Unveiling new myrsinoic acids and AChE ligands from Myrsine guianensis (Aubl.) Kuntze.

Molecular dereplication and drug-like discovery are important tools for exploring the chemical profile of metabolites in a complex mixture. In order to establish a workflow for discovering novel acetylcholinesterase (AChE) ligands, we performed the chemical study of Myrsine guianensis (Aubl.) Kuntze (Primulaceae). To carry out the bioprospection, nine extracts were obtained from different parts of the plant. Through the dereplication approaches, seventeen metabolites were annotated. In order to confirm the putative inferences, a HPLC preparative method was developed to isolate three known myrsinoic acids, A(1), B(2) and C(3). Along with, we are reporting the obtention of two new congeners, G(5) and H(6), which their structures were elucidated by NMR and HRMS data. Besides that, two extracts were submitted to affinity assays to accelerate the discovery of AChE ligands. Desorbates were analyzed through LC-HRMS for calculating the affinity ratio (AR). Thus, (1) presented AR = 4.59, therefore was considered a potential ligand.

Experiences of mothers with early weaning: a grounded theory.

OBJECTIVE: To understand the experiences of mothers with early weaning. METHOD: Qualitative research with a theoretical-methodological contribution from Grounded Theory (Straussian perspective), carried out in the context of primary health care in a medium-sized municipality in the northeast of Brazil. 19 collaborators participated by theoretical sampling. Data collection took place between April and September 2018, with in-depth interviews, and was analyzed in three stages: open and axial coding, and integration. RESULTS: The central category "Women experiencing guilt and overload due to early weaning" was supported by three categories: a) conditions: "Showing the factors that limit breastfeeding"; b) actions/interactions: "Trying to balance motherhood and work during breastfeeding" and "Insufficient social support to keep exclusive breastfeeding"; and c) consequences: "Introducing formula and complementary foods before six months" and "Blaming oneself for the early weaning". FINAL CONSIDERATIONS: The theoretical model can give support to managers and health professionals to advocate for longer maternity leaves and confront gender disparities and inequities, professional performance with conflicts of interest, and abusive marketing regarding the use of formula.

Validação de guia prático para profissionais da saúde: adesão ao tratamento da tuberculose / Validation of a practical guide for healthcare professionals: adherence to tuberculosis treatment

Validar um guia prático para os profissionais da APS que oriente a abordagem e contribua no processo de adesão ao tratamento da tuberculose. Trata-se de um estudo metodológico, que buscou validar uma tecnologia educacional por especialistas na temática. Utilizou-se um instrumento para avaliação do material que contou com uma escala tipo Likert. Para análise dos dados, foram calculados os Índices de Validação de Conteúdo (IVC), cuja taxa mínima de concordância adotada foi 0,80. O material foi considerado validado, onde obteve IVC global satisfatório de 0,86. O estudo demonstrou que o material tem potencial para contribuir no manejo de pacientes com tuberculose, uma vez que reúne informações da perspectiva de pessoas que tiveram a doença e alcançaram a cura.

To validate a practical guide for PHC professionals to guide the approach and contribute to the process of adherence to the treatment of tuberculosis. This was a methodological study, which sought to validate an educational technology by specialists in the subject. An instrument was applied to evaluate the material, which used a Likert scale. For data analysis, the Content Validity Index (CVI) was calculated, whose minimum agreement rate adopted was 0.80. The material was considered validated, with a satisfactory global CVI of 0.86. The study demonstrated that the material has the potential to contribute to the management of patients with tuberculosis since it gathers information from the perspective of people who had the disease and were cured.

Mind the gap: The challenges of sustainable forensic science service provision.

Recent worldwide humanitarian and security efforts reflect the growth of forensic science as a global practice supporting peace, prosperity, and justice. While the dominance of the Global North in published research and public attention may suggest that this practice is universally well-developed, many Global South jurisdictions are at a stark disadvantage in resourcing and technological capabilities. Continued development of forensic science as an international endeavour requires a narrowing of inequalities between jurisdictions, in line with the United Nations Sustainable Development Goals. Here we propose a framework of principles for the sustainable provision of transparent, high-quality forensic services meeting jurisdictional needs and limitations. We illustrate how this concept of 'frugal forensics' can be applied in the context of latent fingermark detection in two Global South jurisdictions, and how quality assurance frameworks can be developed to support this service.

Experiences of mothers with early weaning: a grounded theory / Experiencias de madres en el destete temprano: teoría fundamentada em los datos / Vivências de mães no desmame precoce: uma teoria fundamentada nos dados

ABSTRACT Objective: To understand the experiences of mothers with early weaning. Method: Qualitative research with a theoretical-methodological contribution from Grounded Theory (Straussian perspective), carried out in the context of primary health care in a medium-sized municipality in the northeast of Brazil. 19 collaborators participated by theoretical sampling. Data collection took place between April and September 2018, with in-depth interviews, and was analyzed in three stages: open and axial coding, and integration. Results: The central category "Women experiencing guilt and overload due to early weaning" was supported by three categories: a) conditions: "Showing the factors that limit breastfeeding"; b) actions/interactions: "Trying to balance motherhood and work during breastfeeding" and "Insufficient social support to keep exclusive breastfeeding"; and c) consequences: "Introducing formula and complementary foods before six months" and "Blaming oneself for the early weaning". Final considerations: The theoretical model can give support to managers and health professionals to advocate for longer maternity leaves and confront gender disparities and inequities, professional performance with conflicts of interest, and abusive marketing regarding the use of formula.

RESUMEN Objetivo: Comprenderlas experiencias de las madres enel destete precoz. Método: Teoría fundamentada em losdatos. La muestra teórica estuvo compuesta por 19 participantes: madres, familiares y profesionales de la salud de una Unidad Básica de Salud de la región Nordeste de Brasil. La recolección de datos ocurrió de abril a septiembre de 2018, con entrevistas enprofundidad, analizadas a través de codificación abierta, axial y de integración. Resultados: Emergió la categoría central "La mujer experimentando culpa y sobrecarga por el destete precoz", sustentada entres categorías: a) condicionantes: "revelando las limitaciones para lalactancia materna"; b) acciones/interacciones: "intentar conciliar la lactancia materna con la práctica profesional" y "recibir apoyo social insuficiente para mantener la lactancia materna exclusiva"; y c) consecuencias: "introducir fórmulas infantiles y alimentación complementaria antes de los seis meses de edad y culpabilizarse por el destete precoz". Consideraciones finales: Las madres experimentaron la falta de una red de apoyo, la insuficiencia del tiempo de licencia por maternidad y el sentimiento de culpa.

RESUMO Objetivo: Compreender vivências de mães no desmame precoce. Método: Estudo qualitativo orientado pelo referencial metodológico da teoria fundamentada nos dados. A amostragem teórica foi composta por 19 participantes: mães, familiares e profissionais da saúde de uma Unidade Básica de Saúde, todos da Região Nordeste, Brasil. A coleta de dados ocorreu de abril a setembro de 2018, com entrevistas individuais em profundidade, analisadas por meio da codificação aberta, axial e de integração. Resultados: Emergiu o fenômeno "A mulher vivenciando a culpa e a sobrecarga pelo desmame precoce", sustentado por cinco categorias inter-relacionadas. Considerações finais: As mães vivenciaram a carência da rede de apoio, a insuficiência do tempo da licença-maternidade e o sentimento de culpa.

Representações sociais da maternidade para mulheres em privação de liberdade no sistema prisional feminino / Social representations of motherhood for women deprived of liberty in the female prison system

Resumo Neste artigo, objetivou-se analisar as representações sociais da maternidade de mulheres gestantes, lactantes e que vivenciaram a gestação em privação de liberdade no sistema prisional. Trata-se de estudo qualitativo, ancorado nos pressupostos do Paradigma Teórico das Representações Sociais, realizado com 42 mulheres. As participantes eram, em maioria, jovens entre 18 e 39 anos de idade (90,5%; n=38) e se encontravam solteiras (50,0%; n=21); 61,9% (n=26) relataram duas ou mais gestações e 47,6% (n=20) referiram um ou mais abortamentos. A possível representação do ser mãe na prisão se cristalizou, em termos semânticos, principalmente pelos termos "separação" (f=27; OME: 2,9), "tristeza" (f=18; OME: 2,3), "horrível" (f=16; OME: 2,1) e "dor" (f=12; OME: 2,8). Na zona de substituição e de descontextualização, as representações foram objetivadas pelos termos "separação" (f=18; OME: 3), "tristeza" (f=13; OME: 2,5), "medo" (f=11; OME: 2,2) e "horrível" (f=10; OME: 1,5). Evidenciou-se que a centralidade das representações sociais para as participantes do estudo reflete o sofrimento vivenciado pela separação da díade mãe-filho.

Abstract This paper aimed to analyze the social representations of motherhood of pregnant women, breastfeeding women, and those who experienced pregnancy in deprivation of liberty in the prison system. This qualitative study was conducted with 42 women and is grounded on the assumptions of the Theoretical Paradigm of Social Representations. Most participants were young women aged 18-39 (90.5%; n=38) and single (50.0%; n=21); 61.9% (n=26) reported two or more pregnancies and 47.6% (n=20) reported one or more miscarriages. The possible representation of being a mother in prison was crystallized, in semantic terms, mainly by the terms "separation" (f=27; OME: 2.9), "sadness" (f=18; OME: 2.3), "horrible" (f=16; OME: 2.1) and "pain" (f=12; OME: 2.8). In the substitution and decontextualization zone, representations were objectified by the terms "separation" (f=18; OME: 3), "sadness" (f=13; OME: 2.5), "fear" (f=11; OME: 2.2) and "horrible" (f=10; OME: 1.5). It was evident that the centrality of social representations for the study participants reflects the suffering experienced by the separation of the mother-child dyad.

Social representations of motherhood for women deprived of liberty in the female prison system. / Representações sociais da maternidade para mulheres em privação de liberdade no sistema prisional feminino.

This paper aimed to analyze the social representations of motherhood of pregnant women, breastfeeding women, and those who experienced pregnancy in deprivation of liberty in the prison system. This qualitative study was conducted with 42 women and is grounded on the assumptions of the Theoretical Paradigm of Social Representations. Most participants were young women aged 18-39 (90.5%; n=38) and single (50.0%; n=21); 61.9% (n=26) reported two or more pregnancies and 47.6% (n=20) reported one or more miscarriages. The possible representation of being a mother in prison was crystallized, in semantic terms, mainly by the terms "separation" (f=27; OME: 2.9), "sadness" (f=18; OME: 2.3), "horrible" (f=16; OME: 2.1) and "pain" (f=12; OME: 2.8). In the substitution and decontextualization zone, representations were objectified by the terms "separation" (f=18; OME: 3), "sadness" (f=13; OME: 2.5), "fear" (f=11; OME: 2.2) and "horrible" (f=10; OME: 1.5). It was evident that the centrality of social representations for the study participants reflects the suffering experienced by the separation of the mother-child dyad.

Neste artigo, objetivou-se analisar as representações sociais da maternidade de mulheres gestantes, lactantes e que vivenciaram a gestação em privação de liberdade no sistema prisional. Trata-se de estudo qualitativo, ancorado nos pressupostos do Paradigma Teórico das Representações Sociais, realizado com 42 mulheres. As participantes eram, em maioria, jovens entre 18 e 39 anos de idade (90,5%; n=38) e se encontravam solteiras (50,0%; n=21); 61,9% (n=26) relataram duas ou mais gestações e 47,6% (n=20) referiram um ou mais abortamentos. A possível representação do ser mãe na prisão se cristalizou, em termos semânticos, principalmente pelos termos "separação" (f=27; OME: 2,9), "tristeza" (f=18; OME: 2,3), "horrível" (f=16; OME: 2,1) e "dor" (f=12; OME: 2,8). Na zona de substituição e de descontextualização, as representações foram objetivadas pelos termos "separação" (f=18; OME: 3), "tristeza" (f=13; OME: 2,5), "medo" (f=11; OME: 2,2) e "horrível" (f=10; OME: 1,5). Evidenciou-se que a centralidade das representações sociais para as participantes do estudo reflete o sofrimento vivenciado pela separação da díade mãe-filho.

Allergic sensitization and exposure to ambient air pollution beginning early in life lead to a COPD-like phenotype in young adult mice.

The perinatal period and early infancy are considered critical periods for lung development. During this period, adversities such as environmental exposures, allergic sensitization, and asthma are believed to impact lung health in adulthood. Therefore, we hypothesized that concomitant exposure to allergic sensitization and urban-derived fine particulate matter (PM2.5) in the early postnatal period of mice would cause more profound alterations in lung alveolarization and growth and differently modulate lung inflammation and gene expression than either insult alone in adult life. BALB/c mice were sensitized with ovalbumin (OVA) and exposed to PM2.5 from the fifth day of life. Then, we assessed lung responsiveness, inflammation in BALF, lung tissue, and alveolarization by stereology. In addition, we performed a transcriptomic analysis of lung tissue on the 40th day of life. Our results showed that young adult mice submitted to allergic sensitization and exposure to ambient PM2.5 since early life presented decreased lung growth with impaired alveolarization, a mixed neutrophilic-eosinophilic pattern of lung inflammation, increased airway responsiveness, and increased expression of genes linked to neutrophil recruitment when compared to animals that were OVA-sensitized or PM2.5 exposed only. Both, early life allergic sensitization and PM2.5 exposure, induced inflammation and impaired lung growth, but concomitant exposure was associated with worsened inflammation parameters and caused alveolar enlargement. Our experimental data provide pathological support for the hypothesis that allergic or environmental insults in early life have permanent adverse consequences for lung growth. In addition, combined insults were associated with the development of a COPD-like phenotype in young adult mice. Together with our data, current evidence points to the urgent need for healthier environments with fewer childhood disadvantage factors during the critical windows of lung development and growth.

Efficacy of the combination of P5 peptide and photodynamic therapy mediated by bixin and chlorin-e6 against Cutibacterium acnes biofilm.

In this study, the action of antimicrobial peptide (AMP) P5 and antimicrobial photodynamic therapy (aPDT) mediated by bixin and chlorin-e6 (Ce6) on Cutibacterium acnes (C. acnes) in planktonic phase and biofilm were evaluated both as monotherapies and combined therapies. Microbial viability after treatments were quantified by colony-forming units per milliliter of the sample (CFU/mL) and have demonstrated that all treatments employed exerted bactericidal activity, reducing the microbial load by more than 3 log10 CFU/mL, also demonstrating for the first time in the literature the antimicrobial photodynamic effect of bixin that occurs mostly through type I mechanism which was proved by the quantification of superoxide anion production. Bacterial biofilm was completely eliminated only after its exposure to aPDT mediated by this PS, however, Ce6 proved to be a more efficient PS, considering that most of the photodynamic effect of bixin- aPDT was exerted by excitation of the endogenous C porphyrins of C. acnes with blue light. The combination of P5 with Ce6-aPDT showed a synergistic effect on the bacterial biofilm with a reduction in microbial load by more than 10 log10 CFU/mL, in which the ability of P5 to permeabilize the polymeric extracellular matrix of the biofilm explains the obtained results, with greater internalization of the PS as shown by the Confocal Laser Scanning Microscopy. One-way ANOVA (Analysis of Variance) with Tukey's post-test and two-way ANOVA with Bonferroni's post-test were used to compare the values of continuous variables between the control group and the treatment groups.

Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil.

Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of "Triângulo Mineiro" and "Alto Paranaíba" regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.

Cigarette butts in two urban areas from Brazil: Links among environmental impacts, demography and market.

Environmental impacts are currently linked to smoking cigarette behavior, as cigarette butts (CBs) represent the most common litter item in natural areas. Despite this, even the best ranked Brazilian cities, in terms of urban cleaning, have no information about urban littered CBs. Thus, CBs were monitored in Santos and Niterói cities, aiming to assess contamination, Cigarette Butt Pollution Index (CBPI) and the illegal market size. CBs were collected in 36 walkways considering different land usage types and urban density levels. The CBPI was calculated, and brands were used to identify the size of the illegal market. CBs contamination in Santos (0.25 CBs/m2) was three times higher than Niterói (0.08 CBs/m2) and their occurrence and distribution presented no relationship with land usage types and urban population densities levels. CBPI = 17.6 was severe and the highest so far reported. A total of 28 cigarette brands were found both studied cities. Further, illegal cigarette consumption in Santos and Niteroi was estimated, based on brands of collected CBs, at 25.2% and 36.8%, respectively. Such data may be valuable for implementation of logistic reverse actions seeking to environmentally sustainable and socially resilient cities. Cigarette consumption threatens human life and the environment, and tobacco companies should be accountable for the pollution they generate.

Resource-oriented sanitation: Identifying appropriate technologies and environmental gains by coupling Santiago software and life cycle assessment in a Brazilian case study.

Implementation of resource recovery technologies is becoming increasingly important, as humans are exhausting the world's natural resources. Recovering nutrients and water from wastewater treatment systems will play an important role in changing the current trends towards a circular economy. However, guidance is still needed to determine the most appropriate way to do this. In this study two decision-support tools, sanitation planning software (Santiago) and life cycle assessment (LCA), were applied to identify appropriate technologies and their environmental impacts. As a case study, current and alternative scenarios for a wastewater treatment plant (WWTP) in Campo Grande, west-central Brazil, were used. Among 12 scenarios provided by Santiago for efficient nutrient recovery, eight were selected for further assessment. The current WWTP system (UASB reactors) resulted in the highest negative impacts in two of nine assessment categories (freshwater and marine eutrophication), due to nutrient discharge to water. A source separation scenario with urine stored in a urine bank and co-composting of feces showed best overall performance. Electricity consumption played a crucial role for impacts in several categories, while water consumption was not significantly affected by choice of toilet. One Santiago scenario matched the most appropriate scenario with the best environmental performance, but the other seven scenarios were not as beneficial, indicating a need for some adjustments in the software. These results highlight the importance of performing LCA to compare alternative scenarios, even when using a tool designed to identify locally appropriate technologies. The results also indicate that the current wastewater treatment system has reasonable environmental performance, but could be improved if measures were taken to recover energy and reuse water.

Phantom sensation and quality of life among patients with lower-limb amputations in the region of Juiz de Fora, Minas Gerais: a cross-sectional study.

An amputation is an irreversible event that causes social, psychological, and functional consequences that reduces the quality of life of the amputee. Phantom pain generally is reported by 50 to 80% of amputees. OBJECTIVE: To describe the pain and phantom sensation and quality of life among lower-limb amputees. METHODS: This was a cross-sectional study carried out in the region of Juiz de Fora, state of Minas Gerais, Brazil. Inclusion criteria were being a patient in one of two hospitals in the region at the time of the interview and having at least one lower-limb amputation. A total of 20 amputees were included in the analysis. The interview questionnaire had items adapted from the Groningen Questionnaire Problems After Leg Amputation - describing the frequency and discomfort of phantom pain and sensation, causes and the level of the amputation, as well as the WHOQOL-BREF, for assessing quality of life. RESULTS: Most participants were women (55%) and had a mean age of 55.6 years (SD=14.8). Femoral amputation was the most prevalent (65%), and diabetes (40%) was the main reason for amputation. 29% of amputees classified the phantom pain as moderate or severe, and 15% claimed daily frequency of this phenomenon. As for phantom pain, only 6% stated daily frequency. The mean quality of life was 4.1 (SD=1.1, five score means very satisfied), the physical domain of quality of life had the lowest mean (3.4, SD=0.7). CONCLUSIONS: Phantom sensation and pain were prevalent among lower-limb amputees who were, in general, less satisfied with their physical domain of quality of life.

A amputação é um procedimento irreversível que gera consequências sociais, psicológicas e funcionais. A dor do membro fantasma ocorre entre 50 e 80% dos amputados, apresentando implicações na qualidade de vida. OBJETIVO: Avaliar a sensação, dor fantasma e qualidade de vida entre pacientes amputados. MÉTODOS: Trata-se de um estudo transversal retrospectivo realizado na região de Juiz de Fora, Minas Gerais, Brasil. Os critérios de inclusão foram possuir pelo menos uma amputação de membro inferior e ser paciente de um dos hospitais referência para região. Vinte participantes foram incluídos nas análises. O questionário da pesquisa consistiu em itens, adaptados do Groningen Questionnaire Problems After Leg Amputation, que descrevem frequência e incômodo da sensação fantasma, dor fantasma, causas e nível da amputação, e questões sobre os tratamentos além do questionário qualidade de vida WHOQOL-BREF. RESULTADOS: A maioria dos participantes era do sexo feminino (55%) com idade média de 55,6 (DP=14,8) anos. A maior frequência de amputação foi transfemural (65%), e o principal motivo da amputação foi o diabetes mellitus (40%). Com relação ao incômodo da sensação fantasma, 29% a classificou como moderado ou severa, e 15% alegou frequência diária desse fenômeno. Quanto {a dor fantasma, apenas 6% afirmou frequência diária. A qualidade de vida média dos participantes foi de 4,1 (DP=1,1) em 5 (cinco significa muito satisfeito), de acordo com a primeira questão do WHOQOL-BREF; o domínio físico apresentou a menor média 3,4 (DP=0,7). CONCLUSÃO: A sensação e dor fantasmas foram prevalentes nos entrevistados que apresentaram menor satisfação com o domínio físico da qualidade de vida.

Effect of Carvacrol and Thymol on NorA efflux pump inhibition in multidrug-resistant (MDR) Staphylococcus aureus strains.

Undue exposure to antimicrobials has led to the acquisition and development of sophisticated bacterial resistance mechanisms, such as efflux pumps, which are able to expel or reduce the intracellular concentration of various antibiotics, making them ineffective. Therefore, inhibiting this mechanism is a promising way to minimize the phenomenon of resistance in bacteria. In this sense, the present study sought to evaluate the activity of the Carvacrol (CAR) and Thymol (THY) terpenes as possible Efflux Pump Inhibitors (EPIs), by determining the Minimum Inhibitory Concentration (MIC) and the association of these compounds in subinhibitory concentrations with the antibiotic Norfloxacin and with Ethidium Bromide (EtBr) against strains SA-1199 (wild-type) and SA-1199B (overexpresses NorA) of Staphylococcus aureus. In order to verify the interaction of the terpenes with the NorA efflux protein, an in silico molecular modeling study was carried out. The assays used to obtain the MIC of CAR and THY were performed by broth microdilution, while the Efflux Pump inhibitory test was performed by the MIC modification method of the antibiotic Norfloxacin and EtBr. docking was performed using the Molegro Virtual Docker (MVD) program. The results of the study revealed that CAR and THY have moderate bacterial activity and are capable of reducing the MIC of Norfloxacin antibiotic and EtBr in strains of S. aureus carrying the NorA efflux pump. The docking results showed that these terpenes act as possible competitive NorA inhibitors and can be investigated as adjuvants in combined therapies aimed at reducing antibiotic resistance.

Phantom sensation and quality of life among patients with lower-limb amputations in the region of Juiz de Fora, Minas Gerais: a cross-sectional study / Qualidade de vida e sensação fantasma em pacientes amputados em membro inferior da região de Juiz de Fora, Minas Gerais: um estudo transversal

ABSTRACT. An amputation is an irreversible event that causes social, psychological, and functional consequences that reduces the quality of life of the amputee. Phantom pain generally is reported by 50 to 80% of amputees. Objective: To describe the pain and phantom sensation and quality of life among lower-limb amputees. Methods: This was a cross-sectional study carried out in the region of Juiz de Fora, state of Minas Gerais, Brazil. Inclusion criteria were being a patient in one of two hospitals in the region at the time of the interview and having at least one lower-limb amputation. A total of 20 amputees were included in the analysis. The interview questionnaire had items adapted from the Groningen Questionnaire Problems After Leg Amputation - describing the frequency and discomfort of phantom pain and sensation, causes and the level of the amputation, as well as the WHOQOL-BREF, for assessing quality of life. Results: Most participants were women (55%) and had a mean age of 55.6 years (SD=14.8). Femoral amputation was the most prevalent (65%), and diabetes (40%) was the main reason for amputation. 29% of amputees classified the phantom pain as moderate or severe, and 15% claimed daily frequency of this phenomenon. As for phantom pain, only 6% stated daily frequency. The mean quality of life was 4.1 (SD=1.1, five score means very satisfied), the physical domain of quality of life had the lowest mean (3.4, SD=0.7). Conclusions: Phantom sensation and pain were prevalent among lower-limb amputees who were, in general, less satisfied with their physical domain of quality of life.

RESUMO. A amputação é um procedimento irreversível que gera consequências sociais, psicológicas e funcionais. A dor do membro fantasma ocorre entre 50 e 80% dos amputados, apresentando implicações na qualidade de vida. Objetivo: Avaliar a sensação, dor fantasma e qualidade de vida entre pacientes amputados. Métodos: Trata-se de um estudo transversal retrospectivo realizado na região de Juiz de Fora, Minas Gerais, Brasil. Os critérios de inclusão foram possuir pelo menos uma amputação de membro inferior e ser paciente de um dos hospitais referência para região. Vinte participantes foram incluídos nas análises. O questionário da pesquisa consistiu em itens, adaptados do Groningen Questionnaire Problems After Leg Amputation, que descrevem frequência e incômodo da sensação fantasma, dor fantasma, causas e nível da amputação, e questões sobre os tratamentos além do questionário qualidade de vida WHOQOL-BREF. Resultados: A maioria dos participantes era do sexo feminino (55%) com idade média de 55,6 (DP=14,8) anos. A maior frequência de amputação foi transfemural (65%), e o principal motivo da amputação foi o diabetes mellitus (40%). Com relação ao incômodo da sensação fantasma, 29% a classificou como moderado ou severa, e 15% alegou frequência diária desse fenômeno. Quanto {a dor fantasma, apenas 6% afirmou frequência diária. A qualidade de vida média dos participantes foi de 4,1 (DP=1,1) em 5 (cinco significa muito satisfeito), de acordo com a primeira questão do WHOQOL-BREF; o domínio físico apresentou a menor média 3,4 (DP=0,7). Conclusão: A sensação e dor fantasmas foram prevalentes nos entrevistados que apresentaram menor satisfação com o domínio físico da qualidade de vida.

Low complexity wastewater treatment process in developing countries: A LCA approach to evaluate environmental gains.

Reliable Life Cycle Assessment (LCA) indicators for wastewater treatment plants (WWTP) construction and operation phases are still a demand mainly in developing countries. Thus, the purpose of this paper was to present and discuss the environmental performance of a full-scale WWTP installed in a Brazilian city using LCA approach. The treatment process consists of a UASB reactor followed by constructed wetlands, which makes it particularly attractive to developing countries due to its operational simplicity. The Life Cycle Inventory (LCI) was developed from a WWTP design and operation data including those of untreated wastewater and effluent quality. The results show that the environmental impacts from construction phase should not be neglected in LCA studies of low complexity treatment technologies (e.g. UASB reactor, constructed wetlands and pond systems). There is a trade-off between the use of materials and energy for construction and the low energy and materials consumption during the operation phase in these systems. The majority share of hydroelectric generation in the energy matrix and the combination of anaerobic and natural processes for wastewater treatment have contributed to a smaller impact potential for the operation phase. The LCA approach should be associated with plans and actions to face the challenges of providing wastewater treatment in developing countries. Only in this way, compliance with the eco-efficiency targets and protect public health will be guaranteed.

Benzoxazoles as novel herbicidal agents.

BACKGROUND: Despite the need to develop new herbicides with different modes of action, due to weed resistance, many important classes of compounds have been studied poorly for this purpose. Benzoxazoles are considered privileged structures because of their biological activities, but their phytotoxic activities have not received a lot of attention until now. RESULTS: Double vinylic substitution reactions were carried out to furnish four 2-nitromethylbenzoxazoles and one oxazolidine. Benzoxazol-2-ylmethanamine was obtained by reduction of compound 3a. These compounds were evaluated for their phytotoxicity in Allium cepa (onion), Solanum lycopersicum (tomato), Cucumis sativus (cucumber) and Sorghum bicolor (sorghum). Comparison with oxazolidine analogue allowed us to understand that the benzoxazolic structure is very important for the herbicidal activity. CONCLUSION: All the synthesized compounds exhibited biological activity on seed germination. The four 2-nitromethylbenzoxazoles showed phytotoxic activity and the 5-chloro-2-(nitromethyl)benzo[d]oxazole (3b) exhibited higher inhibition than the commercial herbicide against all four plant species tested. © 2018 Society of Chemical Industry.

The presence of the father in the kangaroo method / La presencia del padre en el método canguru / A presença do pai no método canguru

ABSTRACT Objective: To reflect about the father's experience close to the premature son using the kangaroo method under the referential of Merleau-Ponty. Method: Theoretical-reflexive study based on the phenomenology of perception and behavior proposed by Merleau-Ponty and relevant literature. Results: To live the prematurity with the son using the kangaroo method, provides for the father to recognize himself as a being in the world. His intentions regarding the care are demonstrated through the skin to skin contact and the associated care for the newborn child. From the lived experiences, the father can exert the fullness of his paternity. Conclusion and implications for practice: By comprehending the father's way of being, acting and reacting using the kangaroo method, it will be possible to reflect upon his attitudes as caregiver and get him closer to the newborn child. Therefore, it is possible to strengthen the behaviors between father and son with the purpose of creating an emotional bond, thus allowing the inherent care regarding prematurity. Understanding the paternal behavior in the care of the premature child in the Kangaroo Method and their perceptions about care neonatal, can benefit nursing praxis from the perspective of promoting neonatal health, as well as in the prevention of diseases in relation to morbidity and Infant mortality.

RESUMEN Objetivo: Reflexionar sobre la experiencia del padre acerca del hijo prematuro, utilizando el método canguro bajo el referencial de Merleau-Ponty. Método: Estudio teórico-reflexivo basado en la fenomenología de la percepción y el comportamiento propuesto por Merleau-Ponty y la literatura pertinente. Resultados: Convivir con la vida del hijo prematuro, utilizando el método canguro, es favorable para que el padre se reconozca como un ser en el mundo. Sus intenciones con respecto al cuidado se demuestran a través del contacto de piel con piel y del cuidado asociado al niño recién nacido. De las experiencias vividas, el padre puede ejercer la plenitud de su paternidad. Conclusión e implicaciones para la práctica: Al comprender la manera del padre de ser, actuar y reaccionar, usando el método del canguro, será posible reflexionar sobre sus actitudes como cuidador y acercarlo al niño recién nacido. Por lo tanto, es posible fortalecer los comportamientos entre padre e hijo con el propósito de crear un vínculo afectivo, permitiendo así el cuidado inherente con respecto a la prematuridad. La comprensión del comportamiento paternal en el cuidado del niño prematuro en el método canguro y sus percepciones sobre la atención en el período neonatal pueden beneficiar la praxis en enfermería, desde la perspectiva de promover salud neonatal, así como en la prevención de enfermedades en relación a la morbilidad y mortalidad infantil.

RESUMO Objetivo: Refletir sobre a vivência do pai junto ao filho prematuro no Método Canguru sob o referencial de Merleau-Ponty. Método: Estudo teórico-reflexivo baseado na Fenomenologia da Percepção e do Comportamento proposto por Merleau-Ponty e literatura pertinente. Resultados: Vivenciar a prematuridade junto ao filho no Método Canguru propicia ao pai se reconhecer como ser-no-mundo. Suas intenções acerca do cuidado são demonstradas pelo contato pele a pele e do cuidado inerente ao recém-nascido prematuro. A partir das experiências vividas, possibilita ao pai exercer sua paternidade de forma plena e significativa. Conclusão e Implicações para a prática: Compreender as formas de ser, agir e reagir do pai no Método Canguru, possibilita a reflexão de suas atitudes como cuidador e aproxima-o do recém-nascido prematuro. Dessa forma, é possível edificar os comportamentos entre pai e filho no intuito de vinculá-los afetivamente e permitir os cuidados inerentes à prematuridade. Compreender o comportamento paterno nos cuidados ao filho prematuro no Método Canguru e suas percepções acerca do cuidado no período neonatal, pode beneficiar a práxis de enfermagem na perspectiva de promoção à saúde neonatal, bem como na prevenção de agravos no que tange à morbidade e mortalidade infantil.

Vivência de pais com o Método Canguru: revisão integrativa / The experience of fathers with the kangaroo mother care method: an integrative review

Objetivo: compreender a vivência de pais com o Método Canguru. Métodos: revisão integrativa da literatura, realizada nas bases de dados: Scientific Electronic Library Online, Medical Literature Analysis and Retrieval System Online, Literatura Latino-Americana e do Caribe em Ciências da Saúde, Web of Science e Current Nursing and Allied Health Literature. Resultados: obtiveram-se 732 artigos e sete foram selecionados. Identificou-se que pais vivenciam o Método Canguru com realização e prazer. Ademais, apresentaram dificuldades, medo e insegurança que podem estar associados ao funcionamento e a dispositivos de apoio à vida, utilizados para garantir a sobrevida de prematuros no cuidado relacionado ao distanciamento da figura paterna com neonato. Conclusão: a figura paterna, atualmente, apresenta-se mais participativa e consciente acerca do papel no âmbito familiar e nos cuidados inerentes ao filho prematuro. No entanto, pais se sentem despreparados, mas orgulhosos e esperançosos com evolução clínica de bebês, ao vivenciar o Método Canguru. (AU)

Teses e dissertações da enfermagem brasileira sobre saúde da mulher no sistema prisional / Theses and dissertations of Brazilian nursing addressing women's health in the prison system

Objetivo: caracterizar a produção brasileira de teses e dissertações em enfermagem sobre a saúde de mulheres no sistema prisional. Métodos: estudo bibliométrico, a partir do panorama da produção científica, no âmbito da Pós-Graduação em Enfermagem brasileira, com coleta de dados no Catálogo de Teses e Dissertações da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior. Resultados: 11 documentos fizeram parte da amostra. A maioria destes foi realizada durante mestrado (63,6%), com predominância nas Regiões Nordeste (63,6%), Sudeste (18,1%) e Centro-oeste (18,1%). Quanto às temáticas mais exploradas, as relações de mulheres/mães com os filhos apresentaram maior proporção (54,5%). Predominaram os estudos qualitativos (45,4%), com desenhos descritivos (36,3%). Conclusão: teses e dissertações brasileiras de enfermagem sobre a saúde da mulher no sistema prisional constituíram-se por estudos qualitativos, provenientes das regiões Nordeste, Sudeste e Centro-oeste, as quais apresentaram lacuna na produção do conhecimento de enfermagem. (AU)