Hyper pooling private trips into high occupancy transit like attractive shared rides.

The size of the solution space associated with the trip-matching problem has made the search for high-order ride-pooling prohibitive. We introduce hyper-pooled rides along with a method to identify them within urban demand patterns. Travellers of hyper-pooled rides walk to common pick-up points, travel with a shared vehicle along a sequence of stops and are dropped off at stops from which they walk to their destinations. While closely resembling classical mass transit, hyper-pooled rides are purely demand-driven, with itineraries (stop locations, sequences, timings) optimised for all co-travellers. For 2000 trips in Amsterdam the algorithm generated 40 hyper-pooled rides transporting 225 travellers. They would require 52.5 vehicle hours to travel solo, whereas in the hyper-pooled multi-stop rides, it is reduced sixfold to 9 vehicle hours only. This efficiency gain is made possible by achieving an average occupancy of 5.8 (and a maximum of 14) while remaining attractive for all co-travellers.

The shareability potential of ride-pooling under alternative spatial demand patterns.

In In this study, we set out to explore how various spatial patterns of travel demand drive the effectiveness of ride-pooling services. To do so, we generate a broad range of synthetic, yet plausible demand patterns. We experiment with the number of attraction centres, the dispersion of destinations around these centres, and the trip length distribution. We apply a strategic ride-pooling algorithm across the generated demand patterns to identify shareability potential using a series of metrics related to ridepooling. Our findings indicate that, under a fixed demand level, vehicle-hour reduction due to ride-pooling can range between 18 and 59%. These results depend on the concentration of travel destinations around the centre and the trip length distribution. Ride-pooling becomes more efficient when trips are longer and destinations are more concentrated. A shift from a monocentric to a polycentric demand pattern is found to have a limited impact on the prospects of ride-pooling.

Mitigating bus bunching with real-time crowding information.

A common problem in public transport systems is bus bunching, characterized by a negative feedback loop between service headways, number of boarding passengers and dwell times. In this study, we examine whether providing real-time crowding information (RTCI) at the stop regarding the two next vehicle departures can stimulate passengers to wait for a less-crowded departure, and thus alleviate the bunching effect. To this end, we leverage on results from own stated-preference survey and develop a boarding choice model. The model accounts for the presence of RTCI and is implemented within dynamic public transport simulation framework. Application to the case-study model of a major bus corridor in Warsaw (Poland) reveals that RTCI can induce a significant probability (30-70%) of intentionally skipping an overcrowded bus and waiting for a later departure instead. This behaviour, in turn, results in significantly lower vehicle headway and load variations, without deteriorations in total waiting utility. Overall, journey experience improves by 6%, and crucially-the prevalence of denial-of-boarding and excessive on-board overcrowding is substantially reduced, by ca. 40%. Results of our study indicate that the willingness to wait induced by RTCI can be a potential demand management strategy in counteracting bunching, with benefits already attainable at limited RTCI response rates.

Simulating two-sided mobility platforms with MaaSSim.

Two-sided mobility platforms, such as Uber and Lyft, widely emerged in the urban mobility landscape. Distributed supply of individual drivers, matched with travellers via intermediate platform yields a new class of phenomena not present in urban mobility before. Such disruptive changes to transportation systems call for a simulation framework where researchers from various and across disciplines may introduce models aimed at representing the complex dynamics of platform-driven urban mobility. In this work, we present MaaSSim, a lightweight agent-based simulator reproducing the transport system used by two kinds of agents: (i) travellers, requesting to travel from their origin to destination at a given time, and (ii) drivers supplying their travel needs by offering them rides. An intermediate agent, the platform, matches demand with supply. Agents are individual decision-makers. Specifically, travellers may decide which mode they use or reject an incoming offer; drivers may opt-out from the system or reject incoming requests. All of the above behaviours are modelled through user-defined modules, allowing to represent agents' taste variations (heterogeneity), their previous experiences (learning) and available information (system control). MaaSSim is a flexible open-source python library capable of realistically reproducing complex interactions between agents of a two-sided mobility platform. MaaSSim is available from a public repository, along with a set of tutorials and reproducible use-case scenarios, as demonstrated with a series of illustrative examples and a comprehensive case study.

The development of cigarette smoke induced chronic pancreatitis in mice is associated with increased expression of K-Ras and NF-κB.

INTRODUCTION AND OBJECTIVE: Epidemiological studies have demonstrated a strong association between cigarette smoking (CS) and chronic pancreatitis (CP); however, the exact mechanisms of this phenomenon remains unknown. The authors have previously shown that increased Ras expression activates the NF-κB mediated pathway and promotes development of CP. However, it is unclear whether a similar phenomenon occurs in CS-induced CP. Therefore, the aim of the study was to determine whether CS increases the expression of K-Ras, and promotes the development of CP in mice exposed to repeated episodes of acute pancreatitis (AP). MATERIAL AND METHODS: C57BL6/cmdb mice were exposed to CS or a sham treatment for 12 weeks. After one week of exposure, half of the animals from both groups were additionally subjected to repeated cerulein treatment (once a week, for 10 consecutive weeks) to mimic recurrent episodes of AP. Extension of pancreatic damage was determined histologically by H&E and Trichrome staining. The expression of K-Ras protein and downstream components (NF-κB, Cox-2, TGF-ß) was evaluated by immunohistochemistry. RESULTS: C57BL6/cmdb mice exposed to CS or cerulein alone did not develop any chronic pancreatic damage. However, concomitant treatment with both of these agents caused focal acinar atrophy, with slight intralobular and perivascular areas of fibrosis, and inflammatory cells infiltration resembling mild CP. Moreover, immunohistochemistry examinations revealed increased pancreatic expression of K-Ras and NF-κB only in mice treated both with CS and cerulein. CONCLUSIONS: CS promotes development of CP in mice exposed to repeated episodes of AP. This process may be, at least partially, related to increased expression of K-Ras and NF-κB protein.

Beyond the dichotomy: How ride-hailing competes with and complements public transport.

Since ride-hailing has become an important travel alternative in many cities worldwide, a fervent debate is underway on whether it competes with or complements public transport services. We use Uber trip data in six cities in the United States and Europe to identify the most attractive public transport alternative for each ride. We then address the following questions: (i) How does ride-hailing travel time and cost compare to the fastest public transport alternative? (ii) What proportion of ride-hailing trips do not have a viable public transport alternative? (iii) How does ride-hailing change overall service accessibility? (iv) What is the relation between demand share and relative competition between the two alternatives? Our findings suggest that the dichotomy-competing with or complementing-is false. Though the vast majority of ride-hailing trips have a viable public transport alternative, between 20% and 40% of them have no viable public transport alternative. The increased service accessibility attributed to the inclusion of ride-hailing is greater in our US cities than in their European counterparts. Demand split is directly related to the relative competitiveness of travel times i.e. when public transport travel times are competitive ride-hailing demand share is low and vice-versa.

Modelling virus spreading in ride-pooling networks.

Urban mobility needs alternative sustainable travel modes to keep our pandemic cities in motion. Ride-pooling, where a single vehicle is shared by more than one traveller, is not only appealing for mobility platforms and their travellers, but also for promoting the sustainability of urban mobility systems. Yet, the potential of ride-pooling rides to serve as a safe and effective alternative given the personal and public health risks considerations associated with the COVID-19 pandemic is hitherto unknown. To answer this, we combine epidemiological and behavioural shareability models to examine spreading among ride-pooling travellers, with an application for Amsterdam. Findings are at first sight devastating, with only few initially infected travellers needed to spread the virus to hundreds of ride-pooling users. Without intervention, ride-pooling system may substantially contribute to virus spreading. Notwithstanding, we identify an effective control measure allowing to halt the spreading before the outbreaks (at 50 instead of 800 infections) without sacrificing the efficiency achieved by pooling. Fixed matches among co-travellers disconnect the otherwise dense contact network, encapsulating the virus in small communities and preventing the outbreaks.

Untargeted Metabolomics and Inflammatory Markers Profiling in Children With Crohn's Disease and Ulcerative Colitis-A Preliminary Study.

BACKGROUND: Metabolic profiling might be used to identify disease biomarkers. The aim of our study was to determine the usefulness of untargeted metabolomics analysis to detect differences in serum metabolites between newly diagnosed and untreated pediatric patients with Crohn's disease (CD) or ulcerative colitis (UC) in comparison with a control group (Ctr). Moreover, we investigated the potential of profiling metabolomics and inflammatory markers to improve the noninvasive diagnosis of CD and UC in children. METHODS: Metabolic fingerprinting of serum samples was estimated with liquid chromatography coupled with mass spectrometry in children with CD (n = 9; median age, 14 years), UC (n = 10; median age, 13.5 years), and controls (n = 10; median age, 12.5 years). RESULTS: The majority of chemically annotated metabolites belonged to phospholipids and were downregulated in CD and UC compared with the Ctr. Only 1 metabolite, lactosylceramide 18:1/16:0 (LacCer 18:1/16:0), significantly discriminated CD from UC patients. Interestingly, combining LacCer 18:1/16:0 with other inflammatory markers resulted in a significant increase in the area under the curve with the highest specificity and sensitivity. CONCLUSIONS: Using serum untargeted metabolomics, we have shown that LacCer 18:1/16:0 is a very unique metabolite for CD patients.

Remediation aspect of microbial changes of plant rhizosphere in mercury contaminated soil.

Phytoremediation, an approach that uses plants to remediate contaminated soil through degradation, stabilization or accumulation, may provide an efficient solution to some mercury contamination problems. This paper presents growth chamber experiments that tested the ability of plant species to stabilize mercury in soil. Several indigenous herbaceous species and Salix viminalis were grown in soil collected from a mercury-contaminated site in southern Poland. The uptake and distribution of mercury by these plants were investigated, and the growth and vitality of the plants through a part of one vegetative cycle were assessed. The highest concentrations of mercury were found at the roots, but translocation to the aerial part also occurred. Most of the plant species tested displayed good growth on mercury contaminated soil and sustained a rich microbial population in the rhizosphere. The microbial populations of root-free soil and rhizosphere soil from all species were also examined. An inverse correlation between the number of sulfur amino acid decomposing bacteria and root mercury content was observed. These results indicate the potential for using some species of plants to treat mercury contaminated soil through stabilization rather than extraction. The present investigation proposes a practical cost-effective temporary solution for phytostabilization of soil with moderate mercury contamination as well as the basis for plant selection.

Effect of chemophytostabilization practices on arbuscular mycorrhiza colonization of Deschampsia cespitosa ecotype Warynski at different soil depths.

The effects of chemophytostabilization practices on arbuscular mycorrhiza (AM) of Deschampsia cespitosa roots at different depths in soils highly contaminated with heavy metals were studied in field trials. Mycorrhizal parameters, including frequency of mycorrhization, intensity of root cortex colonization and arbuscule abundance were studied. Correlations between concentration of bioavailable Cd, Zn, Pb and Cu in soil and mycorrhizal parameters were estimated. An increase in AM colonization with increasing soil depth was observed in soils with spontaneously growing D. cespitosa. A positive effect of chemophytostabilization amendments (calcium phosphate, lignite) on AM colonization was found in the soil layers to which the amendments were applied. Negative correlation coefficients between mycorrhizal parameters and concentration of bioavailable Cd and Zn in soil were obtained. Our results demonstrated that chemophytostabilization practices enhance AM colonization in D. cespitosa roots, even in soils fertilized with high rates of phosphorus.

A method of mercury removal from topsoil using low-thermal application.

Mercury contamination in the environment is problematic due to the unusual physical properties and well-recognized toxicity of this common metal. The bioavailability of mercury depends strongly on its chemical speciation. Anthropogenic mercury and its compounds appear in soil as "hot spots" located close to industrial facilities that used or produced mercury. The nature of the chemical production process, transportation and disposal practices often determined the chemical composition and distribution of mercury in the surrounding soils. Current ex situ soil remediation methods are expensive, produce undesirable side effects to the environment and usually involve transportation of contaminated soil. In this project, sponsored by the U.S. Department of Energy, a low-cost, simple approach to removing mercury from soil was evaluated. The process uses low-temperature thermal desorption of volatile metallic mercury and its compounds, and subsequent vapor capture. The project consisted of laboratory and plot-scale experiments. The laboratory efforts evaluated theoretical calculations of mercury removal as a function of time and temperature. The plot-scale experiment was a practical application of the laboratory results. For both experiments, mercury-polluted soil was obtained from a chemical production facility located in southern Poland. In laboratory experiments, at temperature 373 K total mercury concentration decreased in soil by nearly 32%. In plot-scale experiments, at temperature 440 K, about 60-70% of total mercury was removed from the soil. At the end of the experiment, a test of soil biological activity was performed to check if the high temperature applied to the soil did not impair the soil growth properties. There was no negative effect of temperature found.

Optimizing phytoremediation of heavy metal-contaminated soil by exploiting plants' stress adaptation.

Soil phytoextraction is based on the ability of plants to extract contaminants from the soil. For less bioavailable metals, such as Pb, a chelator is added to the soil to mobilize the metal. The effect can be significant and in certain species, heavy metal accumulation can rapidly increase 10-fold. Accumulation of high levels of toxic metals may result in irreversible damage to the plant. Monitoring and controlling the phytotoxicity caused by EDTA-induced metal accumulation is crucial to optimize the remedial process, i.e. to achieve maximum uptake. We describe an EDTA-application procedure that minimizes phytotoxicity by increasing plant tolerance and allows phytoextraction of elevated levels of Pb and Cd. Brassica juncea is tested in soil with typical Pb and Cd concentrations of 500 mg kg-1 and 15 mg kg-1, respectively. Instead of a single dose treatment, the chelator is applied in multiple doses, that is, in several small increments, thus providing time for plants to initiate their adaptation mechanisms and raise their damage threshold. In situ monitoring of plant stress conditions by chlorophyll fluorescence recording allows for the identification of the saturating heavy metal accumulation process and of simultaneous plant deterioration.

Human health risk assessment case study: an abandoned metal smelter site in Poland.

United States Environmental Protection Agency methodologies for human health risk assessment (HRA) were applied in a Brownfields Demonstration Project on the Warynski smelter site (WSS), an abandoned industrial site at Piekary Slaskie town, Upper Silesia, Poland. The HRA included the baseline risk assessment (BRA) and the development of risk-based preliminary remedial goals (RBPRGs). The HRA focused on surface area covered with waste materials, which were evaluated with regard to the potential risks they may pose to humans. Cadmium, copper, iron, manganese, lead, and zinc were proposed as the contaminants of potential concern (COPCs) at WSS based on archive data on chemical composition of waste located on WSS. For the defined future land use patterns, the industrial (I) and recreational (II) exposure scenarios were assumed and evaluated. The combined hazard index for all COPCs was 3.1E+00 for Scenario I and 3.2E+00 for Scenario II. Regarding potential carcinogenic risks associated with the inhalation route, only cadmium was a contributor, with risks of 1.6E-06 and 2.6E-07 for Scenario I and Scenario II, respectively. The results of the BRA indicated that the potential health risks at WSS were mainly associated with waste material exposure to cadmium (industrial and recreational scenarios) and lead (industrial scenario). RBPRGs calculated under the industrial scenario were 1.17E+03 and 1.62E+03 mg/kg for cadmium and lead, respectively. The RBPRG for cadmium was 1.18E+03 mg/kg under the recreational scenario. The BRA results, as well as RBCs, are comparable for both scenarios, so it is impossible to prioritize land use patterns for WSS based on these results. For choosing a future land use pattern or an appropriate redevelopment option, different factors would be decisive in the decision-making process, e.g., social, market needs, technical feasibility and costs of redevelopment actions or acceptance of local community.