RESUMO
We studied the expression of ARID1A, prostaglandin E2 synthase, and prostaglandin E2 receptor in the endometrium and ovarian, peritoneal, and intestinal endometrioid heterotopies in women with endometriosis of young and middle reproductive age. ARID1A protein is a tumor suppressor, its expression reduced in different types of cancer. Prostaglandin E2 synthase and prostaglandin E2 receptor are involved in the signaling cascade of inflammatory reactions presumably underlying the development of endometriosis. In endometrioid heterotopies, expression of ARID1A was reduced in 1.2-4.0 times, the expression of prostaglandin E2 synthase and prostaglandin E2 receptor was reduced in 2.9-5.2 times These findings suggest that ARID1A, prostaglandin E2 synthase, and prostaglandin E2 receptor can be used as predictors of malignant transformation of endometrioid heterotopies in women with endometriosis.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Dinoprostona/metabolismo , Endometriose/metabolismo , Fatores de Transcrição/metabolismo , Adulto , Transformação Celular Neoplásica/metabolismo , Transformação Celular Neoplásica/patologia , Proteínas de Ligação a DNA/genética , Dinoprostona/genética , Endometriose/genética , Endométrio/metabolismo , Endométrio/patologia , Feminino , Humanos , Fatores de Transcrição/genética , Adulto JovemRESUMO
We compared the expression of Aß42 peptide, τ-protein, and α-synuclein in the substantia nigra and skin fibroblasts of elderly and senile patients with Parkinson's disease and subjects without neuropathology. Expression of markers in the studied tissues was assessed by immunohistochemical and immunocytochemical methods. The expression of Aß42 peptide, τ-protein, and α-synuclein in the substantia nigra of elderly and senile patients with Parkinson's disease was higher by 11-31 times than in subjects without neuropathology. In skin fibroblasts of patients with Parkinson's disease, the expression of Aß42 peptide and α-synuclein was 3-14 times higher than in subjects without neuropathology, and expression of τ-protein did not significantly differ in the studied groups. Thus, immunocytochemical analysis of the expression Aß42 peptide and α-synuclein in skin fibroblasts can be a simple method of early diagnosis of Parkinson's disease in elderly persons.
Assuntos
Fibroblastos/citologia , Doença de Parkinson/diagnóstico , Doença de Parkinson/metabolismo , Pele/citologia , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Peptídeos beta-Amiloides/metabolismo , Biomarcadores/metabolismo , Feminino , Fibroblastos/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , alfa-Sinucleína/metabolismoRESUMO
The expression of cytokine LIF (leukemia inhibiting factor) in the endometrium of women of young reproductive age with non-developing pregnancy of unknown genesis is higher than in older women and women with infection-related miscarriages by 1.26 and 1.43 times, respectively. The expression of angiogenesis marker CD34 in the endometrium of young women with non-developing pregnancy of unknown origin is 1.59 times higher and in case of endometrial inflammation 1.31 times higher than in women of the older reproductive age with the same disease. Correlation between the endometrial expression of LIF and CD34 is detected in women of the younger reproductive age with non-developing pregnancy of unknown etiology. The expression of LIF and CD34 can be used for endometrial function evaluation in women of different age with first trimester non-developing pregnancy.
Assuntos
Aborto Habitual/diagnóstico , Antígenos CD34/genética , Fator Inibidor de Leucemia/genética , Neovascularização Patológica/diagnóstico , Aborto Habitual/genética , Aborto Habitual/metabolismo , Aborto Habitual/patologia , Adulto , Fatores Etários , Antígenos CD34/metabolismo , Biomarcadores/metabolismo , Estudos de Casos e Controles , Implantação do Embrião , Endométrio/irrigação sanguínea , Endométrio/metabolismo , Endométrio/patologia , Feminino , Expressão Gênica , Humanos , Fator Inibidor de Leucemia/metabolismo , Neovascularização Patológica/genética , Neovascularização Patológica/metabolismo , Neovascularização Patológica/patologia , Gravidez , Primeiro Trimestre da Gravidez , PrognósticoRESUMO
We analyzed the effect of dipeptide Glu-Trp and isovaleroyl-Glu-Trp in concentrations of 0.2, 2 and 20 µg/ml and Actovegin preparation on functional activity of human skin fibroblasts. Dipeptides, especially Glu-Trp, produce a stimulating effect on human skin fibroblasts and their effect is equivalent to that of Actovegin. Dipeptides stimulate cell renewal processes by activating synthesis of Ki-67 and reducing expression of caspase-9 and enhance antioxidant function of the cells by stimulating the expression of Hsp-90 and inducible NO-synthase. These findings suggest that dipeptides are promising candidates for preparations stimulating reparative processes.
Assuntos
Dipeptídeos/farmacologia , Fibroblastos/metabolismo , Heme/análogos & derivados , Ácidos Pentanoicos/farmacologia , Pele/metabolismo , Antioxidantes/fisiologia , Caspase 9/biossíntese , Proliferação de Células/efeitos dos fármacos , Fibroblastos/citologia , Proteínas de Choque Térmico HSP90/biossíntese , Heme/farmacologia , Hemiterpenos , Humanos , Antígeno Ki-67/biossíntese , Óxido Nítrico Sintase Tipo II/biossíntese , Pele/citologiaRESUMO
The molecular mechanism of atherosclerosis is based on decreased expression of signal molecules connexin (Cx37 and Cx40), vascular endothelial growth factor (VEGF), and proliferative protein Ki-67, which characterize the processes of cell-cell interactions and proliferation of vascular endotheliocytes. Lys-Glu-Trp peptide in doses of 4 and 40 µg/ml promoted recovery of Cx37, Cx40, and VEGF expression in cultured endotheliocytes from the aorta of patients with atherosclerosis, which attests to vasoprotective effects of this agent.
Assuntos
Aterosclerose/patologia , Comunicação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Endoteliais/fisiologia , Oligopeptídeos/farmacologia , Células Cultivadas , Conexinas/metabolismo , Células Endoteliais/efeitos dos fármacos , Endotélio Vascular/patologia , Humanos , Antígeno Ki-67/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Proteína alfa-5 de Junções Comunicantes , Proteína alfa-4 de Junções ComunicantesRESUMO
We studied the effect of polypeptide complex isolated from calf kidney and short peptides T-31 (AED) and T-35 (EDL) on the expression of signaling molecules, markers of cell renewal (Ki-67, p53), remodeling of the extracellular matrix (MMP-14), and immune response (IL-8) in primary kidney cell cultures during aging. The complex of renal polypeptides and T-31 peptide activate cell renewal processes during aging of the renal epithelium, while gelatinase MMP-14 is the target of T-35 peptide.
Assuntos
Senescência Celular/efeitos dos fármacos , Peptídeos/farmacologia , Animais , Células Cultivadas , Interleucina-8/metabolismo , Antígeno Ki-67/metabolismo , Rim , Metaloproteinase 14 da Matriz/metabolismo , Ratos , Ratos Wistar , Proteína Supressora de Tumor p53/metabolismoRESUMO
The mechanism of geroprotective effect of peptides AED and EDL was studied in ageing renal cell culture. Peptide AED and EDL increase cell proliferation, decreasing expression of marker of aging p16, p21, p53 and increasing expression of SIRT-6 in young and aged renal cell culture. The reduction of SIRT-6 synthesis in cell is one of the causes of cell senescence. On the basis of experimental data models of interaction of peptides with various sites of DNA were constructed. Both peptides form most energetically favorable complexes with d(ATATATATAT)2 sequences in minor groove of DNA. It is shown that interaction of peptides AED and EDL with DNA is the cause of gene expression, encoded marker of ageing in renal cells.
Assuntos
Senescência Celular/efeitos dos fármacos , Rim/efeitos dos fármacos , Oligopeptídeos/farmacologia , Animais , Sítios de Ligação , Biomarcadores/metabolismo , Contagem de Células , Células Cultivadas , Inibidor p16 de Quinase Dependente de Ciclina/genética , Inibidor de Quinase Dependente de Ciclina p21/genética , DNA/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Rim/citologia , Rim/metabolismo , Simulação de Acoplamento Molecular , Cultura Primária de Células , Ratos Wistar , Sirtuínas/genética , Fatores de Tempo , Proteína Supressora de Tumor p53/genéticaRESUMO
The study included 60 patients with chronic Halicobacter gastritis (30 with chronic non-atrophic and 30 with atrophic Halicobacter gastritis (CNAHG and CAHG)). The control group was comprised of 15 practically healthy subjects. The aim of the work was to elucidate the role of Helicobacter infection, disturbances of regeneration, endothelin-1 and melatonin-secreting neuroendocrine cells of gastric antrum in progression of chronic Helicobacter gastritis (CHG). It was shown that CHG is due to H. pylori persistence and that patients with CNAHG undergo grade III microbial contamination while in CAHG patients atrophic changes are accompanied by metaplasia of gastric mucosa (GM) and inflammation of different severity. Patients with CNAHG has an increased number of melatonin-positive gastric cells and enhanced apoptotic activity of GM epitheliocytes. Patients with CAHG experience a reduction of melatonin-positive cells correlated with enhanced apoptotic activity of GM epitheliocytes. The number of endothelin-1 positive cells in patients with CNAHG and CAHG was similar to that in controls. Adequate eradication promoted normalization of the number of gastric endothelin-1 and melatonin-secreting neuroendocrine cells in patients with CHG. The apoptotic index reached the control value within 1 month after eradication in CNAHG patients but remained relatively high in CAHG patients.
Assuntos
Gastrite/patologia , Infecções por Helicobacter/patologia , Helicobacter pylori/patogenicidade , Antro Pilórico/citologia , Adulto , Doença Crônica , Progressão da Doença , Endotelina-1/metabolismo , Gastrite/metabolismo , Gastrite/microbiologia , Gastrite Atrófica/metabolismo , Gastrite Atrófica/microbiologia , Gastrite Atrófica/patologia , Infecções por Helicobacter/metabolismo , Infecções por Helicobacter/microbiologia , Humanos , Pessoa de Meia-Idade , Antro Pilórico/metabolismo , Antro Pilórico/microbiologia , Índice de Gravidade de DoençaRESUMO
Geroprotective peptide T-34 regulates the expression of mRNA for various genes. The development of gastric ulcer is associated with morphological and molecular changes resulting from modulation of the synthesis of antioxidant and anti-inflammatory proteins. Peptide T-34 normalizes the synthesis of these proteins by regulating the expression of the corresponding genes.
Assuntos
Anti-Inflamatórios/administração & dosagem , Mucosa Gástrica/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Oligopeptídeos/administração & dosagem , Úlcera Gástrica/tratamento farmacológico , Animais , Claritromicina/administração & dosagem , Cistamina/administração & dosagem , Mucosa Gástrica/metabolismo , Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP70/metabolismo , Helicobacter pylori/crescimento & desenvolvimento , Masculino , Óxido Nítrico Sintase Tipo I/genética , Óxido Nítrico Sintase Tipo I/metabolismo , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , RNA Mensageiro/biossíntese , Ratos , Ratos Sprague-Dawley , Transdução de Sinais/efeitos dos fármacos , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/genética , Úlcera Gástrica/microbiologia , Fator de Transcrição RelA/genética , Fator de Transcrição RelA/metabolismoRESUMO
Morphological and immunohistochemical investigation of the women's afterbirth with premature rupture of membranes (PROM) and well-time ruptures of placental membranes has been carried out. Inflammatory infiltration of membranes and placental deficiency were higher at patients with PROM. The absence of inflammatory changes in placental tissue during growth of anhydrous gap was demonstrated. The optical density and square of MMP-1 expression were higher and TIMP-1 was lower in membranes of PROM-group than in the control one. No significant changes were found in expression of collagen-6.
Assuntos
Colágeno Tipo VI/biossíntese , Ruptura Prematura de Membranas Fetais , Regulação da Expressão Gênica , Metaloproteinase 1 da Matriz/biossíntese , Placenta , Inibidor Tecidual de Metaloproteinase-1/biossíntese , Feminino , Ruptura Prematura de Membranas Fetais/metabolismo , Ruptura Prematura de Membranas Fetais/patologia , Humanos , Placenta/metabolismo , Placenta/patologia , GravidezRESUMO
Neuroimmunoendocrinology is a new science studying the interplay between major regulatory systems (nervous, endocrine, and immune) mediated through regulatory (signal) molecules. Its applied aspects concern elucidation of the influence of various exogenous factors on the organism manifested as alteration of signal molecule homeostasis. Effects of ethanol on organ systems are considered from the standpoint of neuroimmunoendocrinology.
Assuntos
Sistema Endócrino/efeitos dos fármacos , Etanol/farmacologia , Sistema Imunitário/efeitos dos fármacos , Sistema Nervoso/efeitos dos fármacos , Sistema Endócrino/imunologia , Sistema Endócrino/patologia , Humanos , Sistema Imunitário/imunologia , Sistema Imunitário/patologia , Sistema Nervoso/imunologia , Sistema Nervoso/patologiaRESUMO
The present study included 104 patients with gastric disorders associated with Helicobacter pylori infection. Thirty of them presented with chronic atrophic gastritis (CAG), 30 with gastric ulcer (GU), 20 with adenomatous gastric polyps (AGP), and 24 with gastric cancer (GC). The control group was comprised of 12 practically healthy subjects. We elucidated the role of vascular endothelial growth factor (VEGF) and endocrine cells of gastric mucosa producing glucagon (GC) and pancreatic polypeptide (PPP) in the patients with GAG, GU, AGP and GC prior to and after the surgical intervention and following eradication therapy. It was shown that GAG, AGP, and GC were associated with the persistence of Helicobacter pylori infection and accompanied by hyperplasia of GC and PPP-secreting endocrine cells of gastric mucosa. GU was characterized by hypolasia of VEGF-secreting epithelial cells of the stomach and GC and PPP-secreting endocrine cells of gastric mucosa. The levels of VEGF, GC and PPP that directly or indirectly realize their pathological properties through H. pylori, Bcl-2, and proapoptotic protein BAX proved to be of high prognostic value as regards the evolvement and clinical course of gastric disorders associated with Helicobacter pylori infection. The study demonstrated that adequate eradication therapy in patients with H. pylori-associated diseases of the stomach significantly reduces the number of gastric cells secreting VEGF and has practically no effect on the amount of GC and PPP-producing endocrine cells of gastric mucosa.
Assuntos
Glucagon/metabolismo , Infecções por Helicobacter/metabolismo , Infecções por Helicobacter/patologia , Polipeptídeo Pancreático/metabolismo , Gastropatias/microbiologia , Gastropatias/patologia , Fator A de Crescimento do Endotélio Vascular/metabolismo , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Feminino , Humanos , Masculino , Gastropatias/metabolismoRESUMO
The study included 100 patients with duodenal ulcer (DU), 30 with chronic non-atrophic gastritis (CNAG), 30 with chronic atrophic gastritis (CAG) and 12 practically healthy subjects. All patients with DU and CNAG had morphologically confirmed H. pylori infection. The patients with DU were divided into 2 groups each including age-matched subjects with endoscopically, morphologically, immunohistochemically identical characteristics. Those of group 1 underwent eradication of H. pylori by a traditional 7-day procedure including the use of omeprazole (20 mg BID), claritromycin (500 mg BID) and amoxicillin (1000 mg BID). In group 2 this scheme was supplemented by melatonin (3 mg before bedtime). Patients of group 1 continued to use omeprazole and those in group 2 omeprazole + melatonin till the end of the second month. Healthy subjects and patients with CAG served as controls. All patients underwent FGDS on weeks 2 and 4, immunohistochemical study was conducted to detect endothelin-1 and melatonin-positive cells, apoptotic activity of mucosal epitheliocytes from gastric antrum was determined before and 6 weeks after the onset of therapy. It was shown that introduction of melatonin in the scheme of eradication therapy increases efficacy of H. pylori elimination and accelerates DU cicatrization. Two-month therapy with omeprazole + melatonin more effectively normalizes immunohistochemical parameters (endothelin-1 and melatonin-positive cells) and epitheliocyte apoptotic activity, than omeprazole alone.
Assuntos
Úlcera Duodenal/tratamento farmacológico , Endotelina-1/metabolismo , Células Epiteliais/patologia , Mucosa Gástrica/patologia , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/isolamento & purificação , Melatonina/uso terapêutico , Adulto , Antioxidantes/uso terapêutico , Apoptose , Quimioterapia Combinada , Úlcera Duodenal/metabolismo , Úlcera Duodenal/patologia , Células Epiteliais/metabolismo , Feminino , Mucosa Gástrica/metabolismo , Mucosa Gástrica/microbiologia , Infecções por Helicobacter/metabolismo , Infecções por Helicobacter/patologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
RESEARCH OBJECTIVE: to define a role of structural and functional changes of a colon, expression indicators of epithelial cells of mucous coat of colon that produce serotonin and hromogranin-A at in encephalopathy development at liver cirrhoses. We have examined 146 patients with liver cirrhosis of various classes using the clinical, endoscopic, morphologic and immunohistochemical methods. It is established that with increase of a class of a cirrhosis frequency of revealing of a hepatic encephalopathy, structural changes, degree dysbacteriosis of a colon. Progressing of a hepatic encephalopathy at liver cirrhoses associates with development destructive and atrophic changes of a mucous membrane of a colon and a dysbacteriosis. The increase in the morphometric rates of epithelial cells of mucous coat of colon that produce serotonin and hromogranin-A at liver cirrhoses plays a part in development of a manifesting of a hepatic encephalopathy.
Assuntos
Cromogranina A/metabolismo , Colo , Doenças do Colo , Encefalopatia Hepática , Mucosa Intestinal , Cirrose Hepática , Serotonina/metabolismo , Colo/metabolismo , Colo/patologia , Colo/fisiopatologia , Doenças do Colo/metabolismo , Doenças do Colo/patologia , Doenças do Colo/fisiopatologia , Feminino , Encefalopatia Hepática/metabolismo , Encefalopatia Hepática/patologia , Encefalopatia Hepática/fisiopatologia , Humanos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Mucosa Intestinal/fisiopatologia , Cirrose Hepática/metabolismo , Cirrose Hepática/patologia , Cirrose Hepática/fisiopatologia , Masculino , Pessoa de Meia-IdadeRESUMO
The neuroendocrine mechanisms of development and aging of ovary as the main reproductive organ are considered in the review. There are the analysed data of new investigation which is connected with the role of immune cells of ovary at involutive processes in reproductive system.
Assuntos
Climatério , Hormônios Gonadais/metabolismo , Ovário , Pós-Menopausa , Climatério/imunologia , Climatério/metabolismo , Feminino , Humanos , Imunidade , Longevidade , Sistemas Neurossecretores/metabolismo , Ovário/fisiologia , Ovário/fisiopatologia , Pós-Menopausa/imunologia , Pós-Menopausa/metabolismo , Fenômenos Reprodutivos FisiológicosRESUMO
The expression of matrix metalloproteinase 2 and 9 in thymus and pineal gland has been verified. These data demonstrate single mechanism of remodelling extracellular matrix in thymus and pineal gland at aging.
Assuntos
Matriz Extracelular/metabolismo , Glândula Pineal/metabolismo , Timo/metabolismo , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/metabolismo , Envelhecimento/patologia , Matriz Extracelular/patologia , Humanos , Imuno-Histoquímica , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Pessoa de Meia-Idade , Glândula Pineal/patologia , Timócitos/metabolismo , Timo/patologiaRESUMO
Matrix metalloproteinases (MMP) 2 and 9 has been verified in pineal gland, where MMP take part in remodelling extracellular matrix. Descrease of number MMP2 and 9 has been verified in pineal gland of old age and long liver people in comparison with middle age people. Pinealocytes and/or immune cells of pineal gland can synthesize matrix metalloproteinases.
Assuntos
Envelhecimento/metabolismo , Matriz Extracelular/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Glândula Pineal/metabolismo , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Envelhecimento/patologia , Matriz Extracelular/patologia , Humanos , Imuno-Histoquímica , Linfócitos/metabolismo , Pessoa de Meia-Idade , Glândula Pineal/patologiaRESUMO
The changes in the processes of differentiation, proliferation and apoptosis were studied in the culture of human cortical thymocytes after cell exposure to T-32 and T-38 bioregulator peptides. T-32 and T-38 peptides were shown to enhance the differentiation of immature cortical thymocytes (CD4+CD8+) into T-regulatory cells by increasing their proliferate activity and decreasing the level of apoptosis. Moreover, these peptides were found to stimulate the proliferative and antiapoptotic activity of mature T-regulatory (CD4+CD25+) cells.
Assuntos
Apoptose/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Oligopeptídeos/farmacologia , Linfócitos T Reguladores/imunologia , Timócitos/imunologia , Apoptose/imunologia , Diferenciação Celular/imunologia , Células Cultivadas , Humanos , Linfócitos T Reguladores/citologia , Timócitos/citologiaRESUMO
The use of immunohistochemical method enabled to obtain for the first time expression ferments of extracellular matrix MMP2 and MMP9 in human thymus. Expression of matrix metalloproteinases is discreased in thymus of old age people in comparison with middle age people. These data testify to the important role of metalloproteinases 2 and 9 in extracellular interaction and proliferation activity in thymus aging.
Assuntos
Envelhecimento/fisiologia , Senescência Celular , Metaloproteinase 2 da Matriz/fisiologia , Metaloproteinase 9 da Matriz/fisiologia , Timo/fisiologia , Idoso , Envelhecimento/metabolismo , Feminino , Humanos , Masculino , Metaloproteinase 2 da Matriz/análise , Metaloproteinase 9 da Matriz/análise , Pessoa de Meia-Idade , Timo/citologia , Timo/enzimologiaRESUMO
The review presents the interference between thymus and pineal gland during their involution. The research data of thymus peptides influence on pineal gland and pineal peptides on thymus are summarized. Analysis of these data showed that pineal peptides (Epithalamin, Epitalon) had more effective geroprotective effect on thymus involution in comparison with geroprotective effect of thymic peptides (Thymalin, Thymogen) on involution of pineal gland. The key mechanisms of pineal peptides effect on thymus dystrophy is immunoendocrine cooperation, which is realized as transcription's activation of various proteins.