RESUMO
Eucalyptus oil has been used since ancient times for its bactericidal, anti-inflammatory, analgesic and sedative effects. In recent years, the action of Eucalyptus oil has been scientifically proven, and there have been reports that Eucalyptus oil suppresses the production of chemokines, cytokines and lipid mediators in basophils, alveolar macrophages and monocytes. Based on this information, we aimed to verify whether Eucalyptus oil can be used for allergic dermatitis, the incidence of which has been increasing among human skin diseases. This effect was verified using a mouse IgE-mediated local allergic model. In conclusion, topical application of Eucalyptus oil suppressed oedema and vascular permeability enhancement due to IgE-mediated allergic on the skin. In addition, we also verified the degranuration of mast cells, which is a part of its action, and examined whether 1,8-cineole, which is the main component of Eucalyptus oil, suppresses the phosphorylation of PLCγ and p38 directly or indirectly. 1,8-cineole was found to suppress degranulation of mast cells.
Assuntos
Degranulação Celular , Regulação para Baixo , Óleo de Eucalipto/uso terapêutico , Hipersensibilidade/tratamento farmacológico , Imunoglobulina E/metabolismo , Mastócitos/fisiologia , Receptores de IgE/metabolismo , Transdução de Sinais , Animais , Células da Medula Óssea/efeitos dos fármacos , Cálcio/metabolismo , Degranulação Celular/efeitos dos fármacos , Quimiocinas/metabolismo , Modelos Animais de Doenças , Regulação para Baixo/efeitos dos fármacos , Eucaliptol/farmacologia , Inflamação/patologia , Mediadores da Inflamação/metabolismo , Espaço Intracelular/metabolismo , Mastócitos/efeitos dos fármacos , Camundongos , Modelos Biológicos , Anafilaxia Cutânea Passiva/efeitos dos fármacos , Fosfolipase C gama/metabolismo , Fosforilação , Transdução de Sinais/efeitos dos fármacos , Quinase Syk/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Quinases da Família srcRESUMO
In this study, we investigated how tannic acid (TA) protects the skin from inflammation caused by external irritation. The effects of TA were evaluated using a mouse 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced skin inflammation model and a reconstructed human epidermal model. We then used Lucifer Yellow for visual confirmation of TA's suppression effect at the stratum corneum (SC) surface. TA treatment of the skin prevented Lucifer Yellow from permeating the skin. This result suggests that TA acts as a barrier against external stimulants such as TPA and artificial sweat on the SC surface.
Assuntos
Epiderme/efeitos dos fármacos , Pele/patologia , Taninos/farmacologia , Animais , Dermatite de Contato , Modelos Animais de Doenças , Corantes Fluorescentes/química , Inflamação/induzido quimicamente , Isoquinolinas , Masculino , Camundongos , Camundongos Endogâmicos ICR , Permeabilidade , Pele/efeitos dos fármacos , Dermatopatias/induzido quimicamente , Suor , Acetato de TetradecanoilforbolRESUMO
We examined motility, plasma membrane integrity, and binding capacity to homologous zona pellucidae (ZP) of frozen/thawed epididymal cat sperm as a model species for endangered felines. Epididymal spermatozoa from 20 domestic cats were frozen with freezing egg-yolk extender containing 3.0% glycerol in 0.25-ml straws. Post-thaw motility and plasma membrane integrity of the frozen/thawed spermatozoa were 31.8 +/- 2.4% and 32.2 +/- 4.2%, respectively. The frozen/thawed spermatozoa were co-cultured with frozen/thawed immature homologous oocytes with intact ZP for 3 h to examine their ability to bind to the ZP. Sixteen of the 20 frozen/thawed sperm samples demonstrated the ability to bind to ZP. These results indicated that the freezing system for epididymal sperm used in the present study gives appropriate information for banking the genetic resources of wild felid species.