Combined treatment of sinapic acid and ellagic acid attenuates hyperglycemia in streptozotocin-induced diabetic rats.

In the present study, we aimed to investigate the effect of individual and combined treatment of sinapic acid (SA) and ellagic acid (EA) in streptozotocin (STZ)-induced diabetic rats. Rats were divided into eight groups (n = 7): Normal Control, Diabetic Control, Diabetic + Sinapic Acid, Diabetic + Ellagic Acid, Diabetic + Sinapic Acid + Ellagic Acid, Sinapic Acid, Ellagic Acid and Sinapic Acid + Ellagic Acid. Diabetic groups were injected with a single dose of 50 mg/kg STZ intraperitoneally. Rats received 20 mg/kg/day SA and 50 mg/kg/day EA intragastrically for 28 days. The numerical density of immunopositive ß-cells and volume density of pancreatic islets were calculated. Additionally, glucose and insulin levels in serum, MDA, GSH, and CAT levels of pancreatic tissue were measured. While serum glucose levels increased, serum insulin levels decreased in STZ-induced diabetic rats. But these changes in glucose and insulin were restored by individual and combined treatments of SA and EA. Also, individual and combined treatments of SA and EA increased insulin expression of ß-cells in STZ-induced diabetic rats. Moreover, these compounds improved deteriorating oxidative stress parameters in STZ-induced diabetic rats. Our study indicates that SA and EA, especially their combined treatments, can be used as an antihyperglycemic agent in diabetes.

Prenatal exposure to low-dose diclofenac sodium does not affect total neuron numbers in spinal segment T13 in rats.

The main purpose of the present study was to investigate the effects of diclofenac sodium (DS) on the total number of neurons in segment T13 of the spinal cord of offspring of pregnant rats using stereological methods. Eighteen adult female Wistar albino rats weighing 150-200g were used. Pregnant female rats were divided into three groups; a control group, a sham group and a DS (1mg/kg, intramuscular) exposed group. The DS and sham groups received injection from the 5th day of gestation to the 19th. Twenty eight days after birth, the offspring rats were perfused with 4% buffered formalin. T13, which is one of transverse spinal cord segments, were isolated and processed for routine paraffin histology. 5µm sections were obtained using a rotary microtome according to systematic random sampling strategies. Every 40th section was taken and sections were stained with modified Giemsa. All types of motor neuron cell were identified according to their morphology. In this study, the "disector-Cavalieri combination" method was used in the stereological examination of neurons. The motor neurons were counted in the right gray matter of the ventral horn in the spinal cord segment. The Kruskal-Wallis test was used for comparison the groups. In terms of motoneuron number, no significant difference among the groups was found (p>0.05). In conclusion, our results indicated that prenatal exposure to DS has no effect on the total number of motor neuron of the offspring rats.

Aperture in coronoid-olecranon septum: A radiological evaluation.

BACKGROUND: The humerus bone with coronoid-olecranon septal aperture have very narrow medullary canal and extreme anterolateral bowing proximally and/or extreme anterolateral compressed surface at distal part. Septal perforation might make nailing through a retrograde technique more difficult or possibly with an increased risk of iatrogenic fracture at the site of insertion. Coronoid-olecranon perforation might be very important in preoperative planning for successful nailing. Since the data available has been obtained from the ancient studies using the skeletal material, the present study is aimed at investigating originally the presence of the coronoid-olecranon septum perforation in the living subjects, using a radiographic method. MATERIALS AND METHODS: The study was performed on direct radiograms and computed tomography of the elbow obtained from the 709 volunteers (367 males, 342 females). RESULTS: The incidence of the perforation was found to be 8.6 %. Bilateral occurrence was found to be more common significantly. The highest incidence occurred in second decade. CONCLUSION: The present study suggests female prepronounced and left asymmetry in case of septal perforation. Bilateral occurrence from our study might be peculiar to the clinician.

A morphometric study on dog cerebellum.

In healthy humans and patient with schizophrenia, studies investigating cerebellar asymmetries have been performed by using magnetic resonance images (MRIs), which have failed to demonstrate the presence of the left-right asymmetry. Due to the paucity of animal studies in this field, the present study was undertaken to investigate whether the dog cerebellum shows any pattern of volumetric asymmetry. A total of 16 adult mongrel dogs, 8 male and 8 female, were assessed with respect to pawedness. After the animals were anesthetized and killed by exsanguinations, the cerebellum was removed and divided into the anterior and the posterior lobes by a dissection passing into the fissure prima. The volumes of the right and left hemispheres were separately calculated by a volumetric device. The present study demonstrated significant volume asymmetries as to right-left, sex and pawedness in dog cerebellum. Morphological asymmetries in dog cerebellum might relate to cerebral specialization of function. Further studies need to be performed to demonstrate the relationship between cerebellar asymmetries and functional lateralization.

The effect of mobile phone on the number of Purkinje cells: a stereological study.

PURPOSE: The World Health Organisation proposed an investigation concerning the exposure of animals to radiofrequency fields because of the possible risk factor for health. At power frequencies there is evidence to associate both childhood leukaemia and brain tumours with magnetic field exposures. There is also evidence of the effect of mobile phone exposure on both cognitive functions and the cerebellum. Purkinje cells of the cerebellum are also sensitive to high dose microwave exposure in rats. The present study investigated the effect of exposure to mobile phone on the number of Purkinje and granule neurons in the developing cerebellum. MATERIAL AND METHODS: Male and female Swiss albino mice were housed as control and mobile phone-exposed groups. Pregnant animals in the experimental group were exposed to Global System for Mobile Communication (GSM) mobile phone radiation at 890-915 MHz at 0.95 W/Kg specific absorption rate (SAR). The cerebella were processed by frozen microtome. The sections obtained were stained with Haematoxylin-eosin and cresyl violet. For cell counting by the optical fractionator method, a pilot study was firstly performed. Cerebellar areas were analysed by using Axiovision software running on a personal computer. The optical dissectors were systematically spaced at random, and focused to the widest profile of the neuron cell nucleus. RESULTS: A significant decrease in the number of Purkinje cells and a tendency for granule cells to increase in cerebellum was found. CONCLUSION: Further studies in this area are needed due to the popular use of mobile telephones and relatively high exposure on developing brain.

Total neuron numbers in CA1-4 sectors of the dog hippocampus.

BACKGROUND & OBJECTIVES: Early reports addressed morphological asymmetry in the cross-sectional width of the rat hippocampus. The present study was aimed at counting total number of neurons in CA1-4 sectors and the subiculum of the dog hippocampus as well as investigating possible left /right and male/female asymmetry. METHODS: Adult mongrel dogs (8 female and 5 male) were assessed by the right and left pawedness and sacrificed by exsanguinations. In each hippocampus dissected, the total neuron numbers of CAs and subiculum were estimated by the physical fractioning method. RESULTS: Significant hemispheric asymmetries were found in the number of pyramidal cells of CA1, CA3/2, CA4 and the subiculum. Sex difference was also found in the subiculum, in favour of the males. INTERPRETATION & CONCLUSION: Our study indicated a left dominant asymmetry in males and right dominancy in females as well as no functional asymmetry in specific regions of the dog hippocampus. Further investigations are necessary to verify the hypothesis that hippocampal morphological asymmetries in normal subjects are functionally related in memory or in cognitive skills.

Effect of prenatal exposure to mobile phone on pyramidal cell numbers in the mouse hippocampus: a stereological study.

Because of the possible risk factor for the health, World Health Organization (WHO) recommended the study with animals on the developing nervous system concerning the exposure to radiofrequency (RF) field. A few studies related to hippocampal exposure are available, which indicate the impact of RF field in some parameters. The present study investigated the effect of exposure to mobile phone on developing hippocampus. Male and female Swiss albino mice were housed as control and mobile phone exposed groups. The pregnant animals in tested group were exposed to the effects of mobile phone in a room possessing the exposure system. The left hemispheres of the brains were processed by frozen microtome. The sections obtained were stained with Hematoxylin & Eosin. For cell counting by the optical fractionator method, a pilot study was first performed. Hippocampal areas were analyzed using Axiovision software running on a personal computer. The optical dissector, systematically and randomly spaced, was focused to the widest profile of the pyramidal cell nucleus. No significant difference in pyramidal cell number of total Cornu Ammonis (CA) sectors of hippocampus was found between the control and the mobile phone exposed groups (p > .05). It was concluded that further study is needed in this field due to popular use of mobile telephones and relatively high exposure to the developing brain.