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Elife ; 102021 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-33890575

RESUMO

Mechanical stress during cell migration may be a previously unappreciated source of genome instability, but the extent to which this happens in any animal in vivo remains unknown. We consider an in vivo system where the adult stem cells of planarian flatworms are required to migrate to a distal wound site. We observe a relationship between adult stem cell migration and ongoing DNA damage and repair during tissue regeneration. Migrating planarian stem cells undergo changes in nuclear shape and exhibit increased levels of DNA damage. Increased DNA damage levels reduce once stem cells reach the wound site. Stem cells in which DNA damage is induced prior to wounding take longer to initiate migration and migrating stem cell populations are more sensitive to further DNA damage than stationary stem cells. RNAi-mediated knockdown of DNA repair pathway components blocks normal stem cell migration, confirming that active DNA repair pathways are required to allow successful migration to a distal wound site. Together these findings provide evidence that levels of migration-coupled-DNA-damage are significant in adult stem cells and that ongoing migration requires DNA repair mechanisms. Our findings reveal that migration of normal stem cells in vivo represents an unappreciated source of damage, which could be a significant source of mutations in animals during development or during long-term tissue homeostasis.


Assuntos
Células-Tronco Adultas/patologia , Movimento Celular , Dano ao DNA , Reparo do DNA , Planárias , Cicatrização , Células-Tronco Adultas/metabolismo , Células-Tronco Adultas/efeitos da radiação , Animais , Movimento Celular/efeitos da radiação , Forma do Núcleo Celular , Regulação da Expressão Gênica , Instabilidade Genômica , Cinética , Planárias/genética , Planárias/metabolismo , Planárias/efeitos da radiação , Estresse Mecânico , Cicatrização/efeitos da radiação
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