What we see is what we touch? Sex estimation on the skull in virtual anthropology.

BACKGROUND: The increased use of virtual bone images in forensic anthropology requires a comprehensive study on the observational errors between dry bones and CT reconstructions. Here, we focus on the consistency of nonmetric sex estimation traits on the human skull. MATERIALS AND METHODS: We scored nine nonmetric traits on dry crania and mandibles (n = 223) of archaeological origin and their CT reconstructions. Additionally, we 3D surface scanned a subsample (n = 50) and repeated our observations. Due to the intricate anatomy of the mental eminence, we split it into two separate traits: the bilateral mental tubercles and the midsagittal mental protuberance. We provide illustrations and descriptions for both these traits. RESULTS: We obtained supreme consistency values between the CT and 3D surface modalities. The most consistent cranial traits were the glabella and the supraorbital margin, followed by the nuchal crest, zygomatic extension, mental tubercles, mental protuberance, mental eminence, mastoid process and ramus flexure, in descending order. The mental tubercles show higher consistency scores than the mental eminence and the mental protuberance. DISCUSSION: The increased interchangeability of the virtual modalities with each other as compared to the dry bone modality could be due to the lack of tactility on both the CT and surface scans. Moreover, tactility appears less essential with experience than a precise trait description. Future studies could revolve around the most consistent cranial traits, combining them with pelvic traits from a previous study, to test for accuracy.

Evaluation of porcine decomposition and total body score (TBS) in a central European temperate forest.

The total body score (TBS) is a visual scoring method to scale the succession of decomposition stages. It compares decomposition between cadavers, to connect it with external taphonomic factors and estimate the post-mortem interval. To study decomposition in various climatic environments, pigs are often used as human proxies. Currently, there is one TBS system by Keough et al. (J Forensic Sci. 2017;62:986) for surface-deposited domestic pigs, coming from South Africa. Our study aims to evaluate this method and analyze porcine decomposition in Central Europe to inform forensic research and casework. We conducted an experiment studying six 50 kg pig carcasses in a temperate Swiss forest. Three observers documented decomposition patterns and rated the decomposition stages from photographs based on the porcine TBS model by Keough et al. (J Forensic Sci. 2017;62:986). We documented discrepancies between the carcass decomposition of our specimens and those in the South African study, especially related to the high insect activity in our experiment. Furthermore, we noted factors complicating TBS scoring, including rainfall and scavengers. The agreement between TBS observers from photographs was in the highest agreement category apart from one "substantial agreement" category. Our study is the first in Europe to systematically test the Keough et al. (J Forensic Sci. 2017;62:986) method. The results evidence that regional adaptations are required to be applicable for other environments. We present a modified approach based on experimental observations in a Swiss temperate forest. The identification of regional decomposition patterns and drivers will inform future taphonomy research as well as forensic casework in comparable contexts in Central Europe.

"Until death do us part". A multidisciplinary study on human- Animal co- burials from the Late Iron Age necropolis of Seminario Vescovile in Verona (Northern Italy, 3rd-1st c. BCE).

Animal remains are a common find in prehistoric and protohistoric funerary contexts. While taphonomic and osteological data provide insights about the proximate (depositional) factors responsible for these findings, the ultimate cultural causes leading to this observed mortuary behavior are obscured by the opacity of the archaeological record and the lack of written sources. Here, we apply an interdisciplinary suite of analytical approaches (zooarchaeological, anthropological, archaeological, paleogenetic, and isotopic) to explore the funerary deposition of animal remains and the nature of joint human-animal burials at Seminario Vescovile (Verona, Northern Italy 3rd-1st c. BCE). This context, culturally attributed to the Cenomane culture, features 161 inhumations, of which only 16 included animal remains in the form of full skeletons, isolated skeletal parts, or food offerings. Of these, four are of particular interest as they contain either horses (Equus caballus) or dogs (Canis lupus familiaris)-animals that did not play a dietary role. Analyses show no demographic, dietary, funerary similarities, or genetic relatedness between individuals buried with animals. Isotopic data from two analyzed dogs suggest differing management strategies for these animals, possibly linked to economic and/or ritual factors. Overall, our results point to the unsuitability of simple, straightforward explanations for the observed funerary variability. At the same time, they connect the evidence from Seminario Vescovile with documented Transalpine cultural traditions possibly influenced by local and Roman customs.

Cat and dog scavenging at indoor forensic scenes: strategies for documentation and detection.

Vertebrate scavenging on human remains is occasionally observed at indoor forensic scenes, especially when pets have access to the body and their deceased owners were socially distanced. Pets feeding on corpses have implications for the forensic investigation, e.g. for trauma analysis and the assessment of the cause of death, the estimation of the postmortem interval (PMI), or the recovery of the complete set of remains. Documentation of potential scavenging in forensic practice is tenuous and needs to be improved in order to be able to use the information for future casework. Investigators need to be aware of the alterations pets can cause to human remains and how these affect further analyses. Following a combined literature review for cat and canine scavenging, we present seven new cases from Switzerland with cat and/or dog involvement. We then created a flowchart guide for a systematic collection of data to use at indoor forensic scenes of suspected scavenging. Our literature review revealed the challenge in discriminating between scavenging by domestic cats and dogs, based on the appearance of the lesions alone. Furthermore, the information that is often routinely collected in indoor fatalities with potential scavenging activity is not sufficient to perform this separation. To provide a practical basis for cat and canine scavenging and its differentiation, we summarise strategies and present a flowchart to use in forensic casework of suspected indoor scavenging.

Forensic experiments on animal scavenging: A systematic literature review on what we have and what we need.

Vertebrate scavengers frequently affect forensic casework by feeding on human remains or by scattering body parts and bones. Therefore, animal activity can influence complete recovery of bodies, trauma analysis, and the estimation of the postmortem interval (PMI), potentially hampering identification of the deceased and elucidation of the perimortem circumstances. Experimental research is well suited to investigate scavengers and their impact on carcasses over time, generating knowledge on the forensic relevance of certain scavenger species or communities. However, there are currently no systematised standards to conduct these investigations with a forensic focus, impeding comparison and synthesis of the studies. In our work, we performed a systematic literature review and found 79 publications featuring terrestrial experiments on vertebrate scavenging and/or scattering within a forensic context. We extracted 21 variables describing the study environment, experimental design and the specimens. The results show that there is considerable inconsistency in the study designs and that some of the variables are insufficiently reported. We point out research questions and areas that require attention in future studies, stressing the importance of infrequently mentioned or applied variables. Furthermore, we recommend guidelines to include and report a list of variables in forensic scavenging and scattering experiments. These guidelines will help standardising future research in the field, facilitating inter-study consolidation of results and conclusions, and consequently, inform forensic casework.

The Late Iron Age in Switzerland: a review of anthropological, funerary, and isotopic studies.

The Iron Age in continental Europe is a period of profound cultural and biological importance with heterogeneous trends through space and time. Regional overviews are therefore useful for better understanding the main cultural and biological patterns characterizing this period across the European regions. For the area of modern Switzerland, a rich archeological and anthropological record represents the Late Iron Age. However, no review of the main anthropological and funerary patterns for this period is available to date. Here we assess the available demographic, paleopathological, funerary, and isotopic data for the Late Iron Age in the Swiss territory, and summarize the cultural and biological patterns emerging from the available literature. Finally, we highlight a series of research avenues for future studies.

What we see is what we touch? Sex estimation on the pelvis in virtual anthropology.

BACKGROUND: Computed tomography (CT) scans are a convenient means to study 3D reconstructions of bones. However, errors associated with the different nature of the observation, e.g. visual and tactile (on dry bone) versus visual only (on a screen) have not been thoroughly investigated. MATERIALS AND METHODS: We quantified the errors between modalities for sex estimation protocols of nonmetric (categorical and ordinal) and metric data, using 200 dry pelves of archaeological origin and the CT reconstructions of the same bones. In addition, we 3D surface scanned a subsample of 39 pelves to compare observations with dry bone and CT data. We did not focus on the sex estimation accuracy but solely on the consistency of the scoring, hence, the interchangeability of the modalities. RESULTS: Metric data yielded the most consistent results. Among the nonmetric protocols, ordinal data performed better than categorical data. We applied a slightly modified description for the trait with the highest errors and grouped the traits according to consistency and availability in good, intermediate, and poor. DISCUSSION: The investigated modalities were interchangeable as long as the trait definition was not arbitrary. Dry bone (gold standard) performed well, and CT and 3D surface scans performed better. We recommend researchers test their affinity for using virtual modalities. Future studies could use our consistency analysis and combine the best traits, validating their accuracy on various modalities.

Between Raetia Secunda and the dutchy of Bavaria: Exploring patterns of human movement and diet.

During the transition from Late Antiquity to the Middle Ages, the Roman Empire dissolved in the West and medieval empires were founded. There has been much discussion about the role that migration played in this transition. This is especially true for the formation of the Baiuvariian tribe and the founding of this tribal dukedom, which took place from the 5th to the 6th century in what is now Southern Bavaria (Germany). In this study, we aimed to determine the extent of immigration during the beginning of this transformation and to shed further light on its character. To achieve this goal, we analyzed stable isotope values of strontium, carbon, and nitrogen from the teeth and bones of over 150 human remains from Southern Germany, dating from around 500 AD. This group of individuals included women with cranial modifications (ACD) which can be found sporadically in the burial grounds of this period. Our results showed an above-average migration rate for both men and women in the second half of the 5th century. They also indicate that a foreign background may also be assumed for the women with ACD. The demonstrably different origins of the immigrants from isotopically diverse regions, and the identification of local differences in detectable migration rate, as well as indication for different timing of residential changes, highlight the complexity of immigration processes and the need for more studies at the regional level.

Perimortem Skeletal Sharp Force Trauma: Detection Reliability on CT Data, Demographics and Anatomical Patterns from a Forensic Dataset.

The increasing importance of trauma analysis by means of postmortem computed tomography (PMCT) is insufficiently reflected in forensic curricula, nor are best practice manuals available. We attempt to detect sharp force bone lesions on PMCT of closed forensic cases with the aims of assessing errors and pointing out patterns in anatomical location and manner of death (MOD). We investigated 41 closed sharp force fatality cases, with available PMCT and forensic reports. Two observers with different radiological training assessed the lesions on PMCT scans (2D and 3D) for comparison with the reports. Between 3% (suicides) and 15.3% (homicides) of sharp force injuries caused visible bone lesions. While our observations were repeatable, each forensic investigation left a similar number of bone lesions undetected. Injury patterns differed between MOD, with thoracic bone lesions being most frequent overall. Soft tissue injury location varied between the MOD. Associations between MOD and age as well as number of injuries were significant. The detection of bone lesions on PMCT for untrained forensic specialists is challenging, curricula and pertinent manuals are desirable. With the low frequency of bone lesions compared to soft tissue injuries, we should be aware when analyzing decomposed bodies.

Uncovering Forensic Taphonomic Agents: Animal Scavenging in the European Context.

Animal scavenging by vertebrates can significantly alter human bodies and their deposition site. For instance, vertebrate animals can cause postmortem modification to a body, alter perimortem trauma, influence decomposition rates, disarticulate and scatter body parts or evidence, and affect the identification of the deceased. Animal scavenging is a relatively common occurrence in forensic investigations. Even so, studies on the subject are scattered and rare, with most focussing on geographical areas outside of Europe. For that reason, we intend to collate the literature to provide an account of forensically relevant vertebrate scavengers in Europe, their impacts on human remains, and their implications for forensic investigations. Here, we provide an overview of forensic aspects where the knowledge of animal scavenging is crucial, as well as an account of potential scavengers of human remains in Europe and their typical alterations to soft tissue and, in particular, to bones. In addition, we are the first to provide a guide for forensic practitioners to identify the presence of vertebrate scavenging and subsequently inform outdoor search strategies for affected human remains.

The Kokel of Southern Siberia: New data on a post-Xiongnu material culture.

From the end of the Xiongnu Empire to the establishment of the first Turkic Khaganate, the territory of Southern Siberia sees the emergence of distinctive local material cultures. The Kokel culture is essentially unknown in the international English-language literature even though archaeological sites pertaining to this material culture are among the most common in Tuva (Southern Siberia). This makes them important for the understanding aspects of the sociocultural dynamics following the collapse of the first "steppe empire". Here we present the results of the study of a Kokel funerary site recently excavated near the Early Iron Age kurgan Tunnug 1 and discuss the data in the context of the available Soviet and Russian literature. The Kokel culture substantially differs from the material culture of the Xiongnu and has to be seen as a largely independent cultural entity of small tribal groups without a pronounced social hierarchy engaging in frequent violent local conflict.

Beyond simple kinship and identification: aDNA analyses from a 17th-19th century crypt in Germany.

Ancient DNA (aDNA) analysis is a powerful tool in multidisciplinary research on human remains, potentially leading to kinship scenarios and historical identifications. In this study, we present a genetic investigation of three noble families from the 17th to 19th centuries AD entombed in burial crypts at the cloister church of Riesa (Germany). Tests were aimed at identifying anticipated and incidental genetic relationships in our sample and the implications thereof for the assumed identity of the deceased. A total of 17 individuals were investigated via morphological, radiographic and aDNA analysis, yielding complete and partial autosomal and Y-STR profiles and reliable mtDNA sequences. Biostatistics and lineage markers revealed the presence of first to third degree relationships within the cohort. The pedigrees of the families Hanisch/von Odeleben and von Welck were thereby successfully reproduced, while four previously unknown individuals could be linked to the von Felgenhauer family. However, limitations of biostatistical kinship analysis became evident when the kinship scenario went beyond simple relationships. A combined analysis with archaeological data and historical records resulted in (almost) unambiguous identification of 14 of the 17 individuals.

Co-occurrence of malignant neoplasm and Hyperostosis Frontalis Interna in an Iron Age individual from Münsingen-Rain (Switzerland): A multi-diagnostic study.

OBJECTIVE: To re-analyze one of the oldest cases of malignant bone neoplasm with different analytical techniques. MATERIAL: The available skeletal remains of grave 138 (G138) from the Iron Age necropolis of Münsingen-Rain (Switzerland, 420-240 BC). METHODS: The bones are analyzed by means of morphological, radiographic, histological, and biogeochemical methods. RESULTS: The individual, a male aged between 35-50 years old, presents morphologically and radiographically a previously described coral-like bone neoformation on the proximal left humerus. The new analyses highlight previously undocumented coarse bone proliferation on the left scapula and lobular apposition on the endocranial surface of the frontal bone. The histological analysis of the humerus shows a 'lace-like' pattern of osteoid deposition without lamellation. CONCLUSIONS: Our data support a diagnosis of osteoblastic malignant neoplasm, probably an osteosarcoma or, more likely, a dedifferentiated chondrosarcoma for the humerus and scapula, and of hyperostosis frontalis interna on the frontal. The co-presence of a malignant neoplasm and hyperostosis frontalis interna may be related to a hormonal imbalance, a possibility also suggested by atypical funerary treatment. SIGNIFICANCE: This study confirms G138 as one of the oldest cases of malignant bone neoplasm, adds new paleopathological data on this individual, and demonstrates the advantages of a multidisciplinary approach. LIMITATIONS: The discussion of the pathological changes is limited by the representation and preservation of the skeletal elements. SUGGESTION FOR FUTURE RESEARCH: Biomolecular and protein biomarkers analyses may help to refine the diagnostic conclusions.

Troubles in Tuva: Patterns of perimortem trauma in a nomadic community from Southern Siberia (second to fourth c. CE).

OBJECTIVES: Warfare is assumed to be one of the defining cultural characteristics of steppe nomads in Eastern Eurasia. For the first-centuries CE, a period of political turmoil in Northern China and Southern Siberia, relatively few data are, however, available about the degree and variability of violence in these communities. Here, we provide new data on violence among steppe nomads during the first-centuries CE by analyzing the type, anatomical distribution, and demographic distribution of perimortem trauma at Tunnug1 (Tuva, Southern Siberia-second to fourth c. CE). MATERIALS AND METHODS: Perimortem traumas were assessed on 87 individuals representing both sexes and different age classes. The timing of the lesions was assessed based on morphological criteria, including the absence and presence of bone reactive processes and the relative plasticity of the bone at the moment of impact. The distribution by age, sex, and anatomical location of trauma was analyzed by means of logistic models, Fisher's exact tests, and 3D visualizations. RESULTS: A total of 130 perimortem traumas, including chop marks, slice marks, penetrating lesions, and blunt traumas were identified on 22 individuals. Chop marks were mostly at the level of the skull and vertebrae and were likely caused by bladed weapons. Slice marks were found on the cervical vertebrae and cranium and may be the result of throat slitting and scalping by means of smaller bladed implements. Traumas were more frequent in males, and their presence is not correlated with age. DISCUSSION: This study adds new data to the few available regarding violence among steppe nomadic cultures and provides new insights about the effects of political instability on the life of the people inhabiting Eastern Eurasia during the early centuries CE.

Author Correction: Ancient genomes reveal social and genetic structure of Late Neolithic Switzerland.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Between belief and fear - Reinterpreting prone burials during the Middle Ages and early modern period in German-speaking Europe.

Prone burials are among the most distinctive deviant burials during the Middle Ages and early modern period. Despite their worldwide distribution, the meaning of this burial practice is still a matter of debate. So far, a comprehensive analysis of prone burials is lacking for Central Europe. By compiling evidence from Germany, Switzerland and Austria, this study investigates how these findings fit into the scope of medieval funerary practices. 95 prone burials from 60 archaeological sites were analyzed regarding geographical distribution, dating, burial features, body position, age-at-death and sex. We applied descriptive statistics accompanied by multiple correspondence analysis in order to highlight possible multivariate patterns in the dataset. Prone burials occur in funerary and non-funerary contexts, with a predominance of single churchyard burials, followed by favored and exterior location and settlements. In terms of grave features, the majority of churchyard burials do not differ from regular graves. Multivariate patterns appear to reflect diachronic changes in normative burial practices. We found a significant correlation between burial location and dating, due to a higher frequency of high medieval males in favored locations. In these cases, prone position is interpreted as a sign of humility, while similar evidences from late and post-medieval times are seen as an expression of deviancy. Apparent lack of care during burial reveals disrespect and possible social exclusion, with inhumations outside consecrated ground being the ultimate punishment. In some regions, apotropaic practices suggest that corpses should be prevented from returning, as attested in contemporaneous sources and folk beliefs. We hypothesize that the increase of prone burials towards the late and post-medieval period is linked to such practices triggered by epidemic diseases. The multiplicity of meanings that prone position might have in different contexts demands for careful interpretations within the same regional and chronological frame.

Ancient genomes reveal social and genetic structure of Late Neolithic Switzerland.

Genetic studies of Neolithic and Bronze Age skeletons from Europe have provided evidence for strong population genetic changes at the beginning and the end of the Neolithic period. To further understand the implications of these in Southern Central Europe, we analyze 96 ancient genomes from Switzerland, Southern Germany, and the Alsace region in France, covering the Middle/Late Neolithic to Early Bronze Age. Similar to previously described genetic changes in other parts of Europe from the early 3rd millennium BCE, we detect an arrival of ancestry related to Late Neolithic pastoralists from the Pontic-Caspian steppe in Switzerland as early as 2860-2460 calBCE. Our analyses suggest that this genetic turnover was a complex process lasting almost 1000 years and involved highly genetically structured populations in this region.

Emergence of human-adapted Salmonella enterica is linked to the Neolithization process.

It has been hypothesized that the Neolithic transition towards an agricultural and pastoralist economy facilitated the emergence of human-adapted pathogens. Here, we recovered eight Salmonella enterica subsp. enterica genomes from human skeletons of transitional foragers, pastoralists and agropastoralists in western Eurasia that were up to 6,500 yr old. Despite the high genetic diversity of S. enterica, all ancient bacterial genomes clustered in a single previously uncharacterized branch that contains S. enterica adapted to multiple mammalian species. All ancient bacterial genomes from prehistoric (agro-)pastoralists fall within a part of this branch that also includes the human-specific S. enterica Paratyphi C, illustrating the evolution of a human pathogen over a period of 5,000 yr. Bacterial genomic comparisons suggest that the earlier ancient strains were not host specific, differed in pathogenic potential and experienced convergent pseudogenization that accompanied their downstream host adaptation. These observations support the concept that the emergence of human-adapted S. enterica is linked to human cultural transformations.

Phylogeography of the second plague pandemic revealed through analysis of historical Yersinia pestis genomes.

The second plague pandemic, caused by Yersinia pestis, devastated Europe and the nearby regions between the 14th and 18th centuries AD. Here we analyse human remains from ten European archaeological sites spanning this period and reconstruct 34 ancient Y. pestis genomes. Our data support an initial entry of the bacterium through eastern Europe, the absence of genetic diversity during the Black Death, and low within-outbreak diversity thereafter. Analysis of post-Black Death genomes shows the diversification of a Y. pestis lineage into multiple genetically distinct clades that may have given rise to more than one disease reservoir in, or close to, Europe. In addition, we show the loss of a genomic region that includes virulence-related genes in strains associated with late stages of the pandemic. The deletion was also identified in genomes connected with the first plague pandemic (541-750 AD), suggesting a comparable evolutionary trajectory of Y. pestis during both events.

Those who died very young-Inferences from δ15 N and δ13 C in bone collagen and the absence of a neonatal line in enamel related to the possible onset of breastfeeding.

OBJECTIVES: Stable isotope analysis has often been used in neonatal remains from archeological contexts to investigate the presence of a signal of breastfeeding and weaning in past populations. Tooth histology on the other hand might be used as an indicator of birth survival. This pilot study aimed to investigate the feasibility of using stable nitrogen (δ15 N) and carbon (δ13 C) isotope values from neonatal bone collagen to elucidate if values deviating from the adult female average could indicate breastfeeding and co-occur with the presence of a neonatal line (NNL). The combination of these independent indicators might be useful in clarifying the fate of individuals who died around birth. MATERIALS AND METHODS: Bone collagen from 21 archeological human and animal specimens was extracted and analyzed via mass-spectrometry for δ15 N and δ13 C. A verification of the stable isotope results was undertaken using tooth histology on three individuals who were investigated for the presence of a NNL as an indicator of live birth and short survival. RESULTS: The biological age of the human samples varied between 8.5 lunar months (Lm) and 2 postnatal months (Pm) of age. All except one individual exhibited elevated δ15 N values compared to the female average. The histological analyses revealed no NNL for this and two further individuals (n = 3). DISCUSSION: The results indicate that elevated nitrogen values of very young infants relative to a female average in archeological contexts are not necessarily associated with a breastfeeding onset signal, and therefore cannot be used exclusively as a proxy of birth survival. The elevation might be possible due to various reasons; one could be nutritional, in particular maternal stress during pregnancy or a metabolic disorder of mother and/or her child. In those cases, the evaluation of a NNL might reveal a false breastfeeding signal as seen for two individuals in our sample who have elevated nitrogen values despite the fact no NNL could be observed. Overall, our data support the growing awareness that bone collagen δ15 N values of neonates/infants should not be used as a proxy for breastfeeding or birth survival on its own.