration solutions (0.6, 1.5 and 3%) for 7 days. Three series were selected and additionally cross-linked with 0.3% TA for another 7 days. Mechanical properties of cross-linked pericardium samples, i.e., ultimate tensile strength (UTS) and elastic modulus (E) were measured in uniaxial tensile testing. The hyperelastic model for incompressible materials - isotropic by Ogden [24] and anisotropic by Fung [7] were utilized to describe the mechanical behaviour of treated pericardium. RESULTS: The temperature has an influence on cross-linking effects; the lowest values of UTS were reported for specimens cross-linked at 22 °C, while the mechanical properties of series treated at 4°C or 37°C were comparable. At a particular temperature of incubation, the GA concentrations have not affected the mechanical properties of tissues. The dependence between mechanical parameters and agent concentration was only observed for specimens treated with GA at 37 °C. CONCLUSIONS: The conditions of the cross-linking process affect the mechanical properties of the porcine pericardium. Room temperature (22 °C) and the concentration of 1.5% GA occurred to be ineffective. The mechanical properties of GA-treated pericardium were improved by an additional TA cross-linking.
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In this work, we aimed to determine the role of the mechanical, structural, and thermal properties of poly(l-lactide-co-glycolide-co-trimethylene carbonate) (P(l-LA:GA:TMC)) with shape memory in the formulation of implantable and biodegradable rods with aripiprazole (ARP). Hot melt extrusion (HME) and electron beam (EB) irradiation were applied in the formulation process of blank rods and rods with ARP. Rod degradation was carried out in a PBS solution. HPLC; NMR; DSC; compression and tensile tests; molecular weight (Mn); water uptake (WU); and weight loss (WL) analyses; and SEM were used in this study. HME and EB irradiation did not influence the structure of ARP. The mechanical tests indicated that the rods may be safely implanted using a pre-filled syringe. During degradation, no unfavorable changes in terpolymer content were observed. A decrease in the glass transition temperature and the Mn, and an increase in the WU and the WL were revealed. The loading of ARP and EB irradiation induced earlier pore formation and more intense WU and WL changes. ARP was released in a tri-phasic model with the lag phase; therefore, the proposed formulation may be administered as a delayed-release system. EB irradiation was found to accelerate ARP release.
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In this research, we synthesize and characterize poly(glycerol sebacate) pre-polymer (pPGS) (1H NMR, FTiR, GPC, and TGA). Nano-hydroxyapatite (HAp) is synthesized using the wet precipitation method. Next, the materials are used to prepare a PGS-based composite with a 25 wt.% addition of HAp. Microporous composites are formed by means of thermally induced phase separation (TIPS) followed by thermal cross-linking (TCL) and salt leaching (SL). The manufactured microporous materials (PGS and PGS/HAp) are then subjected to imaging by means of SEM and µCT for the porous structure characterization. DSC, TGA, and water contact angle measurements are used for further evaluation of the materials. To assess the cytocompatibility and biological potential of PGS-based composites, preosteoblasts and differentiated hFOB 1.19 osteoblasts are employed as in vitro models. Apart from the cytocompatibility, the scaffolds supported cell adhesion and were readily populated by the hFOB1.19 preosteoblasts. HAp-facilitated scaffolds displayed osteoconductive properties, supporting the terminal differentiation of osteoblasts as indicated by the production of alkaline phosphatase, osteocalcin and osteopontin. Notably, the PGS/HAp scaffolds induced the production of significant amounts of osteoclastogenic cytokines: IL-1ß, IL-6 and TNF-α, which induced scaffold remodeling and promoted the reconstruction of bone tissue. Initial biocompatibility tests showed no signs of adverse effects of PGS-based scaffolds toward adult BALB/c mice.
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Substitutos Ósseos/síntese química , Decanoatos/química , Durapatita/química , Glicerol/análogos & derivados , Polímeros/química , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Animais , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/química , Substitutos Ósseos/farmacologia , Substitutos Ósseos/uso terapêutico , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/fisiologia , Células Cultivadas , Feminino , Glicerol/química , Humanos , Invenções , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Osteoblastos/efeitos dos fármacos , Osteoblastos/fisiologia , Osteogênese/efeitos dos fármacos , Polímeros/síntese química , Porosidade , Engenharia Tecidual/tendênciasRESUMO
BACKGROUND: An endoleak is a typical complication of endovascular aneurysm repair (EVAR). It is characterized by persistent blood flow between a stent graft and the aneurysm sac. Usually, it can be visualized during primary EVAR, but in many cases, this remains impossible. Therefore, other methods of endoleak assessment are urgently needed. The measurement of aneurysm sac pressure (ASP) seems to be a promising direction of research in this area. OBJECTIVES: We aimed to evaluate the safety and efficacy of a new method for invasive pressure measurement inside the abdominal aortic aneurysm (AAA) during EVAR. We also assessed a correlation between pressure values and early angiographic occurrence of an endoleak after the procedure. MATERIAL AND METHODS: A total of 20 patients with AAA were included in this experimental prospective study. During EVAR, systolic, diastolic and mean pressure values were recorded both for ASP and aortic pressure (AP) before procedure, after stent graft opening and after final stent graft ballooning. RESULTS: The measurements were successfully obtained in all participants without any complications. There were no significant differences between all ASP and AP before procedure. After the procedure, blood pressure significantly decreased in the aneurysm sac but not in the aorta. Systolic ASP was significantly lower than systolic AP both after stent graft opening (80.4 ±20.9 mm Hg compared to 110.7 ±21.6 mm Hg, p < 0.01) and after its balloon post-dilatation (65.6 ±26.1 mm Hg compared to 107.4 ±22.1 mm Hg, p < 0.001). Diastolic ASP decreased significantly in comparison to diastolic AP only after stent graft ballooning (48.0 ±14.6 mm Hg compared to 56.4 ±13.6 mm Hg, p < 0.05). CONCLUSIONS: Our study confirmed that the novel method for the measurement of ASP during EVAR, using a thin pressure wire, is feasible and safe.
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Aneurisma da Aorta Abdominal , Implante de Prótese Vascular , Procedimentos Endovasculares , Aneurisma da Aorta Abdominal/diagnóstico por imagem , Aneurisma da Aorta Abdominal/cirurgia , Implante de Prótese Vascular/efeitos adversos , Endoleak/diagnóstico por imagem , Endoleak/etiologia , Procedimentos Endovasculares/efeitos adversos , Humanos , Estudos Prospectivos , Stents , Resultado do TratamentoRESUMO
INTRODUCTION: Extracellular matrix (ECM) proteins have been associated with atherosclerotic complications, such as plaque rupture, calcification and aneurysm. It is not clear what role different types of collagen play in the pathomechanism of atherosclerosis. The aim of the study was to analyze the content of elastin and major types of collagen in the aortic wall and how they associated are with course of atherosclerosis. MATERIAL AND METHODS: In this work we present six biochemical parameters related to ECM proteins and collagen-specific amino acids (collagen type I, III, and IV, elastin, proline and hydroxyproline) analyzed in 106 patients' aortic wall specimens characterized by different degree of atherosclerosis. Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (LC/ESI-MS/MS), ELISA and immunohistochemical methods were used. The severity of atherosclerosis was assessed on the six-point scale of the American Heart Association, taking into account the number and location of foam cells, the presence of a fatty core, calcium deposits and other characteristic atherosclerotic features. RESULTS: The results show that there is a relationship between the content of collagen-specific amino acids and development of atherosclerosis. The degree of atherosclerotic lesions was negatively correlated with the content of proline, hydroxyproline and the ratio of these two amino acids. Calcium deposits and surrounding tissue were compared and it was demonstrated that the ratio of type I collagen to type III collagen was higher in the aortic tissue than in aortic calcification areas, while the ratio of collagen type III to elastin was smaller in the artery than in the calcium deposits. CONCLUSIONS: We suggest that increase in collagen type III presence in the calcification matrix may stem from disorders in the structure of the type I and III collagen fibers. These anomalous fibers are likely to favor accumulation of the calcium salts, an important feature of the process of atheromatosis.
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Aorta/metabolismo , Aterosclerose/metabolismo , Colágeno/metabolismo , Elastina/metabolismo , Calcificação Vascular/metabolismo , Adulto , Idoso , Colágeno/análise , Elastina/análise , Feminino , Humanos , Hidroxiprolina/metabolismo , Masculino , Pessoa de Meia-Idade , Prolina/metabolismoRESUMO
Novel biocomposites of poly(L-lactide) (PLLA) and poly(l-lactide-co-glycolide) (PLLGA) with 10 wt.% of surface-modified hydroxyapatite particles, designed for applications in bone tissue engineering, are presented in this paper. The surface of hydroxyapatite (HAP) was modified with polyethylene glycol by using l-lysine as a linker molecule. The modification strategy fulfilled two important goals: improvement of the adhesion between the HAP surface and PLLA and PLLGA matrices, and enhancement of the osteological bioactivity of the composites. The surface modifications of HAP were confirmed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), TGA, and elemental composition analysis. The influence of hydroxyapatite surface functionalization on the thermal and in vitro biological properties of PLLA- and PLLGA-based composites was investigated. Due to HAP modification with polyethylene glycol, the glass transition temperature of PLLA was reduced by about 24.5 °C, and melt and cold crystallization abilities were significantly improved. These achievements were scored based on respective shifting of onset of melt and cold crystallization temperatures and 1.6 times higher melt crystallization enthalpy compared with neat PLLA. The results showed that the surface-modified HAP particles were multifunctional and can act as nucleating agents, plasticizers, and bioactive moieties. Moreover, due to the presented surface modification of HAP, the crystallinity degree of PLLA and PLLGA and the polymorphic form of PLLA, the most important factors affecting mechanical properties and degradation behaviors, can be controlled.
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Materiais Biocompatíveis/química , Durapatita/química , Poliésteres/química , Cristalização/métodos , Teste de Materiais , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Propriedades de Superfície , TemperaturaRESUMO
Laser-based technologies are extensively used for polymer surface patterning and/or texturing. Different micro- and nanostructures can be obtained thanks to a wide range of laser types and beam parameters. Cell behavior on various types of materials is an extensively investigated phenomenon in biomedical applications. Polymer topography such as height, diameter, and spacing of the patterning will cause different cell responses, which can also vary depending on the utilized cell types. Structurization can highly improve the biological performance of the material without any need for chemical modification. The aim of the study was to evaluate the effect of CO2 laser irradiation of poly(L-lactide) (PLLA) thin films on the surface microhardness, roughness, wettability, and cytocompatibility. The conducted testing showed that CO2 laser texturing of PLLA provides the ability to adjust the structural and physical properties of the PLLA surface to the requirements of the cells despite significant changes in the mechanical properties of the laser-treated surface polymer.
RESUMO
PURPOSE: Irradiations by CO2 laser poly(lactic-co-glycolic acid) surface lead to alterations of physicochemical properties of a copolymer. Effects of PLGA irradiations are results of photochemical and photothermal processes leading to polymer degradation. The scale of the degradation depends on the inducted surface modification. Hence the main goal of presented studies was to define the influence of CO2 laser irradiation with different process parameters, inducing three cases of surface modification, on mechanical properties and topography of PLGA during degradation in the aqueous environment. METHODS: Hydrolytic degradation were performed in distilled (demineralized) water. Mechanical properties were conducted in accordance with the PN-EN ISO 527-3:1998 standard. pH of incubating solution, specimens' topography, mass and geometrical dimensions were controlled during process. RESULTS: During the hydrolytic degradation, gradual changes in failure mode were observed from ductile failure characteristic for untreated PLGA to brittle failure of incubated PLGA regardless of the case of triggered modification. Tensile strength decreased with degradation time regardless of the case of surface modification with insignificant fluctuation in means Young's moduli. pH for each case decreased and topography od specimens become smoother with incubation time. CONCLUSIONS: PLGA surface modification by CO2 laser below the ablation threshold (P1) and at the ablation threshold (P2) leaded to surface functionalization, however, irradiation above the ablation threshold (P3) caused marked degradation of PLGA and accelerated specimens disintegration during incubation in the aquatic environment.
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Lasers de Gás , Fenômenos Mecânicos , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Próteses e Implantes , Módulo de Elasticidade , Concentração de Íons de Hidrogênio , Hidrólise , Estresse Mecânico , Propriedades de Superfície , Resistência à Tração , Fatores de TempoRESUMO
Atherosclerotic plaques are characterized by structural heterogeneity affecting aortic behaviour under mechanical loading. There is evidence of direct connections between the structural plaque arrangement and the risk of plaque rupture. As a consequence of aortic plaque rupture, plaque components are transferred by the bloodstream to smaller vessels, resulting in acute cardiovascular events with a poor prognosis, such as heart attacks or strokes. Hence, evaluation of the composition, structure, and biochemical profile of atherosclerotic plaques seems to be of great importance to assess the properties of a mechanically induced failure, indicating the strength and rupture vulnerability of plaque. The main goal of the research was to determine experimentally under uniaxial loading the mechanical properties of different types of the human abdominal aorta and human aortic atherosclerotic plaques identified based on vibrational spectra (ATR-FTIR and FT-Raman spectroscopy) analysis and validated by histological staining. The potential of spectroscopic techniques as a useful histopathological tool was demonstrated. Three types of atherosclerotic plaques - predominantly calcified (APC), lipid (APL), and fibrotic (APF) - were distinguished and confirmed by histopathological examinations. Compared to the normal aorta, fibrotic plaques were stiffer (median of EH for circumferential and axial directions, respectively: 8.15 MPa and 6.56 MPa) and stronger (median of σM for APLc = 1.57 MPa and APLa = 1.64 MPa), lipidic plaques were the weakest (median of σM for APLc = 0.76 MPa and APLa = 0.51 MPa), and calcified plaques were the stiffest (median of EH for circumferential and axial directions, respectively: 13.23 MPa and 6.67 MPa). Therefore, plaques detected as predominantly lipid and calcified are most prone to rupture; however, the failure process reflected by the simplification of the stress-stretch characteristics seems to vary depending on the plaque composition.
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Placa Aterosclerótica , Aorta , Humanos , RupturaRESUMO
PURPOSE: The main purpose of this study was a detailed analysis of the mechanical and structural characteristics of human abdominal aneurysms in comparison with normal abdominal aortae and determination of the correlations between their mechanical behaviour and the microstructural content. METHODS: Various mechanical properties, i.e., mechanical failure properties, elastic moduli, inflection point coordinates, index of anisotropy and incompressibility were determined under uniaxial loading conditions in the circumferential and axial directions. Constitutive parameters were derived from the commonly used constitutive model proposed by Holzapfel et al. [9]. The microstructural arrangement was examined by histological staining supported by scanning electron microscopy analysis. The content of collagen fibres and elastic lamellae was tested in relation to mechanical properties and constitutive parameters. RESULTS: Significant differences were found in the microstructural arrangement and layer composition of the aneurysmal specimens, compared to the normal aorta group. The mechanical properties and constitutive parameters of the aneurysmal specimens were significantly altered, indicating a weakening of the load-bearing properties of the walls of the aneurysms. A comparative analysis discovered significant correlations between structural composition and mechanical parameters, in particular with respect to the number of collagen fibres and failure stress, which can be important for clinical evaluation of abdominal aortic aneurysm (AAA) rupture. CONCLUSIONS: Changes in the content of collagen fibres and elastic lamellae correlate with mechanical and constitutive parameters, indicating AAA severity.
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Aneurisma da Aorta Abdominal/patologia , Aneurisma da Aorta Abdominal/fisiopatologia , Colágeno/metabolismo , Elasticidade , Idoso , Anisotropia , Fenômenos Biomecânicos , Colágeno/ultraestrutura , Feminino , Humanos , Masculino , Estresse Mecânico , Túnica Média/ultraestruturaRESUMO
The aim of this work is to determine the impact of development of atherosclerosis on dissection of the human thoracic aorta on the basis of an analysis of the mechanical properties of the interfaces between its layers. The research material consisted of 17 pathologically unchanged aortae and 74 blood vessels with atherosclerotic lesions, which were classified according to the histological classification by Stary. The subject of the analysis were the interfaces between the adventitia and the media-intima complex (A-MIC) and between the intima and the media-adventitia complex (I-MAC). The mechanical properties of the above interfaces were determined by the peeling test in the longitudinal and circumferential directions. The results indicate that development of atherosclerosis reduces vessel wall resistance to delamination. The greatest risk of dissection occurs at stage IV of the disease. In this case, energy values are lower by about 28% for the I-MAC interface and by 39% for the A-MIC interface compared with normal tissues. Lower values of mean force and energy were obtained for the I-MAC interface, indicating that this interface is more susceptible to delamination. The mechanical properties of the A-MIC interfaces are directional.
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Aorta Torácica/fisiopatologia , Aterosclerose/fisiopatologia , Túnica Íntima/fisiopatologia , Resistência Vascular/fisiologia , Fenômenos Biomecânicos/fisiologia , Humanos , Estresse MecânicoRESUMO
OBJECTIVE: The signature processes during atherosclerosis development are arterial calcification and accumulation in the arterial walls of proteins that are specific to bone and cartilage, e.g., collagen type II. The purpose of this study was to characterize localization of collagen type II and quantify its content in human arteries. RESULTS: The study was conducted on sections of thoracic and abdominal aortas (n=97) subjected to histological evaluation and classified into six grades according to the Stary scale of the atherosclerosis severity. Three types of samples were distinguished from the group of arteries: (1) without macroscopically visible calcifications, (2) with macroscopically visible calcifications dispersed within the arterial wall, and (3) calcium deposits isolated from the walls tested with respect to the segment of the artery from which they had originated. The results demonstrate that both cholesterol and collagen type II content are significantly higher in samples with calcification, whereas collagen type II is localized mainly in the tissue around the calcium deposit. A positive correlation has been shown between the levels of collagen type II and cholesterol (r=0.57, P<.05). A similar trend was observed with respect to the grade of atherosclerosis (r=0.43, P<.05). CONCLUSIONS: The amount of collagen type II is higher in the tissue around the calcium deposit. The correlation was observed between the quantityof collagen type II, the grade of atherosclerosis, and cholesterol.
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Aorta Abdominal/química , Aorta Torácica/química , Doenças da Aorta/metabolismo , Aterosclerose/metabolismo , Cálcio/análise , Colágeno Tipo II/análise , Calcificação Vascular/metabolismo , Adulto , Idoso , Aorta Abdominal/patologia , Aorta Torácica/patologia , Doenças da Aorta/patologia , Aterosclerose/patologia , Biomarcadores/análise , Colesterol/análise , Feminino , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Placa Aterosclerótica , Índice de Gravidade de Doença , Calcificação Vascular/patologiaRESUMO
The biophysical properties of the annulus fibrosus of the intervertebral disc are determined by collagen and elastin fibres. The progression of scoliosis is accompanied by a number of pathological changes concerning these structural proteins. This is a major cause of dysfunction of the intervertebral disc. The object of the study were annulus fibrosus samples excised from intervertebral discs of healthy subjects and patients treated surgically for scoliosis in the thoracolumbar or lumbar spine. The research material was subjected to structural analysis by light microscopy and quantitative analysis of the content of collagen types I, II, III and IV as well as elastin by immunoenzymatic test (ELISA). A statistical analysis was conducted to assess the impact of the sampling site (Mann-Whitney test, α=0.05) and scoliosis (Wilcoxon matched pairs test, α=0.05) on the obtained results. The microscopic studies conducted on scoliotic annulus fibrosus showed a significant architectural distortion of collagen and elastin fibres. Quantitative biochemical assays demonstrated region-dependent distribution of only collagen types I and II in the case of healthy intervertebral discs whereas in the case of scoliotic discs region-dependent distribution concerned all examined proteins of the extracellular matrix. Comparison of scoliotic and healthy annulus fibrosus revealed a significant decrease in the content of collagen type I and elastin as well as a slight increase in the proportion of collagen types III and IV. The content of collagen type II did not differ significantly between both groups. The observed anomalies are a manifestation of degenerative changes affecting annulus fibrosus of the intervertebral disc in patients suffering from scoliosis.
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Anel Fibroso/patologia , Anel Fibroso/fisiologia , Colágeno/química , Elastina/química , Disco Intervertebral/patologia , Disco Intervertebral/fisiologia , Humanos , Escoliose/patologiaRESUMO
PURPOSE: There are two families of fibres taking part in the process of mechanical loads transfer, i.e. elastin and collagen fibres. Their number, spatial arrangement and specific properties determine the capacity of a blood vessels to resist mechanical loads resulting from the impact of blood on vessel walls. The purpose of the present paper is to define the load-bearing capacities of elastin and collagen scaffolds equivalent to natural fibre arrangements of human aorta and produced by selective digestion. METHODS: Samples of thoracic human aortas were digested by using phosphate buffer of trypsin at pH 8.0 for 22 hours in order to degrade elastin and by autoclaving followed by incubation in 90% formic acid for 22 hours. The efficacy of digestion was assessed immunohistochemically. Mechanical properties of pre-stretched native and digested samples were determined by uniaxial tensile test. RESULTS: Samples subjected to autoclaving have been successfully deprived of both types of collagen and elastin has been intact. Treatment with trypsin caused a removal of elastin and the presence of type I and IV collagen was demonstrated. Digestion of aortic samples either by formic acid or trypsin has resulted significantly decreasing mechanical properties in comparison with native samples. CONCLUSIONS: Collagen and elastin scaffold-like stuctures have been effectively produced by selective digestion of thoracic human aorta and their contribution to the load-bearing process was evaluated. Isolated collagen network are more durable and stiffer and less deformable than elastin network, hence are responsible for load-bearing process at higher strain since the range of working of elastin is at lower strain values.
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Aorta Torácica/fisiologia , Colágeno/fisiologia , Elastina/fisiologia , Mecanotransdução Celular/fisiologia , Modelos Cardiovasculares , Suporte de Carga/fisiologia , Adulto , Força Compressiva/fisiologia , Simulação por Computador , Módulo de Elasticidade/fisiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estresse Mecânico , Resistência à Tração/fisiologiaRESUMO
Among the currently used methods of monitoring human tissues and their components many types of research are distinguished. These include spectroscopic techniques. The advantage of these techniques is the small amount of sample required, the rapid process of recording the spectra, and most importantly in the case of biological samples - preparation of tissues is not required. In this work, vibrational spectroscopy: ATR-FTIR and Raman spectroscopy will be used. Studies are carried out on tissues: tendons, blood vessels, skin, red blood cells and biological components: amino acids, proteins, DNA, plasma, and deposits.
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Especificidade de Órgãos , Análise Espectral Raman , Animais , Tecnologia Biomédica , DNA/análise , Humanos , Concentração de Íons de Hidrogênio , Masculino , Proteínas/análise , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Glycation consists in formation of advanced glycation end-products (AGE) during non-enzymatic reaction between reducing sugars and proteins, lipids or nucleic acids. This review is focused mainly on glycation of collagen and its role in acceleration of vascular disease. Collagen is an extracellular matrix protein characterized by unique structure forming fibrils with great anti-tensile and anti-breaking strength. The protein builds the connective tissue and is responsible for biomechanical properties of blood vessels. It is reported that higher content of glycated collagen correlates with lower elasticity and greater toughness of the vessel walls and, as a consequence, a faster rate of atherosclerosis development. Numerous mechanisms connected with AGE formation are involved in atherogenesis, among others: receptor-mediated production of free radicals, triggering an inflammatory process, activation of leukocytes and thrombocytes, facilitation of LDL binding, change in level of growth factors, adhesion molecules, MMP and some other proteins' expression. The coverages allow the development of therapeutic strategies to prevent or slow down the pathological processes connected with glycation of collagen and other proteins in the artery wall. The main strategies are based on limitation of exogenous AGE, consumption of products which contain rutin, treatment with drugs which inhibit AGE formation, such as pyridoxamine, and chemicals which are able to cleave already formed AGE protein-protein crosslinks, such as ALT-711.
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Artérias/fisiopatologia , Aterosclerose/metabolismo , Colágeno/metabolismo , Proteínas da Matriz Extracelular/biossíntese , Produtos Finais de Glicação Avançada/metabolismo , Fenômenos Biomecânicos , Tecido Conjuntivo/metabolismo , Elasticidade , Glicosilação , Humanos , Piridoxamina/metabolismo , Doenças Vasculares/metabolismoRESUMO
Among the currently used methods of monitoring human tissues and their components many types of research are distinguished. These include spectroscopic techniques. The advantage of these techniques is the small amount of sample required the rapid process of recording the spectra, and most importantly in the case of biological samples - preparation of tissues is not required. In this work vibrational spectroscopy: ATR-FTIR and Raman spectroscopy will be used. Studies are carried out on tissues: tendons, blood vessels, skin, red blood cells and biological components: amino acids, proteins, DNA, plasma, and deposits.