Critical thermal maxima and oxygen uptake in Elysia viridis, a sea slug that steals chloroplasts to photosynthesize.

Photosynthetic animals produce oxygen, providing an ideal lens for studying how oxygen dynamics influence thermal sensitivity. The algivorous sea slug Elysia viridis can steal and retain chloroplasts from the marine alga Bryopsis sp. for months when starved, but chloroplast retention is mere weeks when they are fed another green alga, Chaetomorpha sp. To examine plasticity in thermal tolerance and changes in net oxygen exchange when fed and starving, slugs fed each alga were acclimated to 17°C (the current maximum temperature to which they are exposed in nature) and 22°C (the increase predicted for 2100) and measured at different points during starvation. We also examined increased illumination to evaluate a potential tradeoff between increased oxygen production but faster chloroplast degradation. Following acclimation, we subjected slugs to acute thermal stress to determine their thermal tolerance. We also measured net oxygen exchange before and after acute thermal stress. Thermal tolerance improved in slugs acclimated to 22°C, indicating they can acclimate to temperatures higher than they naturally experience. All slugs exhibited net oxygen uptake, and rates were highest in recently fed slugs before exposure to acute thermal stress. Oxygen uptake was suppressed following acute thermal stress. Under brighter light, slugs exhibited improved thermal tolerance, possibly because photosynthetic oxygen production alleviated oxygen limitation. Accordingly, this advantage disappeared later in starvation when photosynthesis ceased. Thus, E. viridis can cope with heatwaves by suppressing metabolism and plastically adjusting heat tolerance; however, starvation influences a slug's thermal tolerance and oxygen uptake such that continuous access to algal food for its potential nutritive and oxygenic benefits is critical when facing thermal stress.

Integrative Approaches to Understanding Organismal Responses to Aquatic Deoxygenation.

AbstractOxygen bioavailability is declining in aquatic systems worldwide as a result of climate change and other anthropogenic stressors. For aquatic organisms, the consequences are poorly known but are likely to reflect both direct effects of declining oxygen bioavailability and interactions between oxygen and other stressors, including two-warming and acidification-that have received substantial attention in recent decades and that typically accompany oxygen changes. Drawing on the collected papers in this symposium volume ("An Oxygen Perspective on Climate Change"), we outline the causes and consequences of declining oxygen bioavailability. First, we discuss the scope of natural and predicted anthropogenic changes in aquatic oxygen levels. Although modern organisms are the result of long evolutionary histories during which they were exposed to natural oxygen regimes, anthropogenic change is now exposing them to more extreme conditions and novel combinations of low oxygen with other stressors. Second, we identify behavioral and physiological mechanisms that underlie the interactive effects of oxygen with other stressors, and we assess the range of potential organismal responses to oxygen limitation that occur across levels of biological organization and over multiple timescales. We argue that metabolism and energetics provide a powerful and unifying framework for understanding organism-oxygen interactions. Third, we conclude by outlining a set of approaches for maximizing the effectiveness of future work, including focusing on long-term experiments using biologically realistic variation in experimental factors and taking truly cross-disciplinary and integrative approaches to understanding and predicting future effects.

Photosynthate accumulation in solar-powered sea slugs - starving slugs survive due to accumulated starch reserves.

BACKGROUND: Solar-powered sea slugs are famed for their ability to survive starvation due to incorporated algal chloroplasts. It is well established that algal-derived carbon can be traced in numerous slug-derived compounds, showing that slugs utilize the photosynthates produced by incorporated plastids. Recently, a new hypothesis suggests that the photosynthates produced are not continuously made available to the slug. Instead, at least some of the plastid's photosynthetic products are stored in the plastid itself and only later become available to the slug. The long-term plastid-retaining slug, Elysia timida and its sole food source, Acetabularia acetabulum were examined to determine whether or not starch, a combination of amylose and amylopectin and the main photosynthate produced by A. acetabulum, is produced by the stolen plastids and whether it accumulates within individual kleptoplasts, providing an energy larder, made available to the slug at a later time. RESULTS: Histological sections of Elysia timida throughout a starvation period were stained with Lugol's Iodine solution, a well-known stain for starch granules in plants. We present here for the first time, an increase in amylose concentration, within the slug's digestive gland cells during a starvation period, followed by a sharp decrease. Chemically blocking photosynthesis in these tissues resulted in no observable starch, indicating that the starch in untreated animals is a product of photosynthetic activity. CONCLUSION: This suggests that kleptoplasts function as both, a nutritive producer and storage device, holding onto the polysaccharides they produce for a certain time until they are finally available and used by the starving slug to withstand extended starvation periods.