Organ distribution and early pathogenesis of Streptococcus equi subsp. zooepidemicus in swine.

Streptococcus equi subsp. zooepidemicus is an emerging pathogen of pigs, resulting in high-mortality outbreaks of septicaemia and abortions. Here, we investigated the early pathogenesis of S. zooepidemicus in pigs following oronasal inoculation. Fourteen pigs were inoculated with live cultures of S. zooepidemicus ST-194, and monitored at 2,4, 8, and 24 h post-inoculation. Necropsies were performed to assess gross lesions and collect samples for bacterial culture and PCR analysis at each time point. Our findings revealed that S. zooepidemicus was detectable in various organs as early as 2 h post-inoculation, including liver and spleen, demonstrating rapid dissemination within the host. Tonsil samples consistently harboured live S. zooepidemicus throughout the study period, suggesting their potential for epidemiological sampling and diagnostics. Moreover, the presence of varying bacterial loads in mesenteric lymph nodes indicated persistence, replication, and a potential source for shedding. Further studies are required to determine the initial site of replication.

Survival of Streptococcus equi subsp. zooepidemicus on environmental samples is affected by material type and incubation temperature.

Streptococcus equi subsp. zooepidemicus is an opportunistic pathogen associated with disease in a range of domestic and wild animals. Despite its importance, very limited data is available on its survival and persistence on the environment. The goal of this study was to evaluate survival of S. zooepidemicus under ideal culture conditions and farm-like setting, in various surface types. Rubber, plastic, wood, and concrete samples were sterilized and inoculated with 109 CFU/mL of S. zooepidemicus with or without feces, and cultured under ideal conditions (37 °C, 5% CO2) or farm-like settings (20oC on air) for a maximum period of 25 days (n = 3/material/environment/feces-group/time-point). Under ideal conditions without feces, the bacterium survived for up to 17 days on plastic and rubber surfaces, 4 days on wood and less than 1 day on concrete (P < 0.05 between materials). Samples under ideal conditions with feces and farm-like settings without feces were negative by day 1 post-inoculation, regardless of the surface material used. Wood and concrete allowed S. zooepidemicus persistence for up to 3 days under farm-like settings when feces were present. This data suggests that environmental persistence of S. zooepidemicus is affected by surface type and incubation temperature.

COVID-19 in Farm Animals: Host Susceptibility and Prevention Strategies.

COVID-19 is caused by the virus SARS-CoV-2 that belongings to the family of Coronaviridae, which has affected multiple species and demonstrated zoonotic potential. The COVID-19 infections have been reported on farm animals (e.g., minks) and pets, which were discussed and summarized in this study. Although the damage of COVID-19 has not been reported as serious as highly pathogenic avian influenza (HPAI) for poultry and African Swine Fever (ASF) for pigs on commercial farms so far, the transmission mechanism of COVID-19 among group animals/farms and its long-term impacts are still not clear. Prior to the marketing of efficient vaccines for livestock and animals, on-farm biosecurity measures (e.g., conventional disinfection strategies and innovated technologies) need to be considered or innovated in preventing the direct contact spread or the airborne transmission of COVID-19.