Enhanced photocatalytic and SERS performance of Ag nanoparticles functionalized MoS2 nanoflakes.

We report the preparation of Ag nanoparticles functionalized MoS2 nanoflakes by using the chemical reduction method followed by the hydrothermal method. Field emission scanning electron microscopy and elemental mapping reveals the uniform functionalization of Ag nanoparticles with MoS2 nanoflakes. High density of Ag plasmonic nanoparticles onto MoS2 nanoflakes demonstrates tremendously improved charge separation behavior in Ag-MoS2 nanohybrids. Photodecomposition capability of plasmonic Ag-MoS2 nanohybrids was explored by the decomposition of industrial pollutant molecules, showing a direct correlation between the Ag content over the MoS2 surface with their photodecomposition ability. The SERS-based detection profiles of the plasmonic were investigated by the ultra-low detection of MB molecules. The Ag-MoS2 nanohybrids SERS substrate manifests the detection of MB molecules solution up to a concentration of 10-9 M with an enhancement factor of 107. In the current study, we proposed and elucidated the probable efficient charge transfer mechanism for improved photocatalytic behavior and SERS-based sensing performance.

Impact of nanomaterials on sustainable pretreatment of lignocellulosic biomass for biofuels production: An advanced approach.

Biomass to biofuels production technology appears to be one of the most sustainable strategies among various renewable energy resources. Herein, pretreatment is an unavoidable and key step to increase free cellulose availability and digestibility to produce green fuels. Various existing pretreatment technologies of lignocellulosics biomasses (LCBs) face distinct challenges e.g., energy consuming, cost intensive, may lead partial removal of lignin, complex inhibitors production as well as may cause environmental pollutions. These, limitations may be overcome with the application of nanomaterials, employed as nanocatalysts during the pretreatment process of LCBs. In this prospect, the present review focuses and summarizes results of numerous studies and exploring the utilizations of magnetic, carbon based nanostructure, and nanophotocatalysts mediated pretreatment processes along with their possible mechanisms to improve the biofuels production compared to conventional chemical based pretreatment approaches. Furthermore, different aspects of nanomaterials based pretreatment methods with their shortcomings and future prospects have been discussed.

Effect of microfluidization on quality characteristics of sapodilla (Manilkara achras L.) juice.

Various oxidative enzymes account for the quality degradation of sapodilla (Manilkara achras L.) juice and need to be inactivated through emerging and continuous green pressure processing technology. In this study, pressurization of sapodilla juice was attempted via microfluidization (MF) at pressure range of 10,000-30,000 pound per square inch (psi) with 1-3 passes or cycles. The impact of microfluidization on the activity of polyphenol oxidase (PPO), peroxidase (POD), color, total soluble solid (TSS), viscosity, serum cloudiness along with particle size, and microbial load of sapodilla juice was assessed. Results showed that microfluidization (MF) decreased the residual PPO activity from 100 to 80.78 % and POD activity from 100 to 40.57%. However, TSS (18.81-19.01 %), viscosity (2.64-2.06 cP), serum cloudiness (2.19-1.22 %) and total color change (3.19-18.54) was also significantly affected. Most of these changes were observed due to particle size (PS) reduction that varied from 65.19 to 8.13 µm. Microfluidized juice revealed color improvement at particular MF pressure and pass due to enzyme inactivation. Moreover, lowest microbial load (2.89 Log CFU/ mL) was found at 30,000 psi/3 pass of MF as compared to control sample (unprocessed juice) (7.57 Log CFU/ mL). Consequently, MF can be potential candidate in processing of juices against spoilage.

Biochar production with amelioration of microwave-assisted pyrolysis: Current scenario, drawbacks and perspectives.

In recent years, biomass has been reported to obtain a wide range of value-added products. Biochar can be obtained by heating biomass, which aids in carbon sinks, soil amendments, resource recovery, and water retention. Microwave technology stands out among various biomass heating technologies not only for its effectiveness in biomass pyrolysis for the production of biochar and biofuel but also for its speed, volumetrics, selectivity, and efficiency. The features of microwave-assisted biomass pyrolysis and biochar are briefly reviewed in this paper. An informative comparison has been drawn between microwave-assisted pyrolysis and conventional pyrolysis. It focuses mainly on technological and economic scenario of biochar production and environmental impacts of using biochar. This source of knowledge would aid in the exploration of new possibilities and scope for employing microwave-assisted pyrolysis technology to produce biochar.

Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases.

Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology. Supplementary Information: The online version contains supplementary material available at 10.1007/s40097-022-00472-7.

Synthesis and characterisation of zinc oxide modified biorenewable polysaccharides based sustainable hydrogel nanocomposite for Hg2+ ion removal: Towards a circular bioeconomy.

Industrial metal ion pollution has been considered the chief source of water contaminants all over the world. In the present research, we have prepared gum tragacanth cross-linked 2-hydroxyethyl methacrylate-co-acrylamide (GT-cl-(HEMA-co-AAm)) hydrogel and gum tragacanth cross-linked 2-hydroxyethyl methacrylate-co-acrylamide/zinc oxide (GT-cl-(HEMA-co-AAm)/ZnO) hydrogel composite with better Hg2+ adsorption capability. GT-cl-(HEMA-co-AAm)/ZnO hydrogel composite (154.8 mg g-1) exhibited higher Hg2+ adsorption than GT-cl-(HEMA-co-AAm) hydrogel. To address the performance of GT-cl-(HEMA-co-AAm) hydrogel and GT-cl-(HEMA-co-AAm)/ZnO hydrogel composite, batch adsorption experiments were successfully conducted under different optimised conditions. At last, in-vitro antibacterial activities of Hg2+ loaded GT-cl-(HEMA-co-AAm) and GT-cl-(HEMA-co-AAm)/ZnO were performed in two different well Staphylococcus aureus (gram-positive) and Pseudomonas aeruginosa (gram-negative) bacteria. As a positive control, ampicillin was employed against both types of bacteria. This methodology for the reusability of material has a great ecofriendly impression for minimising secondary waste derived from adsorption and can help design upgraded antibacterial agents.

Energy production from steam gasification processes and parameters that contemplate in biomass gasifier - A review.

The transformation of biomass using steam gasification is a chemical route to facilitate changes in organic or residue supported carbonaceous substances addicted to carbon mono-oxide, hydrogen including carbon-di-oxide, etc. However, to commercialize the method of steam gasification, the hurdles persist during the gasification as well as downstream processing. This article delivers a summary of the different approaches that are described in the previous studies to achieve H2 refinement and adaptation within the gasifier system. These include advanced aspects in the research and development of biomass gasification (alike advancements under the gasification operation). The upshot of diverse operating conditions like steam flow rate, operating temperature, moisture content, gasifier agents, residence time, biomass to air, steam to biomass, equivalence ratio, etc. towards the execution of biomass gasifier. This review accomplishes that the interdependence of several issues must be considered in point to optimise the producer gas.

Identification, characterization and expression profiles of Fusarium udum stress-responsive WRKY transcription factors in Cajanus cajan under the influence of NaCl stress and Pseudomonas fluorescens OKC.

The WRKY gene family has never been identified in pigeonpea (Cajanus cajan). Therefore, objective of the present study was to identify the WRKY gene family in pigeonpea and characterize the Fusarium udum stress-responsive WRKY genes under normal, NaCl-stressed and Pseudomonas fluorescens OKC (a plant growth-promoting bacterial strain) treated conditions. The aim was to characterize the Fusarium udum stress-responsive WRKY genes under some commonly occurring field conditions. We identified 97 genes in the WRKY family of pigeonpea, using computational prediction method. The gene family was then classified into three groups through phylogenetic analysis of the homologous genes from the representative plant species. Among the 97 identified WRKY genes 35 were further classified as pathogen stress responsive genes. Functional validation of the 35 WRKY genes was done through generating transcriptional profiles of the genes from root tissues of pigeonpea plants under the influence of P. fluorescens OKC after 24 h of stress application (biotic: Fusarium udum, abiotic: NaCl). The entire experiment was conducted in two pigeonpea cultivars Asha (resistant to F. udum) and Bahar (susceptible to F. udum) and the results were concluded on the basis of transcriptional regulation of the WRKY genes in both the pigeonpea cultivars. The results revealed that among the 35 tentatively identified biotic stress responsive CcWRKY genes, 26 were highly F. udum responsive, 17 were better NaCl responsive compared to F. udum and 11 were dual responsive to both F. udum and NaCl. Application of OKC was able to enhance transcript accumulation of the individual CcWRKY genes to both the stresses when applied individually but not in combined challenge of the two stresses. The results thus indicated that CcWRKY genes play a vital role in the defense signaling against F. udum and some of the F. udum responsive CcWRKYs (at least 11 in pigeonpea) are also responsive to abiotic stresses such as NaCl. Further, plant beneficial microbes such as P. fluorescens OKC also help pegionpea to defend itself against the two stresses (F. udum and NaCl) through enhanced expression of the stress responsive CcWRKY genes when the stresses are applied individually.

Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen.

Endophytic Trichoderma hamatum UoM 13 isolated from pearl millet roots was evaluated for its efficiency to suppress downy mildew disease. Under laboratory conditions, T. hamatum seed treatment significantly enhanced pearl millet seed germination and seedling vigor. T. hamatum seed treatment resulted in systemic and durable immunity against pearl millet downy mildew disease under greenhouse and field conditions. T. hamatum treated seedlings responded to downy mildew infection with high lignification and callose deposition. Analysis of defense enzymes showed that T. hamatum treatment significantly enhanced the activities of glucanase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase in comparison to untreated control. RT-PCR analysis revealed differentially expressed transcripts of the defense enzymes and PR-proteins in treated, untreated, and checks, wherein PR-1, PR-5, and cell wall defense HRGPs were significantly over expressed in treated seedlings as against their lower expression in controls. T. hamatum treatment significantly stimulated endogenous salicylic acid (SA) levels and significantly upregulated important SA biosynthesis gene isochorismate synthase. The results indicated that T. hamatum UoM13 treatment induces resistance corresponding to significant over expression of endogenous SA, important defense enzymes, PR-proteins, and HRGPs, suggesting that SA biosynthetic pathway is involved in pearl millet for mounting systemic immunity against downy mildew pathogen.