RESUMO
Pluronic-based core/shell nanoparticles (NPs) were formed using various strategies such as self-assembly and temperature induced-phase transition. To improve their functionality as a nanomedicine for diagnosis and therapy, the vesicle fusion and layer by layer approach were employed. Because of the hydrophilic nature of the Pluronic shell and the relatively small size, Pluronic-based core/shell NPs were used in order to improve their pharmacokinetic behaviors in drugs and in imaging agents. This review will introduce various types of Pluronic-based core/shell NPs according to their preparation method and formation mechanism. The focus will be on the Pluronic-based core/shell NPs for tumor targeting, stimulated release of proteins, and cancer imaging capabilities.
Assuntos
Portadores de Fármacos/química , Nanopartículas/química , Poloxâmero/química , Antineoplásicos/administração & dosagem , Antineoplásicos/química , Materiais Biocompatíveis/química , Humanos , Lipossomos/química , Micelas , Neoplasias/diagnóstico , Neoplasias/tratamento farmacológico , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/químicaRESUMO
The multilayer nanoparticles (NPs) were prepared for cancer-targeting therapy using the layer by layer approach. When drug-loaded Pluronic NPs were mixed with vesicles (liposomes) in the aqueous medium, Pluronic NPs were incorporated into the vesicles to form the vesicle NPs. Then, the multilayer NPs were formed by freeze-drying the vesicle NPs in a Pluronic aqueous solution. The morphology and size distribution of the multilayer NPs were observed using a TEM and a particle size analyzer. In order to apply the multilayer NPs as a delivery system for docetaxel (DTX), which is a model anticancer drug, the release pattern of the DTX was observed and the tumor growth was monitored by injecting the multilayer NPs into the tail veins of tumor (squamous cell carcinoma)-bearing mice. The cytotoxicity of free DTX (commercial DTX formulation (Taxotere®)) and the multilayer NPs was evaluated using MTT assay. We also evaluated the tumor targeting ability of the multilayer NPs using magnetic resonance imaging. The multilayer NPs showed excellent tumor targetability and antitumor efficacy in tumor-bearing mice, caused by the enhanced permeation and retention (EPR) effect. These results suggest that the multilayer NPs could be a potential drug delivery system for cancer-targeting therapy.