RESUMO
The research of hemocytes of laboratory cultivated pond snails Lymnaea stagnalis originating from two areas near Chernobyl with different radiation load has been carried out by means of comet assay. Significant interpopulation distinctions in parameters of DNA-comets have been revealed by means of the software analysis of hemocyte DNA-comet images: hemocytes of mollusks from radiationally unfavourable "Perstok" population have contained a twice DNA quantity in comparison with "Pripyat" population, and also have been statistically more resistant to the influence of strontium. Strontium reduces the amount of DNA in hemocytes, at that strontium dose of 0.5 MPC is already substantial (i.e., causes a negative impact).for snail Lymnaea stagnalis. According to a RAPD-analysis previously conducted, the snails of two populations are characterized by high genetic similarity, on the basis of which a hypothesis is suggested that the observed differences in the DNA content of hemocytes and resistance to strontium are inherited as prolonged adaptive modification (epigenetic change) in response to the intensive damaging impact of environment. Comet assay can be its indicator while carrying out the environmental monitoring.
Assuntos
Acidente Nuclear de Chernobyl , DNA/genética , DNA/metabolismo , Raios gama/efeitos adversos , Hemócitos/metabolismo , Lymnaea/genética , Lymnaea/metabolismo , Animais , Estrôncio/efeitos adversosRESUMO
The research of hemocytes of snail Lymnaea stagnalis from regions with different environmental load has been carried out by means of DNA-comet assay. Significant interpopulation distinctions in parameters of hemocytes DNA comets, and also significant differences of sensitivity of hemocyte genetic matherial in snails form different ecological zones to the influence of external damaging factors (in particular, heavy metals) have been revealed by means of the software analysis of hemocyte DNA-comet images. Since the two populations of mollusks are characterized by high genetic identity, the different levels of proliferative processes in hemocytes of snail Lymnaea stagnalis from different ecological zones (that we revealed using the comet assay) may act as an indicator of the intensity of damaging effects and environmental quality.
Assuntos
Radioisótopos de Césio/toxicidade , Fragmentação do DNA/efeitos dos fármacos , Hemócitos/efeitos da radiação , Lymnaea/efeitos da radiação , Radioisótopos de Estrôncio/toxicidade , Poluentes Radioativos da Água/toxicidade , Animais , Proliferação de Células/efeitos dos fármacos , Radioisótopos de Césio/análise , Radioisótopos de Césio/metabolismo , Ensaio Cometa , Hemócitos/citologia , Hemócitos/metabolismo , Lymnaea/citologia , Lymnaea/metabolismo , República de Belarus , Software , Radioisótopos de Estrôncio/análise , Radioisótopos de Estrôncio/metabolismo , Poluentes Radioativos da Água/análise , Poluentes Radioativos da Água/metabolismoRESUMO
The age-related changes in hemolymph cellular composition of snail Lymnaea stagnalis (Gastropoda, Pulmonata) obtained from individuals of a natural population (the river Pripayt, Gomel region, Belarus) as well as in the spectrum of cytomorphological traits of hemocyte genetic damages have been studied. The percentage of the distinguished hemolymph cell types during the chosen age period was not revealed to change. The percentage of cells with different morphological attributes of cell death varied during ageing. The tendency to increase in the total level of dying cells was observed.