[A study of ligand binding to protein using resonance energy transfer from the isoindole fluorescent label to ligand]. / Issledovanie sviazyvaniia ligandov s belkom s pomoshch'iu perenosa énergii s izoindol'noi fluorestsentnoi metki na ligand.

A method for studing the binging of ligands absorbing the light in the region of 350-550 nm to protein is described. The method is based on resonance energy transfer between the fluorescent label covalently bound to the protein and the ligand. The isoindole label, a product of the reaction of the protein with o-phthalaldehyde in the presence of 2-mercaptoethanol, was used as a fluorescent donor. The method was used to determine the binding parameters of a fluorescent probe (a naphthalimide derivative) with human serum albumin. A comparison of the results obtained by the resonance energy and transfer by equilibrium dialysis showed a high accuracy of the resonance energy transfer method.

[Changes in macro- and micro element composition in the organs of irradiated animals]. / Izmenenie makro- i mikroélementnogo sostava v organakh obluchennykh zhivotnykh.

We studied the content of Al, Cd, Co, Cu, Fe, Mg, Mn, P, Pb, S, and Zn in the liver, kidneys, lungs, and spleen of mice with total radiation dose of 7.5 Gy using atomic emission spectral analysis with an inductively coupled argon plasma. The qualitative content of macro- and microelements and coordination between their concentrations statistically determined from coefficients of linear correlation differ between the tissues of the irradiated and control animals. Radiation damage decreases phosphorus content in all studied organisms and is a marker of disturbed conjugated oxidative phosphorylation. The most significant radiation-induced disturbance of macro- and microelement balance was detected in the spleen; it features decreased content of phosphorus, magnesium, and cobalt, as well as increased content of zinc and aluminum and considerably increased iron content. The revealed macro- and microelements disbalance in spleen, liver, kidneys, and lungs can be considered as a test for primary biological response to radiation damage.

[Size of a human serum albumin molecule in solution]. / Razmer molekuly syvorotochnogo al'bumina cheloveka v rastvore.

The size of a human serum albumin molecule in aqueous solution containing 150 mM NaCl was studied using small-angle neutron scattering. The molecular radius of gyration was estimated to be 27.4 +/- 0.35 A. The compact sphere should have a smaller radius of gyration, whereas the popular human serum albumin model, a "cigar" 136 A long, should correspond to a greater radius of gyration. Possible shapes of the human serum albumin molecule which are in accordance with the results obtained, are the following: an extended ellipsoid less than 110 A of length or a nonsymmetrical oblate ellipsoid with a diameter of 85 A. The oblate ellipsoid might be close to the heart"-shaped structure of the crystalline human serum albumin molecule. The size of the albumin molecule does not change significantly as pH increases to 8.9. The possibility of the dynamic coexistence of various human serum albumin conformers in solution is discussed.

[Method for assessing involution ophthalmoendotoxicosis by fluorescent analysis of lacrimal fluid]. / Sposob otsenki involiutsionnogo oftal'moéndotoksikoza putem fliuorestsentnogo issledovaniia sleznoi zhidkosti.

A method for evaluating the binding capacity of aqueous humor albumin as endoophthalmotoxicosis indicator is proposed, consisting in evaluation of this parameter in the lacrimal fluid by fluorescent probe K-35. A correlation between the studied parameter in aqueous humor, lacrimal fluid, and blood serum has been detected. Albumin binding capacity in the above biological fluids of patients with involution ophthalmopathy was low.

[The characteristics of the slow electrical activity of the brain in those who worked in the cleanup of the aftermath of the accident at the Chernobyl Atomic Electric Power Station]. / Osobennosti medlennoi élektricheskoi aktivnosti golovnogo mozga u likvidatorov posledstvii avarii na Chernobyl'skoi AES.

We studied the slow electrical activity of the brain in people subjected to irradiation as a result of the Chernobyl accident and in healthy donors. The mean constant potential, which is an index of energy metabolism in different regions of the cerebral cortex, is reliably lower in eliminators, as compared to the donors, thus suggesting a postradiation energy deficiency of the brain. The structure of correlations between the constant potential indices is different: the number of significant correlations in the group of eliminators is lower by 30% than in the group of donors, thus suggesting disturbed intercentral relations in the CNS under the conditions of postradiation energy deficiency of the brain.

[Physical criteria of pathological processes. I. Change in the functional hemispheric asymmetry at various stages of rheumatic diseases]. / Fizicheskie kriterii patologicheskikh protsessov. I.Izmenenie funktsional'noi mezhpolusharnoi asimmetrii na raznykh stadiiakh revmaticheskikh zabolevanii.

The functional interhemispheric asymmetry of brain in patients with systemic rheumatic diseases was estimated by measuring brain direct current potentials. It is shown that different types of functional interhemispheric asymmetry correspond to different stages of systemic rheumatic diseases. At early stages, the left hemisphere asymmetry dominates, at expressed stages, the right hemisphere asymmetry is prevalent, and the terminal stage is characterized by the loss of functional interhemispheric asymmetry.

[Activity of the autoimmune process during systemic rheumatic diseases and distribution of constant brain potential]. / Aktivnost' autoimmunnogo protsessa pri sistemnykh revmaticheskikh zabolevaniiakh i raspredelenie postoiannogo potentsiala golovnogo mozga.

The data obtained upon examination of patients with systemic rheumatic diseases were analyzed by the methods of multiple regression and step-by-step discriminant analysis. Is was shown that the activity of autoimmune process correlates with the parameters of distribution of constant potential level in the brain of each patient, irrespective of diagnosis. The scatter of potential values was the most important predictor of activity. An increase in the activity of the autoimmune process correlated with a decrease in the scatter of potential values at recording points; the cerebral cortex became equipotential.

[Constant brain potentials as neurophysiological markers of autoimmune process]. / Postoiannye potentsialy golovnogo mozga kak neirofiziologicheskie markery autoimmunnogo protsessa.

Neuroimmune interactions in systemic rheumatic diseases were studied. The state of the central nervous system was assessed from the parameters of constant brain potentials, and the state of the immune system, from a complex of immunobiochemical parameters. The highest multiple correlation coefficients were revealed between the immunobiochemical parameters and the parameters of the constant brain potential, which characterize linear and standard deviations of potentials in temporal zones from potentials at other points of recording. The results are discussed in terms of structural and functional integration of the immune and nervous systems.

[Physical criteria of pathological processes. III. Structure of the correlation between the level of steady brain potential with serum complement proteins in rheumatic diseases]. / Fizicheskie kriterii patologicheskikh protsessov. III. Struktura korreliatsionnoi urovnia postoiannogo potentsiala golovnogo mozga s belkami syvorotochnogo komplementa pri revmaticheskikh zabolevaniiakh.

The activity of serum complement proteins and the level of steady brain potential in patients with systemic lupus erythematosus and rheumatoid arthritis were determined. The statistical analysis indicated that there is a correlation between the parameters of the complement system and neurophysiological characteristics. The differences between rheumatoid arthritis and systemic lupus erythematosus are observed in the mean values of activity of complement proteins and the steady potential, their dispersions, and the structure of correlations between immunological and neurophysiological parameters.

[Physical criteria of pathological processes. II. Modulating role of functional interhemisphere asymmetry in forming and immune response in rheumatic diseases]. / Fizicheskie kriterii patologicheskikh protsessov. II. Moduliruiushchaia rol' funktsional'noi mezhpolusharnoi asimmetrii v formirovanii immunnogo otveta pri revmaticheskikh zabolevaniiakh.

The level of the brain permanent potential as a physiological criterion of the functional interhemisphere asymmetry, as well as immunological and biochemical characteristics of peripheral blood in patients with systemic rheumatic diseases were studied. It was found that the distribution of the characteristics of immune response in patients with different types of interhemisphere asymmetry. Significant differences were observed for average values of biochemical and immunological characteristics, their dispersions, as well as for the structure of relationships between the type of the interhemisphere asymmetry and immunobiochemical characteristics.