Melatonin Alleviates Ovariectomy-Induced Cardiovascular Inflammation in Sedentary or Exercised Rats by Upregulating SIRT1.

We aimed to evaluate the impact of hormone replacement, melatonin, or exercise alone or their combination on oxidative damage and functional status of heart, brain, and aorta of ovariectomized (OVX) rats and to determine whether the signaling pathway is dependent on sirtuin-1 (SIRT1). Ovariectomized Sprague Dawley rats were orally given either a hormone replacement therapy (1 mg/kg/day,17ß estradiol; HRT) or melatonin (4 mg/kg/day) or HRT + melatonin treatments or tap water, while each group was further divided into sedentary and exercise (30 min/5 days/week) groups. After the heart rate measurements and memory tests were performed, trunk blood was collected at the end of the 10th week to determine metabolic parameters in serum samples. Tissue samples of abdominal aorta, heart, and brain were taken for biochemical measurements and histopathological evaluation. Heart rates and memory performances of the OVX rats were not changed significantly by none of the applications. Melatonin treatment or its co-administration with HRT upregulated the expressions of IL-10 and SIRT1, reduced the expressions of IL-6 and TNF-α, and reduced DNA damage in the hearts and thoracic aortae of non-exercised rats. Co-administration of melatonin and HRT to exercised OVX rats reduced inflammatory response and upregulated SIRT1 expression in the aortic and cardiac tissues. The present study suggests that melatonin treatment, either alone or in combination with exercise and/or HRT, upregulates SIRT1 expression and alleviates oxidative injury and inflammation in the hearts and aortas of OVX rats. Melatonin should be considered in alleviating cardiovascular disease risk in postmenopausal women.

Bee pollens as biological indicators: An ecological assessment of pollution in Northern Turkey via ICP-MS and XPS analyses.

In this study, pollens were collected from 25 different locations of Northern Turkey to investigate pollution monitoring. Surface chemistry of pollen samples was characterized by X-ray photoelectron spectroscopy (XPS). Then the concentrations of certain elements (Li, Al, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Cd, Ba, and Pb) in pollen samples were determined by inductively coupled plasma mass spectrometry (ICP-MS) for the evaluation of environmental pollution. The levels of elements were detected in the following ranges (minimum-maximum, mg/kg dry pollen): Li (0.18-0.39), Al (24.98-308.04), V (6.18-98.58), Cr (1.05-6.81), Mn (13.85-95.91), Fe (52.20-326.26), Co (0.15-0.34), Ni (1.66-10.79), Cu (8.61-19.01), Zn (20.47-70.02), As (1.22-2.65), Se (0.39-0.67), Cd (0.05-0.74), Ba (0.73-16.30), and Pb (0.00-0.26). It has been concluded that there is a correlation between the pollen samples with high heavy metal concentrations and traffic density as these regions are closer to the road in the northern region. It is exposed to pollution from various sources such as intensified urbanization and tourism activities carried out on land and sea; industrial activities are increasing rapidly due to the opportunities offered by the coastal areas, sea transportation, and agricultural, domestic, and industrial pollution coming from the inner regions through rivers and streams. In this sense, pollens can be used as potential bio-indicators for monitoring heavy metal pollution and gives an idea about how we can use them for future assessing purposes.