A novel indel within the bovine SEPT7 gene is associated with ovary length.

The ovary can generate oocytes and secrete female hormones and thus is of great significance to animal fertility. In turn, the functioning of this organ has an effect on the profit margins of the livestock breeding industry. As the development-regulating gene and target gene of miR-202, SEPT7 might play an important role in ovarian growth. Therefore, we hypothesized that SEPT7 is related to ovarian traits owing to the regulation of gonad-specific miR-202. To further investigate the connection between bovine SEPT7 and ovarian development, we analyzed data from 408 samples. After genotyping and analyzing three selected loci, we found that two out of the three loci (L1 and L5) were polymorphic, of which the minimum allelic frequencies were 0.417 (L1) and 0.094 (L5). Moreover, one novel indel L1 of SEPT7 was associated with ovarian length (p < 0.05). More specifically, individuals with II and ID genotypes have longer ovaries than those with the DD genotype. Our work shows that SEPT7 can be selected as a testing marker gene for animal fertility. Our findings contribute to improving the prospects of the cattle industry and the wider use of genetic techniques in breeding.

Two Novel Rare Strongly Linked Missense SNPs (P27R and A85G) Within the GDF9 Gene Were Significantly Associated With Litter Size in Shaanbei White Cashmere (SBWC) Goats.

Growth differentiation factor 9 (GDF9) is a high-fertility candidate gene that plays a crucial role in early folliculogenesis in female mammals. In this study, direct sequencing was used to screen possible SNP loci in the goat GDF9 gene. Three SNP loci, p.proline27alanine (P27R), p.leucine61leucine (L61L), and p.alanine85glycine (A85G), were identified in Shaanbei white cashmere (SBWC) goats. Among the three SNPs, two rare missense SNP loci (P27R and A85G) were discovered to be strongly linked with each other (D' value = 0.926, r 2 value = 0.703). Both P27R and A85G loci had two genotypes: wild type and heterozygous type. A85G exerted a significant effect on litter size (P = 0.029) in SBWC goats, and the heterozygous genotype was superior in comparison with the wild type. The heterozygous genotype was also superior in P27R but no significant association was found. However, the combination genotypes of P27R and A85G were identified to have superior effects on litter size (P = 3.8E-15). This information suggested that these two SNPs influenced litter size in goats synergistically. Combining this information with our previous studies, we propose that the GDF9 gene is the principal high-fertility candidate gene and that the A85G locus is a promising SNP that affects litter size in goats. These results may fill a research gap regarding rare mutations as well as provide crucial molecular markers that could be useful in marker-assisted selection (MAS) goat rearing when selecting superior individuals.

Chlorpyrifos inhibits sperm maturation and induces a decrease in mouse male fertility.

BACKGROUND: Pesticides, especially organophosphorus pesticides such as chlorpyrifos (CPF), play an important role in modern agriculture. Studies have shown that pesticide residues are an important cause of male reproductive injury in mammal. AIM: The aim of this study was to evaluate the reproductive damage caused by CPF in male mice and investigate the underlying mechanisms. METHODS: In vivo, C57BL/6 mice (6-8 weeks old) were treated with CPF for 14, 70, and 80 days by intraperitoneal injection, intragastric administration, and dietary supplementation, respectively. Then, sperm from the cauda epididymidis was cultured in vitro to confirm the deleterious effects of CPF. RESULTS: The in vivo results indicated that, after treatment with CPF by dietary supplementation and intraperitoneal injection, the expression of reproduction-related genes in the mouse testes was altered, although the mice were fertile and the testes presented no morphological abnormalities. Notably, mating experiments revealed that the fertility of male mice was decreased following CPF administration by gavage. Sperm motility within the cauda epididymidis declined significantly after CPF treatment, which was accompanied by a decrease in sperm density, upregulation of relative reactive oxygen species (ROS) levels, and downregulation of glutathione reductase activity. In vitro incubation experiments showed that sperm rapidly lost their capacity for linear movement; the relative ROS levels also increased significantly, while the mitochondrial membrane potential (MMP) showed a significant decrease. However, the integrity of the plasma membrane was not affected by CPF administration. CONCLUSIONS: The above data indicated that exposure to CPF reduces sperm motility by disrupting mitochondrial function and increasing the level of oxidative stress during sperm maturation, thereby reducing the fecundity of male mice.