RESUMO
Methods of titration of the CS vaccine strain of classical swine fever virus were compared in vitro and vivo. The titration in the TL and PK-15 cell culture without cytopathic effect is based on the detection of virus antigen by labeled antibodies. The infection intensity in the cell culture virtually correlated with the antigenic and immunogenic activity of dry vaccine used for swine.
Assuntos
Vírus da Febre Suína Clássica/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Técnica Indireta de Fluorescência para Anticorpo , Vacinas Virais/normas , Animais , Anticorpos Monoclonais , Anticorpos Antivirais , Antígenos Virais/análise , Células Cultivadas , Peste Suína Clássica/prevenção & controle , Vírus da Febre Suína Clássica/imunologia , Injeções Intramusculares , Peroxidase , Ovinos , Suínos , Vacinas Virais/administração & dosagem , Vacinas Virais/imunologiaRESUMO
Several cell cultures were compared and examined for their capability to support reproduction of Classical Swine Fever Virus (CSFV). Experimental conditions of virus cultivation were optimized. The subculture of primary lamb testicular (LT) cells was selected for large cell cultivation of CSFV. In contrast to many cell lines, these cells were free of Bovine Viral Diarrhea Virus (BVDV) contamination partly due to liquid nitrogen storage and gamma-ray sterilization of serum used in the growth media. The vaccine strain CS of CSFV was obtained in the titers of 6.7-7.7 TCID50/ml under the optimal conditions of virus reproduction in the LT cells.