RESUMO
H2O2 intensifies CN(-)-induced apoptosis in stoma guard cells and to lesser degree in basic epidermal cells in peels of the lower epidermis isolated from pea leaves. The maximum effect of H2O2 on guard cells was observed at 10(-4) M. By switching on non-cyclic electron transfer in chloroplasts menadione and methyl viologen intensified H2O2 generation in the light, but prevented the CN--induced apoptosis in guard cells. The light stimulation of CN- effect on guard cell apoptosis cannot be caused by disturbance of the ribulose-1,5-bisphosphate carboxylase function and associated OH* generation in chloroplasts with participation of free transition metals in the Fenton or Haber-Weiss type reactions as well as with participation of the FeS clusters of the electron acceptor side of Photosystem I. Menadione and methyl viologen did not suppress the CN(-)-induced apoptosis in epidermal cells that, unlike guard cells, contain mitochondria only, but not chloroplasts. Quinacrine and diphenylene iodonium, inhibitors of NAD(P)H oxidase of cell plasma membrane, had no effect on the respiration and photosynthetic O2 evolution by leaf slices, but prevented the CN(-)-induced guard cell death. The data suggest that NAD(P)H oxidase of guard cell plasma membrane is a source of reactive oxygen species (ROS) needed for execution of CN(-)-induced programmed cell death. Chloroplasts and mitochondria were inefficient as ROS sources in the programmed death of guard cells. When ROS generation is insufficient, exogenous H2O2 exhibits a stimulating effect on programmed cell death. H2O2 decreased the inhibitory effects of DCMU and DNP-INT on the CN(-)-induced apoptosis of guard cells. Quinacrine, DCMU, and DNP-INT had no effect on CN(-)-induced death of epidermal cells.
Assuntos
Apoptose , Cianetos/toxicidade , Peróxido de Hidrogênio/toxicidade , Folhas de Planta/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Ácido Ascórbico/metabolismo , Ácido Ascórbico/farmacologia , Membrana Celular/metabolismo , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Cloroplastos/efeitos dos fármacos , Cloroplastos/metabolismo , Cianetos/metabolismo , Diurona/metabolismo , Diurona/farmacologia , Sinergismo Farmacológico , Transporte de Elétrons/efeitos dos fármacos , Inibidores Enzimáticos/metabolismo , Inibidores Enzimáticos/farmacologia , Peróxido de Hidrogênio/metabolismo , Microscopia de Fluorescência , NADPH Oxidases/metabolismo , Pisum sativum/citologia , Pisum sativum/metabolismo , Complexo de Proteína do Fotossistema I/metabolismo , Epiderme Vegetal/citologia , Epiderme Vegetal/metabolismo , Folhas de Planta/metabolismo , Espécies Reativas de Oxigênio , Trinitrobenzenos/metabolismo , Trinitrobenzenos/farmacologiaRESUMO
Received Revision received We investigated the CN--induced apoptosis of guard cells in epidermal peels isolated from pea (Pisum sativum L.) leaves. This process was considerably stimulated by illumination and suppressed by the herbicides DCMU (an inhibitor of the electron transfer between quinones Q(A) and Q(B) in PS II) and methyl viologen (an electron acceptor from PS I). These data favor the conclusion drawn by us earlier that chloroplasts are involved in the apoptosis of guard cells. Pea mutants with impaired PS I (Chl-5), PS II (Chl-I), and PS II + PS I (Xa-17) were tested. Their lesions were confirmed by the ESR spectra of Signal I (oxidized PS I reaction centers) and Signal II (oxidized tyrosine residue Y(D) in PS II). Destruction of nuclei (a symptom of apoptosis) and their consecutive disappearance in guard cells were brought about by CN- in all the three mutants and in the normal pea plants. These results indicate that the light-induced enhancement of apoptosis of guard cells and its removal by DCMU are associated with PS II function. The effect of methyl viologen preventing CN--induced apoptosis in wild-type plants was removed or considerably decreased upon the impairment of the PS II and/or PS I activity.
Assuntos
Apoptose/fisiologia , Cloroplastos/fisiologia , Fotossíntese/fisiologia , Complexo de Proteína do Fotossistema I/fisiologia , Complexo de Proteína do Fotossistema II/fisiologia , Núcleo Celular/genética , Núcleo Celular/fisiologia , Cloroplastos/genética , Diurona/toxicidade , Espectroscopia de Ressonância de Spin Eletrônica , Mutação , Paraquat/toxicidade , Pisum sativum/genética , Pisum sativum/fisiologia , Fotossíntese/genética , Complexo de Proteínas do Centro de Reação Fotossintética , Complexo de Proteína do Fotossistema I/genética , Complexo de Proteína do Fotossistema II/genética , Folhas de Planta/genética , Folhas de Planta/fisiologiaRESUMO
The effect of cyanide, an apoptosis inducer, on pea leaf epidermal peels was investigated. Illumination stimulated the CN--induced destruction of guard cells (containing chloroplasts and mitochondria) but not of epidermal cells (containing mitochondria only). The process was prevented by antioxidants (alpha-tocopherol, 2,5-di-tret-butyl-4-hydroxytoluene, and mannitol), by anaerobiosis, by the protein kinase C inhibitor staurosporine, and by cysteine and serine protease inhibitors. Electron acceptors (menadione, p-benzoquinone, diaminodurene, TMPD, DCPIP, and methyl viologen) suppressed CN--induced apoptosis of guard cells, but not epidermal cells. Methyl viologen had no influence on the removal of CN--induced nucleus destruction in guard cells under anaerobic conditions. The light activation of CN--induced apoptosis of guard cells was suppressed by DCMU (an inhibitor of the electron transfer in Photosystem II) and by DNP-INT (an antagonist of plastoquinol at the Qo site of the chloroplast cytochrome b6f complex). It is concluded that apoptosis initiation in guard cells depends on the simultaneous availability of two factors, ROS and reduced quinones of the electron transfer chain. The conditions for manifestation of programmed cell death in guard and epidermal cells of the pea leaf were significantly different.
Assuntos
Apoptose/fisiologia , Cloroplastos/fisiologia , Pisum sativum/fisiologia , Núcleo Celular/fisiologia , Cianetos/metabolismo , Diurona/farmacologia , Herbicidas/farmacologia , Inibidores de Proteases/farmacologia , Proteína Quinase C/farmacologia , Trinitrobenzenos/farmacologiaRESUMO
This paper reviews data on programmed cell death (apoptosis) in animals and plants. Necrosis is a pathological scenario of cell death, which entails an inflammatory response in animal tissues. Apoptosis results in the disintegration of animal/plant cells into membrane vesicles enclosing the intracellular content, which are thereupon engulfed by adjacent or specialized cells (phagocytes) in animals. Plants lack such specialized cells, and plant cell walls prevent phagocytosis. The paper considers the main molecular mechanisms of apoptosis in animals and the pathways of activation of caspases, evolutionarily conserved cysteine proteases. A self-contained section concerns itself with the process of programmed cell death (PCD) in microorganisms including: 1) cell death in the myxomycete Dictyostelium discoideum and the parasitic flagellate Trypanosoma cruzi; 2) PCD in genetically manipulated yeast expressing the proapoptotic Bax and Bak proteins; 3) the death of a part of a prokaryotic cell population upon the depletion of nutrient resources or under stress; 4) the elimination of cells after a loss of a plasmid encoding a stable cytotoxic agent in combination with an unstable antidote; and 5) PCD in phage-infected bacterial cells.
Assuntos
Apoptose/fisiologia , Animais , Bactérias/citologia , Dictyostelium/citologia , Necrose , Células VegetaisRESUMO
Degradation of nuclei in epidermal and guard cells of pea leaves was induced by NaCN. Guard cells were considerably more resistant to CN- than epidermal cells. CN--induced nucleus degradation in guard cells was accelerated by illumination. The effect of illumination was negligible in epidermal cells that, unlike guard cells, do not contain chloroplasts. These data may indicate a role of chloroplasts in CN--induced cell death. CN--induced nucleus degradation in epidermal cells was retarded by antioxidants (butylated hydroxytoluene and vitamin E). The effect of CN- in guard cells was largely removed by vitamin E. Salicylic acid, an inhibitor of catalase and ascorbate peroxidase, induced 100% degradation of nuclei in epidermal cells but did not significantly affect nuclei in guard cells. CN--induced inhibition of catalase and peroxidase is assumed to lead to generation and accumulation of reactive oxygen species inducing apoptosis. Like mitochondria, which play an important role in animal cell apoptosis, chloroplasts may take part in apoptosis in plant cells.