[Effect of various xenobiotics on the in vivo incorporation of 14C-acetate into cholesterol and other lipids in the rat]. / Die Wirkung einiger Fremdstoffe auf den in vivo-Einbau von [14C]-Azetat in Cholesterol und andere Lipide der Ratte.

Effects of some xenobiotics on the incorporation in vivo of [14C]-acetate into cholesterol and other lipids of the rat DDT, HCH, polychlorinated biphenyl, chlorcholine chloride, ethylenthiourea and mixtures of these substances were given to rats with the feed for 7 days. After that the incorporation of [14C]-acetate into phospholipids, triglycerides and cholesterol was studied in liver, serum, heart and brain. The influence of the xenobiotics on the synthesis, transport and accumulation differed among the various organs and lipids. Outstanding was an increase of 14C-phospholipids, -triglycerides and -cholesterol in the heart after administration of polychlorinated biphenyl. The effects in the peripheral organs were generally more distinct than on the liver.

[Effect of the phosphonic acid derivatives ethephon and trichlorphon on the incorporation in vivo of (14C)-acetate into cholesterol and other lipids in rats]. / Einfluss der Phosphonsäurederivate Ethephon und Trichlorphon auf den in vivo-Einbau von [14C]-Azetat in Cholesterol und andere Lipide der Ratte.

The effect of the phosphonic acid derivatives Ethephon and Trichlorphon on the incorporation of 14C-labelled acetate into lipids especially cholesterol was investigated. Adult Wistar rats were fed for 7 days diets containing 50 and 500 ppm, respectively, of the phosphonic acid derivatives. Both compounds caused a significant increase of the 14C-activity in lipids of serum, liver, heart, and brain. The effect of Ethephon was significantly more intense than that of Trichlorphon. The important finding was the Ethephon-induced increase of the [14C]-acetate incorporation into cholesterol which continued across all the tissues studied.

[Method for the control of (rat) liver functions by means of 14C-acetate for the toxicological analysis of foreign substances]. / Methode zur Kontrolle von (Ratten-)Leberfunktionen mit Hilfe von 14-C-Acetat für die toxikologische Untersuchung von Fremdstoffen.

The authors present a test procedure which permits to obtain, by means of a single carbon-14-labelled compound as structural element in a series of essential substances, a survey of important fields of liver function. Special attention was paid to cholesterol. Methodical studies resulted in an optimization of the application under in vivo and in vitro conditions, and of the isolation of carbon-14-labelled cholesterol, ester cholesterol and glucose as well as of carbon-14-labelled phospholipids, triglycerides, bile acids, proteins and nucleic acids as groups. A comparison of the experimental techniques demonstrated the advantages of in vivo application. The method and its validity are illustrated by an example (DDT). Its use in studies on various active principles will be dealt with in future communications.