Calcium-phosphate metabolism in patients with multiple sclerosis.

INTRODUCTION AND OBJECTIVES: The purpose of this study was to evaluate the concentration of 25-hydroxycholecalciferol and parameters of calcium-phosphate metabolism at different periods of relapsing-remitting multiple sclerosis (RRMS). MATERIALS AND METHODS: Forty-five patients, residents of Poland (49°-50°, N), were enrolled in the study, i.e. 15 immediately after the diagnosis of RRMS, 15 at the early stage and 15 at the advanced stage of RRMS. The results were compared to values obtained in 20 age- and sex-matched controls. RESULTS: Lower serum concentrations of 25-hydroxycholecalciferol and ionised calcium were found in patients compared to the control group. In patients with the disease duration of 5-6 years, concentrations of 25-hydroxycholecalciferol and ionised calcium were lower than in patients in the earlier period of RRMS. The inverse and clearer direction of changes was found in parathormone serum concentration in patients compared to the controls. In patients with a longer disease duration, a significantly lower 25-hydroxycholecalciferol concentration was found in female patients compared to male patients. In patients, more frequent 25-hydroxycholecalciferol and unsaturated fatty acids' supplementation was observed compared to the controls. CONCLUSIONS: In RRMS patients, calcium-phosphate metabolism is disturbed which increases during disease progression.

Resistance to acetylsalicylic acid in patients with type 2 diabetes mellitus is associated with lipid disorders and history of current smoking.

BACKGROUND: Diabetes mellitus (DM) is an important risk factor for stroke. Acetylsalicylic acid (ASA) is the most frequently used medication for prevention of cardio-cerebral vascular diseases. However, some patients experience ischaemic vascular events despite the use of ASA. This phenomenon is known as "aspirin resistance" (AR). The aim of this study was to assess the prevalence of AR in diabetic patients and search for factors associated with it. MATERIALS AND METHODS: The examined group consisted of 96 subjects with diagnosed type 2 DM. Platelet function test was performed by the method of whole blood impedance aggregometry. RESULTS: Among examined subjects, 51 patients (53.1 %) were sensitive to ASA action (ASA responders) and 45 patients (46.9 %) were resistant to ASA action (ASA non-responders). No association was found between platelet aggregation and gender, age, dose of ASA, known duration of diabetes, BMI, heart rate, mean systolic and diastolic blood pressure, and risk factors except for current smoking (p = 0.030). ASA non-responders were treated shorter with ASA than ASA responders (p = 0.010). The mean total cholesterol (p = 0.020), LDL concentration (p = 0.005), HCT (p = 0.010), WBC (p = 0.030), and PLT (p = 0.050) were significantly higher in ASA non-responders. No association was found between AR and results of other laboratory tests and medications. Multiple logistic regression analysis revealed factors associated with AR: current smoking and LDL concentration higher than 3.5 mmol/l. CONCLUSIONS: Results of our study did not confirm the association between poor glycaemic control in the diabetic patients and AR. Resistance to ASA in diabetic patients is associated with lipid disorders and history of current smoking.

Antioxidative enzymes activity and malondialdehyde concentration during mitoxantrone therapy in multiple sclerosis patients.

Mitoxantrone (MX) is approved for the treatment of aggressive relapsing-remitting, secondary-progressive and progressive-relapsing form of multiple sclerosis (MS). The mechanism of its action is multiaxial, however, it is not free from side effects. The causes of the side effects are still unknown and require further investigation. The aim of this study was to investigate the influence of MX therapy on enzymatic parameters of endogenous antioxidative status: manganese and copper/zinc superoxide dismutase (MnSOD, Cu/ZnSOD), catalase (CAT), glutathione peroxidase (GSH-Px) and lipid peroxidation marker--malondialdehyde (MDA) in blood serum and cerebrospinal fluid (CSF) in patients suffering from MS. After the MX therapy serum and the CSF MDA concentrations increased significantly. We reported that MnSOD activities decrease in serum and the CSF, while, surprisingly, the serum Cu/ZnSOD activity increases after the MX therapy. We also noted a marked decrease in CSF CAT and GSH-Px activity after the MX treatment. Our results strongly suggest the influence of MX therapy on oxidation/antioxidation status of serum and the CSF. These findings open up new opportunities for a better understanding of underlying physiopathological events in MS and provide a new insight into MX's mechanisms of action, especially its potent side effects.