NANOPARTICLES AND COLORECTAL CANCER: CAN THE USE OF METAL NANOPARTICLE COMPOSITIONS AFFECT OXIDATIVE STRESS MARKERS AND COLON HISTOLOGICAL CHANGES UNDER DMH-INDUCED CARCINOGENESIS.

Colorectal cancer (CRC) - a significant global health challenge. Exploring biological markers of oxidative stress is crucial, as they can play an essential role in initiating the transition from an organ's "healthy state" to a "malignant injury." There is substantial promise in investigating the level of 8-isoprostane (8-isoPGF2α) as a novel and dependable marker of oxidative stress. This paper presents that 8-isoprostane levels have been linked to the development of severe structural changes in the colon wall, accompanied by endogenic intoxication syndrome. The obtained results prove the strong connection between oxidative stress and carcinogenesis progression. Our research further illustrates the favorable and potentially beneficial impact of the Au/Ag/Fe NPs composition, which can find utility in a diverse range of contemporary applications.

EFFECT OF XENOGRAFTS SATURATED WITH SILVERNANOCRYSTALS ON HISTOLOGICAL STRUCTURE OF THE SKIN IN THE DYNAMICS OF EXPERIMENTAL THERMAL INJURY.

The aim of this study was to establish histological state of the components of the affected area of skin in cases of application of xenograft saturated with silver nanocrystals in the dynamics after experimental thermal injury. The study was performed on 54 adult guinea pigs following the rules of bioethics. Experimental animals were divided into two groups: the 1st - animals with severe thermal injury (27); the 2nd - animals with burn injury (27), in which the wounds were covered with xenograft after early necrectomy of the damaged tissues. To investigate the microscopic changes in the skin the animals were removed from the experiment on the 7th, 14th, and 21st days of the experiment. Histological sections were stained with hematoxylin and eosin. Application of xenograft saturated with silver nanocrystals for 7 days contributes to formation of granulation tissue, activation of regenerative mechanisms in the marginal areas of the wound. On the 14th day of the experiment, an epidermal regenerate was evidenced at the wound, its formation source was the perifocal areas of the epidermis and skin appendages, and below it - fibroblastic cells rich granulation tissue, well-defined fibrous structures, equally set components of the hemomicrocirculatory flow. On the 21st day, a well-formed epidermis with a clear differentiation of cells into layers was observed. In the fresh connective tissue, formation of papillae with new capillaries embedded in the epithelium was evidenced. There were collagen as well as elastic fibres among the fibrous structures of the intercellular substance of the connective tissue. In all stages of the experiment with application of xenograft saturated with silver nanocrystals no purulonecrotic masses were observed in the affected area. Thus, histologically it has been found out that application of xenograft saturated with silver nanocrystals after early necrectoна руссmy of burn-damaged areas of the skin at an early stage improves formation of granulation tissue. In the late post-experimental thermal injuries, their usage accelerates epithelialization, connective tissue formation and has positive effect on the course of the experimental burn wound process.

[MORPHOLOGICAL CHANGES IN THE LIVER OF MICE WITH ANTIPHOSPHOLIPID SYNDROME AND ADMINISTRATION OF NITRIC OXIDE SYNTHESIS MODULATORS].

The aim of the research is to study the effect of L-arginine, the precursor of nitric oxide, and aminoguanidine, the inhibitor of inducible nitric oxide synthase, on morpho-functional changes in the liver of BALB/c mice with antiphospholipid syndrome. The study was carried out on 50 female BALB/c mice modelled with antiphospholipid syndrome. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for its correction. The material for microscopic study was taken by the method of Horalsky. The liver tissue samples were stained with hematoxylin and eosin. Significant hemodynamic disorders with manifestations of thrombosis in the liver in cases of antiphospholipid syndrome followed by destructive-degenerative changes of the stoma and parenchyma have been established. Administration of L-arginine in antiphospholipid syndrome caused restoration of the lobular and beam organization of the liver. The maximum effect on the morphological state of the liver was observed in using a combination of L-arginine and aminoguanidine. The protective effect of L-arginine and aminoguanidine on the microcirculation has been proved that evidences liver function restoration in mice with antiphospholipid syndrome.

[Status of cell surface of hepatocytes after exposure to carbon tetrachloride]. / Sostoianie kletochnoi poverkhnosti gepatotsitov pri deistvii chetyrekhkhloristogo ugleroda.

In order to estimate contact interactions and deformity of the surface of hepatocytes at a toxic hepatitis, produced by means of carbon tetrachloride, a morphometric analysis of ultrastructures of the contacts and retractions of the surface of hepatocytes, formed at an increased intraorganic pressure has been performed. Carbon tetrachloride produces decrease in adhesive interactions of the cellular surfaces in the area of simple connections, decrease in amount of desmosomes and results in increasing deformity of the surface of hepatocytes. These data support that there exists a universal reaction of the intercellular contacts to a damaging action.